|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Aliskiren ameliorates pressure overload-induced heart hypertrophy and fibrosis in mice显示文摘 | Li-qing WENG Wen-bin ZHANG Yong YE Pei-pei YIN Jie YUAN Xing-xu WANG Le KANG Sha-sha JIANG Jie-yun YOU Jian WU Hui GONG Jun-bo GE Yun-zeng ZOU | 2014 | Acta Pharmacologica Sinica2014，35，8： | 12 |
| 2 | A human circulating immune cell landscape in aging and COVID-19显示文摘Age-associated changes in immune cells have been linked to an increased risk for infection.However,a global and detailed characterization of the changes that human circulating immune cells undergo with age is lacking.Here,we combined scRNA-seq,mass cytometry and sCATAC-seq to compare immune cell types in peripheral blood collected from young and old subjects and patients with COVID-19.We found that the immune cell landscape was reprogrammed with age and was characterized by T cell polarization from naive and memory cells to effector,cytotoxic,exhausted and reg-ulatory cells,along with increased late natural killer cells,age-associated B cells,inflammatory monocytes and age-associated dendritic cells.In addition,the expression of genes,which were implicated in coron-avirus susceptibility,was upregulated in a cell subtype-specific manner with age.Notably,COVID-19 promoted age-induced immune cell polarization and gene expression related to inflammation and cellular senes-cence.Therefore,these findings suggest that a dysreg-ulated immune system and increased gene expression associated with SARS-CoV-2 susceptibility may at least partially account for COVID-19 vulnerability in the elderly. | Yingfeng Zheng Xiuxing Liu Wenqing Le Lihui Xie He Li Wen Wen Si Wang Shuai Ma Zhaohao Huang Jinguo Ye Wen Shi Yanxia Ye Zunpeng Liu Moshi Song Weiqi Zhang Jing-Dong J.Han Juan Carlos lzpisua Belmonte Chuanle Xiao Jing Qu Hongyang Wang Guang-Hui Liu Wenru Su | 2020 | Protein & Cell2020，11，10： | 10 |
| 3 | A New Arg54Gly Transthyretin Gene Mutation Associated with Vitreous Amyloidosis in Chinese显示文摘Purpose:To analyse the hereditary features of a Chinese pedigree with familial vitreous amyloidosis in Liaoning Province,China,and to investigate the correlation between the clinical appearance of the disease and transthyretin(TTR)gene mutation,including the locus and type of TTR gene mutation.Methods:Five patients (10 eyes) from one Chinese family were diagnosed with vitreous amyloidosis between July 1996 and April 2009.Family members were followed up subsequently,and peripheral venous blood was obtained from 13 subjects (including 2 patients,and 11 controls without clinical signs of disease).DNA samples were extracted and 4 exons of the TTR gene were amplified by polymerase chain reaction (PCR).The gene fragments were subjected to sequencing analysis.The results were analyzed with DNAMAN Windows 5.2.2.0 and Chromas sequence chart analysis software,TTR gene exons were compared between affected patients and normal controls.Results:Family pedigree analysis revealed that patients were distributed in three generations.Male and female subjects had equal prevalence,and only one parent of affected patients had signs of disease.TTR gene exon sequencing showed that the sequence of patients was identical to that of normal individuals.No TTR gene mutations were noted in 10 unaffected family members.However,a TTR Gly-54 point mutation in the 2nd exon was detected in two patients and 1 unaffected family member (one of the patients' daughters).Vitreous samples in 4 cases (7 eyes) showed positive Congo red staining,suggesting that these family members suffered from familial vitreous amyloidosis.Conclusion:This pedigree affected with familial vitreous amyloidosis was characterized by autosomal dominant inheritance;.a TTR Gly-54 point mutation in the 2nd exon is presumed to be the cause.This Gly-54 point mutation of the TTR gene is a novel mutation in vitreous amyloidosis. | Yining Shi Jing Li Jia Hu Jun Hu Lijun Sun Huijin Li Rui Shi Le Yang Ye Sun Chan Li | 2011 | Eye Science2011，26，4： | 9 |
