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| 1 | Effect of acupotomy on chondrocyte proliferation and expression of CyclinD1,CDK4 and CDK6 in rabbits with knee osteoarthritis显示文摘Objective:The aim of this study was to investigate the mechanism of acupotomology(Apo)in the prevention of articular cartilage destruction via the promotion of chondrocyte proliferation and chondrocyte expression of cell cycle regulators,CyclinD1,CDK4 and CDK6 in a rabbit knee osteoarthritis(KOA)model.Methods:Twenty-eight rabbits were randomly divided into a control group,an OA(osteoarthritis)model group,an Apo(acupotomology)group and EA(electro-acupuncture)group(n Z 7).Improved Videman’s method was used to induce a rabbit model of KOA over 6 weeks.One week later,acupotomy and electro-acupuncture therapy was applied to animals in the respective groups and treatment lasted 4 weeks.Following these treatments,quantitative real-time PCR,immunohistochemical staining and western blotting were performed to assess the mRNA and protein levels of cell cycle regulators CyclinD1(Cell cycle protein D1),CDK4(Cyclin-dependent kinase 4)and CDK6(Cyclin-dependent kinase 6).Ethology measures and knee morphology were also compared among groups.Results:The Lequesne MG index score of morphology was increased(P<.01),and the passive range of motion(PROM)and the mRNA and protein levels of CyclinD1,CDK4,and CDK6 were significantly decreased(P<.01)in the OA model compared with the control group.The Lequesne MG index score and the morphology score were decreased in the Apo and EA group compared with the OA model group(P<.05 or P<.01),while the mRNA levels of CyclinD1,CDK4,and CDK6,and the protein levels of CDK4 were increased in the Apo and EA groups compared with the OA model group(P<.05 or P<.01).The PROM,and the protein levels of CyclinD1 and CDK6 were increased(P<.05)in the Apo group compared with the OA model group,while the PROM and the protein levels of CyclinD1 and CDK6 in the EA group were not significantly different(P>.05).Compared with the EA group,the morphology score was decreased in Apo group(P<.05).Conclusions:The mRNA levels of CyclinD1 and CDK4,and the protein level of CDK4 in chondrocytes are regulate by both Apo and EA.Apo is more effective than EA in regulating the protein levels of CyclinD1 and CDK6.According to the observed changes in morphology and cytokine levels,acupotomy can promote chondrocyte proliferation and can alleviate the destruction of articular cartilage in a model of KOA. | Yang Gao Tong Wang Wei Zhang Xiaowei Shi Shining Ma Lijuan Wang Shuai Zhou Defeng Wang Meyer Magali Changqing Guo | 2019 | Journal of Traditional Chinese Medical Sciences2019，6，3： | 2 |
| 2 | Spread of disease via the subperitoneal space:the small bowel mesentery显示文摘 | Berne AS Meyers MA | 1993 | Abdom Imaging1993，18，2： | 1 |
| 3 | Prevalence of benign disease in patients undergoing resection for suspected lung cancer显示文摘 | Smith MA Battafarano RJ Meyers BF | 2006 | Ann Thorac Surg2006，81，5： | 1 |
| 4 | Neuropsychological effects of third ventricle tumor surgery显示文摘 | Friedman MA Meyers CA Sawaya R | 2008 | Neurosurgery2008，52，4： | 1 |
| 5 | A Global approach to childhood lead poisoning prevention 显示文摘 | Meyer PA McGeehin MA Falk H | 2003 | Int J Hyj Environ Health2003，206，： | 1 |
| 6 | Casecrossover and case-time-control designs in birth defects epidemiology显示文摘 | Hernandez-Diaz S Hernan MA Meyer K | 2003 | Am J Epidemiol2003，158，4： | 1 |
| 7 | The peritoneal ligments and mesenteries:pathways of intraabdominal spread of disease显示文摘 | Meyers MA Oliphant M Berne AS | 1987 | Radioloy1987，163，： | 1 |
| 8 | Global disparities in the epilepsy treatment gap: a systematic review 显示文摘 | Meyer AC Dua T Ma J | 2010 | Bull World Health Organ2010，88，4： | 1 |
| 9 | High - dose steroids, ursodeoxycholic acid, and chronic intravenous anti- biotics improve bile flow after Kasaiprocedure in infants with biliary atresia 显示文摘 | Meyers RL Book LS O' Gorman MA | 2003 | J Pediatr Surg2003，38，3： | 1 |
| 10 | Haustral anatomy and pathology:a new look-roentgen interpretation of pathological alterations显示文摘 | Meyers MA Volberg F Katzen B | 1973 | Radiology1973，108，： | 1 |
| 11 | Prevention of ocular scarring after glaucoma filtering surgery using the monoclonal antibody LTI009 (Sonepcizumab) in a rabbit model显示文摘 | Lukowski ZL Min J Beattie AR Meyers CA Levine MA Stoller G | 2013 | J Glaucoma2013，22，2： | 1 |
| 12 | The effect of phacoemulsifica-tion on aqueous out flow facility显示文摘 | Meyer MA Savitt ML Kopitas E | 1997 | Ophthalmology1997，104，8： | 1 |
| 13 | alpha7 nicotinic receptor-mediated protection against ethanol-induced oxidative stress and cytotoxicity in PC12 cells显示文摘 | Li Y King MA and Meyer EM | 2000 | Brain Res2000，861，： | 1 |
| 14 | Neuropsychological effects of third ventricle tumor surgery 显示文摘 | Friedman MA Meyers CA Sawaya R | 2008 | Neurosurgery2008，62，63： | 1 |
| 15 | Radiological features of spread and localization of extraperitoneal gas and their relationship to its source显示文摘 | Meyers MA | 1974 | Diag Radiol1974，111，1： | 1 |
| 16 | Normalized metal artifact reduction in head and neck computed tomography显示文摘 | Lell MM Meyer E Kuefner MA | 2012 | Invest Radiol2012，47，7： | 1 |
| 17 | Comparison of perfusion lung scanning and angiography in the estimation of vascular obstruction in acute pulmonary embolism显示文摘 | Meyer G Collignon MA Guinet F | | 0，，6： | 1 |
| 18 | High-dose steroids, ursodeoxycholic acid, and chronic intravenous antibiotics improve bile flow after Kasai procedure in infants with biliary atresia 显示文摘 | Meyers RL Book LS O'Gorman MA | 2003 | J Pediatr Surg2003，38，3： | 1 |
| 19 | Carbamazepine and serum sodium levels显示文摘 | Kalff R Houtkooper MA Meyer JW | 1984 | Epilepsia1984，25，3： | 1 |
| 20 | Imaging the direct bidirectional spread of disease between the abdomen and the female pelvis via the subperitoneal space显示文摘 | Oliphant M Berne AS Meyers MA | 1988 | Gastrointest Radiol1988，13，： | 1 |
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