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| 1 | Characterization of spermidine hydroxycinnamoyl transferases from eggplant (Solanum melongena L.) and its wild relative Solanum richardii Dunal显示文摘Eggplant produces a variety of hydroxycinnamic acid amides(HCAAs)that have an important role in plant development and adaptation to environmental changes.In this study,we identified and characterized a spermidine hydroxycinnamoyl transferase(SHT)from eggplant(Solanum melongena)and its wild relative S.richardii,designated as SmSHT and SrSHT,respectively.SmSHT was abundant in flowers and fruits,whereas the level of SrSHT was remarkably low in all tissues.Heat-shock/drought treatment stimulated the expression of SmSHT in both leaves and fruits,indicating its involvement in plant stress response.Both SHT polypeptides had extremely high identity with just five amino-acid substitutions.Recombinant SmSHT catalyzed the synthesis of mono-,bi-and tri-acylated polyamines.Using caffeoyl-CoA as the acyl donor,SmSHT preferred spermidine as the acyl acceptor.When spermidine was the acyl acceptor,the donor preference order for SmSHT was caffeoyl-CoA>feruloyl-CoA>ρ-coumaroyl-CoA.SrSHT exhibited the same substrate specificity as SmSHT,yet exhibited significantly higher catalytic activity than SmSHT.For example,under caffeoyl-CoA and spermidine,Kcat of SrSHT was 37.3%higher than SmSHT.Molecular modeling suggests that five amino-acid substitutions in SrSHT result in four alterations in their predicted 3D structures.In particular,the conserved Lys402 adjacent to the DFGWG motif,and Cys200 in the crossover loop in SmSHT were replaced by Glu and Ser in SrSHT.These substitutions may contribute to the enhanced activity in SrSHT.Our study provides a platform to generate HCAA rich fruits for eggplant and other solanaceous crops. | Hui Peng Tianbao Yang Bruce D Whitaker Frances Trouth Lingfei Shangguan Wen Dong Wayne M Jurick | 2016 | Horticulture Research2016，3，1： | 2 |
| 2 | Myasthenia gravis:a review显示文摘 | Jayam Trouth A Dabi A Solieman N | | 0，，： | 1 |
| 3 | State Governance Patterns for Community Colleges显示文摘 | Lovell C D Trouth C | 2002 | New Directions for Community Colleges2002，2002，117： | 1 |
| 4 | Benign subarachnoid space enlargement of infancy显示文摘 | Suara RO Trouth AJ Collins M | 2001 | J Natl Med Assoc2001，93，2： | 1 |
| 5 | Myasthenia Gravis: A Review显示文摘 | Annapurni Jayam Trouth Alok Dabi Noha Solieman Mohankumar Kurukumbi Janaki Kalyanam Corrado Betterle | 2012 | <journal-title>Autoimmune Diseases2012，，： | 1 |
| 6 | Chemosensitivity of respiratory pacemaker neurons in the pre-Botzinger complex in vitro 显示文摘 | Johnson SM Trouth CO Smith JC | 1998 | Neu- rusei1998，32，： | 1 |
| 7 | Myasthenia gravis : a review显示文摘 | Jayam Trouth A Dabi A Solieman N | 2012 | Autoimmune Dis2012，2012，： | 1 |
| 8 | 文化负担:美国社会中前苏联移民的健康状况研究显示文摘一、研究背景在美国,大概有100万前苏联移民。尽管在政策争议以及文艺发展方面,前苏联移民的光辉已经被拉丁美洲人和亚洲人所取代,但他们依然有着诸多值得关注的特质。在发达国家里,前苏联移民已经成为最糟的健康指示器,他们的不良健康状况与文化习俗相关。 | Erin Trouth Hofmann 何肇红(译) 巫艳纯(译) | 2011 | 广西人文医学发展报告2011，，1： | 0 |
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