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| 1 | Detection of disseminated pancreatic cells by amplification of cytokeratin-19 with quantitative RT-PCR in blood,bone marrow and peritoneal lavage of pancreatic carcinoma patients显示文摘AIM: To evaluate the diagnostic potential of cytokeratin-19 (CK-19) mRNA for the detection of disseminated tumor cells in blood, bone marrow and peritoneal lavage in patients with ductal adenocarcinoma of the pancreas. METHODS: Sixty-eight patients with pancreatic cancer (n = 37), chronic pancreatitis (n = 16), and non-pan- creatic benign surgical diseases (n = 15, control group) were included in the study. Venous blood was taken preoperatively, intraoperatively and at postoperative d 1 and 10. Preoperative bone marrow aspirates and peritoneal lavage taken before mobilization of the tumor were analyzed. All samples were evaluated for disseminated tumor cells by CK-19-specific nested-PCR and quantitative fluorogenic RT-PCR. RESULTS: CK-19 mRNA expression was increased in 24 (64%) blood samples and 11 (30%) of the peritoneal lavage samples in the patients with pancreatic cancer. In 15 (40%) of the patients with pancreatic cancer, disseminated tumor cells were detected in venous blood and bone marrow and/or peritoneal lavage. In the peritoneal lavage, the detection rates were correlated with the tumor size and the tumor differentiation. CK-19 levels were increased in pT3/T4 and moderately/poorly differentiated tumors (G2/G3). Pancreatic cancer patients with at least one CK-19 mRNA-positive sample showed a trend towards shorter survival. Pancreatic cancerpatients showed significantly increased detection rates of disseminated tumor cells in blood and peritoneal lavage compared to the controls and the patients with chronic pancreatitis. CONCLUSION: Disseminated tumor cells can be detected in patients with pancreatic ductal adenocar- cinoma by CK-19 fluorogenic RT-PCR. In peritoneal lavage, detection rate is correlated with tumor stage and differentiation. In the clinical use, CK-19 is suitable for the distinction between malignant and benign pancreatic disease in combination with other tumor-specific markers. | Katrin Hoffmann Christiane Kerner Wolfgang Wilfert Marc Mueller Joachim Thiery Johann Hauss Helmut Witzigmann | 2007 | World Journal of Gastroenterology2007，13，2： | 20 |
| 2 | Irradiation Induces a Biphasic Expression of Pro-Inflammatory Cytokines in the Lung显示文摘 | Claudia E. Rübe MD Falk Wilfert Jan Palm Jochem K?nig Susanne Burdak-Rothkamm Li Liu Andreas Schuck Normann Willich Christian Rübe | 2004 | Strahlentherapie und Onkologie2004，，7： | 2 |
| 3 | Design of inter-area oscillation damping controllers using global measurements显示文摘 | KüBner K Erlich I Wilfert H H | 2001 | IEEE Proto Power Tech Conference 10^th-13^th Porto Portugal2001，2001，： | 1 |
| 4 | Irradiation induces a biphasicexpression of pro-inflammatory cytokines in the lung 显示文摘 | Riibe CE Wilfert F Palm J | 2004 | Strahlenthe Onkol2004，180，7： | 1 |
| 5 | Branched polyphosphazenes with controlled dimensions 显示文摘 | Henke H Wilfert S lturmendi A | 2013 | Journal of Polymer Science Part A: Polymer Chemistry2013，51，20： | 1 |
| 6 | Miniaturized Vacuum Gauges显示文摘 | Wilfert St | 2004 | J Vac Sci Technol A2004，22，2： | 1 |
| 7 | The bronehiolar epithelium as a prominent source of pro-inflammatory eytoklnes after lung irradiation 显示文摘 | Rube C E Uthe D Wilfert F | 2005 | Int J Radiat Oncol 8iol Phys2005，61，5： | 1 |
| 8 | Experimental bridging of osseous defects in rats by the implantation of Kiel bone containing fresh autologous marrow显示文摘 | Plenk H Hollmann K Wilfert K-H | 1972 | J Bone Joint Surg1972，54，4： | 1 |
| 9 | Complex model of terrestrial FSO links显示文摘 | Kolka Z Wilfert O Kvicala R | 2007 | SPIE2007，6709，： | 1 |
| 10 | An introduction to free-space optical communications显示文摘 | HENNIGER H WILFERT O | 2010 | Radioengineering2010，19，2： | 1 |
| 11 | Entraviruses and meningitis显示文摘 | Wilfert CM Cehrman SM Katz SL | 1983 | J Pediatr In Fect Dis1983，4，2： | 1 |
| 12 | Comparison of TLD calibration methods for 192Ir dosimetry显示文摘 | HAWORTH A BUTLER D J WILFERT L | 2013 | J Appl Clin Med Phys2013，14，1： | 1 |
| 13 | An introduction to free-space optical communications显示文摘 | Hennes Henniger Otakar Wilfert | 2010 | Radio Enginnering2010，19，2： | 1 |
| 14 | Irradiation induces a biphasic expression of pro - inflammatory cytokines in the lung 显示文摘 | Rube CE Wilfert F Plam J | 2004 | Strhlenther Onkol2004，180，： | 1 |
| 15 | Miniaturized vacuum gauges显示文摘 | Edelmann C | 2004 | Journal of Vacuum Science Technology A2004，22，2： | 1 |
| 16 | The bronchiolar epitheli- um as a prominent source of pro-inflammatory cytokines after lung irradiation显示文摘 | Rube CE Uthe D Wilfert F | 2005 | Int J Radiat Oncol Biol Phys2005，61，5： | 1 |
| 17 | An introduction to free-space optical communications显示文摘 | Henniger H Wilfert O | 2010 | Radioengineering2010，19，2： | 1 |
| 18 | The bronchiolar epithelium as a prominent source of pro-inflammatory eytokines after lung irradiation Int显示文摘 | Rube CE Uthe D Wilfert F | 2005 | J Radiation Oneology Biol Phys2005，61，： | 1 |
| 19 | Irradiation induces a biphasic expression of pro-inflammatory cytokines in the lung显示文摘 | Rube C E Wilfert F Palm J | 2004 | Strahlenther Onko12004，180，7： | 1 |
| 20 | Modulation of radiationinduced tumour necrosis factor a(TNF-a)expression in the lung tissue by pentoxifylline显示文摘 | Rube C E Wilfert F Uthe D | 2002 | Radiotherapy and oncology2002，64，2： | 1 |
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