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| 1 | The Chrysanthemum nankingense Genome Provides Insights into the Evolution and Diversification of Chrysanthemum Flowers and Medicinal Traits显示文摘The Asteraceae (Compositae),a large plant family of approximately 24 000-35 000 species,accounts for^10% of all angiosperm species and contributes a lot to plant diversity.The most representative members of the Asteraceae are the economically important chrysanthemums (Chrysanthemum L.)that diversified through reticulate evolution.Biodiversity is typically created by multiple evolutionary mechanisms such as wholegenome duplication 0NGD)or polyploidization and locally repetitive genome expansion.However,the lack of genomic data from chrysanthemum species has prevented an in-depth analysis of the evolutionary mechanisms involved in their diversification.Here,we used Oxford Nanopore long-read technologyto sequence the diploid Chrysanthemum nankingense genome,which represents one of the progenitor genomes of domesticated chrysanthemums.Our analysis revealed that the evolution of the C.nankingense genome was driven by bursts of repetitive element expansion and WGD events including a recentWGD that distinguishes chrysanthemum from sunflower,which diverged from chrysanthemum approximately 38.8 million years ago.Variations of ornamental and medicinal traits in chrysanthemums are linked to the expansion of candidate gene families by duplication events including paralogous gene duplication.Collectively,our study of the assembled reference genome offers new knowledge and resources to dissect the history and pattern of evolution and diversification of chrysanthemum plants,and also to accelerate their breeding and improvement. | Chi Song Yifei Liu Aiping Song Gangqiang Dong Hongbo Zhao Wei Sun Shyam Ramakrishnan Ying Wang Shuaibin Wang Tingzhao Li Yan Niu Jiafu Jiang Bin Dong Ye Xia Sumei Chen Zhigang Hu Fadi Chen Shilin Chen | 2018 | Molecular Plant2018，11，12： | 24 |
| 2 | Effects of dietary supplementation of Artemisia argyi aqueous extract on antioxidant indexes of small intestine in broilers显示文摘The present study was conducted to investigate the effect of Artemisia argyi aqueous extract(AAE) on antioxidant indexes in the small intestine. A total of 192 Arbor Acre broiler chickens(one-day-old) were randomly divided into 4 treatments with 6 replicates of 8 chickens. These 4 diets were formulated by adding 0, 500,1,000 and 2,000 mg/kg AAE to the basal diet. The results showed as follows: 1) compared with the control, the total antioxidant capacity(T-AOC) in ileum for the 2,000 mg/kg treatment group was significantly increased at 21 days of age(P < 0.05); the T-AOC levels in jejunum and ileum were significantly increased in broilers supplemented with 500 mg/kg AAE at 42 days of age(P < 0.05), and the T-AOC levels in jejunum and ileum were significantly improved in 1,000 mg/kg treatment group(P < 0.01), 2) At 21 days of age, supplementation of 500 mg/kg AAE significantly increased the catalase(CAT) activity of small intestine, and the glutathione peroxidase(GSH-Px) activity of jejunum was improved(P < 0.01), meanwhile, the GSH-Px activity of duodenum and the total superoxide dismutase(T-SOD) activity of duodenum and jejunum were significantly higher than those of the control group(P < 0.05); supplementation of 1,000 mg/kg AAE significantly increased the CAT activity of duodenum and ileum and the GSH-Px activity of duodenum and jejunum(P < 0.05), and the ileum GSH-Px activity was significantly increased(P < 0.01); supplementation of 2,000 mg/kg AAE significantly increased the CAT activity of duodenum and ileum(P < 0.05). At 42 days of age, supplementation of 500 mg/kg AAE significantly increased the GSH-Px activity of ileum and the T-SOD activity of duodenum(P < 0.05),meanwhile, the T-SOD activity of jejunum was significantly increased(P < 0.01); supplementation of1,000 mg/kg AAE significantly increased the CAT activity of jejunum and the T-SOD activity of ileum(P < 0.01), and the GSH-Px activity of jejunum was significantly increased(P < 0.05); supplementation of2,000 mg/kg AAE significantly increased the T-SOD activity of ileum(P < 0.05), but significantly decreased the CAT activity of ileum and the GSH-Px activity of jejunum(P < 0.05). 