|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Key residues of the receptor binding motif in the spike protein of SARS-CoV-2 that interact with ACE2 and neutralizing antibodies显示文摘Coronavirus disease 2019(COVID-19),caused by the novel human coronavirus SARS-CoV-2,is currently a major threat to public health worldwide.The viral spike protein binds the host receptor angiotensin-converting enzyme 2(ACE2)via the receptor-binding domain(RBD),and thus is believed to be a major target to block viral entry.Both SARS-CoV-2 and SARS-CoV share this mechanism.Here we functionally analyzed the key amino acid residues located within receptor binding motif of RBD that may interact with human ACE2 and available neutralizing antibodies.The in vivo experiments showed that immunization with either the SARS-CoV RBD or SARS-CoV-2 RBD was able to induce strong clade-specific neutralizing antibodies in mice;however,the cross-neutralizing activity was much weaker,indicating that there are distinct antigenic features in the RBDs of the two viruses.This finding was confirmed with the available neutralizing monoclonal antibodies against SARS-CoV or SARS-CoV-2.It is worth noting that a newly developed SARS-CoV-2 human antibody,HA001,was able to neutralize SARS-CoV-2,but failed to recognize SARS-CoV.Moreover,the potential epitope residues of HA001 were identified as A475 and F486 in the SARS-CoV-2 RBD,representing new binding sites for neutralizing antibodies.Overall,our study has revealed the presence of different key epitopes between SARS-CoV and SARSCoV-2,which indicates the necessity to develop new prophylactic vaccine and antibody drugs for specific control of the COVID-19 pandemic although the available agents obtained from the SARS-CoV study are unneglectable. | Chunyan Yi Xiaoyu Sun Jing Ye Longfei Ding Meiqin Liu Zhuo Yang Xiao Lu Yaguang Zhang Liyang Ma Wangpeng Gu Aidong Qu Jianqing Xu Zhengli Shi Zhiyang Ling Bing Sun | 2020 | Cellular & Molecular Immunology2020，17，6： | 13 |
| 2 | Presence of carboxylate salts in marine carbonate strata of the Ordos Basin and their impact on hydrocarbon generation evaluation of low TOC, high maturity source rocks显示文摘The total organic carbon(TOC)in the marine source rock of the Ordos Basin mostly ranges from 0.2%to 0.5%.The industrial standard commonly states that the TOC value has to be no less than 0.5%(0.4%for high mature or over-mature source rock)to form large petroleum reservoirs.However,gas source correlation indicates that the natural gas in the Jingbian gas field does receive contribution from marine source rocks.In order to determine the effect of carboxylate salts(or called as organic acid salts)on TOC in highly mature source rocks with low TOC value,we sampled the Ordovician marine source rock and the Permian transitional facies source rock in one drilled well in the southern Ordos Basin and performed infrared and GC-MS analysis.It is found that both kerogen-derived organic acids and carboxylate salt-conversed organic acids exist in both marine and transitional facies source rocks.The carboxylate salt-conversed organic acids mainly come from the complete acidification of carboxylate salts,which confirms the presence of carboxylate salts in the marine source rocks.Although the C16:O peak is the main peak for the organic acids both before and after acidification,the carboxylate salt-conversed organic acids have much less relative abundance ahead of C16:O compared with that of the kerogen-based and free organic acids.This observation suggests that the kerogen-based and free organic acids mainly decarboxylate to form lower carboxylic acids,whereas the carboxylate salt-conversed organic acids mainly break down into paraffins.By using calcium hexadecanoate as the reference to quantify the kerogen-derived and carboxylate salt-conversed organic acids,the high TOC(>2.0%)marine source rocks have low carboxylate salt content and the low TOC(0.2%–0.5%)marine source rocks contain high content of carboxylate salt.Therefore,for the marine source rocks with 0.2%–0.5%TOC,the carboxylate salts may be a potential gas source at high maturity stage. | LIU QuanYou JIN ZhiJun LIU WenHui LU LongFei MENG QianXiang TAO Ye HAN PinLong | 2013 | Science China Earth Sciences2013，56，12： | 9 |
| 3 | A comparison of pilot scale electron beam and bench scale gamma irradiations of cyanide aqueous in solution显示文摘 | YE Longfei HE Shijun YANG Chunping WANG Jianlong YU Jiang | 2013 | Nuclear Science and Techniques2013，24，B12： | 4 |
| 4 | Continuous synthesis of iron oxide (Fe3O4) nanoparticles via thermal decomposition显示文摘 | William Glasgow Ben Fellows Bin Qi Taghi Darroudi Christopher Kitchens Longfei Ye Thomas M. Crawford O. Thompson Mefford | 2016 | Particuology2016，14，3： | 2 |
