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| 1 | Scutellarin protects oxygen/glucose-deprived astrocytes and reduces focal cerebral ischemic injury显示文摘Scutellarin, a bioactive flavone isolated from Scutellaria baicalensis, has anti-inflammatory, anti-neurotoxic, anti-apoptotic and anti-oxidative effects and has been used to treat cardiovascular and cerebrovascular diseases in China. However, the mechanisms by which scutellarin mediates neuroprotection in cerebral ischemia remain unclear. The interaction between scutellarin and nicotinamide adenine dinucleotide phosphate oxidase 2(NOX2) was assessed by molecular docking study, which showed that scutellarin selectively binds to NOX2 with high affinity. Cultures of primary astrocytes isolated from the cerebral cortex of neonatal Sprague-Dawley rats were pretreated with 2, 10 or 50 μM scutellarin for 30 minutes. The astrocytes were then subjected to oxygen/glucose deprivation by incubation for 2 hours in glucose-free Dulbecco's modified Eagle's medium in a 95% N2/5% CO_2 incubator, followed by simulated reperfusion for 22 hours. Cell viability was assessed by cell counting kit-8 assay. Expression levels of NOX2, connexin 43 and caspase-3 were assessed by western blot assay. Reactive oxygen species were measured spectrophotometrically. Pretreatment with 10 or 50 μM scutellarin substantially increased viability, reduced the expression of NOX2 and caspase-3, increased the expression of connexin 43, and diminished the levels of reactive oxygen species in astrocytes subjected to ischemia-reperfusion. We also assessed the effects of scutellarin in vivo in the rat transient middle cerebral artery occlusion model of cerebral ischemia-reperfusion injury. Rats were given intraperitoneal injection of 100 mg/kg scutellarin 2 hours before surgery. The Bederson scale was used to assess neurological deficit, and 2,3,5-triphenyltetrazolium chloride staining was used to measure infarct size. Western blot assay was used to assess expression of NOX2 and connexin 43 in brain tissue. Enzyme-linked immunosorbent assay was used to detect 8-hydroxydeoxyguanosine(8-OHd G), 4-hydroxy-2-nonenal(4-HNE) and 3-nitrotyrosin(3-NT) in brain tissue. Immunofluorescence double staining was used to determine the co-expression of caspase-3 and Neu N. Pretreatment with scutellarin improved the neurological function of rats with focal cerebral ischemia, reduced infarct size, diminished the expression of NOX2, reduced levels of 8-OHd G, 4-HNE and 3-NT, and reduced the number of cells co-expressing caspase-3 and Neu N in the injured brain tissue. Furthermore, we examined the effect of the NOX2 inhibitor apocynin. Apocynin substantially increased connexin 43 expression in vivo and in vitro. Collectively, our findings suggest that scutellarin protects against ischemic injury in vitro and in vivo by downregulating NOX2, upregulating connexin 43, decreasing oxidative damage, and reducing apoptotic cell death. | Jing-Bo Sun Yan Li Ye-Feng Cai Yan Huang Shu Liu Patrick KK Yeung Min-Zhen Deng Guang-Shun Sun Prince LM Zilundu Qian-Sheng Hu Rui-Xin An Li-Hua Zhou Li-Xin Wang Xiao Cheng | 2018 | Neural Regeneration Research2018，13，8： | 16 |
| 2 | 急性缺血性脑卒中后低脉压与不良预后相关显示文摘众所周知,脉压与心脏功能、动脉僵硬度、体液状态和血管事件有关。该研究旨在探讨基于中国台湾地区脑卒中登记资料的急性缺血性脑卒中(acute ischemic stroke,AIS)患者入院时脉压的预后作用。 | 刘莉 叶鹏 Tang SC Yin JH Liu CH Sun MH Lee JT Sun Y Hsu CS Sun MC Lin CH Chen CH Lien LM Muo CH Jeng JS Hsu CY | 2017 | 中华高血压杂志2017，25，7： | 5 |
