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| 1 | A seven-gene-deleted African swine fever virus is safe and effective as a live attenuated vaccine in pigs显示文摘African swine fever(ASF)is a devastating infectious disease in swine that is severely threatening the global pig industry.An efficacious vaccine is urgently required.Here,we used the Chinese ASFV HLJ/18 as a backbone and generated a series of genedeleted viruses.The virulence,immunogenicity,safety,and protective efficacy evaluation in specific-pathogen-free pigs,commercial pigs,and pregnant sows indicated that one virus,namely HLJ/18-7GD,which has seven genes deleted,is fully attenuated in pigs,cannot convert to the virulent strain,and provides complete protection of pigs against lethal ASFV challenge.Our study shows that HLJ/-18-7GD is a safe and effective vaccine against ASFV,and as such is expected to play an important role in controlling the spread of ASFV. | Weiye Chen Dongming Zhao Xijun He Renqiang Liu Zilong Wang Xianfeng Zhang Fang Li Dan Shan Hefeng Chen Jiwen Zhang Lulu Wang Zhiyuan Wen Xijun Wang Yuntao Guan Jinxiong Liu Zhigao Bu | 2020 | Science China(Life Sciences)2020，63，5： | 72 |
| 2 | Spatiotemporal changes in vegetation coverage and its driving factors in the Three-River Headwaters Region during 2000-2011显示文摘The Three-River Headwaters Region(TRHR), which is the source area of the Yangtze River, Yellow River, and Lancang River, is of key importance to the ecological security of China. Because of climate changes and human activities, ecological degradation occurred in this region. Therefore, 'The nature reserve of Three-River Source Regions' was established, and 'The project of ecological protection and construction for the Three-River Headwaters Nature Reserve' was implemented by the Chinese government. This study, based on MODIS-NDVI and climate data, aims to analyze the spatiotemporal changes in vegetation coverage and its driving factors in the TRHR between 2000 and 2011, from three dimensions. Linear regression, Hurst index analysis, and partial correlation analysis were employed. The results showed the following:(1) In the past 12 years(2000–2011), the NDVI of the study area increased, with a linear tendency being 1.2%/10a, of which the Yangtze and Yellow River source regions presented an increasing trend, while the Lancang River source region showed a decreasing trend.(2) Vegetation coverage presented an obvious spatial difference in the TRHR, and the NDVI frequency was featured by a bimodal structure.(3) The area with improved vegetation coverage was larger than the degraded area, being 64.06% and 35.94%, respectively during the study period, and presented an increasing trend in the north and a decreasing trend in the south.(4) The reverse characteristics of vegetation coverage change are significant. In the future, degradation trends will be mainly found in the Yangtze River Basin and to the north of the Yellow River, while areas with improving trends are mainly distributed in the Lancang River Basin.(5) The response of vegetation coverage to precipitation and potential evapotranspiration has a time lag, while there is no such lag in the case of temperature.(6) The increased vegetation coverage is mainly attributed to the warm-wet climate change and the implementation of the ecological protection project. | LIU Xianfeng ZHANG Jinshui ZHU Xiufang PAN Yaozhong LIU Yanxu ZHANG Donghai LIN Zhihui | 2014 | Journal of Geographical Sciences2014，24，2： | 47 |
