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| 1 | Cyclophosphamide-induced reproductive toxicity: Beneficial effects of Helichrysum odoratissimum(Asteraceae) in male Wistar rats显示文摘Objective: Cyclophosphamide(CP) is commonly used to treat some cancers, but its clinical efficacy is also linked with testicular toxicity. We investigated the effects of aqueous extract(AE) and methanolic extract(ME) of Helichrysum odoratissimum for reducing CP-induced reproductive toxicity in male rats.Methods: In addition to a normal control(group 1), drugs or vehicles were administered orally to seven groups(n = 5) of rats that had already received 4-weeks of pre-treatment with CP(5 mg/[kgád], per oral administration);group 2 received CP + distilled water(10 m L/[kgád]);group 3 received CP + 5% tween 80(10 m L/[kgád]);group 4 received CP + clomiphene citrate(0.25 mg/[kgád]);groups 5 and 6 received CP + AE(50 and 100 mg/[kgád]) and groups 7 and 8 received CP + ME(50 and 100 mg/[kgád]). Animals were sacrificed on day 15, and body and sexual organ weights, sperm characteristics, testosterone level and testicular histology were evaluated.Results: The CP-treated group showed a significant reduction(P < 0.001) in the body and seminal vesicle weights, testosterone level, sperm count, sperm motility and sperm viability, but elevated(P < 0.001)sperm morphological abnormalities and testicular structure alterations, compared to the control group.Interestingly, these detrimental effects of CP were reversed by treatment with H. odoratissimum extracts.For instance, both extracts and all doses of H. odoratissimum significantly increased the sperm count(P < 0.001), sperm motility(AE, 50 mg/kg, P < 0.05;ME, 50 and 100 mg/kg, P < 0.05) and sperm viability(AE, 50 mg/kg, P < 0.001;ME, 50 and 100 mg/kg, P < 0.001) compared to the CP group. H. odoratissimum also improved plasmatic and intratesticular testosterone levels and prevented histological alterations of the testes.Conclusion: H. odoratissimum might be considered as an alternative drug to alleviate/prevent reproductive damage in cancer patients receiving CP chemotherapy. | Pierre Watcho Ismaelle Rosine Mpeck Patrick Brice Deeh Defo Modeste Wankeu-Nya Esther Ngadjui Georges Romeo Bonsou Fozin Pierre Kamtchouing Albert Kamanyi | 2019 | Journal of Integrative Medicine2019，17，5： | 3 |
| 2 | Effects of Ficus asperifolia on normal rat estrus cyclicity显示文摘Objective:To evaluate Ficus asperifolia(Moraceae)(F.asperifolia)effecting on regular estrus cycle of Wistar rats.Methods:Air-dried fruits of F.asperifolia were extracted using water.Prior to the test,vaginal smear was monitored daily for a 3-week period to select females with normal(regular)estrous cycle.Those with regular estrus cycle weighing between 150-170 g were randomized into three sets of 15 animals each.Each set was then divided into three groups:Group 1(control)was orally administered with distilled water(10 mL/kg body weight)once a day for 1 week starting from the proestrus stage.Groups 2 and 3 were respectively treated with 100and 500 mg/kg body weight of the plant aqueous extract.The two other sets of 15 animals each were similarly treated as the first set for 3 weeks and 6 weeks respectively.Estrus cycle pattern was monitored before and during plant extract application whereas lipid profile,ovary,uterus and liver growth indices were determined at the end of each treatment.Results:F.asperifolia did not disrupt(0%)the order of appearance of normal estrus cycle stages,namely,proestrus,estrus,metestrus and diestrus.Short-term treatment(1 week duration)exhibited high frequency of appearance of proestrus and estrus stages while mid-(3 weeks)and long-term(6 weeks)treatments revealed constancy in the frequency of all stages irrespective to animal groups.The plasma and organ lipid profile,as well as ovary,uterus and liver growth remained unchanged when compared to distilled water-treated animals.Following long-term administration of plant extract(6 weeks),no adverse effect was noticed.Conclusions:Our data partially support the use of F.asperifolia in common medicine. | Esther Ngadjui Pierre Watcho Telesphore Benoit Nguelefack Albert Kamanyi | 2013 | Asian Pacific Journal of Tropical Biomedicine2013，3，1： | 3 |