| 4 | A Comprehensive Power Flow Approach for Multi-terminal VSC-HVDC System Considering Cross-regional Primary Frequency Responses显示文摘For the planning,operation and control of multiterminal voltage source converter(VSC)based high-voltage direct current(HVDC)(VSC-MTDC)systems,an accurate power flow formulation is a key starting point.Conventional power flow formulations assume the constant frequencies for all asynchronous AC systems.Therefore,a new feature about the complex coupling relations between AC frequencies,DC voltages and the exchanged power via VSC stations cannot be characterized if VSC-MTDC systems are required to provide cross-regional frequency responses.To address this issue,this paper proposes a comprehensive frequency-dependent power flow formulation.The proposed approach takes the frequencies of asynchronous AC systems as explicit variables,and investigates the novel bus models of the interlinking buses of VSC stations.The proposed approach accommodates different operation modes and frequency droop strategies of VSC stations,and considers the power losses of VSC stations.The effectiveness and generality of the developed approach are validated by a 6-terminal VSC-HVDC test system.The test system presents the characteristics of the coexistence of numerous VSC operation modes,the absence of slack buses in both AC and DC subsystems,and diversified grid configurations such as point-to-point integration of renewable energy sources and one AC system integrated with multiple VSC stations. | Yida Ye Ying Qiao Le Xie Zongxiang Lu | 2020 | Journal of Modern Power Systems and Clean Energy2020，8，2： | 9 |
| 5 | The value of color Doppler ultrasonography in monitoring normal orthotopic liver transplantation and postoperative complications显示文摘OBJECTIVE: To assess the value of color Doppler ultrasonography in monitoring normal orthotopic livertransplantation and postoperative complications.METHODS: Forty-one patients after orthotopic liver transplantation were examined by using colorDoppler flow imaging to observe the hepatic blood flow and change of ultrasonography of the hepaticparenchyma and bile duct. The measured indexes included maximum blood flow velocity, time-averageblood flow velocity (TAV), resistance index (RI) and diameter of the bile duct.RESULTS: Among 41 patients, 17 (41.5%) suffered from liver transplant rejection. Of the 17patients, 13 (76.4%) showed decrease of TAV of the portal vein, 15 (88.25%) low-amplitudesingle-phase serrated wave or negative biphasic wave of the hepatic vein, 9 (52.9%) increased hepaticarterial RI, and 5 (29.4%) slightly dilated bile duct. Sonography showed disappearance of the hepaticartery blood flow around the portal vein in 5 (12.2%) of the 41 patients with hepatic artery thrombosisin the postoperative period. Slight dilatation of the intrahepatic bile duct was found in 3 (7.3%) of the41 patients in the early postoperational period and it normalized within 2 weeks. Ultrasonography of20 patients (48.8%) revealed a visible dilatation of the intrahepatic bile duct, which was worseninggradually. The causes of bile duct dilatation included biliary stricture in 2 patients (10%), stone in 15patients (75%) and others in 3 patients (15%).CONCLUSIONS: Color Doppler ultrasonography is valuable for monitoring normal liver transplantationand postoperative complications. | Dao-Zhong Huang Gui-Rong Le Qing-Ping Zhang Kai-Yan Li Qi-Fa Ye Wei Zhu Yun-Chao Chen the Department of Ultrasound, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China | 2003 | Hepatobiliary & Pancreatic Diseases International2003，2，1： | 7 |
