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| 1 | A high-quality genome sequence of alkaligrass provides insights into halophyte stress tolerance显示文摘Alkaligrass(Puccinellia tenuiflora) is a monocotyledonous halophytic forage grass widely distributed in Northern China. It belongs to the Gramineae family and shares a close phylogenetic relationship with the cereal crops, wheat and barley. Here, we present a high-quality chromosome-level genome sequence of alkaligrass assembled from Illumina, Pac Bio and 10× Genomics reads combined with genome-wide chromosome conformation capture(Hi-C) data. The ~1.50 Gb assembled alkaligrass genome encodes 38,387 protein-coding genes, and 54.9% of the assembly are transposable elements, with long terminal repeats being the most abundant. Comparative genomic analysis coupled with stress-treated transcriptome profiling uncovers a set of unique saline-and alkaline-responsive genes in alkaligrass. The high-quality genome assembly and the identified stress related genes in alkaligrass provide an important resource for evolutionary genomic studies in Gramineae and facilitate further understanding of molecular mechanisms underlying stress tolerance in monocotyledonous halophytes. The alkaligrass genome data is freely available at http://gffzz284c18d2de364501hkxpwoxpqf0qv6owv.ffgz.tsg.suse.edu.cn/data/alkaligrass.html. | Wenting Zhang Jie Liu Yongxue Zhang Jie Qiu Ying Li Baojiang Zheng Fenhong Hu Shaojun Dai Xuehui Huang | 2020 | Science China(Life Sciences)2020，63，9： | 8 |
| 2 | Evaluating Common Land Model Energy Fluxes Using FLUXNET Data显示文摘Given the crucial role of land surface processes in global and regional climates, there is a pressing need to test and verify the performance of land surface models via comparisons to observations. In this study, the eddy covariance measurements from 20 FLUXNET sites spanning more than 100 site-years were utilized to evaluate the performance of the Common Land Model(Co LM) over different vegetation types in various climate zones. A decomposition method was employed to separate both the observed and simulated energy fluxes, i.e., the sensible heat flux, latent heat flux, net radiation, and ground heat flux, at three timescales ranging from stepwise(30 min) to monthly. A comparison between the simulations and observations indicated that Co LM produced satisfactory simulations of all four energy fluxes, although the different indexes did not exhibit consistent results among the different fluxes. A strong agreement between the simulations and observations was found for the seasonal cycles at the 20 sites, whereas Co LM underestimated the latent heat flux at the sites with distinct dry and wet seasons, which might be associated with its weakness in simulating soil water during the dry season. Co LM cannot explicitly simulate the midday depression of leaf gas exchange, which may explain why Co LM also has a maximum diurnal bias at noon in the summer. Of the eight selected vegetation types analyzed, Co LM performs best for evergreen broadleaf forests and worst for croplands and wetlands. | Xiangxiang ZHANG Yongjiu DAI Hongzhi CUI Robert E.DICKINSON Siguang ZHU Nan WEI Binyan YAN Hua YUAN Wei SHANGGUAN Lili WANG Wenting FU | 2017 | Advances in Atmospheric Sciences2017，34，9： | 4 |
| 3 | Profiles and Source Apportionment of Nonmethane Volatile Organic Compounds in Winter and Summer in Xi’an, China, based on the Hybrid Environmental Receptor Model显示文摘Summer and winter campaigns for the chemical compositions and sources of nonmethane hydrocarbons(NMHCs)and oxygenated volatile organic compounds(OVOCs)were conducted in Xi’an.Data from 57 photochemical assessment monitoring stations for NMHCs and 20 OVOC species were analyzed.Significant seasonal differences were noted for total VOC(TVOC,NMHCs and OVOCs)concentrations and compositions.The campaign-average TVOC concentrations in winter(85.3±60.6 ppbv)were almost twice those in summer(47.2±31.6 ppbv).Alkanes and OVOCs were the most abundant category in winter and summer,respectively.NMHCs,but not OVOCs,had significantly higher levels on weekends than on weekdays.Total ozone formation potential was higher in summer than in winter(by 50%)because of the high concentrations of alkenes(particularly isoprene),high temperature,and high solar radiation levels in summer.The Hybrid Environmental Receptor Model(HERM)was used to conduct source apportionment for atmospheric TVOCs in winter and summer,with excellent accuracy.HERM demonstrated its suitability in a situation where only partial source profile data were available.The HERM results indicated significantly different seasonal source contributions to TVOCs in Xi’an.In particular,coal and biomass burning had contributions greater than half in winter(53.4%),whereas traffic sources were prevalent in summer(53.1%).This study’s results highlight the need for targeted and adjustable VOC control measures that account for seasonal differences in Xi’an;such measures should target not only the severe problem with VOC pollution but also the problem of consequent secondary pollution(e.g.,from ozone and secondary organic aerosols). | Jian SUN Zhenxing SHEN Yue ZHANG Wenting DAI Kun HE Hongmei XU Zhou ZHANG Long CUI Xuxiang LI Yu HUANG Junji CAO | 2021 | Advances in Atmospheric Sciences2021，38，1： | 2 |