| 3 | Androgenic effect of Mondia whitei roots in male rats显示文摘Aim: To determine the effect of the aqueous extract of Mondia whitei (Periplocaceae) roots on testosterone production and fertility of male rats. Methods: Adult male Wistar rats were used. In the acute study, 20 rats were randomly divided into 5 groups of 4 animals each. Four treated groups were administered orally a single dose of Mondia whitei (400 mg/kg) and the controls received a similar amount of distilled water. One group of animals were sacrificed by cervical dislocation 1, 2, 4 and 6 h after treatment, respectively. The controls were sacrificed at 6 h.Testicular testosterone was determined by radioimmunoassay. In the chronic study, 28 rats were divided at random into 4 groups of 7 animals each: Groups 1, 2 and 3 were given orally the plant extract (400 mg·kg^-1·day^-1) for 2, 4 and 8 days, respectively. The animals of Groups 1 and 2 were sacrificed 24 hours after the last dosing. The controls (Group 4) received the same amount of distilled water for 8 days. The fertility was assessed only in Groups 3 and 4 and after that, the animals were sacrificed and the epididymal sperm density, the serum testosterone and the testicular testosterone and 17β-estradiol were assayed. The serum, testicular and epidydimal protein contents were also determined. Results: In the acute treatment groups, the serum and testicular concentrations of testosterone remained unchanged at all the time points. Chronic treatment for 8 days induced a significant increase in the testicular weight, the serum and testicular testosterone, the testicular protein content and the sperm density (P<0.05-0.01), but did not affect the accessory gland weights, the serum protein contents, the testicular concentration of 17β-estradiol and the fertility compared to the controls. Conclusion: Mondia whitei root extract possesses an androgenic property. (Asian J Androl 2004 Sep; 6: 269-272) | Pierre Watcho Pierre Kamtchouing Selestin D.Sokeng Paul F.Moundipa Justine Tantchou Jean L.Essame Noussithe Koueta | 2004 | Asian Journal of Andrology2004，6，3： | 2 |
| 4 | Prediabetic nephropathy as an early consequence of the high-calorie/high- fat diet: relation to oxidative stress 显示文摘 | Shevalye H Lupachyk S Watcho P | 2012 | Endocrinology2012，153，3： | 1 |
| 5 | Endoplasmic reticulum stress plays a key role in the pathogenesis of diabetic peripheral neuropathy显示文摘 | Lupachyk S Watcho P Stavniichuk R | 2013 | Diabetes2013，62，3： | 1 |
| 6 | Endoplasmic reticulum stress plays a key role in the pathogenesis of diabetic peripheral neu- ropathy显示文摘 | Lupachyk S Watcho P Stavniichuk R | 2013 | Diabetes2013，62，3： | 1 |
| 7 | Effects of Dracaena arborea(Dracaenaceae) on sexual dysfunction in 4 weeks hyperglycemic male rats显示文摘Objective:To investigate the effects of Dracaena arborea(D.arborea) on the sexual behavior parameters in experienced type-1 diabetic rats.Methods:Aqueous and ethanol(100 and 500mg/kg respectively)extracts of dried root barks of D.arborea.sildenafil citrate(1.44 mg/kg),trimethylamine-N-oxide(TMAO,20 mg/kg)and distilled water(110 mL/kg.were orally administered to 4 weeks streptozolocin-induced diabetic rats.Mount latency and frequency(ML.MF),intromission latency and frequency(IL,IF) and post-ejaculatory