| 6 | Oxaliplatin and 5-fluorouracil hepatic infusion with lipiodolized chemoembolization in large hepatocellular carcinoma显示文摘AIM: To investigate transarterial chemoembolization(TACE) with hepatic infusion of oxaliplatin and 5-fluorouracil and Lipiodol chemoembolization in large hepatocellular carcinoma(HCC).METHODS: In this retrospective study, 132 patients with unresectable HCCs larger than 10 cm were treated with hepatic infusion of oxaliplatin and 5-fluorouracil followed by Lipiodol chemoembolization. The primary endpoint was overall survival(OS). Sixteen-week disease-control rate, time to progression(TTP), and major complications were also studied. Univariate and multivariate analyses were performed to identify prognostic factors affecting OS and TTP.RESULTS: A total of 319 procedures were performed in the 132 patients. Eleven(8.3%) patients received radical resection following TACE treatment(median time to initial TACE 4.3 ± 2.3 mo). The median OS and TTP were 10.3 and 3.0 mo respectively, with a 50.0% 16-wk disease-control rate. Major complications were encountered in 6.0%(8/132) of patients following TACE and included serious jaundice in 1.5%(2/132) patients, aleukia in 1.5%(2/132), and hepatic failure in 3.0%(4/132). One patient died within one month due to serious hepatic failure and severe sepsis after receiving the second TACE. The risk factor associated with TTP was baseline alpha-fetoprotein level, and vascular invasion was an independent factor related to OS.CONCLUSION: Hepatic infusion of oxaliplatin and 5-fluorouracil followed by lipiodolized-chemoembolization is a safe and promising treatment for patients with HCCs larger than 10 cm in diameter. | Jing-Huan Li Xiao-Ying Xie Lan Zhang Fan Le Ning-Ling Ge Li-Xin Li Yu-Hong Gan Yi Chen Ju-Bo Zhang Tong-Chun Xue Rong-Xin Chen Jing-Lin Xia Bo-Heng Zhang Sheng-Long Ye Yan-Hong Wang Zheng-Gang Ren | 2015 | World Journal of Gastroenterology2015，21，13： | 6 |
| 7 | Failure probability estimation of overhead transmission lines considering spatial and temporal variation in severe weather显示文摘Estimation for weather-related failure probability of overhead transmission lines is essential in the reliability assessment of a power system.This paper analyzes the outage and weather data of 110 kV overhead transmission lines in the Guangxi Zhuang Autonomous Region of China during 2011–2014.The result reveals obvious uneven distributions of outage events for time and space due to the spatial and temporal variation of severe weather.Based on the results, an estimation method is proposed in this paper.Split and aggregation is used to smooth the outage and weather data.The poisson model is adopted in our method to investigate the statistic characteristics of transmission line outage events.Regression analysis is applied to obtain the correlation between the weather intensity and history failure rate.Furthermore the method proposed is validated against the empirical outage data. | Shuai YANG Wenjun ZHOU Shiyang ZHU Le WANG Lisha YE Xuezhi XIA Han LI | 2019 | Journal of Modern Power Systems and Clean Energy2019，7，1： | 5 |
| 8 | Score model for predicting acute-on-chronic liver failure risk in chronic hepatitis B显示文摘AIM: To establish a clinical scoring model to predict risk of acute-on-chronic liver failure(ACLF) in chronic hepatitis B(CHB) patients.METHODS: This was a retrospective study of 1457 patients hospitalized for CHB between October 2008 and October 2013 at the Beijing Ditan Hospital, Capital Medical University, China. The patients were divided into two groups: severe acute exacerbation(SAE) group(n = 382) and non-SAE group(n = 1075). The SAE group was classified as the high-risk group based on the higher incidence of ACLF in this group than in the non-SAE group(13.6% vs 0.4%). Two-thirds of SAE patients were randomly assigned to risk-model derivation and the other one-third to model validation. Univariate risk factors associated with the outcome were entered into a multivariate logistic regression model for screening independent risk factors. Each variable was assigned an integer value based on the regression coefficients, and the final score was the