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| 1 | Antimicrobial activity,cytotoxicity,and phytochemical screening of Voacanga globosa(Blanco) Merr.leaf extract(Apocynaceae)显示文摘Objective:To determine the antibacterial,antifungal,antiprotozoal,cytotoxic,and phytochemical properties of ethanol extracts of leaves of Voacanga globosa(Blanco) Merr.(V.globosa). Methods:The extracts were tested against bacteria and fungus through disc diffusion assay; against protozoa through growth curve determination,antiprotozoal and cytotoxicity assays. Results:The extract revealed antibacterial activities,inhibiting the growth of Staphylococcus aureus,Bacillus cereus,Pseudomonas aeruginosa,Micrococcus luteus,and Salmonella typhimurium.Antifungal assay showed that it inhibited Candida albicans.The antiprotozoal assay against Trichomonas vaginalis and Entamoeba histolytica showed that V.globosa can inhibit the parasites,wherein the action can be comparable to metronidazole.With the in situ cell death detection kit.Trichomonas vaginalis and Entamoeba histolytica exposed to V.globosa leaf extract was observed to fluoresce simultaneously in red and yellow signals signifying apoptotic-like changes.Preliminary phytochemical screening revealed the chemical composition of plant extract containing alkaloids,saponins,2-deoxysugars,and hydrolysabie tannins.Conclusions: Thus,thus study provides scientific evidence on the traditional use of V.globosa leaf extract in treating microbial diseases.Further,the leaf extract can possibly be used to produce alternative forms of antimicrobials. | Pierangeli G Vital Windell L Rivera | 2011 | Asian Pacific Journal of Tropical Medicine2011，4，10： | 5 |
| 2 | Comparison of direct fecal smear microscopy,culture,and polymerase chain reaction for the detection of Blastocystis sp.in human stool samples显示文摘Objective:To compare the sensitivity and specificity of direct fecal smear microscopy,culture,and polymerase chain reaction in the detection of Blastocystis sp.in human stool.Methods:Human stool samples were collected from a community in San Isidro,Rodriguez,Rizal,Philippines.These samples were subjected to direct fecal smear microscopy,culture and polymerase chain reaction to detect the presence of Blastocystis sp.Results:Of the 110 stool samples collected,28(25%)were detected positive for the presence of Blastocystis sp.by two or more tests.Culture method detected the highest number of Blastocystis-positive stool samples(n=36),followed by PCR of DNA extracted from culture(n=26),PCR of DNA extracted from stool(n=10),and direct fecal smear(n=9).Compared to culture,the sensitivity of the other detection methods were 66.7%for PCR from culture and 19.4%for both PCR from stool and direct fecal smear.Specificity of the methods was high,with PCR from culture and direct fecal smear having97.3%,while PCR from stool at 95.9%.Conclusions:In this study,in vitro culture is the best method for detecting Blastocystis sp.in human stool samples. | Herbert J Santos Windell L Rivera | 2013 | Asian Pacific Journal of Tropical Medicine2013，6，10： | 3 |
| 3 | Development of loop-mediated isothermal amplification for rapid detection of Entamoeba histolytica显示文摘Objective:To develop a loop-mediated isothermal amplification(LAMP) assay for the detection of Entamoeba histolytica(E.histolytica),the causative agent of amebiasis.Methods:The LAMP primer set was designed from E.histolytica hemolysin gene HLY6.Genomic DNA of E.histolytica trophozoites strain HK9 was used to optimize the LAMP mixture and conditions.Amplification of DNA in the LAMP mixture was monitored through visual inspection for turbidity of the LAMP mix as well as addition of fluorescent dye.Results:Positive LAMP reactions turned turbid while negative ones remained clear.Upon addition of a fluorescent dye,all positive reactions turned green while the negative control remained orange under ambient light After elecrophoresis in 1.5% agarose gels,a ladder of multiple bands of different sizes can be oliserved in positive samples while no bands were