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| 1 | Identification of an HLA-A~* 0201-restricted CD8^+ T-cell epitope SSp-1 of SARS-CoV spike protein显示文摘A novel coronavirus, severe acute respiratory syndrome (SA RS)-associated coronavirus (SARS-CoV), has been identified as the causal agent of SARS. Spike (S) protein is a major structural glycoprotein of the SARS virus and a potential target for SARS-specific cell-mediated immune responses. A pa nel of S protein-derived peptides was tested for their binding affinity to HLA -A *0201 molecules. Peptides with high affinity for HLA-A *0201 were then as se ssed for their capacity to elicit specific immune responses mediated by cytotoxi c T lymphocytes (CTLs) both in vivo, in HLA-A2.1/K b transgenic mice, a nd in vitro, from peripheral blood lymphocytes (PBLs) harvested from healthy HLA-A 2.1 + donors. SARS-CoV protein-derived peptide-1 (SSp-1 RLNEVAKNL), induced pepti de-specific CTLs both in vivo (transgenic mice) and in vitro (human PBL s), which specifically released interferon-gamma (IFN-gamma) upon stimulation with SSp-1-pulsed autologous dendritic cells (DCs) or T2 cells. SSp-1-specif ic CTLs also lysed major histocompatibility complex (MHC)-matched tumor cell lines engineered to express S proteins. HLA-A *0201-SSp-1 tetramer staining re vealed the presence of significant populations of SSp-1-specific CTLs in SSp- 1-induced CD8 + T cells. We propose that the newly identified epitope SSp-1 w ill help in the characterization of virus control mechanisms and immunopathology in SARS-CoV infection, and may be relevant to the development of immunotherape utic approaches for SARS. | WangB ChenH JiangX ZhangM WanT LiN ZhouX WuY YangF YuY WangX YangR CaoX | 2004 | 第二军医大学学报2004，25，9： | 21 |
| 2 | 显示文摘 | JUHX YANGF CHEN F etal | 1999 | Electroanal1999，11，1： | 1 |
| 3 | Effectsofsalvianolicacidsonoxi-dativestressandhepaticfibrosisinrats显示文摘 | WangSB TianS YangF etal | 2010 | ToxicolApplPharma-col2010，242，2： | 1 |
| 4 | Experiments and theory in strain gradient elasticity 显示文摘 | Lain D C C YangF Chong A C M | 2003 | Journal of the Mechanics and Physics of Solids2003，51，： | 1 |
| 5 | Renoprotectiveeffectsofberberineanditspossiblemolecularmechanismsincombinationofhigh-fatdietand low-dosestreptozotocin-induced diabeticrats显示文摘 | WangFL TangLQ YangF etal | 2013 | MolBiolRep2013，40，3： | 1 |
| 6 | Treatment with a neutralizing anti-murine IL-17 antibody after the onset of coxsaehievirus B3 -induced viral myocarditis reduces myocardium inflammation显示文摘 | YANGF WU W F YAN Y L | 2011 | Virol J2011，8，1： | 1 |
| 7 | AsimpleandefficientmethodforpurificationofprawnbaculovirusDNA显示文摘 | YangF WangW ChenRZ etal | 1997 | JournalofVirologicalMeghods1997，67，： | 1 |
| 8 | Effect of procine bone morphognetic protein on healing of bone defcet in the rabbit radius显示文摘 | Yangf KH Lee HM | 1992 | Yonsei Med J1992，33，1： | 1 |
| 9 | ThermalconductivitiesofYSZ/Al2O3composites显示文摘 | YangF Zhao X XiaoP | 2010 | JournaloftheEuropeanCeGramicSociety2010，30，： | 1 |
| 10 | Schafer AI Hy- perbomocysteinemia accelerates atherosclerosis in cystathionine beta - synthase and apoli- peprotein E double knock -out mice with and without 显示文摘 | Wang H Jiang X YangF | 2003 | dietary perturbation2003，12，1： | 1 |