sum of these values in the derivation set. Model discrimination and calibration were assessed using area under the receiver operating characteristic curve and the Hosmer-Lemeshow test. RESULTS: The risk prediction scoring model includedthe following four factors: age ≥ 40 years, total bilirubin ≥ 171 μmol/L, prothrombin activity 40%-60%, and hepatitis B virus DNA > 107 copies/m L. The sum risk score ranged from 0 to 7; 0-3 identified patients with lower risk of ACLF, whereas 4-7 identified patients with higher risk. The Kaplan-Meier analysis showed the cumulative risk for ACLF and ACLF-related death in the two risk groups(0-3 and 4-7 scores) of the primary cohort over 56 d, and log-rank test revealed a significant difference(2.0% vs 33.8% and 0.8% vs 9.4%, respectively; both P < 0.0001). In the derivation and validation data sets, the model had good discrimination(C index = 0.857, 95% confidence interval: 0.800-0.913 and C index = 0.889, 95% confidence interval: 0.820-0.957, respectively) and calibration demonstrated by the Hosmer-Lemeshow test(χ2 = 4.516, P = 0.808 and χ2 = 1.959, P = 0.923, respectively).CONCLUSION: Using the scoring model, clinicians can easily identify patients(total score ≥ 4) at high risk of ACLF and ACLF-related death early during SAE. | Fang-Yuan Gao Yao Liu Xiao-Shu Li Xie-Qiong Ye Le Sun Ming-Fan Geng Rui Wang Hui-Min Liu Xiao-Bing Zhou Li-Li Gu Yan-Min Liu Gang Wan Xian-Bo Wang | 2015 | World Journal of Gastroenterology2015，21，27： | 5 |
| 9 | Upregulation of miR-489-3p and miR-630 inhibits oxaliplatin uptake in renal cell carcinoma by targeting OCT2显示文摘Renal cell carcinoma(RCC) is one of the most common malignant tumors affecting the urogenital system, accounting for 90% of renal malignancies. Traditional chemotherapy options are often the front-line choice of regimen in the treatment of patients with RCC, but responses may be modest or limited due to resistance of the tumor to anticarcinogen. Downregulated expression of organic cation transporter OCT2 is a possible mechanism underlying oxaliplatin resistance in RCC treatment. In this study, we observed that mi R-489-3 p and mi R-630 suppress OCT2 expression by directly binding to the OCT2 30-UTR. Meanwhile, via 786-O-OCT2-mi RNAs stable expression cell models, we found that mi RNAs could repress the classic substrate 1-methyl-4-phenylpyridinium(MPP+), fluorogenic substrate N,N-dimethyl-4-(2-pyridin-4-ylethenyl) aniline(ASP+), and oxaliplatin uptake by OCT2 both in vitro and in xenografts. In 33 clinical samples, mi R-489-3 p and mi R-630 were significantly upregulated in RCC, negatively correlating with the OCT2 expression level compared to that in adjacent normal tissues, using tissue microarray analysis and q PCR validation. The increased binding of c-Myc to the promoter of pri-mi R-630, responsible for the upregulation of mi R-630 in RCC, was further evidenced by chromatin immunoprecipitation and dual-luciferase reporter assay. Overall, this study indicated that mi R-489-3 p and mi R-630 function as oncotherapy-obstructing micro RNAs by directly targeting OCT2 in RCC. | Lu Chen Le Chen Zhiyuan Qin Jinxiu Lei Sheng Ye Kui Zeng Hua Wang Meidan Ying Jianqing Gao Su Zeng Lushan Yu | 2019 | Acta Pharmaceutica Sinica B2019，9，5： | 5 |
| 10 | Face Masks in the New COVID-19 Normal: Materials, Testing,and Perspectives显示文摘The increasing prevalence of infectious diseases in recent decades has posed a serious threat to public health.Routes of transmission differ,but the respiratory droplet or airborne route has the greatest potential to disrupt social intercourse,while being amenable to prevention by the humble facemask.Different types of masks give different levels of protection to the user.The ongoing COVID-19 pandemic has even resulted in a global shortage of face masks and the raw materials that go into them,driving individuals to self-produce masks from household items.At the same time,research