| 4 | Association between TSHR gene polymorphism and the risk of Graves' disease:a meta-analysis显示文摘Thyroid stimulating hormone receptor(TSHR) is thought to be a significant candidate for genetic susceptibility to Graves' disease(GD).However,the association between TSHR gene polymorphism and the risk of GD remains controversial.In this study,we investigated the relationship between the two conditions by meta-analysis.We searched all relevant case-control studies in PubMed,Web of Science,CNKI and Wanfang for literature available until May2015,and chose studies on two single nucleotide polymorphisms(SNPs):rs 179247 and rsl2101255,within TSHR intron-1.Bias of heterogeneity test among studies was determined by the fixed or random effect pooled measure,and publication bias was examined by modified Begg's and Egger's test.Eight eligible studies with 15 outcomes were involved in this meta-analysis,including 6,976 GD cases and 7,089 controls from China,Japan,Poland,UK and Brazil.Pooled odds ratios(ORs) for allelic comparisons showed that both TSHR rsl79247A/G and rsl2101255T/C polymorphism had significant association with GD(OR=1.422,95%CI=1.353—1.495,P<0.001,P_(heterogeneity)=0.448;OR= 1.502,95%CI:1.410-1.600,P<0.001,P_(heterogeneity)=0.642),and the associations were the same under dominant,recessive and co-dominant models.In subgroup analyses,the conclusions are also consistent with all those in Asian,European and South America subgroups(P<0.001).Our meta-analysis revealed a significant association between TSHR rsl79247A/G and rsl2101255T/C polymorphism with GD in five different populations from Asia,Europe and South America.Further studies are needed in other ethnic backgrounds to independently confirm our findings. | Wei Qian Kuanfeng Xu Wenting Jia Ling Lan Xuqin Zheng Xueyang Yang Dai Cui | 2016 | The Journal of Biomedical Research2016，30，6： | 2 |
| 5 | Bioinformatics Analysis on Pathogenic Genes of Botrytis cinerea Pers.显示文摘With reported pathogenic genes of Botrytis cinerea Pers. and unknown proteins related to pathogencity as experimental materials,the pathogenic genes were studied by bioinformatics methods,and these unknown proteins were analyzed with bioinformatics tools and softwares,so as to lay a theoretical basis for the control of tomato grey mould at molecular level in future. The results showed that through the alignment analysis by DNAMAN software,three pairs of unknown proteins with higher comprehensive matching degrees were obtained,and these proteins were predicted to be coded by hrp gene cluster of type III secretion system;through Bioedit and Prot Param,the amino acid composition,size,molecular weight,isoelectric point and hydrophobicity of the each of the six unknown proteins were obtained; and corresponding secondary structures were obtained using DNAMAN. The results of this study could guide deep research of such genes. | Qiang ZHANG Wenting DAI Xinwen JIN Xuhui WANG | 2018 | Agricultural Biotechnology2018，7，1： | 2 |
| 6 | Control of membrane fouling during hyperhaline municipal wastewater treatment using a pilot-scale anoxic/aerobic-membrane bioreactor system显示文摘Membrane fouling limits the effects of long-term stable operation of membrane bioreactor (MBR).Control of membrane fouling can extend the membrane life and reduce water treatment cost effectively.A pilot scale anoxic/aerobic-membrane bioreactor (A/OMBR,40 L/hr) was used to treat the hyperhaline municipal sewage from a processing zone of Tianjin,China.Impact factors including mixed liquid sludge suspension (MLSS),sludge viscosity (μ),microorganisms,extracellular polymeric substances (EPS),aeration intensity and suction/suspended time on membrane fouling and pollution control were studied.The relationships among various factors associated with membrane fouling were analyzed.Results showed that there was a positive correlation among MLSS,sludge viscosity and trans-membrane pressure (TMP).Considering water treatment efficiency and stable operation of the membrane module,MLSS of 5 g/L was suggested for the process.There was a same trend among EPS,sludge viscosity and TMP.Numbers and species of microorganisms affected membrane fouling.Either too high or too low aeration intensity was not conducive to membrane fouling control.Aeration intensity of 1.0 m3 /hr (gas/water ratio of 25:1) is suggested for the process.A long suction time caused a rapid increase in membrane resistance.However,long suspended time cannot prevent the increase of membrane resistance effectively even though a suspended time was necessary for scale off particles from the membrane surface.The suction/suspended time of 12 min/3 min was selected for the process.The interaction of various environmental factors and operation conditions must be considered synthetically. | Jingmei Sun Jiangxiu Rong Lifeng Dai Baoshan Liu Wenting Zhu | 2011 | Journal of Environmental Sciences2011，23，10： | 1 |