3) The malondialdehyde(MDA) levels of 3 AAE supplementation groups were significantly decreased at 21 and 42 days of age(P < 0.05). The results suggested that dietary supplementation with AAE could improve the antioxidative capacity of small intestine in broilers. | Fei Zhao Binlin Shi Dengsheng Sun Hongyan Chen Manman Tong Pengfei Zhang Xiaoyu Guo Sumei Yan | 2016 | Animal Nutrition2016，，3： | 18 |
| 3 | The in vitro effect of lipopolysaccharide on proliferation, inflammatory factors and antioxidant enzyme activity in bovine mammary epithelial cells显示文摘Lipopolysaccharide(LPS) was selected as a stimulus to investigate its effect on cell viability and oxidative stress in bovine mammary epithelial cells(BMEC) by detecting the cell relative growth rate(RGR),antioxidant indicators and inflammatory factors. This information was used to provide the theoretical basis for the establishment of a LPS-induced oxidative damage model. The experiment was divided into two parts. The first part used a two-factor experimental design to determine the appropriate incubation time of LPS by detecting the RGR. The third-passage BMEC were divided into 24 groups with six replicates in each group. The first factor was LPS concentration, which was 0(control), 0.1,1.0 and 10.0 μg/mL;the second factor was LPS incubation time(2,4, 6, 8,12 and 24 h). The optimum LPS incubation time was6 h according to the results of the first part of the experiment. The second part of the experiment was conducted using a single-factor experimental design, and the third-passage cells were divided into four groups with six replicates in each group. The cells were incubated with culture medium containing different concentrations of LPS(0 [control], 0.1, 1.0 and 10.0 μg/mL) for 6 h to select the appropriate concentration of LPS to measure the antioxidant indicators and inflammatory factors. The results showed the RGR was significantly reduced as the concentration of LPS and the incubation time increased;the interaction between concentration and incubation time was also significant. The cells treated with0.1 μg/mL of LPS for 6 h had no significant difference in the activities of glutathione peroxidase(GPx) and superoxide dismutase(SOD)(P > 0.05) compared with the cells in the control group. On the contrary,catalase(CAT) activity and malondialdehyde(MDA) content were markedly lower and higher, respectively, in the 0.1 μg/mL LPS-treated group for 6 h compared with the control group(P < 0.05). The activities of GPx, CAT and SOD in the BMEC treated with 1.0 or 10.0 μg/mL of LPS were significantly lower compared with the cells treated with 0.1 μg/mL of LPS and cells in the control group after 6 h of incubation; however, the opposite trend was detected in MDA content. There was no significant(P > 0.05)difference between the 10.0 and 1.0 μg/mL LPS-treated groups. Compared with the control group,interleukin-1, interleukin-6 and nitric oxide concentrations and the activity of inducible nitric oxide synthase in the 0.1 μg/mL LPS-treated group significantly increased(P < 0.0001), but the levels of tumour necrosis factor did not significantly change(P > 0.05). All of observed indicators were higher in the 1.0 and 10.0 μg/mL LPS-treated groups(P < 0.0001) compared with the other groups, but there was no significant(P> 0.05) difference between the 1.0 and 10.0 μg/mL LPS-treated groups. The results indicated that a concentration of 1.0 μg/mL of LPS and an incubation time of 6 h were the optimum conditions necessary to induce oxidative stress in the BMEC and establish a model for oxidative damage. | Huiyu Shi Yongmei Guo Yang Liu Binlin Shi Xiaoyu Guo Lu Jin Sumei Yan | 2016 | Animal Nutrition2016，，2： | 11 |
