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| 1 | Template‐Assisted Synthesis of Mesoporous Magnetic Nanocomposite Particles显示文摘 | Wu Pinggui Zhu Jianhua Xu Zhenghe | 2004 | Adv Funct Mater2004，14，4： | 1 |
| 2 | Visible-light-induced photocatalytic inactivation of bacteria by composite photocatalysts of palladium oxide and nitrogen-doped titanium oxide显示文摘 | Pinggui Wu Rongcai Xie James A. Imlay Jian Ku Shang | 2008 | Applied Catalysis B, Environmental2008，，3： | 1 |
| 3 | 显示文摘 | Zhang Hongguo Li Longtu Wu Pinggui | 2000 | Materials Research Bulletin2000，35，： | 1 |
| 4 | Investigation on structure and properties of low-temperature sintered composite ferrites显示文摘 | Zhang Hongguo Li Longtu Wu Pinggui | 2000 | Mater Res Bull2000，35，： | 1 |
| 5 | Physiological and Biochemical Indexes of Tibetan Sheep at Different Months of Age显示文摘To study the changes in physiological and biochemical indexes of Tibetan sheep from the plateau area at different months of age,we measured the physiological and biochemical indexes of Tibetan sheep reared in Hongyuan County of Sichuan Province according to their growth stages. The results showed that the respiration,body temperature and heart rate of Tibetan sheep at 3- 24 months of age were( 24. 60 ± 6. 33)-( 50. 30 ± 21. 18) times / min,( 39. 14 ± 0. 28) ℃-( 39. 64 ± 0. 17) ℃ and( 91. 00 ± 13. 27)-( 112. 00 ± 8. 69) times/min,respectively. The blood physiological indexes of Tibetan sheep including white blood cell count( WBC),hematocrit( HCT) and red blood cell distribution width coefficient of variation( RDW- CV) at 3,6,15 and 18 months of age were relatively lower than those at 9,12,21 and 24 months of age. The blood physiological indexes including red blood cell count( RBC),mean corpuscular capacity( MCV),mean corpuscular hemoglobin concentration( MCHC) and mean corpuscular hemoglobin( MCH) at 3,6,15 and 18 months of age were relatively higher than those at 9,12,21 and 24 months of age,while hemoglobin( HGB) and platelet count( PLT) did not vary with months of age. For blood biochemical indexes,alanine aminotransferase( ALT),albumin( ALB),globulin( GLO) and total cholesterol( CHOL) reached the maximum values at 21 months of age; alkaline phosphatase( ALKP),lactate dehydrogenase( LDH),blood glucose( GLU) and pseudocholinesterase( PCHE) reached the maximum values at 3 months of age;and total calcium( CA),total protein( TP),aspartate aminotransferase( AST) reached the maximum values at 9,18 and 24 months of age,respectively. | Zhou Mingliang Jiang Shihai Chen Minghua Yang Pinggui Wu Weisheng Xie Rongqing | 2015 | Animal Husbandry and Feed Science2015，7，6： | 0 |
| 6 | Seasonal Changes of Physiological and Biochemical Indexes of Tibetan Sheep显示文摘To investigate the seasonal changes in physiological and biochemical indexes of Tibetan sheep in Hongyuan area,we measured the physiological and biochemical indexes of Tibetan sheep at four different seasons. The results showed that the body temperature,respiration and heart rate of Tibetan sheep at four seasons were 39. 18- 39. 60 ℃,27. 20- 49. 04 times / min,91. 87- 106. 25 times / min,respectively. The indexes of WBC,MCV,MCH and MCHC in autumn and winter were significantly higher than those in spring and summer( P < 0. 01),while the indexes of RBC,HCT and RDW-CV in spring and summer were significantly higher than those in autumn and winter( P < 0. 01). The PLT did not vary greatly in spring,summer or autumn,but were all extremely higher than that in winter( P< 0. 01). The HGB maintained stable in the four seasons. The indexes of TP,ALB and GLO in summer and autumn were extremely higher than those in winter and spring( P < 0. 01),while the indexes of ALP and PCHE in summer and autumn were extremely lower than those in winter and spring( P < 0. 01). The indexes of AST,ALT,LDH,GLU,CHOL and CA maintained stable in all seasons. | Zhou Mingliang Chen Minghua Wu Weisheng Xie Rongqing Jiang Shihai Yang Pinggui | 2016 | Animal Husbandry and Feed Science2016，8，4： | 0 |
