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| 1 | Interplay of neuropilin-1 and semaphorin 3A after partial hepatectomy in rats显示文摘AIM:To elucidate the role of neuropilin-1(Nrp-1) and semaphorin 3A(Sema3A) in sinusoidal remodeling during liver regeneration in rats.METHODS:Male Wistar/ST rats at 7 wk of age,weighing about 200 g,were used for all animal experiments.In vivo,at 24,48,72,96,144 and 192 h after twothirds partial hepatectomy(PHx),the remnant livers were removed.Liver tissues were immunohistochemically stained for Nrp-1,Sema3A and SE-1,a liver sinusoidal endothelial cell(SEC) marker.Total RNA of the liver tissue was extracted and reversely transcribed into cDNA.The mRNA expression of Sema3A was analyzed by quantitative real-time polymerase chain reaction and normalized to that of ribosomal protein S18.In vitro,SECs were isolated from rat liver and cultured in endothelial growth medium containing 20 ng/mL vascular endothelial cell growth factor.Migration of SECs in primary culture was assessed by cell transwell assay with or without recombinant Sema3A.Apoptotic cells were determined by a terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick end labeling method.RESULTS:In vitro,immunohistochemistry study revealed that Sema3A and Nrp-1 were constitutively expressed in hepatocytes and SECs,respectively,in normal rat liver tissues.Nrp-1 expression in SECs was quantified by the percentage of immunostained area with antiNrp-1 antibody in relation to the area stained with SE-1.Between 24 h and 96 h following resection of liver,Nrp-1 expression in SECs was transiently increased.Compared with the baseline(5.2% ± 0.1%),Nrp-1 expression in SECs significantly increased at 24 h(17.3% ± 0.7%,P < 0.05),48 h(39.1% ± 0.6%,P < 0.01),72 h(46.9% ± 4.5%,P < 0.01) and 96 h(29.9% ± 3.8%,P < 0.01) after PHx,then returned to the basal level at termination of liver regeneration.Interestingly,the expression of Sema3A was inversely associated with that of Nrp-1 in liver after PHx.Sema3A mRNA expression was significantly reduced by about 75% over the period 24-144 h after PHx(P < 0.05),and returned to basal levels at 192 h after PHx.In vitro,SECs isolated from rats after PHx(PHx-SECs) were observed to migrate to the lower chamber of the cell transwell system after incubation for 24 h,but not cells from normal rats(CONT-SECs),indicating that mobility of PHx-SECs increases as compared with that of CONT-SECs.Moreover,recombinant Sema3A significantly attenuated migration in PHx-SECs in primary culture(vehicle-treated 100% ± 7.9% vs Sema3A-treated 42.6% ± 5.4%,P < 0.01),but not in CONT-SECs.Compared with CONTSECs,the apoptotic rate of PHx-SECs decreased by 78.3%(P < 0.05).There was no difference in apoptosis between CONT-SECs that were treated with vehicle and Sema3A.However,in PHx-SECs,apoptosis was induced by the presence of 5 nmol Sema3A for 24 h(vehicle-treated 21.7% ± 7.6% vs Sema3A-treated 104.3% ± 8.9%,P < 0.05).In addition,immunohistochemistry confirmed the increased expression of Nrp-1 in PHx-SECs,while it was noted to a lesser extent in CONT-SECs.CONCLUSION:The interplay of Nrp-1 and Sema3A shown in our results may lead to a better understanding of interaction between sinusoidal remodeling and SECs during liver regeneration. | Ling Fu Tsuneo Kitamura Kazuhisa Iwabuchi Syozo Ichinose Mitsuaki Yanagida Hideoki Ogawa Sumio Watanabe Toshihide Maruyama Masafumi Suyama Kenji Takamori | 2012 | World Journal of Gastroenterology2012，18，36： | 5 |
