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| 1 | Interleukin-1α, 6 regulate the secretion of vascular endothelial growth factor A, C in pancreatic cancer显示文摘Vascular endothelial growth factor (VEGF, namely VEGF-A) is an angiogenic polypeptide and VEGF-C is a lymphangiogenic polypeptide that has been implicated in cancer growth, invasion and metastasis. Several cytokines and growth factors play an important part in cancer progression. These cytokines and growth factors are the principal mediators of cancer cells-stromal cell interaction , which is critical for invasion of cancer cells to the surrounding tissues and metastatic dissemination to distant organs. In this study, we studied VEGF-A, C expression in cultured human pancreatic cancer cell lines and whether the presence of VEGF-A, C in the cell lines is regulated by cytokines interleukin-lct (EL-1α), and interleukin-6 (IL-6). METHODS: We used Northern blot and Western blot methods to analyze expression of the gene and protein of VEGF-A, C in all 6 tested cell lines (ASPC-1, CAPAN-1, MIA-PaCa-2, PANC-1, COLO-357 and T3M4) respectively. To analyze what is the regulator for this VEGF-A, C expression in pancreatic cancer,we used the reverse transcription -polymerase chain reaction (RT-PCR) method to analyze VEGF-A, C expression in cultured human pancreatic cancer cell lines (CAPAN-1 and COLO-357) under the stimulation with IL-1α (10μg/L) or IL-6 (100 μg/L). RESULTS:Northern blot analysis revealed the presence of the 4.1-kb VEGF-A mRNA transcript and 2.4-kb VEGF-C mRNA transcript in all 6 tested cell lines. Immunoblotting with highly specific anti-VEGF-A, anti-VEGF-C antibody revealed the presence of a molecular weight of 43-kDa VEGF-A protein and 55-kDa VEGF-C protein in all the cell lines. RT-PCR analysis revealed the levels of the VEGF-A and VEGF-C gene were 1-2 fold and a 1-fold increase in the COLO-357 cell line by stimulation with IL-la, however, no effect was found in the CAPAN-1 cell line. The levels of the VEGF-A and VEGF-C gene were 2-5 fold and a 1-fold increase in the CAPAN-1 cell line by stimulation with IL-6, but, no effect was found in the COLO-357 cell line. CONCLUSION:These findings suggested that the expression of VEGF-A, C and their regulation by IL-1α, IL-6 in pancreatic cancer contributes to the lymphatic and distant metastasis and the disease progression. | Department of Hepatobiliary Surgery (Tang RF, Zhang FR, Peng L, Wang SX, Xiao Y and Zhang M) and Department of Dermatology (Wang SX), 4th Hospital, Hebei Medical University, Shijiazhuang 050011, China | 2005 | Hepatobiliary & Pancreatic Diseases International2005，4，3： | 6 |
| 2 | The solar wind plasma upstream of Mars observed by Tianwen-1:Comparison with Mars Express and MAVEN显示文摘On the great journey to Mars,China’s first planetary exploration mission,the Tianwen-1 came within 26 million kilometers of Mars from 31 October 2020 to 25 January 2021 and was getting closer to its destination,the red planet,in search of answers to the cataclysmic climate change that occurred in Martian history.Both the escape of the Martian atmosphere and the loss of surface water were firmly influenced by solar activities.Tianwen-1 provided a unique chance to depict the solar wind streams between Earth and Mars during the minimum of Solar Cycle 25.During the three-month cruise phase of Tianwen-1,the solar wind flows were successively observed at Earth,Tianwen-1,and Mars.After the field of view correction and noise reduction,the solar wind velocity and density measured by Tianwen-1 show good agreement with those at Earth and Mars.The results indicate that the performance of the ion analyzer onboard the Tianwen-1 orbiter is reliable and stable.It is worth looking forward to the joint observations of ion escape with other Mars probes in the following Martian years. | Kai FAN Limei YAN Yong WEI Aibing ZHANG Linggao KONG Markus FRÄNZ Fei HE Lihui CHAI Chongjing YUAN Yuqi WANG Jun ZHONG Zhaojin RONG Zhonghua YAO Yongxin PAN Jun CUI Jiansen HE Wenya LI Binbin TANG Chi WANG | 2022 | Science China Earth Sciences2022，65，4： | 2 |