| 11 | ThermalconductivitiesofYSZ/Al2O3composites显示文摘 | YangF Zhao X XiaoP | 2010 | JournaloftheEuropeanCeGramicSociety2010，30，： | 1 |
| 12 | Platelets as immune mediators: their role in host defense responses and sepsis显示文摘 | LiZ YangF DunnS | 2011 | Thromb Res2011，127，3： | 1 |
| 13 | H control for networked systems with random communication delays 显示文摘 | YangF W Wang Z D Hung Y S | 2006 | 1EEE Transactions on Automatic Control2006，51，3： | 1 |
| 14 | Multi-scaleanalysisofthewaterresourcescarryingcapacityoftheLiaoheBasinbasedonecologicalfootprints显示文摘 | WangS YangF XuLL etal | 2013 | JournalofCleanerProduction2013，，15： | 1 |
| 15 | Coxsackievirus B5,associated with neurological hand,foot and mouth disease,China显示文摘 | HuYF YangF DuJ | 2012 | Infect2012，65，18： | 1 |
| 16 | Novel heat shock protein Hsp70L1 activates dendritic cells and acts as a Th1 polarizing adjuvant显示文摘Heat shock proteins (HSPs) are reported to act as effective adjuvants to elicit anti-tumor and anti-infection immunity. Here, we report that Hsp70-like protein 1 (Hsp70L1), a novel HSP derived from human dendritic cells (DCs), has potent adjuvant effects that polarize responses toward Th1. With a calculated molecular weight of 54.8 kDa, Hsp70L1 is smaller in size than Hsp70 but resembles it both structurally and functionally. Hsp70L1 shares common receptors on DCs with Hsp70 and can interact with DCs, promoting DC maturation and stimulating secretion of the proinflammatory cytokines interleukin 12p70 (IL-12p70), IL-1beta, tumor necrosis factor-alpha (TNF-alpha), and the chemokines IP-10, macrophage inflammatory protein-1alpha (MIP-1alpha), MIP-1beta, and normal T cell expressed and secreted (RANTES). The induction of interferon-gamma-inducible protein 10 (IP-10) secretion by Hsp70L1 is not shared by Hsp70, and other functional differences include more potent stimulation of DC IL-12p70, CC-chemokine, and CCR7 and CXCR4 expression by Hsp70L1. Immunization of mice with the hybrid peptide Hsp70L1-ovalbumin(OVA)(257-264) induces an OVA(257-264)-specific Th1 response and cytotoxic T lymphocyte (CTL) that results in significant inhibition of E.G7-OVA tumor growth. The ability of Hsp70L1 to activate DCs indicates its potential as a novel adjuvant for use with peptide immunizations; the Hsp70L1 antigen peptide hybrid may serve as a more effective vaccine for the control of cancer and infectious diseases. | WanT ZhouX ChenG AnH ChenT ZhangW LiuS JiangY YangF WuY CaoX | 2005 | 第二军医大学学报2005，26，7： | 1 |
| 17 | Electrospinningofnano/microscalepo-ly(L-lacticacid)alignedfibersandtheirpotentialinneuraltissueengineering显示文摘 | YangF WangS | 2005 | EuropeanPolymerJournal2005，26，15： | 1 |
| 18 | Hepatitis B virus infection in hepatocellular carcinoma tissues upregulates expression of DNA methyltransferases 显示文摘 | LiH YangF GaoB | 2015 | Int J Clin Exp Med2015，8，41： | 1 |
| 19 | Complete genome analysis of coxsackievirus A2,A4,A5,and A10 strains isolated from hand, foot and mouth disease patients in China revealing frequent recombination of human enterovirus A 显示文摘 | HuYF YangF DuJ | 2011 | J Clin Microbiol2011，49，24： | 1 |
| 20 | DecreasedlevelsofCD44proteinandmRNAinprostatecarcinoma,correlationwithtumorgradeandploidy显示文摘 | KallakuryBV YangF FiggeJ etal | 1996 | Cancer1996，78，： | 1 |
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