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| 1 | DNA immunization with fusion genes encoding different regions of hepatitis C virus E2 fused to the gene for hepatitis B surface antigen elicits immune responses to both HCV and HBV显示文摘AIM: Both Hepatitis B virus (HBV) and Hepatitis C virus(HCV) are major causative agents of transfusion-associatedand community-acquired hepatitis worldwide. Developmentof a HCV vaccine as well as more effective HBV vaccines isan urgent task. DNA immunization provides a promisingapproach to elicit protective humoral and cellular immuneresponses against viral infection. The aim of this study is toachieve immune responses against both HCV and HBV by DNAimmunization with fusion constructs comprising various HCVE2 gene fragments fused to HBsAg gane of HBV.METHODS: C57BL/6 mice were immunized with plasmid DNAexpressing five fragments of HCV E2 fused to the gene forHBsAg respectively. After one primary and one boostingimmunizations, antibodies against HCV E2 and HBsAg weretested and subtyped in ELISA. Splenic cytokine expressionof IFN-γ and IL-10 was analyzed using an RT-PCR assay.Post-immune mouse antisera also were tested for theirability to capture HCV viruses in the serum of a hepatitis Cpatient in vitro.RESUTLTS: After immunization, antibodies against bothHBsAg and HCV E2 were detected in mouse sera, withIgG2a being the dominant immunoglobulin sub-class. High-level expression of INF-γ was deuetected in cultured splenic cells.Mouse antisera against three of the five fusion constructs wereable to capture HCV viruses in an in vitro assay.CONCLUSION: The results indicate that these fusionconstructs could efficiently elicit humoral and Th1 dominantcellular immune responses against both HBV S and HCV E2antigens in DNA-immunized mice. They thus could serve ascandidates for a bivalent vaccine against HBV and HCVinfection. In addition, the capacity of mouse antisera againstthree of the five fusion constnucts to capture HCV virusses invitro suggested that neutralizing epitopes may be present inother regions of E2 besides the hypervariable region 1. | JinJ YangJY 等 | 2002 | World Journal of Gastroenterology2002，8，3： | 18 |
| 2 | Three-dimensional bio-printing显示文摘Three-dimensional(3D) printing technology has been widely used in various manufacturing operations including automotive, defence and space industries. 3D printing has the advantages of personalization, flexibility and high resolution, and is therefore becoming increasingly visible in the high-tech fields. Three-dimensional bio-printing technology also holds promise for future use in medical applications. At present 3D bio-printing is mainly used for simulating and reconstructing some hard tissues or for preparing drug-delivery systems in the medical area. The fabrication of 3D structures with living cells and bioactive moieties spatially distributed throughout will be realisable. Fabrication of complex tissues and organs is still at the exploratory stage. This review summarize the development of 3D bio-printing and its potential in medical applications, as well as discussing the current challenges faced by 3D bio-printing. | GU Qi HAO Jie LU YangJie WANG Liu WALLACE Gordon G. ZHOU Qi | 2015 | Science China(Life Sciences)2015，58，5： | 11 |
| 3 | Improved Denitrification of Municipal Sludge in Biofilm-electrode Reactor显示文摘The denitrification of municipal sludge was improved by combining biofilm process with the electro-chemical effect in a single novel reactor. Experiments in this reactor[electric current 60 mA, hydraulic retention time(HRTs) 6.0 h] showed that the removal of COD_ Cr, ammonia nitrogen and total nitrogen in the biofilm-electrode reactor were 2.5%, 1.2%, 14.9%, respectively, higher than those in a traditional biofilm reactor. | ZHANGLe-hua JIAJin-ping WANGYa-lin YANGJi | 2004 | Chemical Research in Chinese Universities2004，20，4： | 3 |
| 4 | Hercynian-Indosinian Sedimento-Tectonic Evolution of the Nanpanjiang Basin显示文摘Following the orogeny in the Early Palaeozoic, the tectonic evolution of the Nanpanjiang basin in the Hercynian-Indosinian stage constituted a complete Wilson Cycle, made up of the stages of early rifting (D-P1), late rifting and passive margin developement (P2-T1), and peripheral foreland basin developement (T2-T3). The REE data of the initial Early Triassic basalts from the southern part of the basin has verified the existence of the oceanic crust once in the basinal evolution. The basin developed into a foreland one in the Middle-Late Triassic, indicating that the Yangtze plate once collided with the Indo-Sinian plate along the Black River fracture zone. | Qin Jianhua, Wu Yinglin, Yan Yangji and Zu ZhongfaResearch Center of Sedimentary and Energy Geology, Chinese Academy of Geological Sciences, Chengdu, Sichuan | 1996 | Acta Geologica Sinica(English Edition)1996，70，4： | 3 |
