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| 1 | Intravenous vs intraperitoneal mesenchymal stem cells administration:What is the best route for treating experimental colitis?显示文摘AIM:To investigate the therapeutic effects of mesenchymal stem cells(MSCs)transplanted intraperitoneally andintravenously in a murine model of colitis.METHODS:MSCs were isolated from C57BL/6 mouse adipose tissue.MSC cultures were analyzed according to morphology,cellular differentiation potential,and surface molecular markers.Experimental acute colitis was induced in C57BL/6 mice by oral administration of2%dextran sulfate sodium(DSS)in drinking water ad libitum from days 0 to 7.Colitis mice were treated with1×106 MSCs via intraperitoneal or intravenous injection on days 2 and 5.The disease activity index was determined daily based on the following parameters:weight loss,stool consistency and presence of blood in the feces and anus.To compare morphological and functional differences in tissue regeneration between different MSC injection modalities,mice were euthanized on day 8,and their colons were examined for length,weight,and histopathological changes.Inflammatory responses were determined by measuring the levels of different serum cytokines using a CBA Th1/Th2/Th17 kit.Apoptotic rates were evaluated by terminal deoxynucleotidyl transferase-mediated d UDPbiotin nick end labeling assay.RESULTS:Intravenous infusion of MSCs was more effective than intraperitoneal treatment(P<0.001)in reducing the clinical and histopathologic severity of colitis,which includes weight loss,diarrhea and inflammation.An histological evaluation demonstrated decreased colonic inflammation based on reduced crypt loss and reduced infiltration of inflammatory cells.This therapeutic effect was most likely mediated by the down-regulation of pro-inflammatory cytokines[interleukin(IL)-6 and tumor necrosis factor(TNF)];and by the up-regulation of anti-inflammatory cytokines(IL-10 and IL-4).Intravenous transplantation alsoinduced high levels of IFN that lead to activation of the immunosuppressive activity of the MSCs,which did not occur with intraperitoneal transplantation(P=0.006).An increase in apoptotic T cells was observed after intravenous,but not intraperitoneal,MSC infusion,suggesting that MSCs can induce apoptosis in resistant T cells in colonic inflammation(P=0.027).CONCLUSION:Our results demonstrate that intravenous treatment is a superior method for reducing colon inflammation compared with intraperitoneal therapy. | Fabiany da Costa Goncalves Natália Schneider Fernanda Otesbelgue Pinto Fabíola Schons Meyer Fernanda Visioli Bianca Pfaffenseller Patrícia Luciana da Costa Lopez Eduardo Pandolfi Passos Elizabeth Obino Cirne-Lima Luíse Meurer Ana Helena Paz | 2014 | World Journal of Gastroenterology2014，20，48： | 4 |
| 2 | Set-point weight tuning rules for fractional-order PID controllers 显示文摘 | PADULA F VISIOLI A | 2013 | Asian Journal of Control2013，15，3： | 1 |
| 3 | A two degree of freedom PI controller based on events显示文摘 | Sancheza J Visioli A Dormidoa S | 2011 | Journal of Process Control2011，21，4： | 1 |
| 4 | Global Minimum-Time Trajectory Planning of Mechanical Manipulators Using Interval Analysis显示文摘 | Piazzi A Visioli A | 1998 | International Journal of Control1998，71，4： | 1 |
| 5 | Modified anti-windup scheme for PID controller 显示文摘 | VISIOLI A | 2003 | IEE Proceedings Control Theory and Applications2003，150，1： | 1 |
| 6 | Recurrence aftertreatment by loop electrosurgical excision procedure (LEEP)of high-grade cervical intraepithelial neoplasia 显示文摘 | Cecchini S Visioli CB Zappa M | 2002 | Tumor2002，88，6： | 1 |
| 7 | A noncausal approach for PID control 显示文摘 | PIAZZI A VISIOLI A | 2006 | Journal of Process Control2006，16，： | 1 |
| 8 | Antioxidant and other biological activities of phenols from olive and olive oil显示文摘 | VISIOLI F POLI A | 2002 | Med Res Rev2002，22，1： | 1 |
| 9 | Direct analysis of total antioxidant activity of olive oil and studies on the influence of heating 显示文摘 | Pellegrini N Visioli F Buratti S | 2001 | Journal of Agricultural and Food Chemistry2001，49，： | 1 |
| 10 | Metal tolerance and hyperaccumula- tion:Costs and trade-offs between traits and environment显示文摘 | Maestri E Marmiroli M Visioli G | 2010 | Environ- mental and Experimental Botany2010，68，1： | 1 |
| 11 | Performance assess- ment and retuning of PID controllers for integral processes 显示文摘 | Massimiliano Veronesi Antonio Visioli | 2010 | JournalofProcess Control2010，20，： | 1 |
| 12 | Direct analysis of total antioxidant activity of olive oil and studies on the influence of heating显示文摘 | Pellegrini N Visioli F Buratti S | 2001 | Journal of Agricultural and Food Chemistry2001，49，： | 1 |
| 13 | On the Stabilizing PID Controllers for Integral Processes显示文摘 | Padula F Visioli A | 2012 | IEEE Trans on Automatic Control2012，57，： | 1 |
| 14 | Global minimum-time trajectory planning of mechanical manipulators using interval analysis显示文摘 | Pizza A Visioli A | 1998 | Interna- tional Journal of Control1998，71，4： | 1 |
| 15 | Sensitivity, specificity, and predictive accuracies of non-invasive tests, singly and in combination, for diagnosis of hibernating myocardium 显示文摘 | Rahimtoola SH Visioli O | 2000 | Eur Heart J2000，21，16： | 1 |
| 16 | A new design for a PID plus feedforward controller显示文摘 | VISIOLI A | 2004 | Journal of Process Control2004，14，4： | 1 |
| 17 | Two-dimen- sional liquid chromatography technique coupled with mass spectrometry analysis to compare the proteomic response to cadmium stress in plants 显示文摘 | VISIOLI G MARMIROLI M MARMIROLI N | 2010 | Journal of Biomedicine and Biotechnology2010，，56： | 1 |
| 18 | Global minimum- jerk trajectory planning of ro- bot manipulators 显示文摘 | Piazzi A Visioli A | 2000 | IEEE Transactions on Industrial Electronics2000，47，1： | 1 |
| 19 | Direct analysis of total antioxidant activity of olive oil and studies on the influence of heating 显示文摘 | Pellegrini N Visioli F Buratti S | 2001 | Agric Food Chem2001，49，： | 1 |
| 20 | Global minimum-jerk trajectory planning of robot manipulator显示文摘 | Piazzi A Visioli A | 2000 | IEEE Transactions on Industrial Elec- tronics2000，47，1： | 1 |
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