| 7 | Optimization of heat shield for single silicon crystal growth by using numerical simulation显示文摘In integrated circuit-grade single silicon Czochralski growth, the position and material of heat shield are main parameters affecting the heat exchange and crystal growth condition. By optimizing the above parameters, we attempted to increase the growth rate and crystal quality. Numerical simulation proved to verify the results before and after optimization. Through analyses of the temperature and microdefect distribution, it is found that the optimized heat shield can further increase the pulling rate and decrease the melt/crystal interface deflection, increase the average velocity of argon flow from ~2 to ~5 m·s^-1 , which is in favor of the transportation of SiO, and obtain the low defects concentration crystal and that the average temperature along the melt-free surface is 8 °C higher than before avoiding supercooled melt effectively. | TENG Ran ZHOU Qigang DAI Xiaolin WU Zhiqiang XU Wenting XIAO Qinghua WU Xiao GUO Xi | 2012 | Rare Metals2012，31，5： | 1 |
| 8 | Numerical simulation of effects of cusp magnetic field on oxygen concentration of 300 mm CZ-Si显示文摘In magnetic Czochralski (MCZ) silicon growth, the distance and diameter of the electrified coils may affect the magnetic field intensity and melt flow. By changing the above parameters, the optimum geometric configuration of the coils was attempted. Through analyses of the oxygen concentration distribution of the crystal/melt interface, axial and radial velocity distribution of melt and the magnetic field intensity in the melt, it is found that smaller diameter of coils contributes to reducing the needed current intensity and production costs. For a given current intensity, there is a best distance of coils when the oxygen concentration at crystal/melt interface reaches the lowest. | WANG Yongtao XU Wenting DAI Xiaolin XIAO Qinghua DENG Shujun YAN Zhirui ZHOU Qigang | 2012 | Rare Metals2012，31，5： | 1 |
| 9 | Fresh-Keeping Technology of Zander Fillet with Vacuum Packaging and Partial Freezing显示文摘In order to explore the fresh-keeping methods for zander fillet,we use vacuum packaging and partial freezing to process zander fillet,conduct a comparative analysis of sensory characteristics,percentage of water loss,stiffness period,muscle stiffness,TVB-N and water activity during the storage,and assess the fresh-keeping effect of zander fillet under the conditions of vacuum packaging and partial freezing. The results show that zander can be dead after being placed into the 2℃ water for 30 min,and the stiffness period can be extended about 100 min compared with natural death; the percentage of water loss under the conditions of vacuum packaging before partial freezing is 2. 6% lower than under the conditions of partial freezing before vacuum packaging; after being stored at- 7℃ and- 4℃ for 15 d,TVB- N of fillet ≤15 mg /kg; TVB- N of fillet at- 7℃ is 25% lower than at- 4℃. | Qiang ZHANG Wenting DAI Xinwen JIN | 2016 | Asian Agricultural Research2016，8，3： | 0 |
| 10 | Investigation of Gray Mold of Protected Tomato and Resistance Identification of Variety in Shihezi Area,Xinjiang显示文摘[Objective] The paper was to understand the occurrence of gray mold of protected tomato and resistance of introduced tomato varieties to gray mold in Shihezi area. [Method] The occurrence status of gray mold in protected tomato in Shawan County, Manas County, the experimental field of Shihezi University and Shihezi Huayu base were investigated during the occurrence period of tomato gray mold, and the resistance of intro-duced variety against tomato gray mold was identified at seedling stage through indoor artificial inoculation method. [Result] The diseased leaf rate of tomato gray mold was 17.52%-100%, the diseased fruit rate was 3.1%-55.69%, and the disease index was 15.98-100. In resistance identifica-tion of different tomato varieties, the disease indexes of Kenfan25, Shifan28, Shifan15, KENFAN-3, KENFAN-5, Shifan35, KENFAN-2 and Shifan-9 were 1.03, 3.1, 1.17, 4.3, 5.32, 5.12, 4.32 and 4.11, respectively. From September to March of the following year, tomato gray mold occurred and was prevalent in greenhouses in Shihezi area. [Conclusion] Shifan28, Kenfan25 and Shifan15 showed resistance to tomato gray mold, while the other five varieties were susceptible to the disease. | Zhang Qiang Dai Wenting Wu Hongbin Yang Hui Li Jixin | 2018 | Plant Diseases and Pests2018，9，3： | 0 |