| 5 | Model update mechanism for mean-shift tracking显示文摘In order to solve the model update problem in mean-shift based tracker, a novel mechanism is proposed. Kalman filter is employed to update object model by filtering object kernel-histogram using previous model and current candidate. A self-tuning method is used for adaptively adjust all the parameters of the filters under the analysis of the filtering residuals. In addition, hypothesis testing servers as the criterion for determining whether to accept filtering result. Therefore, the tracker has the ability to handle occlusion so as to avoid over-update. The experimental results show that our method can not only keep up with the object appearance and scale changes but also be robust to occlusion. | PengNingsong YangJie LiuErqi | 2005 | Journal of Systems Engineering and Electronics2005，16，1： | 3 |
| 6 | Bolt insertion damage and mechanical behaviors investigation of CFRP/CFRP interference fit bolted joints显示文摘Interference fit has advantages in improving fatigue behaviors of composite bolted joints,however, interference fit bolt insertion tends to cause damages in laminates weakening joint mechanical properties. Therefore, an experimental study was conducted to investigate bolt insertion damages of Carbon Fiber Reinforced Polymer(CFRP)/CFRP interference fit bolted joints.Mechanical behaviors of joints were also evaluated experimentally under both quasi-static loads and cyclic loads. Scanning Electron Microscope(SEM) and high-resolution X-ray micro-CT scan were used to examine micro damages in laminates. Damage and failure behaviors of joints were characterized. The results demonstrated that the hole entrance in upper laminate and the laminate boundary near the hole wall were the most critical regions for damages during bolt insertions. However, the influence of those damages on quasi-static failure loads and fatigue failure modes of joints was minimal. Delamination and matrix cracking occurred first in laminates following fiber and matrix fracture in quasi-static tensile tests. Interference fit could improve the fatigue resistance of the laminate hole, however, the bolt seemed to suffer a more critical local fatigue loading condition.This paper can contribute to composite structure designs, especially in understanding damage and failure behaviors of composite bolted joints. | Yangjie ZUO Ting YUE Ruisong JIANG Zengqiang CAO Liu YANG | 2022 | Chinese Journal of Aeronautics2022，35，9： | 3 |
| 7 | Synthesis of the superfine high-entropy zirconate nanopowders by polymerized complex method显示文摘The high-purity and superfine high-entropy zirconate nanopowders,namely(Y_(0.25)La_(0.25)Sm_(0.25)Eu_(0.25))_(2)Zr_(2)O_(7)nanopowders,without agglomeration,were successfully synthesized via polymerized complex method at low temperatures for the first time.The results showed that the crystallinity degree,lattice strain,and particle size of the as-synthesized powders were gradually enhanced with the increase of the synthesis temperature from 800 to 1300℃.The as-synthesized powders involved fluorite phase in the range of 800-1200℃while they underwent the phase evolution from fluorite to pyrochlore at 1300℃.It is worth mentioning that the as-synthesized powders at 900℃are of the highest quality among all the as-synthesized powders,which is due to the fact that they not only possess the particle size of 11 nm without agglomeration,but also show high purity and good compositional uniformity. | Yangjie HAN Renwang YU Honghua LIU Yanhui CHU | 2022 | Journal of Advanced Ceramics2022，11，1： | 2 |
| 8 | Research and application of multi-angle polarization characteristics of water body mirror reflection显示文摘On the basis of the multi-angle polarized reflection spectrum of the water samples,the water body mirror reflection polarization characteristics and mechanism are described systematically. By altering such influential factors as the angle of incidence,detecting angle,detecting azimuth angle and polari-zation angle,ubiquitous laws for the multi-angle polarized reflection spectrum of the water samples are obtained. Combining multi-angle remote sensing with polarized light,the multi-angle polarized reflec-tion method about eliminating the water body mirror reflection and the suitable time of the polarized remote sensing of the water body are proposed. This study provides technical references for the ap-plication of multi-angle polarization technology on water body remote sensing. | LUO YangJie ZHAO YunSheng LI XiaoWen WU TaiXia ZHAO LiLi | 2007 | Science China Earth Sciences2007，50，6： | 2 |
