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| 1 | Role of autophagy in differential sensitivity of hepatocarcinoma cells to sorafenib显示文摘AIM: To investigate the role of sorafenib(SFN) in autophagy of hepatocellular carcinoma(HCC). We evaluated how SFN affects autophagy signaling pathway in human HCC cell lines. METHODS: Two different human HCC cell lines, Hep3 B and Huh7, were subjected to different concentrations of SFN. Cell viability and onset of apoptosis were determined with colorimetric assay and immunoblotting analysis, respectively. The changes in autophagy-related proteins, including LC3, ULK1, AMPK, and LKB, were determined with immunoblotting analysis in the presence or absence of SFN. To assess autophagic dynamics, autophagic flux was measured with chloroquine, a lysosomal inhibitor. The autophagic responsiveness between different HCC cell lines was compared under the autophagy enhancing conditions.RESULTS: Hep3 B cells were significantly more resistant to SFN than Huh7 cells. Immunoblotting analysis revealed a marked increase in SFN-mediated autophagy flux in Huh7 cells, which was, however, absent in Hep3 B cells. While both starvation and rapamycin enhanced autophagy in Huh7 cells, only rapamycin increased autophagy in Hep3 B cells. Immunoblotting analysis of autophagy initiation proteins showed that SFN substantially increased phosphorylation of AMPK and consequently autophagy in Huh7, but not in Hep3 B cells.CONCLUSION: The autophagic responsiveness to SFN is distinct between Hep3 B and Huh7 cells. Resistance of Hep3 B cells to SFN may be associated with altered autophagy signaling pathways. | Trevan D Fischer Jin-Hee Wang Adrian Vlada Jae-Sung Kim Kevin E Behrns | 2014 | World Journal of Hepatology2014，6，10： | 5 |
| 2 | Do not censor science in the name of bioseeurity 显示文摘 | Trevan T | 2012 | Nature2012，486，7403： | 1 |
| 3 | Immobilized Algae and Their Potential for Use as Biocatalysis显示文摘 | Trevan MD and Mak AL | 1988 | Trends & Biotechnol1988，6，3： | 1 |
| 4 | Purification and partial characterization of tomato extension peroxidase显示文摘 | Brownleader M Ahmed N Trevan M | | 0，，： | 1 |
| 5 | Do not censor science in the name of biosecurity 显示文摘 | Trevan T | 2012 | Nature2012，486，7403： | 1 |
| 6 | Na^+ ,K^+ -ATPase isozyme diversity; comparative biochemistry and physiological implications of novel functional interactions显示文摘 | Mobasheri A Avila J Cozar-Castellano I Brownleader M D Trevan M Francis M J | 2000 | Biosci Rep2000，20，： | 1 |
| 7 | Immobilized algae and their potential for use as biocatalysts 显示文摘 | Trevan M D Mak A L | 1988 | Trends Biotechnol1988，6，3： | 1 |
| 8 | Lipid keratopathy and atheromatosis in an SPF laboratory rabbit colony attrib- utable to diet显示文摘 | Sebesteny A Sheraidah GA Trevan D J | 1985 | Lab Anim1985，19，3： | 1 |
| 9 | 小麦种子中与抗赤霉病性相关的生化成分分析显示文摘 用FPLC方法分析了抗小麦赤霉病程度不同的8个小麦品种种子的水溶性组分。结果表明,所有小麦品种都有1个保留时间为9min的吸收峰(P9),且抗病品种中该吸收峰的高度明显高于感病品种。种子水溶液经过氧化氢温育处理后,P9明显升高,表明有大量的P9生成。因此,推测P9可能是1种与小麦抗赤霉病性相关的生化成分,在小麦抗赤霉病的过程中起作用。 | 林玲 STUART D.Thompson 陈怀谷 MICHAEL Trevan PRAKASH M.Dey ANGELO Pantelides 王裕中 史建荣 | 2003 | 江苏农业学报2003，19，1： | 1 |
| 10 | Purification and partial characterization of tomato extensin peroxidase 显示文摘 | Brownleader M D Ahmed N Trevan M | 1995 | Plant Physiology1995，109，： | 1 |
| 11 | A critical evaluation of the In capacity Status Scale显示文摘 | Mertin J Jones L Trevan R | 1984 | Acta Neurol Scand Suppl1984，101，： | 1 |
| 12 | The putative common mechanism for inactivation of alkaline phosphatase is oenzymes显示文摘 | Poltorak O M Chukhrai E S Kozlenkov A A Chaplin M F Trevan M D | 1999 | Journal of Molecular Catalysis B:Enzymatic1999，7，14： | 1 |
| 13 | Purification and partial characterization of tomato extensin peroxidase显示文摘 | Brownleader M D Ahmed N Trevan M | 1995 | Plant Physiology1995，109，： | 1 |
| 14 | Autophagy Suppresses Age-Dependent Ischemia and Reperfusion Injury in Livers of Mice显示文摘 | Jin–Hee Wang In–Sook Ahn Trevan D. Fischer Jae–Il Byeon William A. Dunn Kevin E. Behrns Christiaan Leeuwenburgh Jae–Sung Kim | 2011 | Gastroenterology2011，，6： | 1 |
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