| 3 | Emergence and prevalence of naturally occurring lower virulent African swine fever viruses in domestic pigs in China in 2020显示文摘African swine fever virus(ASFV)has been circulating in China for more than two years,and it is not clear whether the biological properties of the virus have changed.Here,we report on our surveillance of ASFVs in seven provinces of China,from June to December,2020.A total of 22 viruses were isolated and characterized as genotype II ASFVs,with mutations,deletions,insertions,or short-fragment replacement occurring in all isolates compared with Pig/HLJ/2018(HLJ/18),the earliest isolate in China.Eleven isolates had four different types of natural mutations or deletion in the EP402R gene and displayed a nonhemadsorbing(non-HAD)phenotype.Four isolates were tested for virulence in pigs;two were found to be as highly lethal as HLJ/18.However,two non-HAD isolates showed lower virulence but were highly transmissible;infection with 106 TCID50 dose was partially lethal and caused acute or sub-acute disease,whereas 103 TCID50 dose caused non-lethal,sub-acute or chronic disease,and persistent infection.The emergence of lower virulent natural mutants brings greater difficulty to the early diagnosis of ASF and creates new challenges for ASFV control. | Encheng Sun Zhenjiang Zhang Zilong Wang Xijun He Xianfeng Zhang Lulu Wang Wenqing Wang Lianyu Huang Fei Xi Haoyue Huangfu Ghebremedhin Tsegay Hong Huo Jianhong Sun Zhijun Tian Wei Xia Xuewu Yu Fang Li Renqiang Liu Yuntao Guan Dongming Zhao Zhigao Bu | 2021 | Science China(Life Sciences)2021，64，5： | 46 |
| 4 | 南京葫芦洞石笋δ^(13)C对冰期气候的复杂响应与诊断显示文摘文中重建了南京葫芦洞内距今75~10ka期间7支石笋δ13C的时间变化序列,δ13C曲线在重叠时段均具有一致性波动形式.在最后一个冰期/间冰期旋回,δ13C值变化幅度达6‰,反映了C3/C4植被类型变化对δ13C的主控作用.然而,在末次冰期格陵兰冰心记录的DO暖事件时(千年尺度),δ13C与指示降水变化的δ18O呈反相关,主要反映了快速渗水条件下洞穴岩溶水对土壤CO2溶解量的降低.据同期生长石笋δ13C和δ18O对比分析,石笋碳同位素动力分馏程度与生长速率有关.进一步研究发现,本区末次冰消期植被类型变化滞后于降水变化约700年. | 孔兴功 汪永进 吴江滢 Cheng Hai L R Edwards Wang Xianfeng | 2005 | 中国科学(D辑)2005，35，11： | 24 |
| 5 | Source Separation of Diesel Engine Vibration Based on the Empirical Mode Decomposition and Independent Component Analysis显示文摘Vibration signals from diesel engine contain many different components mainly caused by combustion and mechanism operations,several blind source separation techniques are available for decomposing the signal into its components in the case of multichannel measurements,such as independent component analysis(ICA).However,the source separation of vibration signal from single-channel is impossible.In order to study the source separation from single-channel signal for the purpose of source extraction,the combination method of empirical mode decomposition(EMD) and ICA is proposed in diesel engine signal processing.The performance of the described methods of EMD-wavelet and EMD-ICA in vibration signal application is compared,and the results show that EMD-ICA method outperforms the other,and overcomes the drawback of ICA in the case of single-channel measurement.The independent source signal components can be separated and identified effectively from one-channel measurement by EMD-ICA.Hence,EMD-ICA improves the extraction and identification abilities of source signals from diesel engine vibration measurements. | DU Xianfeng LI Zhijun BI Fengrong ZHANG Junhong WANG Xia SHAO Kang | 2012 | Chinese Journal of Mechanical Engineering2012，25，3： | 21 |
| 6 | Multi-scale HPC system for multi-scale discrete simulation—Development and application of a supercomputer with 1 Petaflops peak performance in single precision显示文摘A supercomputer with 1.0 Petaflops peak performance in single precision,designed and established by Institute of Process Engineering,Chinese Academy of Sciences,is introduced in this brief communication.A designing philosophy utilizing the similarity between hardware,software and the problems to be solved is embodied,based on the multi-scale method and discrete simulation approaches developed at Institute of Process Engineering(IPE)and implemented in a graphic processing unit(GPU)-based hybrid computing mode.The preliminary applications of this machine in areas of multi-phase flow,molecular dynamics and so on are reported,demonstrating the supercomputer as a paradigm of green computation in new architecture. | Feiguo Chen Wei Ge Li Guo Xianfeng He Bo Li Jinghai Li Xipeng Li Xiaowei Wang Xiaolong Yuan | 2009 | Particuology2009，7，4： | 19 |
