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| 1 | Antibacterial activity and characterization of secondary metabolites isolated from mangrove plant Avicennia officinalis显示文摘Objective:To explore antibacterial activity and characterization of secondary metabolites isolated from mangrove plant Avicennia officinalis(A.officinalis).Methods:In the present study the leaf extracts of A.officinalis were examined for its antibacterial potential using five different solvents against some reference strains of human pathogenic bacteria for the crude extract.Maximum activity was observed for ethyl acetate and hence different concentrations like 15μL,25μL,and 50μL of ethyl extracts was checked for its antibacterial activity.Partial purification of crude extract was carried by column chromatography and fractions were analyzed using gas chromatography-mass spectrometry(GC-MS) to identify compounds.Results:The crude ethyl acetate extracts of A.officinalis showed remarkable antibacterial activity with zones of inhibition of 13 mm against Eschericia coli(E.coli) and 11 mm against Staphylococcus aureus (S.aureus).Fraction 13(ethyl acetate:methanol= 8:2) as the most potent one against with the minimal inhibitory concentration of 30 mm against E.coli and 25 mm against S.aureus.The GCMS resultsof active column fraction(F13) revealed that the active principals were a mixture of hydroxy- 4 methoxybenzoic acid,diethyl phthalate,oleic acid.Conclusions:The leaf extracts with proven antibacterial effects can clearly be directed towards cancer treatment as to inhibiting cancer cell growth.The limited number of test organisms owes to a constraint of resource.So,the effect of strong bursts of leaf extracts on human pathogenic bacteria should further be tested on a wide range of test organisms. | Valentin Bhimba B J Meenupriya Elsa Lycias Joel D Edaya Naveena Suman kumar M Thangaraj | 2010 | Asian Pacific Journal of Tropical Medicine2010，3，7： | 13 |
| 2 | Characterization of cytotoxic compound from mangrove derived fungi Irpex hydnoides VB4显示文摘Objective:To investigate the cytotoxic activity of endophytic fungi isolated from mangrove fungi.Methods:In the present study the DNA was isolated and the ITS region of 5.8s rRNA was amplified using specific primers ITS 1 and ITS4 and sequence was determined using automated sequencers.Blast search sequence similarity was found against the existing non redundant nucleotide sequence database thus,identified as Aspergilus flavus,Hyporcaea lixii,Aspergillus niger,Eutorium amstelodami,Irpex hydnoides and Neurospora crassa.Among the seven isolates, one fungi Irpex hydnoides was selected for further studies.The fungi were grown in sabouraud broth for five days and filtrate were separated and subjected to ethyl acetate for further studies. Results:Nearly half(49.25%) of the extracts showed activity(IC_(50) of 125 μ g/mL).These values were within the cutoff point of the National Cancer Institute criteria for cytotoxicity(IC_(50)<20 μ g/mL) in the screening of crude plant extracts.The GC MS analysis revealed that the active principals might be Tetradecane(6.26%) with the RT 8.606.Conclusions:It is clear from the present study that mangrove fungi with bioactive metabolites can be expected to provide high quality biological material for high throughout biochemical,anti cancer screening programmes.The results help us conclude that me potential of using metabolic engineering and post genomic approaches to isolate more novel bioactive compounds and to make their possible commercial application is not far off. | B Valentin Bhimba DA Agnel Defora Franco Geena Mary Jose Jibi Merin Mathew Elsa Lycias Joel | 2011 | Asian Pacific Journal of Tropical Biomedicine2011，1，3： | 3 |