| 7 | Analysis of Physiological and Biochemical Indexes of White-tibetan Sheep at Different Months of Age显示文摘To study the physiological and biochemical indexes of White-tibetan sheep in plateau environment,we measured the physiological and biochemical indexes of White-tibetan sheep at 3,6,9,12,15,18,21 and 24 months of age. The results showed that the body temperature,respiration and heart rate of White-tibetan sheep at 3- 24 months of age were( 38. 89 ± 0. 56)-( 39. 83 ± 0. 32) ℃,( 26. 00 ± 6. 67)-( 58. 64 ± 12. 67) times / min and( 83. 90 ± 7. 64)-( 111. 36 ±7. 46) times/min,respectively. The blood physiological indexes of WBC,HCT and RDW-CV at 3,6,15 and 18 months of age were lower than those at 9,12,21 and 24 months of age. On the contrary,the indexes of RBC,MCV,MCH and MCHC at 3,6,15 and 18 months of age were higher than those at 9,12,21 and 24 months of age,while HGB and PLT did not vary with months of age. For blood biochemical indexes,ALT,AST,TP,ALB,LDH and CHOL reached the maximums at 21 months of age,while ALP,PCHE and GLU reached the maximums at 3 months of age. Besides,the GLO and GA reached the maximums at 18 and 24 months of age,respectively. The physiological and biochemical indexes of White-tibetan sheep followed certain rules in growth and development process,and might be affected by plateau climate and season. | Zhou Mingliang Chen Minghua Wu Weisheng Xie Rongqing Jiang Shihai Yang Pinggui | 2016 | Animal Husbandry and Feed Science2016，8，2： | 0 |
| 8 | Analysis of Physiological and Biochemical Indexes of White Suffolk Sheep,White-tibetan Sheep and Tibetan Sheep显示文摘In order to compare the physiological and biochemical indexes of different sheep populations in plateau area,we measured the physiological and biochemical indexes of White Suffolk sheep,White-tibetan sheep and Tibetan sheep in four seasons.The body temperature,respiration and heart rate of White Suffolk sheep,White-tibetan sheep and Tibetan sheep throughout the year were(38.96±0.23)-(40.80±0.74)℃,(27.20±7.73)-(74.56±10.91)times/min,and(83.10±2.42)-(106.25±7.07)times/min,respectively.The main blood physiological indexes of White-tibetan sheep were between those of Suffolk sheep and Tibetan sheep or close to that of Tibetan sheep,the indexes of WBC in summer,HGB and RDW-CV in autumn,RDW-CV in winter were close to that of White Suffolk sheep.The main blood biochemical indexes of White-tibetan sheep were between that of White Suffolk sheep and Tibetan sheep or close to that of Tibetan sheep,but the indexes of ALB in spring,AST in summer,ALP and CHOL in autumn,and ALP in winter were close to that of White Suffolk sheep.It revealed that the adaptability of White-tibetan sheep was prior to White Suffolk sheep,and tended to Tibetan sheep in the plateau environment. | Zhou Mingliang Chen Minghua Wu Weisheng Xie Rongqing Jiang Shihai Yang Pinggui | 2016 | Animal Husbandry and Feed Science2016，8，3： | 0 |
| 9 | Cloning and Sequence Analysis of IGFBP-1 Gene in Sheep显示文摘In this study,using Liangshan semi-fine wool sheep as an experimental material,full-length CDS sequence of IGFBP-1 gene was cloned with RT-PCR method and analyzed with bioinformatics methods. The results showed that the full-length CDS sequence of IGFBP-1 gene in Liangshan semi-fine wool sheep was792 bp in length,encoding 263 amino acids. The CDS sequence shared 97%,76% and 74% homology with bovine,human and rat,respectively; the amino acid sequence shared 97%,69% and 71%,respectively. The Gen Bank accession number was FJ589639. 1. The amino acid molecular weight of IGFBP-1 was 27. 8k D,and the theoretical isoelectric point( p I) was 5. 99. The result of phylogenetic analysis showed that IGFBP-1 gene in Liangshan semi-fine wool sheep exhibited close phylogenetic relationships with bovine,goat and other mammals,and distant phylogenetic relationships with chicken and fish. IGFBP-1 gene had obvious hydrophobic and hydrophilic regions,harboring one signal peptide,one transmembrane region,16 phosphorylation sites,six N-glycosylation sites and eight O-glycosylation sites. The result of secondary structure analysis showed that the random coil,α-helix and β-sheet regions accounted for 61. 98%,24. 33% and 13. 69%,respectively. The result of tertiary structure analysis showed that IGFBP-1 harbors an IGFBP_N domain and a thyroglobulin type-I domain. This study laid a solid foundation for further investigating the function of IGFBP-1 gene in sheep. | Mingliang ZHOU Pinggui YANG Dengjun WU Xiangyu ZHANG | 2015 | Agricultural Biotechnology2015，4，5： | 0 |
