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| 1 | Rapid mitogen-activated protein kinase by basic fibroblast growth factor in rat intestine after ischemia/reperfusion injury显示文摘AIM: Previous studies showed that exogenous basic fibroblast growth factor (bFGF or FGF-2) could improve physiological dysfunction after intestinal ischemia/reperfusion (I/R) injury. However, the mechanisms of this protective effect of bFGF are still unclear. The present study was to detect the effect of bFGF on the activities of mitogen-activated protein kinase (MAPK) signaling pathway in rat intestine after I/R injury, and to investigate the protective mechanisms of bFGF on intestinal ischemiain jury.METHODS: Rat intestinal I/R injury was produced by clamping the superior mesenteric artery (SMA) for 45 minutes and followed by reperfusion for 48 hours. Seventy-eight Wistar rats were used and divided randomly into sham-operated group (A), normal saline control group (B),bFGF antibody pre-treated group (C), and bFGF treated group (D). In group A, SMA was separated without occlusion. In groups B, C and D, SMA was separated and occluded for 45 minutes, then, released for reperfusion for 48 hours. After the animals were sacrificed, blood and tissue samples were taken from the intestine 45 minutes after ischemia in group A and 2, 6, 24, and 48 hours after reperfusion in the other groups. Phosphorylated forms of p42/p44 MAPK, p38 MAPK and stress activated protein kinase/C-Jun N-terminal kinase (SAPK/JNK) were measured by immunohistochemistry. Plasma levels of D-lactate were examined and histological changes were observed under the light microscope.RESULTS: Intestinal I/R injury induced the expression of p42/p44 MAPK, p38 MAPK, and SAPK/JNK pathways and exogenous bFGF stimulated the early activation of p42/p44 MAPK and p38 MAPK pathways. The expression of phosphorylated forms of p42/p44 MAPK was primarily localized in the nuclei of crypt cells and in the cytoplasm and nuclei of villus cells. The positive expression of p38 MAPK was localized mainly in the nuclei of crypt cells, very few in villus cells. The activities of p42/p44 MAPK and p38 MAPK peaked 6 hours after reperfusion in groups B and C,while SAPK/JNK peaked 24 hours after reperfusion. The activities of p42/p44 MAPK and p38 MAPK peaked 2 hours after reperfusion in group D and those of SAPK/JNK were not changed in group B. D-lactate levels and HE staining showed that the intestinal barrier was damaged severely 6 hours after reperfusion, however, histological structures were much improved 48 hours after reperfusion in group D than in the other groups.CONCLUSION: The results indicate that intestinal I/R injury stimulates the activities of MAPK pathways, and that p42/p44 MAPK and p38MAPK activities are necessary for the protective effect of exogenous bFGF on intestinal I/R injury.The protective effect of bFGF on intestinal dysfunction may be mediated by the early activation of p42/p44 MAPK and p38 MAPK signaling pathways. | Xiao-Bing Fu Yin-Hui Yang Tong-Zhu Sun Wei Chen Jun-You Li Zhi-Yong Sheng Wound Healing and Cell Biology Laboratory,Burns Institute,304 Hospital,Trauma Center of Postgraduate Medical College,Beijing 100037,China | 2003 | World Journal of Gastroenterology2003，9，6： | 11 |
| 2 | Activation of phosphorylating-p38 mitogen-activated protein kinase and its relationship with localization of intestinal stem cells in rats after ischemia-reperfusion injury显示文摘AIM: To investigate the expression of phosphorylating p38mitogen-activated protein kinase (MAPK) in rat small intestine after ischemia-reperfusion (I/R) insult and its relationship with the localization of intestinal stem cells.METHODS: Forty-eight Wistar rats were divided randomly into three groups, namely intestinal ischemia-reperfusion group (R), intestinal ischemia group (Ⅰ) and sham-operated control group (C). In group I, the animals were killed 45minutes after superior mesenteric artery (SMA) occlusion,while in group R the rats sustained SMA occlusion for 45 minutes and reperfusion for 2, 6, 12 or 24 hours respectively. In shamoperated control group, SMA was separated, but without occlusion. The activity of plasma diamine oxidase (DAO)was determined. Intestinal tissue samples were also taken for histological analysis and immunohistochemical analysis of MAPK p38 detection and intestinal stem cell localization.RESULTS: The changes in histological structure and plasma DAO levels indicated that the intestinal barrier was damaged after intestinal