| 3 | Engineering of dendritic dopant-free hole transport molecules:enabling ultrahigh fill factor in perovskite solar cells with optimized dendron construction显示文摘Developing dopant-free hole-transporting materials(HTMs)for high-performance perovskite solar cells(PVSCs)has been a very active research topic in recent years since HTMs play a critical role in optimizing interfacial charge carrier kinetics and in turn determining device performance.Here,a novel dendritic engineering strategy is first utilized to design HTMs with a D-A type molecular framework,and diphenylamine and/or carbazole is selected as the building block for constructing dendrons.All HTMs show good thermal stability and excellent film morphology,and the key optoelectronic properties could be fine-tuned by varying the dendron structure.Among them,MPA-Cz-BTI and MCz-Cz-BTI exhibit an improved interfacial contact with the perovskite active layer,and non-radiative recombination loss and charge transport loss can be effectively suppressed.Consequently,high power conversion efficiencies(PCEs)of 20.8%and 21.35%are achieved for MPA-Cz-BTI and MCz-Cz-BTI based devices,respectively,accompanied by excellent long-term storage stability.More encouragingly,ultrahigh fill factors of 85.2%and 83.5%are recorded for both devices,which are among the highest values reported to date.This work demonstrates the great potential of dendritic materials as a new type of dopant-free HTMs for high-performance PVSCs with excellent FF. | Wei Chen Yang Wang Bin Liu Yajun Gao Ziang Wu Yongqiang Shi Yumin Tang Kun Yang Yujie Zhang Weipeng Sun Xiyuan Feng Frédéric Laquai Han Young Woo Aleksandra B.Djurisic Xugang Guo Zhubing He | 2021 | Science China Chemistry2021，64，1： | 2 |
| 4 | Metabotropic Glutamate Receptor 2/3 in the Hippocampus of Patients with Mesiel Temporal Lobe Epilepsy, and of Rats and Mice after Pilocarpine-lndueed Status Epilepticus 显示文摘 | Tang FR Cha SC Chen PM | 2004 | Epilepsy Res2004，59，23： | 1 |
| 5 | Multiple molecular penumbras after focal cerebral ischemia显示文摘 | Sharp FR Lu A Tang Y | 2000 | J Cereb Blood Flow Metab2000，20，： | 1 |
| 6 | Multiple molecular penumbras after focal cerebral ischemia 显示文摘 | Sharp FR Lu A Tang Y | 2000 | J Cereb Blood Flow Metab2000，20，77： | 1 |
| 7 | Multiple molecular penumbras after focal cerebral ischemia显示文摘 | Sharp FR Lu A Tang Y | 2000 | J Cereb Blood Flow Metab2000，20，7： | 1 |
| 8 | Multiple molecular penumbras after focal cerebral ischemia显示文摘 | Sharp FR Lu A Tang Y | | 0，，： | 1 |
| 9 | Multiple molecular penum- bras after focal cerebral ischemia 显示文摘 | Sharp FR Lu A Tang Y | 2000 | J Cereb Blood Flow Metab2000，20，7： | 1 |
| 10 | Hypermethylation of the death-associated protein(DAP) kinase promoter and aggressiveness in stage I non-small-cell lung cancer 显示文摘 | Tang X Khuri FR Lee JJ | 2000 | J Natl Cancer Inst2000，92，： | 1 |
| 11 | Hypermethylation of the death-associated protein (DAP)kinase promoter and aggressiveness in stage I non-small-cell lung cancer显示文摘 | Tang X Khuri FR Lee JJ | 2000 | J Natl Cancer Inst2000，92，： | 1 |
| 12 | Hypermethylation of the death-associated protein (DAP) kinase promoter and aggressiveness in stage I non-small-cell lung cancer显示文摘 | Tang X Khuri FR Lee JJ | 2000 | J Natl Cancer Inst2000，92，18： | 1 |
| 13 | Multiple molecular penumbras af- ter focal cerebral ischemia显示文摘 | Sharp FR Lu A Tang Y | 2000 | J Cereb Blood Flow Metab2000，20，10： | 1 |
| 14 | Multiple molecular penumbras after focal cerebral ischemia显示文摘 | Lu A Tang Y | 2000 | J Cereb Blood Flow Metab2000，20，： | 1 |
| 15 | Reduction of infarct size by orally administered des-aspartate-angiotensin i in the ischemic reperfused rat heart显示文摘 | Wen Q Sim MK Tang FR | 2004 | Regul Pept2004，120，： | 1 |
| 16 | Effects of des-aspartate-angiotensin I on neointima growth and cardiovascular hypertrophy显示文摘 | Sim MK Tang FR Xu XG | 2004 | Regul Pept2004，117，： | 1 |
| 17 | Multiple molecular penumbras after focal cerebral ischemia显示文摘 | Sharp FR Lu A Tang Y | 2000 | J Cereb Blood Flow Metab2000，20，7： | 1 |
| 18 | MuhiPle molecular Penumbras after fcealcerebral ischemia显示文摘 | SharP FR Lu A Tang Y | 2000 | J Cereb Blood Flow Metab2000，20，7： | 1 |
| 19 | Expression of metabotropic glutamate receptor 8 in the rat hippocampus after pilocarpine induced status epilepticus显示文摘 | Lee WL Yang J | 2001 | Neurosci Lett2001，300，： | 1 |
| 20 | Activation and sensitization of C and Aδ afferent fibers mediated by P2X receptors 显示文摘 | Zhang Q Zhao Y Guo Y Cao DY Tang XD Tian YL Yao FR Wang HS | 2006 | Brain Res2006，1102，： | 1 |
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