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| 1 | Obstructive sleep apnea syndrome and cardiovascular disease: The influence of C-reactive protein显示文摘Obstructive sleep apnea syndrome(OSAS) is a common medical condition, associated with atherosclerosis and cardiovascular disease(CVD). The underlying pathophysiologic mechanisms of this association have not been completely understood and may be multifactorial in origin. A number of studies suggest that inflammatory processes have emerged critical in the pathogenesis of CVD in OSAS. A range of circulating inflammatory molecules has been identified and measured, with a view to assess inflammation and predict vascular damage risk, such as plasma cytokines, adhesion molecules, and C-reactive protein(CRP). CRP is a relevant marker worthy of further study, because not only is elevated in patients with OSAS, but also is rapidly becoming a risk factor for cardiac disease. Furthermore, in selected OSAS patients, aggressive treatment of the disorder may lead to retarding or even improvement of CVD progression. However, still there is a debate on the true correlation between CRP and OSAS, as well as the clinical effect of any reduction after OSAS treatment. Further research is required to define those OSAS patients who will have a considerable reduction with treatment, as well as to understand the significance of the interaction between cardiovascular risk factor and CRP reduction in patients with OSAS. | Izolde Bouloukaki Charalampos Mermigkis Eleftherios M Kallergis Violeta Moniaki Eleni Mauroudi Sophia E Schiza | 2015 | World Journal of Experimental Medicine2015，5，2： | 12 |
| 2 | High levels of homocysteine downregulate apolipoprotein E expression via nuclear factor kappa B显示文摘AIM: To investigate the effect of high homocysteine(Hcy) levels on apolipoprotein E(apoE) expression and the signaling pathways involved in this gene regulation.METHODS: Reverse transcriptase polymerase chain reaction(RT-PCR) and Western blot were used to assess apo E expression in cells treated with various concentrations(50-500 μmol/L) of Hcy. Calcium phosphatetransient transfections were performed in HEK-293 and RAW 264.7 cells to evaluate the effect of Hcy on apoE regulatory elements [promoter and distal multienhancer 2(ME2)]. To this aim, plasmids containing the proximal apoE promoter [(-500/+73)apoE construct] alone or in the presence of ME2 [ME2/(-500/+73)apoE construct] to drive the expression of the reporter luciferase gene were used. Co-transfection experiments were carried out to investigate the downstream effectors of Hcymediated regulation of apoE promoter by using specific inhibitors or a dominant negative form of IKβ. In other co-transfections, the luciferase reporter was under the control of synthetic promoters containing multiple specific binding sites for nuclear factor kappa B(NF-κB), activator protein-1(AP-1) or nuclear factor of activated T cells(NFAT). Chromatin immunoprecipitation(ChI P)assay was accomplished to detect the binding of NF-κB p65 subunit to the apoE promoter in HEK-293 treated with 500 μmol/L Hcy. As control, cells were incubated with similar concentration of cysteine. NF-κB p65 proteins bound to DNA were immunoprecipitated with anti-p65 antibodies and DNA was identified by PCR using primers amplifying the region-100/+4 of the apoE gene. RESULTS: RT-PCR revealed that high levels of Hcy(250-750 μmol/L) induced a 2-3 fold decrease in apoE m RNA levels in HEK-293 cells, while apo E gene expression was not significantly affected by treatment with lower concentrations of Hcy(100 μmol/L). Immunoblotting data provided additional evidence for the negative role of Hcy in apoE expression. Hcy decreased apoE promoter activity, in the presence or absence of ME2, in a dose dependent manner, in both RAW 264.7 and HEK-293 cells, as revealed by transient transfection experiments. The downstream effectors of the signaling pathways of Hcy were also investigated. The inhibitory effect of Hcy on the apo E promoter activity was counteracted by MAPK/ERK kinase 1/2(MEK1/2) inhibitor U0126, suggesting that MEK1/2 is involved in the downregulation of apoE promoter activity by Hcy. Our data demonstrated that Hcy-induced inhibition of