detected in the negative control.The sensitivity of the assay was found to be S parasites per reaction which corresponds to approximately 1S.8 ng/μL DNA.The specificity of the assay was verified by the absence of amplified products when DNA from other gastrointestinal parasites such as the morphologically similar but non-pathogenic species,Entamoeba dispar. and other diarrhea-causing organisms such as Blastocystis hominis and Escherichia coli were used.Conclusions:The I.AMP assay we have developed enables the detection of E.histolytica with rapidity and ease,therefore rendering it is suitable for laboratory and field diagnosis of amebiasis. | Windell L.Rivera Vanissa A.Ong | 2013 | Asian Pacific Journal of Tropical Medicine2013，6，6： | 2 |
| 4 | Pillidal Origin of GABA Release within the Substantia Nigra Pars Reticulata during High-Frequency Stimulation of the Subthalamic Nucleus显示文摘 | Windels F Carcenac C Poupard A | 2005 | J Neurosci2005，25，20： | 1 |
| 5 | Event - specific plasmid standards and real -time PCR methods for transgenic Bt11,Bt176,and GK21 Maize and transgenic GT73 canola 显示文摘 | Taverniers I Windels P Vaitilingom M | 2005 | J Agric Food Chem2005，53，： | 1 |
| 6 | Visible continuum generation in air-silica microstructure optical fibers with anomalous dispersion at 800 nm显示文摘 | J K Ranka R S Windeler A J Stentz | 2000 | Opt Lett2000，25，1： | 1 |
| 7 | Event- speeifie plasmid standards and real -time PCR methods for transgenie Btll, Bt176 and GA21 Maize and transgenie GT73 eanola 显示文摘 | Tavemiers I Windels P Vaitilingom M | 2005 | Agrie Food Chem2005，53，： | 1 |
| 8 | 显示文摘 | Pieter Windels Isabel Taverniers Ann Depicker Erik Van Bockstaele Marc De Loose | 2001 | European Food Research and Technology2001，，2： | 1 |
| 9 | Organizing and evaluating interfirm networks: A structurationist perspective on network processes and effectiveness 显示文摘 | Sydow J Windeler A | 1998 | Organization Science1998，9，3： | 1 |
| 10 | Visible continuum generation in air-silica microstructure optical fibers with anomalous dispersion at 800 nm 显示文摘 | Ranka J K Windeler R S Stentz A J | 2003 | Opt Lett2003，25，1： | 1 |
| 11 | Low-loss hollow- core fibers with improved single-modedness显示文摘 | J M Fini J W Nicholson R S Windeler | 2013 | Opt Express2013，21，5： | 1 |
| 12 | Fibrinogen concentrates for bleeding trauma patients: what is the evidence? 显示文摘 | Meyer MAS Ostrowski SR Windel NA | 2011 | Vox Sang2011，101，3： | 1 |
| 13 | An investigation of dental luting eementsolubility as a function of the marginal gap显示文摘 | JacobsMS Windeler AS | 1991 | J Prosthet Dent1991，65，3： | 1 |
| 14 | Visible continuum generation in air-silica microstructure optical fibers with anomalous dispersion at 800 nm显示文摘 | Ranka J K Windeler R S Stentz A J | 2000 | Opt Lett2000，25，： | 1 |
| 15 | Optically driven deposition of single-walled carbon-nanotube saturable absorbers on opticalfiber end-faces显示文摘 | Nicholson J W Windeler R S DiGiovanii D J | 2007 | Opt Express2007，15，15： | 1 |
| 16 | Transcatheter Embolization of Hypogastric Artery Aneurysms: Lessons Learned显示文摘 | Lorraine M. Wolpert MD A. David. Drezner MD PhD Michael J. Hallisey MD James J. Gallagher MD Mary H. Windels MD | 2004 | Annals of Vascular Surgery2004，，4： | 1 |
| 17 | Economic and social im- pacts of Fusarium head blight: Chang- ing farm and rural communities in the Northern Great Plains显示文摘 | WINDELS CE | 2000 | Phytopatholo- gy2000，90，： | 1 |
| 18 | Event-specific plasmid standard and real-time PCR methods for transgenic Bt11,Bt176,and GA21 maize and transgenic GT73canola显示文摘 | TAVERNIERS I WINDELS P VAITILINGOM M | 2005 | Journal of Agricultural and Food Chemistry2005，53，： | 1 |
| 19 | Specificity of motor components in the dual flagellar system of Shewanella putrefaciens CN-32显示文摘 | Bubendorfer S Held S Windel N | | 0，，02： | 1 |
| 20 | Visible Continuum Generation in Air-silica Microstructure Optical Fibers with Anomalous Dispersion at 800 nm 显示文摘 | RANDA J K WINDELER R S RUSELL P SJ | 2000 | Opt Lett2000，25，： | 1 |
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