has been accelerated towards improving the quality and performance of face masks,e.g.,by introducing properties such as antimicrobial activity and superhydrophobicity.This review will cover mask-wearing from the public health perspective,the technical details of commercial and home-made masks,and recent advances in mask engineering,disinfection,and materials and discuss the sustainability of mask-wearing and mask production into the future. | Ming Hui Chua Weiren Cheng Shermin Simin Goh Junhua Kong Bing Li Jason Y.CLim Lu Mao Suxi Wang Kun Xue Le Yang Enyi Ye Kangyi Zhang Wun Chet Davy Cheong Beng Hoon Tan Zibiao Li Ban Hock Tan Xian Jun Loh | 2020 | Research2020，，1： | 5 |
| 11 | Regulation of microbiota–GLP1 axis by sennoside A in diet-induced obese mice显示文摘Sennoside A(SA) is a bioactive component of Chinese herbal medicines with an activity of irritant laxative, which is often used in the treatment of constipation and obesity. However, its activity remains unknown in the regulation of insulin sensitivity. In this study, the impact of SA on insulin sensitivity was tested in high fat diet(HFD)-induced obese mice through dietary supplementation. At a dosage of 30 mg/kg/day, SA improved insulin sensitivity in the mice after 8-week treatment as indicated by HOMA-IR(homeostatic model assessment for insulin resistance) and glucose tolerance test(GTT). SA restored plasma level of glucagon-like peptide 1(GLP1) by 90% and mRNA expression of Glp1 by 80% in the large intestine of HFD mice. In the mechanism, SA restored the gut microbiota profile, short chain fatty acids(SCFAs), and mucosal structure in the colon. A mitochondrial stress was observed in the enterocytes of HFD mice with ATP elevation, structural damage, and complex dysfunction. The mitochondrial response was induced in enterocytes by the dietary fat as the same responses were induced by palmitic acid in the cell culture. The mitochondrial response was inhibited in HFD mice by SA treatment. These data suggest that SA may restore the function of microbiota–GLP1 axis to improve glucose metabolism in the obese mice. | Jiamei Le Xiaoying Zhang Weiping Jia Yong Zhang Juntao Luo Yongning Sun Jianping Ye | 2019 | Acta Pharmaceutica Sinica B2019，9，4： | 5 |
| 12 | Heritability of growth traits in the Asian seabass (Lates calcarifer)显示文摘Growth is an economically important trait in aquaculture.To improve growth trait of the Asian seabass(Lates calcarifer)we have been carrying out,since 2004,a selective breeding program.This study focuses on growth traits in the F2 fish generation,comprised of offspring from 23 mass crosses from 383 F_(1) brooders.Using genotyping analysis for 10 microsatellites from both brood stock and progeny we have reconstructed the pedigree of each mass-cross.For F_(2) generation at 90 days post hatch(dph),we have recorded body weight(BW)for 12,117 individuals and total length(TL),standard length(SL)and condition factor(K_(tl) and K_(sl))for 3530 individuals;and all five traits for 2136 individuals at 270 dph.At 90 dph the average BW was 46.88±20.95 g.Combining pedigree information,recorded growth traits and Restricted Maximum Likelihood method,we have estimated that the narrow sense heritability(h^(2))in F_(1) fish for BW,TL,SL,K_(tl) and K_(sl) was at,90 dph,0.12±0.03,0.11±0.03,0.10±0.03,0.20±0.04 and 0.11±0.03,respectively and,at 270 dph,0.34±0.07,0.32±0.07,0.30±0.06,0.13±0.04 and 0.11±0.04,respectively.At 90 dph the realised heritability for BW was 0.13.Comparing with F_(1) generation,the growth performance of F_(2) fish was increased by 14.4%.Heritability of growth traits will be useful for future genetic improvement programmes of the Asian seabass. | Baoqing Ye Ziyi Wan Le Wang Hongyan Pang Yanfei Wen Huiming Liu Bing Liang Huan Sein Lim Junhui Jiang Genhua Yue | 2017 | Aquaculture and Fisheries2017，2，3： | 4 |