apoE took place through activation of NF-κB. Moreover, we demonstrated that Hcy activated a synthetic promoter containing three NF-κB binding sites, but did not affect promoters containing AP-1 or NFAT binding sites. ChI P experiments revealed that NF-κB p65 subunit is recruited to the apoE promoter following Hcy treatment of cells.CONCLUSION: Hcy-induced stress negatively modulates apoE expression via MEK1/2 and NF-κB activation. The decreased apo E expression in peripheral tissues may aggravate atherosclerosis, neurodegenerative diseases and renal dysfunctions. | Violeta G Trusca Adina D Mihai Elena V Fuior Ioana M Fenyo Anca V Gafencu | 2016 | World Journal of Biological Chemistry2016，7，1： | 6 |
| 3 | Purification and characterization of cell wall-bound peroxidase from vanilla bean显示文摘 | Ofelia Márquez Krzysztof N. Waliszewski Rosa M. Oliart Violeta T. Pardio | 2007 | LWT - Food Science and Technology2007，，8： | 2 |
| 4 | Dynamic electronic speckle pattern interferometry( DESPI)phase analyses with temporal Hilbert transfrom 显示文摘 | Violeta D M Hirofumi K | 2003 | Optics Express2003，11，6： | 1 |
| 5 | Prevalence and epidemiological features of bovine viral diarrhoea virus infection in Lithuania显示文摘 | Violeta M Algirdas S Raimundas M | 2004 | Veterinary Microbiology2004，99，： | 1 |
| 6 | General Practitioners’ Knowledge, Attitudes, Beliefs, and Practices in the Management of Sexual Dysfunction—Results of the Portuguese SEXOS Study显示文摘 | Violeta Alarc?o Sofia Ribeiro Filipe Le?o Miranda Mário Carreira Teresa Dias Joaquim Garcia e Costa Alberto Galv?o‐Teles | 2012 | The Journal of Sexual Medicine2012，，： | 1 |
| 7 | Dynamic electronic speckle pattern interferometry (DESPI) phase analyses with temporal Hilbert transform显示文摘 | Violeta D M Hirofumi K | 2003 | Optics Express(S1094-4087)2003，11，6： | 1 |
| 8 | Genetically engineered grapevines carrying GFLV coat proteinand antifreeze genes显示文摘 | Georgy P G Ivan J T Violeta M C | 2001 | Agriculturae Conspectus Scientificus2001，66，1： | 1 |
| 9 | Application of an indirect ELISA and a PCR technique for detection of cows'milk in sheep's and goats'milk cheeses显示文摘 | Ine's M Lo'pez-Catleja Isabel Gonza'lez Violeta Fajardo | 2007 | International Dariy Journal2007，17，1： | 1 |
| 10 | Apoptosis and active caspase-3 expression in human granulose cells显示文摘 | Katarina V Saraga M | 2005 | Fertility and Sterility2005，83，2： | 1 |
| 11 | Apoptosis and active caspase-3 expression in human granulosa cells显示文摘 | Violeta G Katarina V Saraga M | 2005 | Fertility and Sterility2005，83，2： | 1 |
| 12 | Synthesis of oxido-bridged analogs of 18-hydroxyprogesterone显示文摘 | Violeta Benedetti Doctorovich M O Ghini A A Burton G | 1996 | Steroids1996，61，： | 1 |
| 13 | Prevalence of sleep apnea and electrocardiographic disturbances in morbidly obese patients显示文摘 | VALENCIA-FLORES M OREA A VIOLETA A | 2000 | Obes Res2000，8，： | 1 |
| 14 | Dynamic electronic speckle pattern interferometry (DESPI) phase analyses with temporal Hilbert transform显示文摘 | Violeta D M Hirofumi K | 2003 | Optics Express2003，11，6： | 1 |
| 15 | Temporal and Spatial Virations of Metal Content in TSP and PM-0 in Mexico City during 1996--1998 显示文摘 | Violeta M Marisela M Miguel T | 2002 | Journal of Aerosol Science2002，33，1： | 1 |
| 16 | Metal complexes with Schiff-base hgandspyridoxal and semicarbazide-based derivatives 显示文摘 | VUKADIN M Leovac VIOLETA S Jevtovic LJILANA S Jovanovic | 2005 | J Serb Chem Soc2005，70，3： | 1 |
| 17 | Prevalence and epidemiological features of bovine viral diarrhoea virus infec-tion in Lithuania 显示文摘 | Violeta M Algirdas E Raimundas M | 2004 | Vet Microbiololy2004，99，： | 1 |
| 18 | Prevalence and epidemiological features of bovine viral diarrhoea virus infection in Lithuania显示文摘 | Violeta M Algirdas S Raimundas M Saulius P | 2004 | Veterinary Microbiology2004，99，： | 1 |
| 19 | Dynamic electronic speckle pattern intefferometry (DESPI) phase analyses with temporal Hilbert transform显示文摘 | Violeta D M Hirofumi K | 2003 | Optics Express2003，11，6： | 1 |
| 20 | Possibilities for improving the cold flow properties of biodiesel fuel by blending with butanol显示文摘 | VIOLETA M KIRIL K IRINA K | 2015 | Renewable Energy2015，75，3： | 1 |
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