| 11 | Preliminary Localization of Resistance Gene against Tomato Gray Mold in Xinjiang显示文摘The genetic linkage map of F1 progeny of tomato variety ' TS-107' and susceptible cultivar ' TS-100' in Xinjiang was constructed. The inheritance of resistance genes against gray mold in disease-resistant cultivar ' TS-107' was studied. Genetic mapping of resistance genes through Bulked Segregation Analysis( SSA) and Simple Sequence Recognition( SSR) markers provided germplasm resources for resistance breeding against gray mold. The results showed that the resistance of tomato ' TS-107' to gray mold was controlled by a dominant gene. Eleven SSR polymorphic markers were screened and the gene was located between LGVII markers SSR12510 and ECM123 by linkage analysis. There was a partial segregation phenomenon in the No. 327 and No. 328 markers with significant difference,and the two markers had partial segregation sites. | Zhang Qiang Dai Wenting Jin Xinwen | 2017 | Plant Diseases and Pests2017，8，6： | 0 |
| 12 | Effects of Different Parameters of Electrostatic Field on Removal of Ethylene in Fruit and Vegetable Storage Environment显示文摘[Objectives]The effects of different parameters of electrostatic field on the removal of ethylene from fruit and vegetable storage environment were studied. [Methods]The high voltage electrostatic field technology was used to simulate the removal of ethylene from fruit and vegetable storages, and its influencing factors were tested and analyzed. [Results] The removal effect of needle-plate electrodes was better than that of needle electrodes. The removal effect of ethylene at high voltage was better than that at low voltage. The length of gas chamber was positively correlated with the time required for ethylene removal. Increasing the voltage could produce more ozone, thus improving the removal effect of ethylene. [Conclusions] This study provides theoretical basis and technical support for the use of high-voltage electrostatic field to remove ethylene in controlled atmosphere storages. | Weijun CHEN Wenting DAI Zhuangsheng WU Xiaojuan WANG Hao DENG Qiang ZHANG | 2023 | Agricultural Biotechnology2023，12，1： | 0 |
| 13 | A neuropsin-based optogenetic tool for precise control of Gq signaling显示文摘G_(q)-coupled receptors regulate numerous physiological processes by activating enzymes and inducing intracellular Ca^(2+)signals.There is a strong need for an optogenetic tool that enables powerful experimental control over G_(q) signaling.Here,we present chicken opsin 5(cOpn5)as the long sought-after,single-component optogenetic tool that mediates ultra-sensitive optical control of intracellular G_(q) signaling with high temporal and spatial resolution.Expressing cOpn5 in HEK 293T cells and primary mouse astrocytes enables blue light-triggered,G_(q)-dependent Ca^(2+) release from intracellular stores and protein kinase C activation.Strong Ca^(2+) transients were evoked by brief light pulses of merely 10 ms duration and at 3 orders lower light intensity of that for common optogenetic tools.Photostimulation of cOpn5-expressing cells at the subcellular and single-cell levels generated fast intracellular Ca^(2+)transition,thus demonstrating the high spatial precision of cOpn5 optogenetics.The cOpn5-mediated optogenetics could also be applied to activate neurons and control animal behavior in a circuit-dependent manner.cOpn5 optogenetics may find broad applications in studying the mechanisms and functional relevance of G_(q) signaling in both non-excitable cells and excitable cells in all major organ systems. | Ruicheng Dai Tao Yu Danwei Weng Heng Li Yuting Cui Zhaofa Wu Qingchun Guo Haiyue Zou Wenting Wu Xinwei Gao Zhongyang Qi Yuqi Ren Shu Wang Yulong Li Minmin Luo | 2022 | Science China(Life Sciences)2022，65，7： | 0 |
| 14 | Genetic Analysis of Resistance to Tomato Gray Mold( Botrytis cinerea)显示文摘The study used four tomato varieties,03786( susceptible),05237( susceptible),C2985( resistant) and Wva536( resistant) with different resistance,to formulate combinations according to Griffing( I) complete diallel cross method. And Botrytis cinerea was inoculated to investigate the incidence of gray mold. The results showed that:(1) there were significant differences in disease resistance between different varieties and different combinations.(2) There were significant differences in general combining ability and special combining ability between the tested varieties,and Wva536 and C2985 had a higher general combining ability effect,and were both relatively good parents when formulating resistant combinations. Combinations Wva536 × 03786 and Wva536 × 05237 had the highest special combining ability,and thus have the value of further research and utilization.(3) The additive effect in resistance inheritance was located at the primary position,and partial dominance also existed as well as cytoplasmic effect.(4) The broad-sense heritability and narrow-sense heritability were,respectively,89. 73% and82. 15%,which were higher,indicating that the parents had a greater effect on offsprings. The resistance genes can be expressed in offsprings through gene accumulation,and should be selected in the early generation. | Qiang ZHANG Wenting DAI Hongbin WU Hui YANG Xinwen JIN | 2019 | Agricultural Biotechnology2019，8，1： | 0 |