| 13 | Experimental research of air-throttling ignition for a scramjet at Ma 6.5显示文摘An experimental investigation on ignition characteristics with air-throttling in an ethylene-fueled scramjet under flight Ma 6.5 conditions was conducted.The dynamic process of air-throttling ignition was explored systematically.The influences of throttling parameters,i.e.,throttling mass rate and duration,were investigated.When the throttling mass rate was 45% of the inflow mass rate,ambient ethylene could be ignited reliably.The delay time from ignition to throttling was about 45–55 ms.There was a threshold of throttling duration under a certain throttling mass rate.It was shorter than 100 ms when the throttling mass rate was 45%.While a 45%throttling mass rate would make the shock train propagate upstream to the isolator entry in about10–15 ms,four lower throttling mass rates were tested,including 30%,25%,20%,and 10%.All of these throttling mass rates could ignite ethylene.However,combustion performances varied with them.A higher throttling mass rate made more ethylene combust and produced higher wall pressure.Through these experiments,some aspects of the relationships between ignition,flame stabilization,combustion efficiency,and air-throttling parameters were brought to light.These results could also be a benchmark for CFD validation. | Weixin DENG Jialing LE Shunhua YANG Wanzhou ZHANG Ye TIAN | 2017 | Chinese Journal of Aeronautics2017，30，3： | 4 |
| 14 | Individualized minimally invasive treatment for adult testicular hydrocele: A pilot study显示文摘BACKGROUND Hydrocelectomy is the gold standard for the treatment of hydrocele,but it often causes complications after surgery,including hematoma,infection,persistent swelling,hydrocele recurrence,and chronic pain. In recent years,several methods for minimally invasive treatment of hydrocele have been introduced,but they all have limitations. Herein,we introduce a new method of individualized minimally invasive treatment for hydrocele.AIM To present a new method for the treatment of adult testicular hydrocele.METHODS Fifty-two adult patients with idiopathic testicular hydrocele were included. The key point of this procedure was that the scope of the resection of the sheath of the tunica vaginalis was determined according to the maximum diameter(d) of the effusion measured by ultrasound and the maximum diameter of the portion of the sheath pulled out of the scrotum was approximately πd/2. The surgical procedure consisted of a 2-cm incision in the anterior wall of the scrotum,drainage of the effusion,and dissection of part of the sheath of the tunica vaginalis. After the sheath was peeled away to the predetermined target extent,the pulled-out sheath was removed. The intraoperative findings and postoperative complications were analyzed.RESULTS All patients were successfully treated with a median operation time of 18 min.The median maximum diameter of the effusion on ultrasound was 3.5 cm,and the median maximum diameter of the resected sheath was 5.5 cm. Complications occurred in four(7.7%) patients: two(3.8%) cases of mild scrotal edema,one(1.9%) case of scrotal hematoma,and one(1.9%) case of wound infection. All of the complications were grade I-II. Recurrent hydrocele,chronic scrotal pain,and testicular atrophy were not observed during a median follow-up of 12 mo.CONCLUSION We report a new technique for individualized treatment of testicular hydrocele,which is quantitative and minimally invasive and yields good outcomes. Further study is warranted to verify its potential value in clinical practice. | Le Lin Huai-Shan Hong Yun-Liang Gao Jin-Rui Yang Tao Li Qing-Guo Zhu Lie-Fu Ye Yong-Bao Wei | 2019 | World Journal of Clinical Cases2019，7，6： | 4 |
