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| 1 | Sieving characteristics of cytokine-and peroxide-induced epithelial barrier leak:Inhibition by berberine显示文摘AIM:To study whether the inflammatory bowel disease(IBD)colon which exhibits varying severity and cytokine levels across its mucosa create varying types of transepithelial leak.METHODS:We examined the effects of tumor necrosis factor-α(TNF-α),interferon-γ(IFN-γ),interleukin-1-β(IL1β)and hydrogen peroxide(H_2O_2)-singly and in combinations-on barrier function of CACO-2 cell layers.Our focus was on the type(not simply the magnitude)of transepithelial leak generated by these agents as measured by transepithelial electrical resistance(TER)and transepithelial flux of ^(14)C-D-mannitol,3H-Lactulose and ^(14)C-Polyethylene glycol as radiolabeled probe molecules.The isoquinoline alkaloid,berberine,was then examined for its ability to reduce specific types of transepithelial leak.RESULTS:Exposure to TNF-α alone(200 ng/mL;48 h)induced a 50% decrease in TER,i.e.,increased leak of Na^+ and Cl--with only a marginal but statistically significant increase in transepithelial leak of ^(14)C-mannitol(Jm).Exposure to TNF-α + IFN-γ(200 ng/mL;48 h)+ IL1β(50 ng/mL;48 h)did not increase the TER change(from TNF-α alone),but there was now a 100% increase in J_m.There however was no increase in transepithelial leak of two larger probe molecules,~3H-lactulose and ^(14)C-polyethylene glycol(PEG).However,exposure to TNF-α + IFN-γ + IL1β followed by a 5 h exposure to2 mmol/L H_2O_2 resulted in a 500% increase in ^(14)C-PEG leak as well as leak to the luminal mitogen,epidermal growth factor.CONCLUSION:This model of graded transepithelial leak is useful in evaluating therapeutic agents reducing IBD morbidity by reducing barrier leak to various luminal substances. | Katherine M DiGuilio Christina M Mercogliano Jillian Born Brendan Ferraro Julie To Brittany Mixson Allison Smith Mary Carmen Valenzano James M Mullin | 2016 | World Journal of Gastrointestinal Pathophysiology2016，7，2： | 6 |
| 2 | Autophagosome-associated variant isoforms of cytosolic enzymes显示文摘 | Fengsrud M Raiborg C Berg TO Strmhaug PE Ueno T Erichsen ES Seglen PO | 2000 | Biochem J2000，352，3： | 1 |
| 3 | Stress henodynamic and inhaled and intravenous anesthetic laparoscopic chloecystectomy显示文摘 | Grazida E Elena G Gobbo immunological techniques for Esp Anestesid responses to video-assisted M | 2005 | Rev Reanim2005，52，4： | 1 |
| 4 | Effects of three dif-ferent dose regimens of magnesium on propofol requirements,haemodynamic variables and postoperative pain relief in gynecological surgery显示文摘 | Seyhan TO Tugrul M Sungur MO | 2006 | Br J Anaesth2006，96，2： | 1 |
| 5 | Early interleukin 4-dependent response can induce airway hyperreacfivity before development of airway inflammation in a mouse model of asthma显示文摘 | To Y Dohi M Tanaka R | 2001 | Lab Invest2001，81，10： | 1 |
| 6 | Characterisation of gas phase organic emissions from hot cooking oil in commercial kitchens 显示文摘 | To W M Yeung L L Chao C Y H | 2002 | Indoor Built Environment2002，9，： | 1 |
| 7 | A Simple Method for Designation of Urban Ventilation Corridors and Its Application to Urban Heat Island Analysis显示文摘 | Wong M S Nichol J E To P H | | 0，，08： | 1 |
| 8 | The use of nitrite in meat显示文摘 | Cassens R G /to T Lee M el al | 1978 | Bio- science1978，28，10： | 1 |
| 9 | Proteomic identification of Helicobacter pylori-associated gastroduodenal disease: Challenges and opportunities 显示文摘 | Wu M S ChowLP Lin J T biomarkers related to | 2008 | J Gastroenterol Hepatol2008，23，11： | 1 |
| 10 | Quantification of the superior lateral pterygoid insertion on TMJ compeonents显示文摘 | Dusek TO Kiely M | 1991 | J Dent Res1991，7042，： | 1 |
| 11 | Impact of Pulsed electric field treatment on the recovery and quality of plant oils 显示文摘 | Guder jan M To P S Angersbach A | 2004 | Journal of Food Engineering2004，26，： | 1 |
| 12 | Intraocular pressure elevation following triamcinolone acetonide administration as related to administration routes 显示文摘 | Hirano Y Nozaki M | 2009 | Jpn J Ophthalmol2009，53，5： | 1 |
| 13 | Bone mineralization defects and vitamin D deficiency:histomorphometric analysis of iliac crest bone biopsies and circulating 25-hydroxyvitamin D in 675 patients显示文摘 | Priemel M Von Domarus C Klatte TO | 2010 | J Bone Miner Res2010，25，： | 1 |
| 14 | Profiling YB-1target genes uncovers a new mechanism for MET receptor regulation in normal and malignant human mammary cells显示文摘 | Finkbeiner M R Astanehe A To K | 2009 | Oncogene2009，28，11： | 1 |
| 15 | Generation of wind loads on a horizontal grandstand roof of large aspect ratio显示文摘 | Lam K M To A P | 1995 | Journal of Wind Engineering and Industrial Aerodynamics1995，5455，： | 1 |
| 16 | Reducing failed extubations in the intensive care unit显示文摘 | Pronovost PJ Jenckes M To M | 2002 | Jt comm J Qual lmprove2002，28，11： | 1 |
| 17 | Spontaneous clearance of childhood hepatitis C virus infection显示文摘 | Yeung L T To T King S M | 2007 | J Viral Hepat2007，14，11： | 1 |
| 18 | Lysozyme strain Sterne in as a barrier liquid eggwhite, milk and beef 显示文摘 | Sung K Khan S A Nawaz M to growth of Bacillus anthracis S | 2011 | Food Microbiology2011，28，6： | 1 |
| 19 | Surgical management of long bone pseu- darthrosis with severe limb length discrepancy in osteogenesis imperfecta显示文摘 | To M Gupta V Chow W | 2013 | JPediatrOrthopB2013，22，1： | 1 |
| 20 | Infection rates following initial cerebrospinal fluid shunt placement across pediatric hospitals in the United States显示文摘 | Simon TO Hall M Riva-Cambrin 1 | 2009 | 1 Neurosurg Pediatr2009，4，2： | 1 |
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