| 5 | Formation, preservation and connectivity control of organic pores in shale显示文摘In view of strong heterogeneity and complex formation and evolution of organic pores,field emission scanning electron microscopy(FESEM),Raman spectrum and fluid injection+CT/SEM imaging technology were used to study the macerals,organic pores and connectivity of organic pores in the lower Paleozoic organic-rich shale samples from Southern China.Combined with the mechanism of hydrocarbon generation and expulsion and pore forming mechanism of organic matter-based activated carbon,the relationships between organic pore development and the organic matter type,hydrocarbon generation process,diagenesis and pore pressure were explored to reveal the controlling factors of the formation,preservation and connectivity of organic pores in shale.(1)The generation of organic pores goes on through the whole hydrocarbon generation process,and is controlled by the type,maturity and decomposition of organic matter;the different hydrocarbon generation components and differential hydrocarbon-generation evolution of kerogen and solid asphalt lead to different pore development characteristics;organic pores mainly develop in solid bitumen and hydrogen-rich kerogen.(2)The preservation of organic pores is controlled by maturity and diagenesis,including the steric hindrance effect of in-situ hydrocarbon retention,rigid mineral framework formed by recrystallization,the coupling mechanism of pore-fluid pressure and shale brittleness-ductility transition.(3)The Ro of 4.0%is the maturity threshold of organic pore extinction,the shale layers with Ro larger than 3.5%have high risk for shale gas exploration,these shale layers have low gas contents,as they were in an open state before uplift,and had high hydrocarbon expulsion efficiency and strong aromatization,thus having the'congenital deficiency'of high maturity and pore densification.(4)The pores in the same organic matter particle have good connectivity;and the effective connectivity between different organic matter pores and inorganic pores and fractures depends on the abundance and distribution of organic matter,and development degree of pores and fractures in the shale;the accumulation,preservation and laminar distribution of different types of organic matter in high abundance is the prerequisite for the development and connection of organic pores,grain margin fractures and bedding fractures in reservoir. | BORJIGIN Tenger LU Longfei YU Lingjie ZHANG Wentao PAN Anyang SHEN Baojian WANG Ye YANG Yunfeng GAO Zhiwei | 2021 | Petroleum Exploration and Development2021，48，4： | 1 |
| 6 | Sol-gel approach to low-temperature synthesis of single-phase metastable La_(2)Ga_(3)O_(7.5) melilite with enhanced grain-boundary oxide ionic conductivity via a kinetically favorable mechanism显示文摘Starting with the stoichiometric and highly homogeneous gel-precursor,single-phase metastable melilite La_(2)Ga_(3)O_(7.5),as the end-member of solid solution La_(1+x)Sr_(1−x)Ga_(3)O_(7+x/2)(0≤x≤1),has been synthesized by solid-state reaction at 700℃ for 2 h via a kinetically favorable mechanism and characterized by X-ray diffraction(XRD),Raman,X-ray photoelectron spectroscopy(XPS),field emission scanning electron microscopy(FESEM),transmission electron microscopy(TEM),AC impedance spectroscopy,etc.It has been revealed that the as-synthesized melilite La_(2)Ga_(3)O_(7.5) shows an orthorhombic symmetry with crystal cell parameters a=11.4690(1)A,b=11.2825(4)A,and c=10.3735(4)A,while has more Raman active modes than LaSrGa_(3)O_(7) with a tetragonal structure,which was also synthesized under the same conditions for comparison,but tends to slowly decompose into perovskite LaGaO_(3) and Ga2O_(3) when annealed at 700℃ for over 20 h driven by its meta-stability.Moreover,the metastable La2Ga3O7.5 shows a higher XPS binding energy for the excess oxide ions in the crystal structure than those at normal lattice sites.Its intrinsic grain oxide ion conductivity can reach as high as 0.04 and 0.51 mS·cm^(-1) at 550 and 700℃,respectively,characterized by a simple Arrhenius relationship ln(σT)-1/T with invariable activation energy,E_(a)=1.22 eV,over the temperature range from 300 to 700℃,along with an apparent grain boundary conductivity that is about double that from the grains thanks to the clean grain boundaries.This paper provides a new strategic approach to the synthesis of complex oxides that may be of high performance but are difficultly achieved by the conventional ceramic method at high temperatures. | Yuan ZHANG Longfei ZHAO Zhupeng YE Yanwei ZENG | 2022 | Journal of Advanced Ceramics2022，11，10： | 0 |
| 7 | iVenomDB: A manually curated database for insect venom proteins显示文摘DearEditor,Venomous animals,such as snakes,insects(wasps,bees,ants,and some bugs),spiders,scorpions,and cone snails,use venom for predation and/or defense.Venom is mostly composed of proteinsand peptides.Because of their high potency and selectivity,venom components have been developed into valuable resources,such as research tools,bioinsecticides(Heep et al.,2019),and drug leads(Badawi,2021).The advent of high-throughput sequencing and mass spectrometry enabled exploration of venom components to new depths and at an unprecedented pace.To be useful to the scientific community,knowledge on these proteins must be stored in a retrievable manner,annotated,and curated.Currently,several databases are dedicated to venoms from a wide range of animals,such as Arachnoserver(Pineda et al.,2018),Conoserver(Kaas et al.,2012),and the Tox-Prot program within UniprotKB(Jungo et al.,2012). | Longfei Chen Kun Lang Bo Zhang Jiamin Shi Xinhai Ye David W.Stanley Qi Fang Gongyin Ye | 2023 | Insect Science2023，30，1： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员