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| 1 | Relationship between Fusobacterium nucleatum,inflammatory mediators and microRNAs in colorectal carcinogenesis显示文摘AIM To examine the effect of Fusobacterium nucleatum(F. nucleatum) on the microenvironment of colonic neoplasms and the expression of inflammatory mediators and microRNAs(miRNAs).METHODS Levels of F. nucleatum DNA, cytokine gene mRNA(TLR2, TLR4, NFKB1, TNF, IL1 B, IL6 and IL8), and potentially interacting miRNAs(miR-21-3p, miR-22-3p, mi R-28-5p, miR-34a-5p, miR-135b-5p) were measured by quantitative polymerase chain reaction(qPCR) TaqMan? assays in DNA and/or RNA extracted from the disease and adjacent normal fresh tissues of 27 colorectal adenoma(CRA) and 43 colorectal cancer(CRC) patients. KRAS mutations were detected by direct sequencing and microsatellite instability(MSI) status by multiplex PCR. Cytoscape v3.1.1 was used to construct the postulated miRNA:mRNA interaction network.RESULTS Overabundance of F. nucleatum in neoplastic tissue compared to matched normal tissue was detected in CRA(51.8%) and more markedly in CRC(72.1%). We observed significantly greater expression of TLR4, IL1 B, IL8, and miR-135 b in CRA lesions and TLR2, IL1 B, IL6, IL8, mi R-34 a and miR-135 b in CRC tumours compared to their respective normal tissues. Only two transcripts for miR-22 and miR-28 were exclusively downregulated in CRC tumour samples. The mRNA expression of IL1 B, IL6, IL8 and miR-22 was positively correlated with F. nucleatum quantification in CRC tumours. The mRNA expression of miR-135 b and TNF was inversely correlated. The miRNA:mRNA interaction network suggested that the upregulation of miR-34 a in CRC proceeds via a TLR2/TLR4-dependent response to F. nucleatum. Finally, KRAS mutations were more frequently observed in CRC samples infected with F. nucleatum and were associated with greater expression of miR-21 in CRA, while IL8 was upregulated in MSI-high CRC.CONCLUSION Our findings indicate that F. nucleatum is a risk factor for CRC by increasing the expression of inflammatory mediators through a possible mi RNA-mediated activation of TLR2/TLR4. | Marcela Alcantara Proenca Joice Matos Biselli Maysa Succi Fábio Eduardo Severino Gustavo Noriz Berardinelli Alaor Caetano Rui Manuel Reis David J Hughes Ana Elizabete Silva | 2018 | World Journal of Gastroenterology2018，24，47： | 15 |
| 2 | TLR2 and TLR4 polymorphisms influence m RNA and protein expression in colorectal cancer显示文摘AIM: To evaluate the effect of promoter region polymorphisms of toll-like receptor(TLR)2-196 to-174 del and TLR4-1607T/C(rs10759932) on m RNA and protein expression in tumor tissue and of TLR4+896A/G(rs4986790) on colorectal cancer(CRC) risk.METHODS: The TLR2-196 to-174 del polymorphism was investigated using allele-specific polymerase chain reaction(PCR) and the TLR4-1607T/C and TLR4+896A/G by PCR-restriction fragment length p o l y m o r p h i s m( R F L P). W e g e n o t y p e d 4 3 4 D N A samples from 194 CRC patients and 240 healthy individuals. The m RNA relative quantification(RQ) was performed in 40 tumor tissue samples by quantitative PCR Taq Man assay, using specific probes for TLR2 and TLR4 genes, and ACTB and GAPDH reference geneswere used as endogenous controls. Protein expression was analyzed by immunohistochemistry with specific primary antibodies.RESULTS: No association was found for TLR4-1607T/C and TLR4+896A/G by three statistical models(logadditive, dominant and recessive). However, based on dominant and log-additive models, the polymorphic variant TLR2-196 to-174 del was associated with increased CRC risk [dominant: odds ratio(OR) = 1.72, 95%CI: 1.03-2.89; P = 0.038 and log-additive: OR =1.59, 95%CI: 1.02-2.48; P = 0.039]. TLR2 m RNA expression was increased in tumor tissue(RQ = 2.36) when compared to adjacent normal tissue(RQ = 1; P < 0.0001), whereas the TLR4 m RNA showed a basal expression(RQ = 0.74 vs RQ = 1, P = 0.452). Immunohistochemistry analysis of TLR2 and TLR4 protein expression was concordant with the findings of m RNA expression. In addition, the TLR2-196 to-174 del variant carriers showed m RNA relative expression 2.19 times higher than wild-genotype carriers. The TLR2 protein expression was also higher for the TLR2-196 to-174 del variant carriers [117 ± 10 arbitrary unit(a.u.) vs 95 ± 4 a.u., P = 0.03]. However, for the TLR4-1607T/C polymorphism no significant difference was found for both m RNA(P = 0.56) and protein expression(P = 0.26).CONCLUSION: Our findings suggest that TLR2-196 to-174 del polymorphism increases TLR2 m RNA expression and is associated with higher CRC risk, indicating an important role in CRC genetic susceptibility. | Marcela Alcantara Proenca Juliana Garcia de Oliveira Aline Cristina Targa Cadamuro Maysa Succi Joao Gomes Netinho Eny Maria Goloni-Bertolo érika Cristina Pavarino Ana Elizabete Silva | 2015 | World Journal of Gastroenterology2015，21，25： | 9 |