| 4 | The biogeochemical behavior of dissolved aluminum in the southern Yellow Sea:Influence of the spring phytoplankton bloom显示文摘Many recent studies have investigated the nutrient-type profiles of dissolved aluminum(Al) in the ocean.Significant scavenging of dissolved Al can occur during phytoplankton blooms,but the mechanism remains unclear.The distribution of dissolved Al in the southern Yellow Sea(SYS) was investigated in winter and spring 2009.Following measurements at grid stations during the spring sampling cruise,two drifting anchor surveys of more than 100 h were conducted to trace the variation of dissolved Al concentration during the spring phytoplankton bloom(SPB).The concentration of dissolved Al in the SYS decreased from 40 nmol/L in February to 30 nmol/L in March and 10-20 nmol/L in April,while the concentration of Chl a increased from < 2 μg/L in March to > 4 μg/L in April.The concentration of dissolved Al in the SYS decreased significantly with the development of the phytoplankton bloom,which indicated biological scavenging of dissolved Al from water column.The proportion of dissolved Al scavenged from water column by different phytoplankton species differed at the two drifting stations,with greater removal efficiency demonstrated by diatoms than dinoflagellates.Phytoplankton samples collected from the Chl a maximum layer were washed with trace metal clean reagent(oxalate-EDTA-citrate,abbreviate as oxalate solution,Tovar-Sanchez et al.,2003) to enable the surface-scavenged(extracellular) and intracellular Al pools associated with phytoplankton to be differentiated.Thirty-nine to ninetysix percent of the total Al was found to be existed in the interior pools,which indicated that biological absorption was the important way to scavenge dissolved Al during phytoplankton blooms in the SYS. | LI FaMing REN JingLing YAN Li LIU SuMei LIU ChengGang ZHOU Feng ZHANG Jing | 2013 | Chinese Science Bulletin2013，58，2： | 4 |
| 5 | Palm oil protectsα-linolenic acid from rumen biohydrogenation and muscle oxidation in cashmere goat kids显示文摘Background:In ruminants,dietary C18:3n-3 can be lost through biohydrogenation in the rumen;and C18:3n-3 that by-passes the rumen still can be lost through oxidation in muscle,theoretically reducing the deposition of C18:3n-3,the substrate for synthesis of poly-unsaturated fatty acids(n-3 LCPUFA)in muscle.In vitro studies have shown that rumen hydrogenation of C18:3n-3 is reduced by supplementation with palm oil(rich in cis-9 C18:1).In addition,in hepatocytes,studies with neonatal rats have shown that cis-9 C18:1 inhibits the oxidation of C18:3n-3.It therefore seems likely that palm oil could reduce both rumen biohydrogenation of C18:3n-3 and muscle oxidation of C18:3n-3.The present experiment tested whether the addition of palm oil to a linseed oil supplement for goat kids would prevent the losses of C18:3n-3 and thus improve the FA composition in two muscles,Longissimus dorsi and Biceps femoris.To investigate the processes involved,we studied the rumen bacterial communities and measured the mRNA expression of genes related to lipid metabolism in Longissimus dorsi.Sixty 4-month-old castrated male Albas white cashmere kids were randomly allocated among three dietary treatments.All three diets contained the same ingredients in the same proportions,but differed in their fat additives:palm oil(PMO),linseed oil(LSO)or mixed oil(MIX;2 parts linseed oil plus 1 part palm oil on a weight basis).Results:Compared with the LSO diet,the MIX diet decreased the relative abuandance of Pseudobutyrivibrio,a bacterial species that is positively related to the proportional loss rate of dietary C18:3n-3 and that has been reported to generate the ATP required for biohydrogenation(reflecting a decrease in the abundance of rumen bacteria that hydrogenate C18:3n-3 in MIX kids).In muscle,the MIX diet increased concentrations of C18:3n-3,C20:5n-3,C22:6n-3,and n-3 LCPUFA,and thus decreased the n-6/n-3 ratio;decreased the mRNA expression of CPT1β(a gene associated with fatty acid oxidation)and increased the mRNA expression of FADS1 and FADS2(genes