I/R injury. In group C and I, each crypt contained 5-6 p38 MAPK positive cells, which were mainly located in the lower region of the crypts. This was consistent with the distribution of intestinal stem cells. The presence of positive cells in crypts increased with the time of reperfusion and reached its peak at 12 hours after reperfusion (35.6%).CONCLUSION: After intestinal T/R injury, the expression of phosphorylating-p38 MAPK in small intestine increased with the duration of reperfusion, and its distribution coincided with that of intestinal stem cells and their daughter cells,indicating that phosphorylating-p38 might be a possible marker of intestinal stem cells. | Xiao-Bing Fu Feng Xing Yin-Hui Yang Tong-Zhu Sun Bao-Chen Guo Wound Healing and Cell Biology Laboratory,Institute of Burns,304 Hospital,Trauma Center of Postgraduate Medical College,Beijing 100037,China | 2003 | World Journal of Gastroenterology2003，9，9： | 10 |
| 3 | Spinal cord injury in the setting of traumatic thoracolumbar fracture is not reliably associated with increased risk of associated intra-abdominal injury following blunt trauma:An analysis of a National Trauma Registry database显示文摘Purpose:There is a common opinion that spinal fractures usually reflect the substantial impact of injuries and therefore may be used as a marker of significant associated injuries,specifically for intra-abdominal injury(IAI).The impact of concomitant spinal cord injury(SCI)with the risk of associated IAI has not been well clarified.The aim of this study was to evaluate the incidence and severity of IAIs in patients suffering from spinal fractures with or without SCI.Methods:A retrospective cohort study using the Israeli National Trauma Registry was conducted.Patients with thoracic,lumbar and thoracolumbar fractures resulting from blunt mechanisms of injury from January 1,1997 to December 31,2018 were examined,comparing the incidence,severity and mortality of IAIs in patients with or without SCI.The collected variables included age,gender,mechanism of injury,incidence and severity of the concomitant IAIs and pelvic fractures,abbreviated injury scale,injury severity score,and mortality.Statistical analysis was performed using GraphPad InStat?Version 3.10,with Chi-square test for independence and two sided Fisher’s exact probability test.Results:Review of the Israeli National Trauma Database revealed a total of 16,878 patients with spinal fractures.Combined thoracic and lumbar fractures were observed in 1272 patients(7.5%),isolated thoracic fractures in 4967 patients(29.4%)and isolated lumbar fractures in 10,639 patients(63.0%).The incidence of concomitant SCI was found in 4.95%(63/1272),7.65%(380/4967)and 2.50%(266/10639)of these patients,respectively.The overall mortality was 2.5%,proving higher among isolated thoracic fracture patient than among isolated lumbar fracture counterparts(11.3%vs.4.6%,p<0.001).Isolated thoracic fractures with SCI were significantly more likely to die than non-SCI counterparts(8.2%vs.3.1%,p<0.001).There were no differences in the incidence of IAIs between patients with or without SCI following thoracolumbar fractures overall or in isolated thoracic fractures;although isolated lumbar fractures patients with SCI were more likely to have renal(3.4%vs.1.6%,p=0.02)or bowel injuries(2.3%vs.1.0%,p=0.04)than the non-SCI counterparts.Conclusion:SCI in the setting of thoracolumbar fracture does not appear to be a marker for associated IAI.However,in a subset of isolated lumbar fractures,SCI patient is associated with increased risks for renal and bowel injury. | Veacheslav Zilbermints Yehuda Hershkovitz Kobi Peleg Joseph J.Dubose Adi Givon David Aranovich Mickey Dudkiewicz Israeli Trauma Group Boris Kessel | 2021 | Chinese Journal of Traumatology2021，24，3： | 9 |
| 4 | Analysis of incidence of traumatic brain injury in blunt trauma patients with Glasgow Coma Scale of 12 or less显示文摘 | Alexander Becket Kobi Peleg Oded Olsha Adi Givon Boris Kessel Israeli Trauma Group | 2018 | Chinese Journal of Traumatology2018，21，3： | 7 |
| 5 | Tuina Therapy of Lumbar Intervertebral Disc Protrusion显示文摘TuinaTherapyofLumbarIntervertebralDiscProtrusion￥JiangWeizhuang(InstituteofOrthoP./TraumaChinaAcademyofTraditionalChineseMedi... | Jiang Weizhuang(Institute of OrthoP. /Trauma China Academy of TraditionalChinese Medicine Beijing 100700, China) | 1995 | 中国针灸1995，15，S2： | 3 |