| 2 | Sm-doped CsBr crystal as a new radio-photoluminescence (RPL) material显示文摘Radio-photoluminescence(RPL) is a phenomenon seen in luminescent materials in which the appearance of new photoluminescence(PL) emission is induced by an incident ionizing radiation such as X-rays; and the signal is stable even after the irradiation and during the PL measurement. Since the induced PL intensity is proportional to the irradiation dose, the RPL can be used in radiation measurements. The distinct advantage of RPL over the conventional thermally- or photo-stimulated luminescence(abbreviated as TSL or PSL) dosimeters is the stability of response signal. With an RPL detector, it allows us to readout the signal multiple time without signal fading. In this work, we discovered that CsB r:Sm showed an RPL phenomenon by X-ray irradiation, and we characterized this new material as an RPL detector. While the sample showed PL emissions mainly in the visible range, after an X-ray irradiation additional emissions could be observed in the red to near-infrared range around 650–850 nm and 900–1000 nm and longer. The RPL response was fairly stable overall, but very interestingly the 650–850 nm signal slightly increased while the 900–1000 nm decreased during PL readout. The dynamic range was confirmed over 1–10~4 mG y with linear response. | Go Okada Yutaka Fujimoto Hironori Tanaka Safa Kasap Takayuki Yanagida | 2016 | Journal of Rare Earths2016，34，8： | 2 |
| 3 | Long-term outcomes of combination of endoscopic submucosal dissection and laparoscopic lymph node dissection without gastrectomy for early gastric cancer patients who have a potential risk of lymph node metastasis显示文摘 | Nobutsugu Abe Hirohisa Takeuchi Atsuko Ohki Osamu Yanagida Tadahiko Masaki Toshiyuki Mori Masanori Sugiyama | 2011 | Gastrointestinal Endoscopy2011，，4： | 2 |
| 4 | Effectiveness of treating menorrhagia using microwave endometrial ablation at a frequency of 2.45 GHz显示文摘BACKGROUND Microwave endometrial ablation(MEA)is a minimally invasive treatment for menorrhagia.It has been covered by the national insurance in Japan since April 2012,and its demand has been increasing as the importance of women’s health has advanced in society.AIM To examine the efficacy of MEA as a treatment option for menorrhagia.METHODS In this study,we retrospectively analyzed 76 patients who underwent MEA between January 2016 and March 2020 in our department.MEA was performed in the lithotomy position,under general anesthesia,and with transabdominal ultrasound guidance,including the entire endometrial circumference while confirming endometrial coagulation.The Microtaze AFM-712 and the Sounding Applicator CSA-40CBL-1006200C were used for MEA,and the endometrium was ablated using a Microtaze output of 70 W and coagulation energization time of 50 s per cycle.The visual analog scale(VAS)was used to evaluate menorrhagia,menstrual pain,and treatment satisfaction.Additionally,the hemoglobin(Hb)levels before and after MEA and associated complications were investigated.RESULTS The average age of the patients was 44.8±4.0 years.While 14 patients had functional menorrhagia,62 had organic menorrhagia,of whom 14 had endometrial polyps,40 had uterine fibroids,and 8 had adenomyosis.The VAS score before MEA and 3 and 6 mo after the procedure were 10,1.3±1.3,and 1.3±1.3,respectively,for menorrhagia and 10,1.3±1.8,and 1.3±1.8,respectively,for menstrual pain,both showing improvements(P<0.001).The MEA Hb level significantly improved from 9.2±4.2 g/dL before MEA to 13.4±1.2 g/dL after MEA(P=0.003).Treatment satisfaction was high,with a VAS score of 9.6±0.7.Endometritis was observed in one patient after surgery and was treated with antibiotics.CONCLUSION MEA is a safe and effective treatment for menorrhagia. | Toshiyuki Kakinuma Ayaka Kaneko Kaoru Kakinuma Yoshio Matsuda Kaoru Yanagida NobuhiroTakeshima Michitaka Ohwada | 2023 | World Journal of Clinical Cases2023，11，24： | 2 |