| 7 | Quasi-real-time simulation of rotating drum using discrete element method with parallel GPU computing显示文摘Real-time simulation of industrial equipment is a huge challenge nowadays. The high performance and fine-grained parallel computing provided by graphics processing units (GPUs) bring us closer to our goals. In this article, an industrial-scale rotating drum is simulated using simplified discrete element method (DEM) without consideration of the tangential components of contact force and particle rotation. A single GPU is used first to simulate a small model system with about 8000 particles in realtime, and the simulation is then scaled up to industrial scale using more than 200 GPUs in a 1D domain-decomposition parallelization mode. The overall speed is about 1/11 of the real-time. Optimization of the communication part of the parallel GPU codes can speed up the simulation further, indicating that such real-time simulations have not only methodological but also industrial implications in the near future. | Ji Xu Huabiao Oi Xiaojian Fang Liqiang Lu Wei Ge Xiaowei Wang Ming xu Feiguo Chen xianfeng He Jinghai Li | 2011 | Particuology2011，9，4： | 16 |
| 8 | An experimental study on stress-strain behavior and constitutive model of hardfill material显示文摘Hardfill is a new type of artificially cemented material for dam construction works,with a wide application prospect.Its mechanical behavior lies between concrete and rockfill materials.A series of large-scale triaxial tests are performed on hardfill specimens at different ages,and the stress-strain behavior of hardfill is further discussed.The strength and stress-strain relationship of hardfill materials show both frictional mechanism and cohesive mechanism.An age-related constitutive model of hardfill is developed,which is a parallel model consisting of two components,rockfill component and cementation component.Moreover,a comparison is made between the simulated and the experimental results,which shows that the parallel model can reflect the mechanical characteristics of both rockfill-like nonlinearity and concrete-like age relativity.In addition,a simplified method for the determination of parameters is proposed. | WU MengXi DU Bin YAO YuanCheng HE XianFeng | 2011 | Science China(Physics,Mechanics & Astronomy)2011，54，11： | 15 |
| 9 | Detecting soil salinity with arid fraction integrated index and salinity index in feature space using Landsat TM imagery显示文摘Modeling soil salinity in an arid salt-affected ecosystem is a difficult task when using remote sensing data because of the complicated soil context(vegetation cover,moisture,surface roughness,and organic matter)and the weak spectral features of salinized soil.Therefore,an index such as the salinity index(SI)that only uses soil spectra may not detect soil salinity effectively and quantitatively.The use of vegetation reflectance as an indirect indicator can avoid limitations associated with the direct use of soil reflectance.The normalized difference vegetation index(NDVI),as the most common vegetation index,was found to be responsive to salinity but may not be available for retrieving sparse vegetation due to its sensitivity to background soil in arid areas.Therefore,the arid fraction integrated index(AFII)was created as supported by the spectral mixture analysis(SMA),which is more appropriate for analyzing variations in vegetation cover(particularly halophytes)than NDVI in the study area.Using soil and vegetation separately for detecting salinity perhaps is not feasible.Then,we developed a new and operational model,the soil salinity detecting model(SDM)that combines AFII and SI to quantitatively estimate the salt content in the surface soil.SDMs,including