associated with n-3 LCPUFA synthesis),compared with the LSO diet.Interestingly,compared to Longissimus dorsi,Biceps femoris had greater concentrations of PUFA,greater ratios of unsaturated fatty acids/saturated fatty acids(U/S),and poly-unsaturated fatty acids/saturated fatty acids(P/S),but a lesser concentration of saturated fatty acids(SFA).Conclusions:In cashmere goat kids,a combination of linseed and palm oils in the diet increases the muscle concentration of n-3 LCPUFA,apparently by decreasing the relative abundance of rumen bacteria that are positively related to the proportional loss rate of dietary C18:3n-3,by inhibiting mRNA expression of genes related to C18:3n-3 oxidation in muscle,and by up-regulating mRNA expression of genes related to n-3 LCPUFA synthesis in muscle,especially in Longissimus dorsi. | Xue Wang Graeme B.Martin Qi Wen Shulin Liu Yinhao Li Binlin Shi Xiaoyu Guo Yanli Zhao Yangdong Guo Sumei Yan | 2021 | Journal of Animal Science and Biotechnology2021，12，2： | 3 |
| 6 | Distribution of Dissolved Inorganic Arsenic and Its Seasonal Variations in the Coastal Area of the East China Sea显示文摘Water samples were collected in the coastal area of the Changjiang Estuary on four cruises from August 2002 to May 2003. The seasonal variations of dissolved inorganic arsenic (DIAs) distributions were analyzed. The results showed that the distributions of DIAs were mainly influenced by the terrestrial input and the intrusion of the Kuroshio Subsurface Water (KSSW). The concentration of the total dissolved inorganic arsenic (TDIAs) decreased consecutively from winter to summer, while it increased in autumn. The distributions of TDIAs showed some relationships with salinity and suspended particulate matter (SPM). The relationships between DIAs speciation (including arsenite [As(Ⅲ)] and arsenate [As(Ⅴ)]), biological activity and the availabilities of the phosphate were investigated in the study area for the cruise August 2002. The ratio of As (Ⅲ)/TDIAs increased with the decrease of phosphate concentrations. In the bottom water, the As(Ⅲ)/TDIAs ratio decreased with the increasing of N/P. The concentration of TDIAs decreased 28.7% approximately after the occurrence of harmful algal blooms (HAB) because of the uptake of arsenate by algae. Further study is needed about the arsenic source/sink relationships in their vertical or horizontal profiles and the uptake mechanism during the occurrence of harmful algal blooms. | CHENG Yan REN Jingling LI Dandan LIU Sumei ZHANG Jing | 2006 | Journal of Ocean University of China2006，5，3： | 3 |
| 7 | Effect of commercial slow-release urea product on in vitro rumen fermentation and ruminal microbial community using RUSITEC technique显示文摘Background:The objectives of this study were to determine the effect of commercial slow-release urea(SRU)on in vitro fermentation characteristics,nutrient digestibility,gas production,microbial protein synthesis and bacterial community using a rumen simulation technique(RUSITEC).The experiment was a completely randomized design with four treatments and four replications of each treatment.Treatments were:control diet(no SRU addition),control diet plus 0.28%SRU(U28),or plus 0.56%SRU(U56),and control diet that was modified substituting a part of soybean meal equivalent to 0.35%SRU(MU35;dry matter[DM]basis).The experiment consisted of 8 d of adaptation and 7 d of data and sample collection.Rumen inoculum was obtained from three ruminally fistulated Angus cows fed the same diet to the substrate incubated.Results:Digestibility of DM,organic matter(OM),crude protein(CP),fibre and starch was not affected,but daily production of gas(P<0.07)and methane(P<0.05)was quadratically increased with increasing SRU supplementation.The increase of SRU addition did not affect fermentation pH and total volatile fatty acid(VFA)production,whereas linearly(P<0.01)decreased proportion of propionate,and linearly(P<0.01)increased acetate to propionate ratio and ammonia nitrogen(N)concentration.The microbial N efficiency was also