| 6 | Modulating autophagy:a strategy for cancer therapy显示文摘Autophagy is a process in which long-lived proteins,damaged cell organelles,and other cellular particles are sequestered and degraded.This process is important for maintaining the cellular microenvironment when the cell is under stress.Many studies have shown that autophagy plays a complex role in human diseases,especially in cancer,where it is known to have paradoxical effects.Namely,autophagy provides the energy for metabolism and tumor growth and leads to cell death that promotes tumor suppression.The link between autophagy and cancer is also evident in that some of the genes that regulate carcinogenesis,oncogenes and tumor suppressor genes,participate in or impact the autophagy process.Therefore,modulating autophagy will be a valuable topic for cancer therapy.Many studies have shown that autophagy can inhibit the tumor growth when autophagy modulators are combined with radiotherapy and/or chemotherapy.These findings suggest that autophagy may be a potent target for cancer therapy. | Jun-Lin Li1,Shao-Liang Han2 and Xia Fan3 1Department of General Surgery,The Central Hospital of Yongzhou City,Yongzhou,Hunan 425000,P.R.China 2Department of General Surgery,First Affiliated Hospital of Wenzhou Medical College,Wenzhou,Zhejiang 325000,P.R.China 3State Key Laboratory of Trauma,Burns,and Combined Injury Research Institute of Surgery,Daping Hospital,Third Military Medical University,Chongqing 400042,P.R.China. | 2011 | Chinese Journal of Cancer2011，30，10： | 3 |
| 7 | Nonunion following intramedullary nailing of the femur with and without reaming:results of a multicenter random ized clinical trial显示文摘 | Canadian Orthopaedic Trauma Society | | 0，，： | 1 |
| 8 | Guidelines for the management of severe traumatic brain injury 显示文摘 | Brain Trauma Foundation American Association of Neurological Surgeons Congress of Neurological Surgeons Joint Section on Neurotrauma and Critical Care | 2007 | J Neurotrauma2007，24，1： | 1 |
| 9 | Guidelines for the management of severe traumatic brain injury 显示文摘 | Brain Trauma Foundation American Association of Neurological Surgeons Congress of Neurological Surgeons | 2007 | J Neurotrauma2007，24，1： | 1 |
| 10 | Head trauma in China显示文摘 | Jiang JY Chinese Head Trauma Study Collaborators | 2013 | Injury2013，44，11： | 1 |
| 11 | Nonunion following intramedullary nailing of the femur with and without reaming:results of a mulitcenter randomized clinical trial显示文摘 | Canadian Orthopaedic Trauma Society | 2003 | J Bone Joint Surg(Am)2003，85，： | 1 |
| 12 | Intra-articular fractures of the distal tibia: the pilon fracture 显示文摘 | J Orthop Trauma Am J Orthop (Belle Bourne RB Rorabeck CH Macnab J | | J Trauma0，23，7： | 1 |
| 13 | Nonoperative treatment compared with plate fixation of displaced midshaft clavicular fractures,A muhicenter, randomized clinical trial显示文摘 | Canadian Orthopaedic Trauma Society | 2007 | J Bone Joint Surg(Am)2007，89，1： | 1 |
| 14 | Non- operative treatment compared with plate fixation of displaced midshaft clavicular fractures 显示文摘 | Altamini SA McKee MD Canadian orthopaedic trauma society | 2008 | J Bone Joint Surg Am2008，90，2： | 1 |
| 15 | Guide-lines for the management of severe traumatic brain injury显示文摘 | Brain Trauma Foundation American Association of Neuro-logical Surgeons Congress of Neurological Surgeons | 2007 | J Neurotrauma2007，24，： | 1 |
| 16 | Recommendations for intracranial pressure monitoring technology显示文摘 | The Brain Trauma Foundation The American Association of Neurological Surgeons The Joint Section on Neurotrauma and Critical Care | 2000 | J Neurotrauma2000，17，617： | 1 |
| 17 | Nonoperative treatment compared with plate fixation of displaced midshaft clavicular fractures:a multicenter randomized clinical trial显示文摘 | Canadian Orthopaedic Trauma Society | 2007 | J Bone Joint Surg Am2007，89，1： | 1 |
| 18 | Nonoperative treatment compared with plate fixation of displaced midshaft clavicular fractures 显示文摘 | Canadian Orthopaedic Trauma Society | 2007 | J Bone Joint Surg ( Am )2007，89，1： | 1 |
| 19 | Treatment of acute midshaft clavicle fractures: systematic review of 2 144 fractures:on behalf of the Evidence-Based Orthopaedic Trauma Working Group显示文摘 | Zlowodzki M Zelle B A Cole P A Jeray K McKee M D Evidence-Based Orthopaedic Trauma Working Group | 2005 | Jorthop Trauma2005，19，： | 1 |
| 20 | Guidelines for the management of severe traumatic brain injury显示文摘 | Brain trauma foundation American association of Neurological Surgeons Congress of Neurosurgical Surgeons | 2007 | J Neurotrauma2007，24，： | 1 |
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