SDM1 and SDM2,were constructed through analyzing the spatial characteristics of soils with different salinization degree by integrating AFII and SI using a scatterplot.The SDMs were then compared to the combined spectral response index(COSRI)from field measurements with respect to the soil salt content.The results indicate that the SDM values are highly correlated with soil salinity,in contrast to the performance of COSRI.Strong exponential relationships were observed between soil salinity and SDMs(R2>0.86,RMSE<6.86)compared to COSRI(R2=0.71,RMSE=16.21).These results suggest that the feature space related to biophysical properties combined with AFII and SI can effectively provide information on soil salinity. | Fei WANG Xi CHEN GePing LUO JianLi DING XianFeng CHEN | 2013 | Journal of Arid Land2013，5，3： | 14 |
| 10 | Bioptical microcycles of laminated speleothems from China and their chronological significance显示文摘Interfaces of microlayers, in most stalagmites from China, consist of opaque and organic materials. Observed with microscope, the interfaces seem dark under transmitted light and luminiferous under reflected fluorescent light. Stalagmites in which the two optical characters are unified spatially would be most suitable for cross-dating use, and can play an important role in the study on speleochronology and speleoclimatology. | Ming Tan Xiaoguang Qin Linmei Shen Xianfeng Wang Yongjin Wang Tieying Li Linhua Song Laihong Wang Dongsheng Liu | 1999 | Chinese Science Bulletin1999，44，17： | 14 |
| 11 | The Interactions among DWARF10,Auxin and Cytokinin Underlie Lateral Bud Outgrowth in Rice显示文摘Previous studies have shown that DWARF10(D10) is a rice ortholog of MAX4/RMS1/DAD1,encoding a carotenoid cleavage dioxygenase and functioning in strigolactones/strigolactone-derivatives(SL) biosynthesis.Here we use D10-RNA interference(RNAi) transgenic plants similar to d10 mutant in phenotypes to investigate the interactions among D10,auxin and cytokinin in regulating rice shoot branching.Auxin levels in node 1 of both decapitated D10-RNAi and wild type plants decreased significantly,showing that decapitation does reduce endogenous auxin concentration,but decapitation has no clear effects on auxin levels in node 2 of the same plants.This implies that node 1 may be the location where a possible interaction between auxin and D10 gene would be detected.D10 expression in node 1 is inhibited by decapitation,and this inhibition can be restored by exogenous auxin application,indicating that D10 may play an important role in auxin regulation of SL.The decreased expression of most OsPINs in shoot nodes of D10-RNAi plants may cause a reduced auxin transport capacity.Furthermore,effects of auxin treatment of decapitated plants on the expression of cytokinin biosynthetic genes suggest that D10 promotes cytokinin biosynthesis by reducing auxin levels.Besides,in D10-RNAi plants,decreased storage cytokinin levels in the shoot node may partly account for the increased active cytokinin contents,resulting in more tillering phenotypes. | Shuying Zhang Gang Li Jun Fang Weiqi Chen Haipai Jiang Junhuang Zou Xue Liu Xianfeng Zhao Xiaobing Li Chengcai Chu Qi Xie Xiangning Jiang Lihuang Zhu | 2010 | Journal of Integrative Plant Biology2010，52，7： | 14 |
| 12 | Characterization and fine mapping of the rice blast resistance gene Pia显示文摘Blast,caused by Magnaporthe oryzae,is one of the most widespread and destructive diseases of rice.Breeding durable resistant cultivars(cvs) can be achieved by pyramiding of various resistance(R) genes.Pia,carried by cv.Aichi Asahi,was evaluated against 612 isolates of M.oryzae collected from 10 Chinese provinces.The Pia gene expresses weak resistance in all the provinces except for Jiangsu.Genomic position-ready marker-based linkage analysis was carried out in a mapping population consisting of 800 F2 plants