| 9 | Cyclopalladated ferrocenylimines as efficient catalysts for homogeneous catalysis:A brief introduction to our preliminary achievements显示文摘Cyclopalladated ferrocenylimines as a novel kind of highly efficient palladium catalyst precursors, which are air-stable and easy to handle, have been successfully applied in a wide variety of catalytic reactions ranging from classical reactions, such as Heck, Suzuki, Sonogashira and Buchwald-Hartwig couplings, to cyanation, addition reactions of arylboronic acids, and coupling reactions involving terminal alkynes. The preliminary achievements regarding their applications to homogeneous catalysis are concisely described herein. | WU YangJie YANG Fan ZHANG JinLi CUI XiuLing GONG JunFang SONG MaoPing LI TieSheng | 2010 | Chinese Science Bulletin2010，55，25： | 2 |
| 10 | Global shape reconstruction of nano grid with singly fixed camera显示文摘Depth from defocus(DFD),as a typical shape reconstruction method,has been widely researched in most recent years.However,all the existing DFD algorithms require at least two defocused images with different camera parameters.Unfortunately,in micro/nano manipulation,any change on visual sensor's parameters is absolutely forbidden.Therefore,a novel DFD method to reconstruct the shape of a nano grid on micro/nano scale is researched in this paper.First,the blurring imaging model is constructed with the relative blurring and the diffusion equation.Second,the relationship between depth and blurring is discussed from four aspects.Subsequently,depth measurement problem is transformed into an optimization issue which is solved using the gradient flow algorithm.Finally,experiment results and error analysis are conducted to show the feasibility and effectiveness of the proposed method. | WEI YangJie WU ChengDong DONG ZaiLi | 2011 | Science China(Technological Sciences)2011，54，4： | 2 |
| 11 | Synthesis of Symmetrical Biaryls via Rhodium Catalyzed Dimerization of Arylmercurials and Mechanism of the Dimerization显示文摘A series of symmetrical biaryls was synthesized from arylmercuric chlorides and biarylmercurials in the presence of [ClRh(CO) 2] 2 in hexamethylphosphoramide(HMPA). The mechanism of the [ClRh(CO) 2] 2 catalyzed dimerization of biarylmercurials was studied, and shown to be mainly intermolecular reaction. | WANG Yulan WU Yangjie | 2000 | Chemical Research in Chinese Universities2000，16，2： | 2 |
| 12 | Modeling of nano piezoelectric actuator based on block matching algorithm with optimal block size显示文摘In order to model the hysteresis behavior of a nano piezoelectric actuator(PA)on nano scale in a real time system,a new hysteresis modeling method based on an improved sub-pixel blocking matching algorithm with an optimal block size is proposed in this paper.First,Preisach model is introduced to model the hysteresis behavior of a piezoelectric actuator.Then,a real time block matching algorithm is researched and its block size is optimized with a standard object.Finally,experiments are performed with respect to a nanometer movement platform system,and the results show the feasibility and validity of the sub-pixel estimation based block matching algorithm and its application in modeling the hysteresis behavior of PA. | WEI YangJie WU ChengDong | 2013 | Science China(Technological Sciences)2013，56，11： | 2 |
| 13 | Variational Bayesian method for speech enhancement显示文摘 | Huang Qing-hua YangJie and Zhou Yue | 2007 | Neurocompuiinq2007，70，1618： | 1 |
| 14 | Facies distribution and retreat of Middle Triassic Platform margin, Guizhou province, South China显示文摘 | Enos P Wei Jiayong Yah Yangji | 1997 | Sedimentary1997，44，3： | 1 |
| 15 | A Clinical experienceof treating exfoliative wounds using nanocrystalline silvercontaining dressings Acticoa显示文摘 | YangJY Huang CY’Chuang SS | 2007 | Bums2007，33，6： | 1 |
| 16 | IntelligentfaultdiagnosisofrollingelementbearingbasedonSVMsandfractaldimension显示文摘 | YangJY ZhangYY ZhuYS | 2007 | MechanicalSystemandSignalProcessing2007，21，5： | 1 |
| 17 | Occurrence of phthalate esters in water and sediment of urban lakes in a subtropical city, Guangzhou, South China显示文摘 | Feng Zeng Kunyan Cui Zhiyong Xie Min Liu Yangjie Li Yujun Lin Zunxiang Zeng Fangbai Li | 2007 | Environment International2007，，3： | 1 |
| 18 | Activation of murine double minute 2 by Akt in mammary epithelium delays mammary involution and accelerates mammary tumorigenesis显示文摘 | Cheng X Xia W YangJY | 2010 | Cancer Res2010，70，19： | 1 |
| 19 | Process forthe production of tilapia retorted skin gelatin hydrolysates with optimized antioxidative properties显示文摘 | YangJI LiangW S ChowCJ | | 0，，10： | 1 |
| 20 | Tri- pod - like Compounds : Syntheses of tris ( p - OR o - Amino Phe- noxymethyl) ,tris( p - OR o - Amino Phcnoxyethyl) Amine, and their Schiff Base or Salicyl Derivatives 显示文摘 | Huijie Lu Yaoting Fan Qinghuan Zhao and Yangjie Wu | 2001 | Synthetic Communica- tions2001，31，10： | 1 |
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