| 15 | Screening of Tomato Resources Resistant to Tomato Gray Mold显示文摘This study was conducted to screen tomato resources resistant to gray mold for the first time by in-vitro stem inoculation method.The results showed that Solanum habrochaites T2-07-316 was highly resistant to gray mold,and had the relative stem infection rate and relative stem rot expansion rate of 0 on the 6^(th) day after inoculation.There were also other seven transgenic common tomato materials which showed higher resistance to gray mold,with a relative stem infection rate in the range of 15.00%-38.33% and a relative stem rot expansion rate in the range of 10.22%-23.57%.Among them,T2-07-337 had the best resistance. | Qiang ZHANG Wenting DAI Hongbin WU Hui YANG Xinwen JIN | 2018 | Agricultural Biotechnology2018，7，6： | 0 |
| 16 | SSR Molecular Marker for Late Blight Resistance Gene in Tomato CLN2037显示文摘[Objectives] The research aimed to investigate the genetic rule of the disease resistance of tomato CLN2037,find molecular markers linked with disease-resistant genes and locate the disease-resistant genes. [Methods] BCr,BCs and F_2 were constructed from tomato CLN2037 and susceptible cultivar T2-03,and their resistance to late blight was identified and analyzed by inoculating the disease in the seedling stage of tomatoes. The genetic linkage analysis was made based on the genetic linkage map constructed by ICu GI using group segregation method and 90 pairs of tomato SSR primers. [Results]The resistance of CLN2037 to late blight was controlled by a single recessive gene. The polymorphic bands amplified by primer DM0231 had a linkage relationship with resistance genes. The size of the polymorphism fragment was 226 bp with the genetic linkage distance of 2. 67 c M,and the disease resistance gene was localized on CCPB272-03741.[Conclusions]DM0231 could be used as a molecular marker for molecular breeding of tomato against late blight,which laid the foundation for further fine localization of late blight resistance genes. | Qiang ZHANG Wenting DAI Xinwen JIN Xuhui WANG | 2018 | Agricultural Biotechnology2018，7，2： | 0 |
| 17 | Exploring visitors’visual perception along the spatial sequence in temple heritage spaces by quantitative GIS methods:a case study of the Daming Temple,Yangzhou City,China显示文摘The Daming Temple,built during 457–464 C.E.,is one of the developing ancient temple heritage spaces located in Yangzhou city,P.R.China.Over the past 60 years,variation in visitors’spatial perception along the tour routes in the temple has occurred.This research attempts to reveal the changes in visitors’visual perception along the spatial sequences at 3 different times(i.e.,1962,1973 and 2022).A quantitative GIS-based method,which includes analysing the distribution of visitors’spatial preferences and spatial configuration,is proposed.Digital landscape tools and quantitative estimation methods are used,including mapping within Rhinoceros software,the kernel density estimation(KDE)method within ArcGIS software and spatial syntax analysis within DepthMap software.Extracted geodata from 500 photographs of the heritage space taken by volunteer visitors are analysed within the GIS environment.Values of the mean depth(MD)at both levels of visibility and accessibility are calculated within the visibility graph analysis(VGA)model.Comparisons between the visual preferences of the visitors and the spatial configuration along the spatial sequence are conducted.The results indicate that the spatial sequence has a significant impact on visitors’visual preferences and tour routes.The phenomenon of spatial sequence among dynamic temporal variations and the effects of narrative spaces along the spatial sequence are highlighted and explained,which reveal the relationship between visitors’geospatial preference and the spatial configuration of the temple.Some suggestions are put forwards for further studies on the revitalisation and management of East Asian ancient temple heritage spaces. | Kai Zhou Wenting Wu Tianjie Li Xiaoling Dai | 2023 | Built Heritage2023，7，4： | 0 |
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