derived from a cross of Aichi Asahi×Kasalath.The locus was defined in an interval of approximately 90 kb,flanked by markers A16 and A21.Four candidate genes(Pia-1,Pia-2,Pia-3,and Pia-4) ,all having the R gene conserved structure,were predicted in the interval using the cv.Nipponbare genomic sequence.Four candidate resistance gene(CRG) markers(A17,A25,A26,and A27) ,derived from the four candidates,were subjected to genotyping with the recombinants detected at the flanking markers.The first three markers completely co-segregated with the Pia locus,and the fourth was absent in the Aichi Asahi genome and disordered with the Pia locus and its flanking markers,indicating that the fourth candidate gene,Pia-4,could be excluded.Co-segregation marker-based genotyping of the three sets of differentials with known R gene genotypes revealed that the genotype of A26(Pia-3) perfectly matched the R gene genotype of Pia,indicating that Pia-3 is the strongest candidate gene for Pia. | ZENG XiaoShan YANG XianFeng ZHAO ZhengHong LIN Fei WANG Ling PAN QingHua | 2011 | Science China(Life Sciences)2011，54，4： | 13 |
| 13 | Review of Hydrogen Embrittlement in Metals: Hydrogen Diffusion, Hydrogen Characterization, Hydrogen Embrittlement Mechanism and Prevention显示文摘Hydrogen dissolved in metals as a result of internal and external hydrogen can affect the mechanical properties of the metals, principally through the interactions between hydrogen and material defects. Multiple phenomena such as hydrogen dissolution, hydrogen diffusion, hydrogen redistribution and hydrogen interactions with vacancies, dislocations, grain boundaries and other phase interfaces are involved in this process. Consequently, several hydrogen embrittlement(HE) mechanisms have been successively proposed to explain the HE phenomena, with the hydrogen-enhanced decohesion mechanism, hydrogenenhanced localized plasticity mechanism and hydrogen-enhanced strain-induced vacancies being some of the most important. Additionally, to reduce the risk of HE for engineering structural materials in service, surface treatments and microstructural optimization of the alloys have been suggested. In this review, we report on the progress of the studies on HE in metals, with a particular focus on steels. It focuses on four aspects:(1) hydrogen diffusion behavior;(2) hydrogen characterization methods;(3) HE mechanisms;and(4) the prevention of HE. The strengths and weaknesses of the current HE mechanisms and HE prevention methods are discussed, and specific research directions for further investigation of fundamental HE mechanisms and methods for preventing HE failure are identified. | Xinfeng Li Xianfeng Ma Jin Zhang Eiji Akiyama Yanfei Wang Xiaolong Song | 2020 | Acta Metallurgica Sinica(English Letters)2020，33，6： | 13 |
| 14 | Stability analysis of liver cancer-related microRNAs显示文摘MicroRNAs (miRNAs ) 是非编码的, 22 nt 的搁浅单人赛的 RNA 并且组成在植物和动物被发现的基因管理者的一个新奇的班。几研究证明了浆液 miRNAs 能为各种各样的癌症和另外的疾病的察觉用作潜在的 biomarkers。关于肝的稳定性的一些文件在浆液的癌症相关的 miRNAs 是可得到的。在浆液的 miRNA 的稳定性的全身的分析是相当必要的。这研究的目的是在不同试验性的条件下面从三不同来源,有教养的肝癌症 Huh-7 房间行,临床的肝癌症,和浆液评估 miRNAs 的稳定性,包括不同温度,时间持续时间, pH 价值, RNase A 消化, DNase 我消化,并且各种各样的 freezethaw 骑车。qRT-PCR 分析表明了癌症相关的 miRNAs 是的那个肝在每测试条件下面可检测,显示 miRNAs 在严厉环境条件下面对破坏和降级极其稳定、抵抗。然而, ribosomal RNA 由在非生理的测试条件下面表明相对表示的锋利的减少易碎、容易降级。我们也极大地为浆液 RNA 抽取建立了一个柔韧的过程,它是不仅为介绍研究的 miRNA 而且为疾病预后重要基于反常 miRNA 表示。 | Yan Li Zhenggang Jiang Lijian Xu Hu Yao Jiangfeng Guo Xianfeng Ding | 2011 | Acta Biochimica et Biophysica Sinica2011，43，1： | 12 |
