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| 1 | Lx2-32c,a novel semi-synthetic taxane,exerts antitumor activity against prostate cancer cells in vitro and in vivo显示文摘Tubulin has been shown to be an effective target for the development of cytotoxic agents against prostate cancer. Previously, we reported that Lx2-32 c is an anti-tubulin agent with high binding affinity to tubulin. In this study, we investigated the potential of Lx2-32 c to act as an effective cytotoxic agent in the treatment of prostate cancer. MTT assays showed that Lx2-32 c was cytotoxic to all tested prostate cancer cell lines. The Lx2-32c-treated cells typically exhibited a rounded morphology associated with the onset of apoptosis, as evidenced by immunocytochemical staining. Human prostate cancer cell lines treated with Lx2-32 c arrest in the G2/M phase of the cell cycle and the treatment is associated with an increased ratio of cells in the sub-G0/G1 phase as determined by flow cytometry. Furthermore, expression of the cleaved form of poly(ADP-ribose) polymerase in prostate cancer cell lines treated with Lx2-32 c was shown by Western blotting assay. Xenograft implants of LNCa P and PC3-derived tumors in nude mice showed that Lx2-32 c treatment significant inhibited tumor growth with effects equivalent to those of docetaxel. These findings demonstrate the potential of Lx2-32 c as a candidate antitumor agent for the treatment of prostate cancer. | Guangyao Lv Dengjun Sun Jingwen Zhang Xiaoxia Xie Xiaoqiong Wu Weishuo Fang Jingwei Tian Chunhong Yan Hongbo Wang Fenghua Fu | 2017 | Acta Pharmaceutica Sinica B2017，7，1： | 6 |
| 2 | Strategies of Gene Mutation Detection 显示文摘 | Zhang Ming Wu Dengjun Zheng Hongpei | 2003 | Tren Life Sci2003，12，： | 1 |
| 3 | Review of ultraviolet ageing mechanisms and anti-ageing methods for asphalt binders显示文摘Asphalt binder is inevitably aged by ultraviolet(UV)light during its service period.UV ageing can significantly decrease the technical properties of asphalt binder.The sensitivity of asphalt to UV ageing and thermal-oxidative ageing differs,such that the UV ageing performance cannot be determined based on the thermal-oxidative ageing performance.Previous researches mainly focused on the chemical composite and technical performance changes of asphalt binder during UV ageing,and the UV light parameters effect on the ageing rate of asphalt binder.However,the theory for characterizing and explaining the development of UV ageing depth does not get too much attentions,and the UV ageing mechanism of asphalt binder is not very clear.Therefore,it cannot guide to develop or select the good methods or anti-UV ageing additives for asphalt binders.This paper focuses on the latest researches of the mechanisms and anti-ageing methods of asphalt binders.With the increase of UV ageing time,the UV ageing of asphalt binder develops gradually from the surface to inner part.There are various methods,such as low-penetration grade asphalt,less air void ratio,UV stabilizers and UV light absorbers,that can improve the UV ageing resistance of asphalt binders.A new theory of sensitive wavelengths of asphalt UV ageing is proposed,which can enrich the basic theory of asphalt UV ageing.Depending on this theory,different wavelengths of UV light have different ageing effects on asphalt binder.The composite anti-UV ageing additives with barrier and specific absorption effects on UV light is proposed,and may have better improvement effect on the anti-UV ageing performance of asphalt binder. | Yuanyuan Li Jianlin Feng Shaopeng Wu Anqi Chen Dongliang Kuang Tao Bai Yangming Gao Jizhe Zhang Linglin Li Liyao Wan Quantao Liu Zongwu Chen Dengjun Gu | 2022 | Journal of Road Engineering2022，2，2： | 1 |
| 4 | Quantification of the redox properties of microplastics and their effect on arsenite oxidation显示文摘Microplastics have attracted global concern.The environmental-weathering processes control their fate,transport,transformation,and toxicity to wildlife and human health,but their impacts on biogeochemical redox processes remain largely unknown.Herein,multiple spectroscopic and electrochemical approaches in concert with wet-chemistry analyses were employed to characterize the redox properties of weathered microplastics.The spectroscopic results indicated that weathering of phenol-formaldehyde resins(PFs)by hydrogen peroxide(H2O2)led to a slight decrease in the content of phenol functional groups,accompanied by an increase in semiquinone radicals,quinone,and carboxylic groups.Electrochemical and wet-chemistry quantifications,coupled with microbial-chemical characterizations,demonstrated that the PFs exhibited appreciable electron-donating capacity(0.264-1.15 mmol e-g^(-1))and electron-accepting capacity(0.120-0.300 mmol e-g^(-1)).Specifically,the phenol groups and semiquinone radicals were responsible for the electron-donating capacity,whereas the quinone groups dominated the electron-accepting capacity.The reversible redox peaks in the cyclic voltammograms and the enhanced electron-donating capacity after accepting electrons from microbial reduction demonstrated the reversibility of the electron-donating and-accepting reactions.More importantly,the electron-donating phenol groups and weathering-induced semiquinone radicals were found to mediate the production of H2O2 from oxygen for arsenite oxidation.In addition to the H2O2-weathered PFs,the ozone-aged PF and polystyrene were also found to have electron-donating and arsenite-oxidation capacity.This study reports important redox properties of microplastics and their effect in mediating contaminant transformation.These findings will help to better understand the fate,transformation,and biogeochemical roles of microplastics on element cycling and contaminant fate. | Lin Chen Dengjun Wang Tianran Sun Tingting Fan Song Wu Guodong Fang Min Yang Dongmei Zhou | 2023 | Fundamental Research2023，3，5： | 0 |