| 3 | Lattice Boltzmann Analysis of Fluid-Structure Interaction with Moving Boundaries显示文摘This work is concerned with the modelling of the interaction of fluid flow with flexibly supported rigid bodies.The fluid flow is modelled by Lattice-Boltzmann Method,coupled to a set of ordinary differential equations describing the dynamics of the solid body in terms its elastic and damping properties.The time discretization of the body dynamics is performed via the Time Discontinuous Galerkin Method.Several numerical examples are presented and highlight the robustness and efficiency of the proposed methodology,by means of comparisons with previously published results.The examples show that the present fluid-structure method is able to capture vortexinduced oscillations of flexibly-supported rigid body. | Alessandro De Rosis Giacomo Falcucci Stefano Ubertini Francesco Ubertini Sauro Succi | 2013 | Communications in Computational Physics2013，13，3： | 1 |
| 4 | The prediction of helicopter rotor discrete frequency noise 显示文摘 | FARASSAT F SUCCI G P | 1983 | Vertica1983，7，4： | 1 |
| 5 | The Lattice Boltzmann Equation: Theory and Applications 显示文摘 | Benzi R Succi S Vergassola M | 1992 | Physics Report1992，222，： | 1 |
| 6 | Larg - scale cellular automata simulation of theimmune system response 显示文摘 | M Bemaschi S Succi | 2000 | Physical Review2000，2，： | 1 |
| 7 | The lattice Boltzmann equation: theory and applications显示文摘 | Benzi R Succi S Vergassola M | 1992 | Physics Report 2221992，，3： | 1 |
| 8 | The lattic Boltzmann equation on irregular lattices显示文摘 | NANNELLI F SUCCI S | 1996 | J Statist Phys1996，68，34： | 1 |
| 9 | Quantum Lattice Boltzmann Simulation of Expanding Bose-Einstein Condensates in Random Potentials 显示文摘 | Palpacelli S Succi S | 2008 | Physical Review E2008，77，06： | 1 |
| 10 | Genetic granular classifiers in modeling software quality显示文摘 | Witold Pedrycz Giancarlo Succi | | 0，，03： | 1 |
| 11 | Intermittency and structure functions in channel flow turbulence显示文摘 | Toschi F Amati G Succi S | 1999 | Physics Review Letter1999，82，25： | 1 |
| 12 | Harvesting the inferior epigastric artery through a transverse suprapubic incision显示文摘 | Da Costa Rocha B Succi JE Dauar RB | 2003 | Ann Thorac Surg2003，76，5： | 1 |
| 13 | Hydrodynamics in porous media: a finite volume lattice Boltzmann study 显示文摘 | ZARGHAMI A BISCAR1NI C SUCCI S | 2014 | Journal of Scientific Computing2014，59，1： | 1 |
| 14 | Quantitative assessment of extreme programming practices显示文摘 | SUCCI G STEFANOVIC M PEDRYCZ W | 2001 | Canadian Conference on Electrical and Computer Engineering2001，1，： | 1 |
| 15 | Lattice Boltzmann model for anisotropic liquid -solid phase transition显示文摘 | Miller W Succi S Mansutti D | 2001 | Physical review letters2001，86，16： | 1 |
| 16 | Determination of net energy of Italian ryegrass(Lolium multiflorum)silage and hay显示文摘 | SUCCI G CROVETTO G M TAMBURINI A el al | 1992 | Zoot Nutr Anim1992，18，： | 1 |
| 17 | Multiscale lattice Bohznmnn schemes with turbulence modeling 显示文摘 | FILIPPOVA o SUCCI S MAZZOCCO F | 2001 | Journal of Computational Physics2001，170，2： | 1 |
| 18 | Antibiotic susceptibility of Lactobacillus rhamnosus strains isolated from Pannigiano Reggiano cheese 显示文摘 | Coppola R Succi M Tremonte P | 2005 | Lait2005，85，3： | 1 |
| 19 | Effect of CPPU and pollination on fruiting performance, fruit quality and storage life of kiwifruit显示文摘 | Costa G Succi F Quadretti R | 1997 | ActaHort1997，444，： | 1 |
| 20 | A Lattice Boltzmann Model for Anisotropic Crystal Growth from Melt显示文摘 | W. Miller S. Succi | 2002 | Journal of Statistical Physics (-)2002，，1： | 1 |
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