| 15 | Enhanced debromination of 4-bromophenol by the UV/sulfite process:Efficiency and mechanism显示文摘Halogenated aromatic compounds have attracted increasing concerns due to their toxicity and persistency in the environment, and dehalogenation is one of the promising treatment and detoxification methods. Herein, we systematically studied the debromination efficiency and mechanism of para-bromophenol(4-BP) by a recently developed UV/sulfite process. 4-BP underwent rapid degradation with the kinetics accelerated with the increasing sulfite concentration, pH(6.1–10) and temperature, whereas inhibited by dissolved oxygen and organic solvents. The apparent activation energy was estimated to be 27.8 kJ/mol. The degradation mechanism and pathways of 4-BP were explored by employing N_2O and nitrate as the electron scavengers and liquid chromatography/mass spectrometry to identify the intermediates. 4-BP degradation proceeded via at least two pathways including direct photolysis and hydrated electron-induced debromination. The contributions of both pathways were distinguished by quantifying the quantum yields of 4-BP via direct photolysis and hydrated electron production in the system. 4-BP could be readily completely debrominated with all the substituted Br released as Br-, and the degradation pathways were also proposed. This study would shed new light on the efficient dehalogenation of brominated aromatics by using the UV/sulfite process. | Bihuang Xie Xuchun Li Xianfeng Huang Zhe Xu Weiming Zhang Bingcai Pan | 2017 | Journal of Environmental Sciences2017，29，4： | 12 |
| 16 | Synthesis of a novel triple-site diphosphinoamine (PNP) ligand and its applications in ethylene tetramerization显示文摘A novel triple-site diphosphinoamine (PNP) ligand was synthesized and characterized. In combination with Cr(III) and methylaluminoxane (MAO), it generated active catalytic systems for ethylene tetramerization toward 1-octene with high catalytic activity and long lifetime. The effects of reaction temperature, molar ratio of Al/Cr and molar ratio of ligand/Cr on catalytic activity and selectivity to 1-octene were studied with reaction kinetics of the catalytic system for ethylene tetramerization described. At the Al/Cr molar ratio of 100, the catalytic activity is up to 2.29×106 g·mol-1 (Cr)·h-1 and the selectivity to 1-octene is 49.65 wt%. | MAO GuoLiang NING YingNan HU WenBo LI ShanMing SONG XianFeng NIU Bo JIANG Tao | 2008 | Chinese Science Bulletin2008，53，22： | 11 |
| 17 | Spatiotemporal changes of cold surges in Inner Mongolia between 1960 and 2012显示文摘在这研究,我们在内部蒙古和包围区域从 121 个气象学的车站用每日的最小的温度数据在在 1960 和 2012 之间的内部蒙古和他们的可能的开车因素分析了冷巨浪的空间与时间的变化。这些数据被分析利用一个 piecewise 回归模型,一个 Sen+Mann-Kendall 模型,和关联分析。结果证明(1 ) 单个车站的冷巨浪的频率在学习时期期间在内部蒙古减少了,与 0.5 times/10a (2.4 ~ 1.2 times/10a ) 的一个线性趋势。在 1991 以前, 1.1 times/10a (3.3 ~ 2.5 times/10a ) 的一个重要减少趋势被检测,当 0.45 times/10a (4.4 ~ 4.2 times/10a ) 的一个增加的趋势在 1991 以后被发现时。在季节的规模上,在春天寒冷巨浪的趋势与年度价值一致,并且在冷巨浪的最明显的变化发生在春天期间。每月冷的巨浪频率显示了 bimodal 结构,并且 11 月见证冷巨浪的最高的发生。(2 ) 空间地,冷巨浪的高发生主要在内部蒙古的北、中央的部分被观察,与观察在的更高的出现比在中央部分北。内部十的特征也揭示了那高频率,分别地,低频率区域在 1960 和 1990 之间介绍了减少并且增加的趋势。在 1990 年代以后扩展的高频率区域,和展出高冷的巨浪频率的区域主要在 Tulihe, Xiaoergou,和 Xi Ujimqin 旗帜被散布。(3 ) 在年度规模上,冷巨浪被 AO, NAO, CA, APVII,和 CQ 统治。然而,在冷巨浪的驱动力的季节的差别被检测。冬季冷巨浪显著地与 AO, NAO,史, CA, TPI, APVII, CW,和 IZ 被相关,显示他们被多重因素引起。秋天冷巨浪被 CA 和 IM 主要影响,当春天寒冷巨浪显著地与 CA 和 APVII 被相关时。 | LIU Xianfeng | 2015 | Journal of Geographical Sciences2015，25，3： | 11 |
| 18 | Influence of moxibustion temperatures on blood lipids, endothelin-1, and nitric oxide in hyperlipidemia patients显示文摘Abstract OBJECTIVE:To observe the influence of moxibustion temperature on blood lipids,endothelin-1(ET-1),nitric oxide(NO),and ET-1/NO in hyperlipidemia patients.METHODS:Forty-two primary hyperlipidemia patients were randomly divided into two groups of 21and treated with moxibustion at different temperatures.Moxibustion was performed with the moxa roll 2.5-3.0 cm from the skin in the treatment group and 4 cm in the control group,10 min per point,once every other day.Skin temperature was precisely measured with a thermometer during moxibustion.After a 12-week treatment,seven measurements of blood lipids,ET-1,and NO were recorded.RESULTS:Total cholesterol and