interval(PEI) were measured by ejaculating series during 90 min once a week for 4 weeks.Glycemia was determined at the beginning and at the end of the treatment Results:D.arborea did not show any major antihvperglycemic effects.Compared to the control group,a significant(P<0.05-0.001)increase in MF and IF was noticed in rats treated with sildenafil citrate(89.71% and 90.07% respectively),aqueous(500 mg/kg.88.08%and 88.749;respectively) and ethanol(100 mg/kg;89.53%and 89.17respectively) extracts of D.arborea after two weeks(series 1) of treatment.ML,IL and PEI were significantly(P<0.05-0.001) decreased after 4 weeks of daily treatment[sildenafil citrate(96.31.96.31%and 34.98%),and D.arborea aqueous 500 mg/kg(94.33.94.33% and 66.609;i and ethanol extracts 100 mg/kg(96.98.97.089;and 64.26%)].Conclusions:These aphrodisiac potentials of D.arborea in experienced diabetic rats could be due to the antioxidant and androgenic properties of phenols,flavonoids.saponins and sterols revealed in the plant extracts. | Wankeu-Nya M Watcho P Nguelefack TB Cairo-Juarez M Tapondjou L Kamanyi A | 2014 | Asian Pacific Journal of Tropical Medicine2014，7，8： | 1 |
| 8 | Endoplasmic reticulum stress contributes to prediabetic peripheral neuropathy显示文摘 | Sergey Lupachyk Pierre Watcho Alexander A. Obrosov Roman Stavniichuk Irina G. Obrosova | 2012 | Experimental Neurology2012，，： | 1 |
| 9 | Evaluation of In Vitro Uterotonic Activities of Fruit Extracts of Ficus asperifolia in Rats显示文摘 | Pierre Watcho Esther Ngadjui Pepin Alango Nkeng-Efouet Telesphore Beno?t Nguelefack Albert Kamanyi | 2011 | Evidence-Based Complementary and Alternative Medicine2011，，： | 1 |
| 10 | Endoplasmic reticulum stress plays a key role in the pathogenesis of diabetic peripheral neuropathy显示文摘 | Lupachyk S Watcho P Stavniichuk R | 2013 | Diabetes2013，62，3： | 1 |
| 11 | Prediabetic nephropathy as an early consequence of the high - calorie/high - fat diet : relation to oxi- dative stress显示文摘 | Shevalye H Lupachyk S Watcho P | 2012 | Endocrinology2012，153，3： | 1 |
| 12 | Endoplasmic reticulum stress plays a key role in the pathogenesis of diabetic peripheral neu- ropathy显示文摘 | Lupachyk S Watcho P Stavniichuk R | 2013 | Diabetes2013，62，3： | 1 |
| 13 | Poly(ADP-ribose) polymerase-1 (PARP-1) gene deficiency alleviates diabetic kidney disease显示文摘 | Shevalye H Maksimchyk Y Watcho P | 2010 | Biochim Biophys Acta2010，1802，11： | 1 |
| 14 | Effects of pirirniphos-methyl (an organophosphate insecticide) on the fertility of adult male rats 显示文摘 | Ngoula F Watcho P Dongmo M C et a! | 2007 | Mr Health Sci2007，7，1： | 1 |
| 15 | Endoplasmic reticulum stress plays a key role in the pathogenesis of dia- betic peripheral neuropathy 显示文摘 | Lupachyk S Watcho P Stavniichuk R | 2013 | Diabetes2013，62，3： | 1 |
| 16 | Endoplasmic retieulum stress plays a key role in the pathogenesis of diabetic peripheral neuropathy 显示文摘 | Lupachyk S Watcho P Stavniichuk R | 2013 | Diabetes2013，62，3： | 1 |
| 17 | Triglyce- ride, nonesterified fatty acids, and prediabetic neuropathy : role for oxidative - nitrosative stress显示文摘 | LUPACHYK S WATCHO P HASANOVA N | 2012 | Free Radic Bio Med2012，52，8： | 1 |
| 18 | Endoplasmic reticulum stress plays a key role in the pathogenesis of diabetic peripheral neuropathy 显示文摘 | Lupachyk S Watcho P Stavniichuk R | 2013 | Diabetes2013，62，3： | 1 |
| 19 | Effects of pirimiphos-methyl (an organophosphate insecticide )on the fertility of adult male rats 显示文摘 | Ferdinand Ngoula Pierre Watcho Marie-Chantal Dongmo | 2007 | African Health Sciences2007，7，1： | 1 |
| 20 | Poly(ADPribose)polymerase-1(PARP-1)gene deficiency alleviates diabetic kidney disease显示文摘 | Shevalye H Maksimchyk Y Watcho P | 2010 | Biochim Biophys Acta2010，，： | 1 |
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