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| 1 | Evaluation of clinical relevance of examining K-ras, p16 and p53 mutations along with allelic losses at 9p and 18q in EUS-guided fine needle aspiration samples of patients with chronic pancreatitis and pancreatic cancer显示文摘AIM: To establish an optimum combination of molecular markers resulting in best overall diagnostic sensitivity and specificity for evaluation of suspicious pancreatic mass. METHODS: Endoscopic ultrasound (EUS)-guided fine needle aspiration cytology (FNA) was performed on 101 consecutive patients (63 males, 38 females, 60 ± 12 years; 81 with subsequently diagnosed pancreatic cancer, 20 with chronic pancreatitis) with focal pancreatic mass. Samples were evaluated on-site by an experienced cytopathologist. DNA was extracted from Giemsa stained cells selected by laser microdissection and the presence of K-ras, p53 and p16 somatic mutations was tested by cycling-gradient capillary electrophoresis (CGCE) and single-strand conformation polymorphism (SSCP) techniques. In addition, allelic losses of tumor suppressor genes p16 (INK4, CDKN2A) and DPC4 (MADH4, SMAD4) were detected by monitoring the loss of heterozygosity (LOH) at 9p and 18q, respectively. RESULTS: Sensitivity and specificity of EUS-guided FNA were 75% and 85%, positive and negative predictivevalue reached 100%. The remaining 26% samples were assigned as inconclusive. Testing of molecular markers revealed sensitivity and specificity of 70% and 100% for K-ras mutations (P < 0.001), 24% and 90% for p53 mutations (NS), 13% and 100% for p16 mutations (NS), 85% and 64% for allelic losses at 9p (P < 0.001) and 78% and 57% for allelic losses at 18q (P < 0.05). When tests for different molecular markers were combined, the best results were obtained with K-ras + LOH at 9p (92% and 64%, P < 0.001), K-ras + LOH at 18q (92% and 57%, P < 0.001), and K-ras + LOH 9q + LOH 18q (96% and 43%, P < 0.001). When the molecular markers were used as complements to FNA cytology to evaluate inconclusive samples only, the overall sensitivity of cancer detection was 100% in all patients enrolled in the study. CONCLUSION: EUS-guided FNA cytology combined with screening of K-ras mutations and allelic losses of tumor suppressors p16 and DPC4 represents a very sensitive approach in screening for pancreatic malignancy. Molecular markers may find its use particularly in cases where FNA cytology has been inconclusive. | C Salek L Benesova M Zavoral V Nosek L Kasperova M Ryska R Strnad E Traboulsi M Minarik | 2007 | World Journal of Gastroenterology2007，13，27： | 18 |
| 2 | Ethylene interacts with acts with abscisic acid to regulate endosperm rupture during germination: a comparative approach using Lepidiumsativur and Arabidopsis thaliana 显示文摘 | Linkies A Muller K Morris K Tureckova V Wenk M Cadman CS Corbineau F Strnad M Lynn J R Finch-Savage W E Leubner-Metzger G | 2009 | Plant Cell2009，21，12： | 1 |
| 3 | Regulation of plant growth by cytokinin显示文摘 | Werner T Motyka V Strnad M | 2001 | Proceedings of the National A- cademy of Science of USA2001，98，10： | 1 |
| 4 | Californium-252 (zsz Cf) versus conventional gamma radiation in the braChytherapy of advanced cervical carcinoma long-term treatment results of a randomized study 显示文摘 | Tacev T Ptackova B Strnad V | 2003 | Strahlenther Onkol2003，179，6： | 1 |