| 5 | Cloning and Sequence Analysis of IGFBP-1 Gene in Sheep显示文摘In this study,using Liangshan semi-fine wool sheep as an experimental material,full-length CDS sequence of IGFBP-1 gene was cloned with RT-PCR method and analyzed with bioinformatics methods. The results showed that the full-length CDS sequence of IGFBP-1 gene in Liangshan semi-fine wool sheep was792 bp in length,encoding 263 amino acids. The CDS sequence shared 97%,76% and 74% homology with bovine,human and rat,respectively; the amino acid sequence shared 97%,69% and 71%,respectively. The Gen Bank accession number was FJ589639. 1. The amino acid molecular weight of IGFBP-1 was 27. 8k D,and the theoretical isoelectric point( p I) was 5. 99. The result of phylogenetic analysis showed that IGFBP-1 gene in Liangshan semi-fine wool sheep exhibited close phylogenetic relationships with bovine,goat and other mammals,and distant phylogenetic relationships with chicken and fish. IGFBP-1 gene had obvious hydrophobic and hydrophilic regions,harboring one signal peptide,one transmembrane region,16 phosphorylation sites,six N-glycosylation sites and eight O-glycosylation sites. The result of secondary structure analysis showed that the random coil,α-helix and β-sheet regions accounted for 61. 98%,24. 33% and 13. 69%,respectively. The result of tertiary structure analysis showed that IGFBP-1 harbors an IGFBP_N domain and a thyroglobulin type-I domain. This study laid a solid foundation for further investigating the function of IGFBP-1 gene in sheep. | Mingliang ZHOU Pinggui YANG Dengjun WU Xiangyu ZHANG | 2015 | Agricultural Biotechnology2015，4，5： | 0 |
| 6 | Cloning and Sequence Analysis of IGFBP-7 Gene in Sheep显示文摘In this study,full-length CDS sequence of IGFBP-7 gene was cloned from Liangshan semi-fine wool sheep with RT-PCR method and analyzed with bioinformatics methods. The results showed that the full-length CDS sequence of IGFBP-7 gene in Liangshan semi-fine wool sheep was 846 bp in length,encoding282 amino acids. The CDS sequence shared 99%,95% and 90% homology with bovine,human and rat,respectively; the amino acid sequence shared 98%,93%and 89%,respectively. The GenB ank accession number was FJ589640. 1. The amino acid molecular weight of IGFBP-7 was 29. 0 kD,and the theoretical isoelectric point( pI) was 8. 25. The result of phylogenetic analysis showed that IGFBP-7 gene in Liangshan semi-fine wool sheep exhibited close phylogenetic relationships with bovine,goat and other mammals,and distant phylogenetic relationships with Danio rerio and Haliotis diversicolor. IGFBP-7 gene had uniformly distributed hydrophobic and hydrophilic regions,harboring one signal peptide,two transmembrane regions,16 phosphorylation sites,four N-glycosylation sites and one O-glycosylation site. The result of secondary structure analysis showed that the random coil,α-helix and β-sheet regions accounted for 64. 89%,19. 86% and 15. 25%,respectively. The result of tertiary structure analysis showed that IGFBP-7 harbors an IGFBP_N domain and an Ig-like domain. This study provided scientific basis for further investigating the function of IGFBP-7 gene in sheep. | Mingliang ZHOU Pinggui YANG Dengjun WU Xiangyu ZHANG | 2015 | Agricultural Biotechnology2015，4，6： | 0 |
| 7 | Expression Pattern of IGF-I Gene and Correlation between Its SNPs and Growth Traits in Sheep显示文摘This study was to investigate the expression pattern of IGF-I gene and correlation between its SNPs and growth traits in sheep.The expression patterns of IGF-I gene in brain,muscle,skin,liver and heart of Liangshan semi-wool sheep were detected at six growth stages(15,60,105,195,240 days) by employing qRT-PCR approach,and the correlation between SNPs of IGF-I gene and growth traits via SSCP method.The first inflection point of IGF-I gene expression was observed at day 105 in all five tissues.From 15-105 days IGF-I gene gave a decline-ascending trend in muscle,brain and liver,while it did not vary significantly during day 15-day 60 and was up-regulated in heart and liver at day 105.Whenafter,the expression level was down-regulated in liver,while that in muscle,heart,brain and skin assumed the second inflection point.The expression levels of IGF-I gene in brain and skin at day 195 were significantly higher than that at day 150 and day 240.These suggest the synchronous expression of IGF-I gene in all five tissues.Two SNPs located at the leader region and exon 3 of IGF-I gene were middle or low polymorphic.The SNP locus detected with primer P-1 was significantly correlated with birth weight of Liangshan semi-wool sheep(P <0.05),while that detected with primer P-2 was significantly correlated with weaning weight and the weaning daily gain(P < 0.05).The expression of IGF-I in all tissues had the similar developmental patterns,and strong correlation of the SNPs of IGF-I gene was confirmed with the eary growth traits to provide theoretical basis for the growth regulation and applying to assisted selective breeding with the SNPs in liangshan semi-wool sheep. | Zhou Mingliang Zhang Xiangyu Qi Songzhi Wang Qi Wu Dengjun Yang Pinggui | 2015 | Animal Husbandry and Feed Science2015，7，1： | 0 |
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