triglyceride,were lower in the treatment group than in the control group(P<0.05).Serum ET-1 and ET-1/NO was obvi-ously lowered in the treatment group(P<0.001).Moxibustion regulated NO and ET-1/NO in the treatment group much better than in the control group.CONCLUSION:Moxibustion can regulate blood lipids and clear blood vessels.Moxibustion at45℃has a better effect than moxibustion at 38℃on regulating blood lipids and protecting vascular endothelial function,indicating that suitable temperature influences the curative effect of moxibustion. | Xianfeng Ye Huifang Zhang | 2013 | Journal of Traditional Chinese Medicine2013，33，5： | 11 |
| 19 | A Geometric Understanding of Deep Learning显示文摘This work introduces an optimal transportation(OT)view of generative adversarial networks(GANs).Natural datasets have intrinsic patterns,which can be summarized as the manifold distribution principle:the distribution of a class of data is close to a low-dimensional manifold.GANs mainly accomplish two tasks:manifold learning and probability distribution transformation.The latter can be carried out using the classical OT method.From the OT perspective,the generator computes the OT map,while the discriminator computes the Wasserstein distance between the generated data distribution and the real data distribution;both can be reduced to a convex geometric optimization process.Furthermore,OT theory discovers the intrinsic collaborative-instead of competitive-relation between the generator and the discriminator,and the fundamental reason for mode collapse.We also propose a novel generative model,which uses an autoencoder(AE)for manifold learning and OT map for probability distribution transformation.This AE–OT model improves the theoretical rigor and transparency,as well as the computational stability and efficiency;in particular,it eliminates the mode collapse.The experimental results validate our hypothesis,and demonstrate the advantages of our proposed model. | Na Lei Dongsheng An Yang Guo Kehua Su Shixia Liu Zhongxuan Luo Shing-Tung Yau Xianfeng Gu | 2020 | Engineering2020，6，3： | 10 |
| 20 | The WRKY transcription factor PlWRKY65 enhances the resistance of Paeonia lactiflora(herbaceous peony)to Alternaria tenuissima显示文摘In this study,the disease resistance gene PlWRKY65 was isolated from the leaves of Paeonia lactiflora and analyzed by bioinformatics methods,and the localization of the encoded protein was explored.Quantitative real-time PCR(qRTPCR)was also used to explore the response of this gene to Alternaria tenuissima.The results showed that the gene sequence contained multiple cis-acting elements involved in the response to hormone signaling molecules belonging to the IIe subgroup of the WRKY family,and the encoded proteins were located in the nucleus.The PlWRKY65 gene has a positive regulatory effect on A.tenuissima infection.After silencing the PlWRKY65 gene via virus-induced gene silencing(VIGS),it was found that the gene-silenced plants were more sensitive to A.tenuissima infection than the wild plants,exhibiting more severe infection symptoms and different degrees of changes in the expression of the pathogenesis-related(PR)genes.In addition,we showed that the endogenous jasmonic acid(JA)content of P.lactiflora was increased in response to A.tenuissima infection,whereas the salicylic acid(SA)content decreased.After PlWRKY65 gene silencing,the levels of the two hormones changed accordingly,indicating that PlWRKY65,acting as a disease resistance-related transcriptional activator,exerts a regulatory effect on JA and SA signals.This study lays the foundation for functional research on WRKY genes in P.lactiflora and for the discovery of candidate disease resistance genes. | Xue Wang Junjie Li Jing Guo Qian Qiao Xianfeng Guo Yan Ma | 2020 | Horticulture Research2020，7，1： | 10 |
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