linearly(P<0.03)improved with increasing supplementation of SRU.In comparison with control diet,the dietary substitution of SRU for part of soybean meal increased(P<0.05)the digestibility of DM,OM and CP and decreased(P<0.02)the total gas production.The total VFA production and acetate to propionate ratio did not differ between control and MU35,whereas the proportion of butyrate was lower(P<0.05)and that of branched-chain VFA was greater(P<0.05)with MU35 than control diet.Total and liquid-associated microbial N production as well as ammonia N concentration were greater(P<0.03)with MU35 than control diet.Observed operational taxonomic units(OTUs),Shannon diversity index,and beta diversity of the microbial community did not differ among treatments.Taxonomic analysis revealed no effect of adding SRU on the relative abundance of bacteria at the phylum level,while at the genus level,the beneficial impact of SRU on relative abundance of Rikenellaceae and Prevotellaceae in feed particleassociated bacteria,and the abundance of Roseburia in liquid associate bacteria was greater(P<0.05)with MU35.Conclusions:Supplementation of a dairy cow diet with SRU showed potential of increase in ammonia N concentration and microbial protein production,and change fermentation pattern to more acetate production.Adding SRU in dairy cow diet also showed beneficial effect on improving digestibility of OM and fibre.The results suggest that SRU can partially substitute soybean meal in dairy cow diet to increase microbial protein production without impairing rumen fermentation. | Yongmei Guo Ling Xiao Long Jin Sumei Yan Dongyan Niu Wenzhu Yang | 2022 | Journal of Animal Science and Biotechnology2022，13，5： | 3 |
| 8 | Link adaptation based on adaptive modulation and coding for multiple-antenna OFDM system显示文摘 | TAN Penghui WU Yan SUN Sumei | | 0，，08： | 1 |
| 9 | Comparison the immunological activities of arabinoxylans from wheat bran with alkali and xylanase-aided extraction显示文摘 | Zhou Sumei Liu Xiuzhen Guo Yan | 2010 | Carbohydrate Polymers2010，81，： | 1 |
| 10 | A Low Complexity VBLAST OFDM Detection Algorithm for Wireless LAN Systems显示文摘 | Yan Wu Sun Sumei Lei Zhongding | 2004 | IEEE Commun Lett2004，8，6： | 1 |
| 11 | Comparison the immunological activities of arabinoxylans from wheatbran with alkali and xylanase-aided extraction显示文摘 | Zhou Sumei Liu Xiuzhen Guo Yan | 2010 | Carbohydrate Polymers2010，，81： | 1 |
| 12 | Effects of vitamin A on the milk performance, antioxidant functions and immune functions of dairy cows显示文摘 | Lu Jin Sumei Yan Binlin Shi Hongyun Bao Jian Gong Xiaoyu Guo Junliang Li | 2014 | Animal Feed Science and Technology2014，，： | 1 |
| 13 | 兴奋抑制间错配的多样性是大鼠听皮层局部功能异质性的基础显示文摘文章简介皮层神经元在功能特性上具有异质性(多样性)。这种异质性是处理不同类型的感觉信息的基础,但是对于局部神经元群内的功能异质性仍知之甚少。 | Can Tao Guangwei Zhang Chang Zhou Lijuan Wang Sumei Yan Huizhong Whit Tao Li I.Zhang 周艺 熊鹰 | 2018 | 科学新闻2018，0，4： | 0 |
| 14 | Properties of sandwich-structure based Mach-Zehnder interferometer cascaded with FBG response to dual-parameter sensing显示文摘An all-fiber temperature and curvature sensor based on Mach-Zehnder interferometer(MZI)was proposed.The MZI was a sandwich structure which is composed of ring-core fiber(RCF),no-core fiber(NCF)and single-mode fiber(SMF).The temperature and curvature can be demodulated by the wavelength shift and the intensity variation of the dips respectively in the transmission spectrum.The measurement results show that the sensitivity of curvature is−7.88 dBm/m^(−1)in the range from 3.0 m^(−1)to 4.2 m^(−1)and the sensitivity of temperature is 53.5 pm/°C in the range from 60°C to 200°C.In addition,the cascaded FBG in the proposed structure,also sensitive to temperature,was used to monitor the fluctuation of temperature.The compact structure,the real-time temperature and the high curvature sensitivity make the sensor have the potential in the field of construction health monitoring and mining safety production. | JIA Sumei YANG Kang LI Guoyu LI Yan WANG Zhihui | 2023 | Optoelectronics Letters2023，19，12： | 0 |