| 15 | p53 isoform △113p53/△133p53 promotes DNA double-strand break repair to protect cell from death and senescence in response to DNA damage显示文摘在 DNA 双海滨的 p53 的禁止的角色裂缝(DSB ) 修理对它的压制肿瘤的性质似乎矛盾。p53 isoform Δ 113p53/Δ 133p53 是反对 p53 apoptotic 的 p53 目标基因活动。然而,它在 DNA 损坏修理的功能的信息正在缺乏。这里,我们报导那 Δ 113p53 表示被 γ 强烈导致; -irradiation, 然而并非由紫外照耀或热电震疗法。惊人地, Δ 113p53 支持 DNA DSB 修理小径包括相应再结合,非相应的结束加入并且单个海滨的退火。学习 Δ 的生物意义;在支持 DNA DSB 修理的 113p53,我们产生了 zebrafish Δ 113p53 经由抄写的 M/M 异种像使活跃之物的受动器核酸酶技术并且发现异种对 γ 更敏感;照耀。人的 ortholog, Δ 133p53,被 γ 仅仅也导致;照耀和功能修理支持 DNA DSB。Δ 133p53-knockdown 房间响应 γ 在以后的阶段在 G2 阶段被逮捕;照耀由于 unrepaired DNA DSB 的高水平,它最后导致了房间老朽。而且, Δ 113p53/Δ 133p53 经由 upregulating 支持 DNA DSB 修理由到在他们的倡导者的 p53 应答的元素的一种新奇类型的绑定的修理基因 rad51, lig4 和 rad52 的抄写。我们的结果表明那 Δ 113p53/Δ 133p53 是为由阻止 apoptosis 并且支持 DNA DSB 修理禁止房间老朽的 DNA 损坏应力的一个进化地保存的支持幸存的因素。我们的数据也建议 Δ 的正式就职;在正常房间或纸巾的 133p53 表示为癌症病人提供一个重要忍耐标记给放射疗法。 | Lu Gong Hongjian Gong Xiao Pan Changqing Chang Zhao Ou Shengfan Ye Le Yin Lina Yang Ting Tao Zhenhai Zhang Cong Liu David P Lane Jinrong Peng Jun Chen | 2015 | Cell Research2015，25，3： | 4 |
| 16 | Estimating health damage cost from secondary sulfate particles—a case study of Hunan Province,China显示文摘China’s coal dominated energy pattern has resulted in large amount of SO 2 emissions. Estimate of the sulfur related health damage cost is necessary to help perform systematic cost benefit analysis and set national energy and emissions control priorities. Current researches were confined to gaseous SO 2 in urban areas; however, secondary sulfate (SO 2- 4) particles can exert serious impact in a wider region. Based on the concept of “intake fraction', CALPUFF long range dispersion model and 180 sample emission sources, multiple regression equation was obtained with good correlation( r =0 85), which illustrates that populations were key parameters to determine intake fraction but source characteristics were insignificant. Based on the formula and the population distribution data, county level intake fractions were mapped for Hunan Province(range: 1 1×10 -6 -3 2×10 -6 ) of China. A combination of county level SO 2 emissions with the intake fractions yields a total 1 98 tons of sulfate(SO 2- 4) inhalation, and resulting total health damage cost to be 0 76(willingness to pay approach, WTP) or 0 16(human capital approach, HC) billion USD in 1997, about 2.1% or 0 45% of GDP in Hunan in 1997. Average health damage cost per ton of SO 2 emission is 930(WTP) or 200 USD(HC). The results demonstrated that more stringent regulation should be forced. | HAO Ji ming, LI Ji, YE Xue mei, ZHU Tian le (Department of Environmental Science and Engineering, Tsinghua University, Beijing 100084, China. ) | 2003 | Journal of Environmental Sciences2003，15，5： | 3 |
| 17 | Validation of the DNATyper^(TM)15 PCR Genotyping System for Forensic Application显示文摘We describe the optimization and validation of the DNATyper^(TM)15 multiplex polymerase chain reaction(PCR)genotyping system for autosomal short tandem repeat(STR)amplification at 14 autosomal loci(D6S1043,D21S11,D7S820,CSF1PO,D2S1338,D3S1358,D13S317,D8S1179,D16S539,Penta E,D5S818,vWA,D18S51,and FGA)and amelogenin,a sex‑determining locus.Several DNATyper^(TM)15 assay variables were optimized,including hot start Taq polymerase concentration,Taq polymerase activation time,magnesium concentration,primer concentration,annealing temperature,reaction volume,and cycle number.The performance of the assay was validated with respect to species specificity,sensitivity to template concentration,stability,accuracy,influence of the DNA extraction methods,and the ability to genotype the mixture samples.The performance of the DNATyper^(TM)15 system on casework samples was compared with that of two widely used STR amplification kits,Identifiler^(TM)(Applied Biosystems,Carlsbad,CA,USA)and PowerPlex 16®(Promega,Madison,WI,USA).The conditions for PCR‑based DNATyper^(TM)15 genotyping were optimized.Contamination from forensically relevant nonhuman DNA was not found to impact genotyping results,and full profiles were generated for all the reactions containing≥0.125 ng of DNA template.No significant difference in performance was observed even after the DNATyper^(TM)15 assay components were