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| 1 | Hepatogenic differentiation of human mesenchymal stem cells from adipose tissue in comparison with bone marrow mesenchymal stem cells显示文摘AIM: To investigate and compare the hepatogenic transdifferentiation of adipose tissue-derived stem cells (ADSC) and bone marrow-derived mesenchymal stem cells (BMSC) in vitro. Transdifferentiation of BMSC into hepatic cells in vivo has been described. Adipose tissue represents an accessible source of ADSC, with similar characteristics to BMSC.METHODS: BMSCs were obtained from patients under-going total hip arthroplasty and ADSC from human adi-pose tissue obtained from lipectomy. Cells were grown in medium containing 15% human serum. Cultures were serum deprived for 2 d before cultivating under similar pro-hepatogenic conditions to those of liver develop-ment using a 2-step protocol with sequential addition of growth factors, cytokines and hormones. Hepatic differ-entiation was RT-PCR-assessed and liver-marker genes were immunohistochemically analysed. RESULTS: BMSC and ADSC exhibited a fibroblastic mor-phology that changed to a polygonal shape when cells differentiated. Expression of stem cell marker Thy1 de-creased in differentiated ADSC and BMSC. However, the expression of the hepatic markers, albumin and CYPs increased to a similar extent in differentiated BMSC and ADSC. Hepatic gene activation could be attributed to in-creased liver-enriched transcription factors (C/EBPβ and HNF4α), as demonstrated by adenoviral expression vec-tors.CONCLUSION: Mesenchymal stem cells can be induced to hepatogenic transdifferentiation in vitro. ADSCs have a similar hepatogenic differentiation potential to BMSC, but a longer culture period and higher proliferation ca-pacity. Therefore, adipose tissue may be an ideal source of large amounts of autologous stem cells, and may become an alternative for hepatocyte regeneration, liver cell transplantation or preclinical drug testing. | Raquel Taléns-Visconti Ana Bonora Ramiro Jover Vicente Mirabet Francisco Carbonell José Vicente Castell María José Gómez-Lechón | 2006 | World Journal of Gastroenterology2006，12，36： | 35 |
| 2 | Nebulized 3% Hypertonic saline solution treatment in ambulatory children with viral, bronchiolitis decreases symptoms显示文摘 | Sarrell EM Tal G Witzling M | 2009 | Chest2009，122，1： | 1 |
| 3 | Biodegradable microcarriers as cell delivery vehicle for in vivo transplantation and magnetic resonance monitoring显示文摘 | Lovati AB Vianello E Talò G | | 0，，2： | 1 |
| 4 | Nebulised 3% hypertonic saline solution treatment in hospitalized infants with viral bronchiolitis显示文摘 | Mandelberg A Tal G Witzling M | 2003 | Chest2003，123，4： | 1 |
| 5 | Multilevel sensitive reconstruction of polyhedral surfaces from parallel slice 显示文摘 | Barequet G Shapiro D Tal A | 2000 | The Visual Comput- er2000，16，： | 1 |
| 6 | Deferred, self-organizing BSP trees 显示文摘 | Ar S Montag G Tal A | 2002 | Computer Graphics Forum2002，21，3： | 1 |
| 7 | Heat shock protein 60 activates cytokine-associated negative regulator suppressor of cytokine signaling 3 in T cells:effects on signaling,chemotaxis,and inflammation显示文摘 | Zanin-Zhorov A Tal G Shivtiel S | | 0，，： | 1 |
| 8 | Multilevel sensitive reconstruction of polyhedral surfaces from parallel slices 显示文摘 | BAREQUET G SHAPIRO D TAL A | 2000 | The Visual Computer2000，16，2： | 1 |
| 9 | Glutathione conjugation: a detoxification pathway for fenoxapropethyl in barley, crabgrass, oat and wheat显示文摘 | TAL J A ROMANO M L STEPHENSON G R | 1993 | Pestic Biochem Physiol1993，46，： | 1 |
| 10 | Heat shock protein 60 enhances CD4+CD25+ regulatory T cell function via innate TLR2 signaling 显示文摘 | Zznin-Zhorov A Cahalon L Tal G | 2006 | J Clin Invest2006，116，7： | 1 |
| 11 | Extra-territorial pain in rats with a peripheral mononeuropathy: mechano-hyperalgesia and mechano-allodynia in the territory of an uninjured nerve显示文摘 | Tal M Bennett G J | 1994 | Pain1994，57，： | 1 |
| 12 | Nebulized 3%hypertonic saline solution treatment in ambulatory children with viral bronchiolitis decreases symptoms显示文摘 | Sarrell EM Tal G Witzling M | 2002 | Chest2002，122，6： | 1 |
| 13 | Heat Shock protein 60 en- hances CD4+ CD25 + regulatory T cell function via innate TLR2 signaling 显示文摘 | Zanin-Zhoror A Cahalon L Tal G | 2006 | J chin Invest2006，116，7： | 1 |
| 14 | Multilevel Sensitive Reconstruction of Polyhedral Surface from Parallel Slices显示文摘 | Barequet G Shapiro D Tal A | 2000 | The Visual Computer2000，16，： | 1 |
| 15 | Non -enzymatic conjugation of fenoxaprop - ethyl with glutathione and eysteine in several grass spe- eies显示文摘 | Tal J A Hall J C Stephenson G R | 1995 | Weed Research1995，35，3： | 1 |
| 16 | Virtual machine monitors: Current technology and future trends 显示文摘 | MENDEL R TAL G | 2005 | IEEE Computer2005，38，5： | 1 |
| 17 | Nebulized 3% Hypertonic saline solution treatment in ambulatory children with viral bronchiolitis decreases symptoms显示文摘 | Sarre11 E M Tal G Witzling M | 2002 | Chest2002，122，1： | 1 |
| 18 | Nebulised 3% hypertonic saline solution treatment in hospitalized infants with viral bronchiolitis显示文摘 | Tal G Witzling M | 2003 | Chest2003，123，4： | 1 |
| 19 | Hypertonic saline/epinephrine treatment in hospitalized infants with viral bronchiolitis reduces hospitalization stay: 2 years experience 显示文摘 | Tal G Ceser K Oron A | 2006 | Isr Med Assoc J2006，8，3： | 1 |
| 20 | Heteretopic pregnancy after ovula- tion induction and assisted reproductive technologies:a literature review from 1971 to 1993显示文摘 | Tal J Haddad S Nina G | 1996 | Fertility Sterility1996，，66： | 1 |
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