| 10 | Cloning and Sequence Analysis of IGFBP-7 Gene in Sheep显示文摘In this study,full-length CDS sequence of IGFBP-7 gene was cloned from Liangshan semi-fine wool sheep with RT-PCR method and analyzed with bioinformatics methods. The results showed that the full-length CDS sequence of IGFBP-7 gene in Liangshan semi-fine wool sheep was 846 bp in length,encoding282 amino acids. The CDS sequence shared 99%,95% and 90% homology with bovine,human and rat,respectively; the amino acid sequence shared 98%,93%and 89%,respectively. The GenB ank accession number was FJ589640. 1. The amino acid molecular weight of IGFBP-7 was 29. 0 kD,and the theoretical isoelectric point( pI) was 8. 25. The result of phylogenetic analysis showed that IGFBP-7 gene in Liangshan semi-fine wool sheep exhibited close phylogenetic relationships with bovine,goat and other mammals,and distant phylogenetic relationships with Danio rerio and Haliotis diversicolor. IGFBP-7 gene had uniformly distributed hydrophobic and hydrophilic regions,harboring one signal peptide,two transmembrane regions,16 phosphorylation sites,four N-glycosylation sites and one O-glycosylation site. The result of secondary structure analysis showed that the random coil,α-helix and β-sheet regions accounted for 64. 89%,19. 86% and 15. 25%,respectively. The result of tertiary structure analysis showed that IGFBP-7 harbors an IGFBP_N domain and an Ig-like domain. This study provided scientific basis for further investigating the function of IGFBP-7 gene in sheep. | Mingliang ZHOU Pinggui YANG Dengjun WU Xiangyu ZHANG | 2015 | Agricultural Biotechnology2015，4，6： | 0 |
| 11 | Expression Pattern of IGF-I Gene and Correlation between Its SNPs and Growth Traits in Sheep显示文摘This study was to investigate the expression pattern of IGF-I gene and correlation between its SNPs and growth traits in sheep.The expression patterns of IGF-I gene in brain,muscle,skin,liver and heart of Liangshan semi-wool sheep were detected at six growth stages(15,60,105,195,240 days) by employing qRT-PCR approach,and the correlation between SNPs of IGF-I gene and growth traits via SSCP method.The first inflection point of IGF-I gene expression was observed at day 105 in all five tissues.From 15-105 days IGF-I gene gave a decline-ascending trend in muscle,brain and liver,while it did not vary significantly during day 15-day 60 and was up-regulated in heart and liver at day 105.Whenafter,the expression level was down-regulated in liver,while that in muscle,heart,brain and skin assumed the second inflection point.The expression levels of IGF-I gene in brain and skin at day 195 were significantly higher than that at day 150 and day 240.These suggest the synchronous expression of IGF-I gene in all five tissues.Two SNPs located at the leader region and exon 3 of IGF-I gene were middle or low polymorphic.The SNP locus detected with primer P-1 was significantly correlated with birth weight of Liangshan semi-wool sheep(P <0.05),while that detected with primer P-2 was significantly correlated with weaning weight and the weaning daily gain(P < 0.05).The expression of IGF-I in all tissues had the similar developmental patterns,and strong correlation of the SNPs of IGF-I gene was confirmed with the eary growth traits to provide theoretical basis for the growth regulation and applying to assisted selective breeding with the SNPs in liangshan semi-wool sheep. | Zhou Mingliang Zhang Xiangyu Qi Songzhi Wang Qi Wu Dengjun Yang Pinggui | 2015 | Animal Husbandry and Feed Science2015，7，1： | 0 |
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