| 5 | Nucleotide sequence,organization and characterization of the (halo)aromatic acid catabolic plasmid pAS1 from Achromobacter xylosoxidans AS显示文摘 | JENCOVA V STRNAD H CHODORA Z | 2008 | Research in Microbiology2008，159，2： | 1 |
| 6 | Predictive value of intraosseous venography before percutaneous vertebroplasty显示文摘 | KEVIN J HEATWOLE E V STRNAD B T | 2002 | J Vasc Interv Radiol2002，13，2： | 1 |
| 7 | Californium-252 (252 Cf) versus conventional gamma radiation in the brachytherapy of advanced cervical carcinoma long-term treament results of a randomized study显示文摘 | Taeev T PtaOekov6 B Strnad V | 2003 | Strahlenther Onkol2003，179，6： | 1 |
| 8 | Radiochemotherapy in the liver metastases of cystosarcoma phyllodes 显示文摘 | Riepl M Strnad V | 1994 | Strahlenther Onkol1994，170，11： | 1 |
| 9 | Regulation of plant growth by cytokinin显示文摘 | WERNER T MOTYKA V STRNAD M etal | 2001 | Proceedings of the Nation al Academy of Sciences2001，98，10： | 1 |
| 10 | Regulation of plant growth by cytokinin显示文摘 | WERNER T MOTYKA V STRNAD M | 2001 | Proc Natl Acad Sci USA2001，98，10： | 1 |
| 11 | Accelerated partial breast irradiation: 5-year results of the German-Austrian multicenter phase 1I trial using interstitial multicatheter brachytherapy alone af- ter breast-conserving surgery显示文摘 | Strnad V Hildebrandt G Ptter R | 2011 | Int j Radiat Oncol Biol Phys2011，80，1： | 1 |
| 12 | Pre- dictive value of intraosseous venography before percutaneous ver- tebroplasty显示文摘 | MCGRAW J K HEATWOLE E V STRNAD B T | 2002 | J VascInterv Radiol2002，13，： | 1 |
| 13 | The role of pulsed-dose-rate brachytherapy in previously irradiated head-and-neck cancer显示文摘 | STRNAD V GEIGER M LOTTER M | 2003 | Brachytherapy2003，2，： | 1 |
| 14 | Chlorocatechol catabolic enzymes from Achromobacter xylosoxidans A8显示文摘 | Jencova V Strnad H Chodora Z | 2004 | Int Biodeterior Biodegradation2004，54，23： | 1 |
| 15 | Accelerated partial breast irradiation:5-year results of the German-Austrianmuhicenter phase II trial using interstitial multicatheter braehytherapy aloneafter breast-conserving surgery显示文摘 | Strnad V Hildebrandt G Potter R | 2011 | Int J Radiat Oncol Biol Phys2011，80，： | 1 |
| 16 | Californium-252 (252Cf)versus conventional gamma radiation in the brachytherapy of advanced cervical carcinoma long-term treatment results of a randomized study显示文摘 | Tacev T Ptackova B Strnad V | 2003 | Strahlenther Onkol2003，179，6： | 1 |
| 17 | Ethylene interacts with acts with abscisic acid to regulate endosperm rupture during germination : a comparative approach using Lepidiumsativur and Arabidopsis thaliana显示文摘 | Linkies A Muller K Morris K Tureckova V Wenk M Cadman C S Corbineau F Strnad M Lynn J R Finch-Savage W E Leubner- Metzger G | 2009 | Plant Cell2009，21，12： | 1 |
| 18 | Interstitial brachytherapy alone after breast conserving surgery:Interim results of a German-Austrian multicenter phase Ⅱ trial显示文摘 | Strnad V Ott O Hildebrandt G | 2004 | Brachy2004，3，3： | 1 |
| 19 | Brachytherapy for partial breast irradiation:the european experience显示文摘 | Polgar C Strnad V Major T | 2005 | Semin Radiat Oncol2005，15，2： | 1 |
| 20 | Chlorocatechol catabolic enzymes from Achromobacter xylosoxidans A8显示文摘 | JENCOVA V STRNAD H CHODORA Z | 2004 | International Biodeterioration & Biodegradation2004，54，23： | 1 |
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