| 15 | A comparative study of the intestinal digestive characteristics of diferent feeds for Holstein cows显示文摘This experiment was conducted with lactating Chinese Holstein cows to study the nutritional value of local protein feed resources.A three-step method(TSP)and a modifed three-step method(MTSP)were used to measure the in vitro digestibility of rumen undegraded protein(RUP)for 11 feedstufs and correlation.Eleven experimental feeds were chosen and air-dried to investigate the efects of diferent growth periods and varieties on nutrition value and RUP digestibility.The small intestinal digestibility of RUP by TSP in concentrated feed was determined to be higher than that of roughage,approximately 65%.The highest concentrate(79%)was SBM(soybean mean),while the lowest was corn(65%).The proportions of DDGS(with soluble wine lees)and SFM(sunfower meal)were 70.9 and 74.9%,respectively.ASS(alfalfa mowed at the squaring stage)had the highest small intestinal digestibility of RUP(55%)among roughages,and WCS(whole-plant corn silage)had the lowest digestibility(40.5%).When the small intestinal digestibility of RUP was determined using the MTSP method,it exhibited similar results to the TSP method.Nevertheless,the values were generally higher,and there was a strong signifcant correlation between them(R2=0.967,P<0.01).The comparative study of these two methods help us have a better understanding of small intestine digestibility of diferent feeds,make a reasonable feed formula to efectively prevent diseases. | Shuai Han Fan Zhang Yanli Zhao Xiaoyu Guo Xiaowei Zhu Sumei Yan | 2022 | Animal Diseases2022，2，3： | 0 |
| 16 | From the water sources of the Tibetan Plateau to the ocean:State of nutrients in the Changjiang linked to land use changes and climate variability显示文摘Anthropogenic activity is an important driver of changes in the chemistry of nutrients(N,P,and Si)over watersheds at the sub-continental scale(e.g.,106km~2)and can markedly modify their seaward fluxes to the global ocean.In the present study,we reviewed the current status of nutrient chemistry in Changjiang(Yangtze River)based on data collected through 11 expeditions along a river course spanning 4,500 km and 15–20 major tributaries during 1997–2016 as well as monthly monitoring at the river mouth since 1980.The data were analyzed together with published results in the literature to synthesize the recent developments and current state of nutrients in the Changjiang.Previously published results from the Qinghai-Tibetan Plateau head waters were included to realize the systematics of nutrients for the whole drainage basin.Here,we showed that tributaries of the upper reaches of watersheds collectively determine the regime with high concentration and skewed species ratio of nutrients in the Changjiang mainstream,producing profound effects over a water course of 2,000–2,500 km further downstream and until the river mouth.Moreover,using data across the Three Gorges Reservoir(TGR)during 2003–2016,we evaluated the trapping and/or amplifying effects of the Three Gorges Dam(TGD)on nutrient chemistry.Tide-influenced river delta contributed an additional 20%dissolved inorganic phosphorus and 5–10%dissolved inorganic nitrogen and dissolved silicates to the seaward flux,dramatically affecting the stoichiometry of nutrients at the river mouth.Next,based on compiled data on supply and export,legacy nutrients were evaluated.Both nitrogen and phosphorus are in the accumulation phase over the watersheds,and the legacy nutrient fluxes are much higher than the annual riverine seaward fluxes.Finally,we demonstrated that the seaward fluxes of anthropogenic nutrients from the Changjiang exceed those from other top 10 largest rivers on this planet,which can be attributed to land use changes in the China over the last three to four decades. | Jing ZHANG Guosen ZHANG Yanan DU Anyu ZHANG Yan CHANG Yingchun ZHOU Zhuoyi ZHU Ying WU Zaifeng ZHANG Sumei LIU | 2022 | Science China Earth Sciences2022，65，11： | 0 |
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