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| 1 | Human Mesenchymal Stem Cells as a Gene Delivery System to Create Cardiac Pacemakers显示文摘 | Irina Potapova Alexei Plotnikov Zhongju Lu Peter Danilo Virginijus Valiunas Jihong Qu Sergey Doronin Joan Zuckerman Iryna N. Shlapakova Junyuan Gao Zongming Pan Alan J. Herron Richard B. Robinson Peter R. Brink Michael R. Rosen Ira S. Cohen | 2004 | Circulation Research: Journal of the American Heart Association2004，，7： | 1 |
| 2 | Fluorescence Lifetime Heterogeneity in Aggregates of LHC Ⅱ Revealed by Time-resolved Microscopy 显示文摘 | VIRGINIJUS B CEES J G JURRIEN V | 2001 | Biophysical Journal2001，81，1： | 1 |
| 3 | Selective permeability of gap junction channels显示文摘 | Gary S. Goldberg Virginijus Valiunas Peter R. Brink | 2004 | BBA - Biomembranes2004，，： | 1 |
| 4 | Humanconnexin 26 and connexin 30 form functional heteromeric andheterotypic channels显示文摘 | SABRINA WY JUNXIAN Z VIRGINIJUS V | 2007 | Am J Physiol Cell Physiol2007，293，3： | 1 |
| 5 | Size dependency of circular dichroism in macroaggregates of photosynthetic pigment-protein complexes显示文摘 | Virginijus B Laszl6 M Gyozo G | 1994 | Biochemistry1994，33，10： | 1 |
| 6 | Structure and principles of self-assembly of giant“sea urchin”type sulfonatophenyl porphine aggregates显示文摘Principles of molecular self-assembly into giant hierarchical structures of hundreds of micrometers in size are studied in aggregates of meso-tetra(4-sulfonatophenyl)porphine(TPPS_(4)).The aggregates form a central tubular core,which is covered with radially protruding filamentous non-branching aggregates.The filaments cluster and orient at varying angles from the core surface and some filaments form bundles.Due to shape resemblance,the structures are termed giant sea urchin(GSU)aggregates.Spectrally resolved fluorescence microscopy reveals J-and H-bands of TPPS_(4)aggregates in both the central core and the filaments.The fluorescence of the core is quenched while filaments exhibit strong fluorescence.Upon drying,the filament fluorescence gets quenched while the core is less affected,showing stronger relative fluorescence.Fluorescencedetected linear dichroism(FDLD)microscopy reveals that absorption dipoles corresponding to J-bands are oriented along the filament axis.The comparison of FDLD with scanning electron microscopy(SEM)reveals the structure of central core comprised of multilayer ribbons,which wind around the core axis forming a tube.Polarimetric second-harmonic generation(SHG)and thirdharmonic generation microscopy exhibits strong signal from the filaments with nonlinear dipoles oriented close to the filament axis,while central core displays very low SHG due to close to centrosymmetric organization.Large chiral nonlinear susceptibility points to helical arrangement of the filaments.The investigation shows that TPPS_(4)molecules form distinct aggregate types,including chiral nanotubes and nanogranular aggregates that associate into the hierarchical GSU structure,prototypical to complex biological structures.The chiral TPPS_(4)aggregates can serve as harmonophores for nonlinear microscopy. | Marijus Pleckaitis Fayez Habach Lukas Kontenis Gábor Steinbach Greta Jarockyte Agne Kalnaityte IldikóDomonkos Parveen Akhtar Mehdi Alizadeh Saulius Bagdonas Vitalijus Karabanovas GyőzőGarab Ricardas Rotomskis Virginijus Barzda | 2022 | Nano Research2022，15，6： | 0 |
| 7 | Comparison of genomic and transcriptional microbiome analysis in gastric cancer patients and healthy individuals显示文摘BACKGROUND Helicobacter pylori and the stomach microbiome play a crucial role in gastric carcinogenesis,and detailed characterization of the microbiome is necessary for a better understanding of the pathophysiology of the disease.There are two common modalities for microbiome analysis:DNA(16S rRNA gene)and RNA(16S rRNA transcript)sequencing.The implications from the use of one or another sequencing approach on the characterization and comparability of the mucosal microbiome in gastric cancer(GC)are poorly studied.AIM To characterize the microbiota of GC using 16S rRNA gene and its transcript and determine difference in the bacterial composition.METHODS In this study,316 DNA and RNA samples extracted from 105 individual stomach biopsies were included.The study cohort consisted of 29 healthy control individuals and 76 patients with GC.Gastric tissue biopsy samples were collected from damaged mucosa and healthy mucosa at least 5 cm from the tumor tissue.From the controls,healthy stomach mucosa biopsies were collected.From all biopsies RNA and DNA were extracted.RNA was reverse transcribed into cDNA.V1-V2 region of bacterial 16S rRNA gene from all samples were amplified and sequenced on an Illumina MiSeq platform.Bray-Curtis algorithm was used to construct sample-similarity matrices abundances of taxonomic ranks in each sample type.For significant differences between groups permutational multivariate analysis of variance and Mann-Whitney test followed by false-discovery rate test were used.RESULTS Microbial analysis revealed that only a portion of phylotypes(18%-30%)overlapped between microbial profiles obtained from DNA and RNA samples.Detailed analysis revealed differences between GC and controls depending on the chosen modality,identifying 17 genera at the DNA level and 27 genera at the RNA level.Ten of those bacteria were found to be different from the control group at both levels.The key taxa showed congruent results in various tests used;however,differences in 7 bacteria taxa were found uniquely only at the DNA level,and 17 uniquely only at the RNA level.Furthermore,RNA sequencing was more sensitive for detecting differences in bacterial richness,as well as differences in the relative abundance of Reyranella and Sediminibacterium according to the type of GC.In each study group(control,tumor,and tumor adjacent)were found differences between DNA and RNA bacterial profiles.CONCLUSION Comprehensive microbial study provides evidence for the effect of choice of sequencing modality on the microbiota profile,as well as on the identified differences between case and control. | Darja Nikitina Konrad Lehr Ramiro Vilchez-Vargas Laimas Virginijus Jonaitis Mindaugas Urba Juozas Kupcinskas Jurgita Skieceviciene Alexander Link | 2023 | World Journal of Gastroenterology2023，29，7： | 0 |
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