4篇
您的检索式：作者名="Tim Muller"
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| 1 | Serological responses to Cryptosporidium antigens among women using riverbank-filtered water, conventionally filtered surface water and groundwater in Hungary 显示文摘 | Floyd Frost Tim Muller Gunther Craun | 2005 | Journal of Water and Heahh2005，，03： | 1 |
| 2 | Suction dressings to secure a dermal substitute显示文摘 | Winston McEwan Tim La H Brown Stephen M Mills Michael J Muller | 2003 | Burns2003，，： | 1 |
| 3 | Modeling Northern Peatland Decomposition and Peat Accumulation显示文摘 | Steve Frolking Nigel T. Roulet Tim R. Moore Pierre J. H. Richard Martin Lavoie Serge D. Muller | 2001 | Ecosystems2001，，5： | 1 |
| 4 | Systematic Cross-biospecimen Evaluation of DNA Extraction Kits for Long-and Short-read Multi-metagenomic Sequencing Studies显示文摘High-quality DNA extraction is a crucial step in metagenomic studies.Bias by different isolation kits impairs the comparison across datasets.A trending topic is,however,the analysis of multiple metagenomes from the same patients to draw a holistic picture of microbiota associated with diseases.We thus collected bile,stool,saliva,plaque,sputum,and conjunctival swab samples and performed DNA extraction with three commercial kits.For each combination of the specimen type and DNA extraction kit,20-gigabase(Gb)metagenomic data were generated using short-read sequencing.While profiles of the specimen types showed close proximity to each other,we observed notable differences in the alpha diversity and composition of the microbiota depending on the DNA extraction kits.No kit outperformed all selected kits on every specimen.We reached consistently good results using the Qiagen QiAamp DNA Microbiome Kit.Depending on the specimen,our data indicate that over 10 Gb of sequencing data are required to achieve sufficient resolution,but DNA-based identification is superior to identification by mass spectrometry.Finally,longread nanopore sequencing confirmed the results(correlation coefficient>0.98).Our results thus suggest using a strategy with only one kit for studies aiming for a direct comparison of multiple microbiotas from the same patients. | Jacqueline Rehner Georges Pierre Schmartz Laura Groeger Jan Dastbaz Nicole Ludwig Matthias Hannig Stefan Rupf Berthold Seitz Elias Flockerzi Tim Berger Matthias Christian Reichert Marcin Krawczyk Eckart Meese Christian Herr Robert Bals Soren L.Becker Andreas Keller Rolf Muller The IMAGINE Consortium | 2022 | Genomics, Proteomics & Bioinformatics2022，20，2： | 0 |
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