| 3 | Antibacterial screening of silver nanoparticles synthesized by marine micro algae显示文摘Objective:To explore the biosynthesis of silver nanoparticles synthesized by marine microalgae. Methods:Marine microalgae was collected from Central Marine Fisheries Research Institute (CMFRI,tuticorin) and cultured in the lab.Silver nanoparticles synthesis were observed in normal and microwave irradiated microalgae and screened against human pathogens for the presence of antimicrobials.Results:The presence of silver nanoparticle was confirmed by UV-Visible spectroscopy at 420 nm by the presence of plasmon peak.Further confirmation was done by scanning electron microscope(SEM).Conclusions:These results not only provide a base for further research but are useful for drug development in the present and future. | D Devina Merin S Prakash B Valentine Bhimba | 2010 | Asian Pacific Journal of Tropical Medicine2010，3，10： | 2 |
| 4 | Expression ofa reflex of snapping/biting response to amino acids prior to the first exogenous feeding in salmonid alevins显示文摘 | Valentin(c)i(c) T B Lamb C F Caprio J | 1999 | Physiol Behav1999，67，4： | 1 |
| 5 | Diagnosis of masses presenting within the venticles on computed tomography显示文摘 | Kendall B Reider G Valentine A | 1983 | Neuroradiology1983，25，1： | 1 |
| 6 | Fusion of a kinase gene, ALK, to a nucleolar protein gene, NPM, in non-Hodgkin′s lymphoma显示文摘 | Morris S W Kirstein M N Valentine M B | 1994 | Science1994，263，5151： | 1 |
| 7 | Diagnosis of masses presenting within the ventricules on computed tomography显示文摘 | Kendall B Reider G Valentine A | 1983 | Neuroradiology1983，25，： | 1 |
| 8 | Patient sety in intensive care: results from the multinational Sentinel Events Evaluation (SEE) study显示文摘 | Valentin A Capuzzo M Guidet B | 2006 | Intensive care medicine2006，32，10： | 1 |
| 9 | AMPK activation: a therapeutic target for type 2 diabetes?显示文摘 | Coughlan K A Valentine R J Ruderman N B | 2014 | Diabetes Metab Syndr Obes2014，24，7： | 1 |
| 10 | Abdominal aortic aneurysms and malignant neoplasia:double jeopardy显示文摘 | Valentine B J Pearson AS McInfire DD | 1998 | Surgery1998，123，2： | 1 |
| 11 | Meta-analysis of risk factors for posttraumatic stress disorder in trauma-exposed adults显示文摘 | Andrews B Valentine JD | 2000 | J Consult Clin Psychol2000，68，5： | 1 |
| 12 | Soil seed bank composition and diversity in a managed temperate deciduous forest显示文摘 | DECOCQ G VALENTIN B TOUSSAINT B | 2004 | Biodiversity and Conservation2004，13，： | 1 |
| 13 | High levels of dietary vitamin E do not replace celluar glutathione peroxidase in protecting mice from acute oxidative stress 显示文摘 | CHENG W H VALENTINE B A LEI X G | 1999 | J Nutr1999，129，： | 1 |
| 14 | Crossover/non-crossover dif- ferentiation, synaptonemal complex formation, and regulatory surveil- lance at the leptotene/zygotene transition of meiosis显示文摘 | Valentin B G Kleckner N Hunter N | 2004 | Cell2004，117，1： | 1 |
| 15 | 查看详情显示文摘 | A B Sorokin F Quignard R Valentin | | 0，，： | 1 |
| 16 | Meta-analysis of risk factors for posttraumatic stress disorder in trauma-exposed adults显示文摘 | Brewin CR Andrews B Valentine JD | 2000 | J Consult Clin Psychol2000，68，5： | 1 |
| 17 | Effects of temperature on scope for growth and accumulation of Cd, Co, Cu and Pb by the marine bivalve Mytilus edulis 显示文摘 | Valentine K M Ronny B | 2007 | Marine Environmental Research2007，63，3： | 1 |
| 18 | Effects of temperature on scope for growth and accumulation of Cd,Co,Cu and Pb by the marine bivalve Mytilns edulis显示文摘 | Valentine K M Bonny B | 2007 | Marine Environmental Research2007，63，： | 1 |
| 19 | Surface Structure of Stainless and Hadfield Steel after Impact Wear显示文摘 | Yuri N P Valentin G G Hans B | 2006 | Wear2006，260，6： | 1 |
| 20 | Caspase 8 is deleted or silenced preferentially in childhood neuroblastomas with amplification of MYCN显示文摘 | TEITZ T WEI T VALENTINE M B | 2000 | Nat Med2000，6，5： | 1 |
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