| 3 | Glucocorticoids upregulate transepithelial electrical resistance and expression of tight junction-related protein in human trabecular meshwork cells显示文摘 | ZHUO Ye-hong, HUANG Ya-lin, WEI Yan-tao, LING Yun-lan, LIN Ming-kai and GE Jian Key Laboratory of Ophthalmology, Ministry of Education Zhongshan Ophtha lm ic Center , Sun Yat-sen University, Guangzhou 510060, China | 2005 | Chinese Medical Journal2005，，20： | 4 |
| 4 | European Polymer Journal显示文摘 | Tang EJ Liu H Sun LM | 2007 | (10):4 210-4 2182007，，10： | 1 |
| 5 | Increased risk of cancer forpatients with ankylosing spondylitis : a nationwide population-basedretrospective cohort study 显示文摘 | Sun LM Muo CH Liang JA | 2014 | Scand J Rheumatol2014，43，4： | 1 |
| 6 | An enterovirus 71 epidem- ic in Guangdong Province of China, 2008 : epidemiological, clini- cal, and virogenic manifestations 显示文摘 | Sun LM Zheng HY Zheng HZ | 2011 | Jpn J Infect Dis2011，64，1： | 1 |
| 7 | Activation of Akt and eIF4E survival pathways by rapamycin-mediated mammalian target of rapamycin inhibition显示文摘 | Sun SY Rosenberg LM Wang X | 2005 | Cancer Res2005，65，16： | 1 |
| 8 | Role of IRS-2 in insulin and cytokine signaling显示文摘 | Sun XJ Wang LM Zhang Y | 1995 | Nature1995，377，： | 1 |
| 9 | Purification and bioactivity of sulphated polysaccharide conjugate ACP I from viscera of abalone Haliotis discus hannai Ino 显示文摘 | Sun LM Zhu BW Li DM | 2010 | Food and Agricultural Immunology2010，21，： | 1 |
| 10 | Phase 2 trial of mifepristone (RU-486) in advanced or recurrent endometrioid adenocarcinoma or low-grade endometrial stromal sarcoma显示文摘 | Ramondetta LM Johnson A J Sun CC | 2009 | Cancer2009，115，9： | 1 |
| 11 | A prospective cohort study to compare treatment results between 2 fractionation schedules ofhigh-dose-rate intracavitary brachytherapy (HDR-ICBT) in pa- tients with cervical cancer显示文摘 | Huang EY Sun LM Lin H | 2013 | lnt J Radiat Oncol Biol Phys2013，85，1： | 1 |
| 12 | Mucosal antibody responses in experimental hookworm infection显示文摘 | Bungiro RD Jr Sun T Harrison LM et ol | 2008 | Parasit Immunol2008，30，5： | 1 |
| 13 | Human adipose tissue-derived stem cells differentiate into endothelial cells in vitro and improve postnatal neovascularization in vivo显示文摘 | Cao Y Sun Z Liao LM | 2005 | Biochem Biophys Res Commun2005，332，： | 1 |
| 14 | Role of IRS-2 in insulin and cytokine signalling显示文摘 | Sun XJ Wang LM Zhang Y | 1995 | Nature1995，377，6545： | 1 |
| 15 | Characterization of hunran cytochrome P450 isoforms involved in the metabolism of 7-epi-paclitaxel显示文摘 | Zhang YY Iin Y Zhang JW Ge GB Wang LM Sun J | 2009 | Xenobiotica2009，39，4： | 1 |
| 16 | ROS1 Immunohistoehemistry for Detection of ROS1-Rearranged Lung Adenocarcinomas 显示文摘 | Sholl LM Sun H Butaney M | 2013 | Am J Surg Pathol2013，37，9： | 1 |
| 17 | Purification and partial bioactivity in vitro of polysaceharides from spomphyll of Undala pinnatifida 显示文摘 | Hang Q Yang DY Sun LM | 2012 | J F Agri Envi2012，10，3： | 1 |
| 18 | A prospective study to assess the bladder distension effects on dosimetry in intracavitary braehytherapy of cervical cancer via computed tomography assisted teehniques显示文摘 | Sun LM Huang HY Huang EY | 2005 | Radiother Oncol2005，77，1： | 1 |
| 19 | One step synthesis of dendrit ic gold nanoflowers with high sur{ace-enhanced Raman scattering (SERS) properties显示文摘 | Yi SJ Sun LM Lenaghan SC | 2013 | RSC Advances2013，3，10： | 1 |
| 20 | Activation of Akt and eIF4E survival pathways by Rapamyeinmediated mammalian target of Rapamycin inhibition 显示文摘 | Sun SY Rosenberg LM Wang X | 2005 | Cancer Res2005，65，16： | 1 |
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