subjected to 20 freeze‑thaw cycles.The performances of DNATyper^(TM)15,Identifiler^(TM),and PowerPlex 16®were comparable in terms of sensitivity and the ability to genotype the mixed samples and case‑type samples,with the assays giving the same genotyping results for all the shared loci.The DNA extraction methods did not affect the performance of any of the systems.Our results demonstrate that the DNATyper^(TM)15 system is suitable for genotyping in both forensic DNA database work and case‑type samples. | Jian Ye Chengtao Jiang Xingchun Zhao Le Wang Caixia Li Anquan Ji Li Yuan Jing Sun Shuaifeng Chen | 2015 | Journal of Forensic Science and Medicine2015，1，1： | 3 |
| 18 | 先锋氢引燃煤油的超燃燃烧室火焰稳定性研究显示文摘目的:1.在模拟飞行马赫数4.0条件下,通过直连实验研究先锋氢当量比对煤油燃料冲压发动机点火及稳焰的影响;2.通过多种非接触光学测量手段研究超燃冲压发动机内燃烧流场的结构变化和火焰建立等动态过程。创新点:1.通过直连实验发现,适量提高先锋氢当量比有利于发动机点火,且先锋氢的存在是发动机稳燃的前提;2.得到了超燃发动机点火及燃烧不稳定特征的流场。方法:1.通过直连实验,得到监测点压力动态变化数据并对其进行分析(图4和5);2.通过光学测量手段,观测流场及火焰动态变化过程(图6~12)。结论:1.当先锋氢当量比为0.080时,煤油燃料超燃冲压发动机成功点火;当先锋氢当量比为0.040时,点火失败;当先锋氢关闭后,超燃冲压发动机熄火。2.煤油火焰主要集中于凹槽后缘斜坡且结构是非对称的;燃烧是不稳定的且伴随着反复的前后移动。 | Wen SHI Ye TIAN Wan-zhou ZHANG Wei-xin DENG Fu-yu ZHONG Jia-ling LE | 2020 | Journal of Zhejiang University-Science A(Applied Physics & Engineering)2020，21，8： | 3 |
| 19 | Dihydro-stilbene gigantol relieves CCl4-induced hepatic oxidative stress and inflammation in mice via inhibiting C5b-9 formation in the liver显示文摘In general,anti-inflammatory treatment is considered for multiple liver diseases despite the etiology.But current drugs for alleviating liver inflammation have defects,making it necessary to develop more potent and safer drugs for liver injury.in this study,we screened a series of(dihydro-)stillene or(dihydro-)phenanthrene derivatives extracted from Pholidota chinensis for theirpotential biological activities.Among 31 compounds,the dihydro-stlbene gigantol exerted most potent protective effects onhuman hepatocytes against lithocholic acid toxicity,and exhibited solid antioxidative and anti-inflammatory effect in vitro.In micewith CCl-induced acute liver injury,pre-administration of gigantol(10,20,40 mg kg^-1·d^-1,po,for 7 days)dose-dependentydecreased serum transaminase levels and improved pathological changes in liver tissues.The elevated lipid peroxidation andinflammatory responses in the livers were also signifhcantly alleviated by gigantol.The pharmacokinetic studies showed thatgigantol was highly concentrated in the mouse lvers,which consisted with its efficacy in preventing liver injury.Using a label-freequantitative proteomic analysis we revealed that gigantol mainly regulated the immune system process in liver tissues of CCl4-treated mice,and the complement and coagulation cascades was the predominant pathway gigantol markedly inhibited theexpression of complement component C9,which was a key component for the formation of terminal complerment complex(TCC)C5b-9.These results were validated by immunohistochemistry(IHC) or real time-PCR.Confocal microscopy analysis showed thatgigantol significantly inhibited the vascular deposition of TCC in the liver.In conclusion,we demonstrate for the first time that oral administration of gigantol potently relieves liver oxidative stress and inflammation,possibly via a novel mechanism of inhibiting the C5b-9 formation in the liver. | Ya-ru Xue Sheng Yao Qian Liu Zhao-liang Peng Qiang-qiang Deng—Bo Liu Zheng-hua Ma Le Wangf Hu Zhou Yang Ye Guo-yu Pan | 2020 | Acta Pharmacologica Sinica2020，41，11： | 3 |
| 20 | Polydentate cyclotriphosphazene ligands:Design,synthesis and bioactivity显示文摘五多原子的 cyclotriphosphazene ligands 为他们的劈开活动被综合并且测试到原生质标志 DNA。所有被 MS 这些新混合物证实, 1 H NMR, 31 P NMR, 13 C NMR 和红外。pUC19 DNA 的劈开上的初步的研究面对金属建筑群被执行。结果表明这些建筑群能在生理的条件下面充当强大的催化剂。建筑群 3b + Cu 能有效地劈开 DNA 到 nicked 形式,给水解作用在生理的条件下面的 0.08/h 的率常数。acid–基础催化 DNA phosphate-diester 水解作用机制也被建议。 | Le Wang Yong Ye Shang Bin Zhong Yu Fen Zhao | 2009 | Chinese Chemical Letters2009，20，1： | 2 |
您所在的机构暂未开通此部分的功能，请联系管理员