| 5 | Toner prepared by the direct polymerization method in comparison with the pulverization method显示文摘 | HASEGAWA J YANAGIDA N MITSUHIRO T | 1999 | Colloids and Surfaeea A:Phyatochemical and Engineering Aspects1999，153，： | 1 |
| 6 | Effects of a fluoride etch- ant on resin bonding totitanium-aluminum-niobium alloy 显示文摘 | Yanagida H Taira Y Atsuta M | 2004 | EurJ Oral Sci2004，112，4： | 1 |
| 7 | Relations between two operator inequalities and their applications to paranormal operators显示文摘 | YAMAZAKI T YANAGIDA M | 2003 | Acta Sci Math(Szeged)2003，69，： | 1 |
| 8 | Nucleotide and predicted amino acid sequences of Marek's disease virus homologues of herpes simplex virus major tegument proteins 显示文摘 | Yanagida N Yoshida S Nazerian K | 1993 | J Gen Virol1993，74，9： | 1 |
| 9 | Neolignans and a phenylpropanoid glucoside from Illicium difengpi显示文摘 | Isao Kouno Yang Chunshun Yukari Yanagida | 1993 | Phytochemistry1993，32，11： | 1 |
| 10 | High-efficiency-sensitized solar cell with a novel co-adsorbent显示文摘 | Han L Y Islam A Chen H Malapaka C Chiranjeevi B Zhang S F Yang X D Yanagida M | | 0，，03： | 1 |
| 11 | Effect of Ce doping on scintillation characteristics of Li YF4single crystals forγ-ray detection显示文摘 | Fukabori A Yokota Y Yanagida T | 2011 | Nuclear Instruments and Methods in Physics Research Section A:Accelerators Spectrometers Detectors and Associated Equipment2011，631，1： | 1 |
| 12 | Fractionation of apple procyanidins according to their degree of polymerization by normal-phase high-performance liquid chromatography显示文摘 | YANAGIDA A KANDA T TAKAHASHI T | 2000 | J Chromatogr A2000，890，： | 1 |
| 13 | Phe- nylpropanoids from the barks oflllicium difengpi 显示文摘 | Isao Kouno Chun - shu Yang Yukari Yanagida | 1992 | Chem Pharm Bull1992，40，9： | 1 |
| 14 | Estimating economic impacts from tourism显示文摘 | ZHOU D Y YANAGIDA J F CHAKRAVORTY U LE-UNG P S | 1997 | Annals of Tourism Research1997，24，1： | 1 |
| 15 | Synthesis of potassium titanate fibers by kneading-drying-calcina-tion process 显示文摘 | Shimizu T Yanagida H Hashimoto K | 1978 | Yogyo-Kyokai-Shi1978，86，8： | 1 |
| 16 | A Novel Approach to Determine the Kinetics for Dynamic Recrystallization by Using the Flow Curve显示文摘 | Yanagida A Yanagimoto J | | 0，，1： | 1 |
| 17 | Positive Status of α-Fetoprotein and des-γ-Carboxy Prothrombin: Important Prognostic Factor for Recurrent Hepatocellular Carcinoma显示文摘 | Masaki Kaibori M.D. Yoichi Matsui M.D. Hidesuke Yanagida M.D. Norio Yokoigawa M.D. A-Hon Kwon M.D. Yasuo Kamiyama M.D | 2004 | World Journal of Surgery2004，，7： | 1 |
| 18 | Determining falling patterns by estimation of horizontal distance and height显示文摘 | Yanagida Y Maeda M Nushida H | 2011 | Int J Legal Med2011，125，1： | 1 |
| 19 | Role of income diversification in protecting natural forests:evidence from rural households in forest margins of Sri Lanka显示文摘 | Prabodh Illukpitiya John F Yanagida | 2008 | Agroforest Syst2008，74，： | 1 |
| 20 | Crystal growth and luminescence properties of Pr-doped Lu Li F4single crystal显示文摘 | Sugiyama M Yanagida T Yokota Y | 2013 | Radiation Measurements2013，55，： | 1 |
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