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| 1 | Evaluation of clinical relevance of examining K-ras, p16 and p53 mutations along with allelic losses at 9p and 18q in EUS-guided fine needle aspiration samples of patients with chronic pancreatitis and pancreatic cancer显示文摘AIM: To establish an optimum combination of molecular markers resulting in best overall diagnostic sensitivity and specificity for evaluation of suspicious pancreatic mass. METHODS: Endoscopic ultrasound (EUS)-guided fine needle aspiration cytology (FNA) was performed on 101 consecutive patients (63 males, 38 females, 60 ± 12 years; 81 with subsequently diagnosed pancreatic cancer, 20 with chronic pancreatitis) with focal pancreatic mass. Samples were evaluated on-site by an experienced cytopathologist. DNA was extracted from Giemsa stained cells selected by laser microdissection and the presence of K-ras, p53 and p16 somatic mutations was tested by cycling-gradient capillary electrophoresis (CGCE) and single-strand conformation polymorphism (SSCP) techniques. In addition, allelic losses of tumor suppressor genes p16 (INK4, CDKN2A) and DPC4 (MADH4, SMAD4) were detected by monitoring the loss of heterozygosity (LOH) at 9p and 18q, respectively. RESULTS: Sensitivity and specificity of EUS-guided FNA were 75% and 85%, positive and negative predictivevalue reached 100%. The remaining 26% samples were assigned as inconclusive. Testing of molecular markers revealed sensitivity and specificity of 70% and 100% for K-ras mutations (P < 0.001), 24% and 90% for p53 mutations (NS), 13% and 100% for p16 mutations (NS), 85% and 64% for allelic losses at 9p (P < 0.001) and 78% and 57% for allelic losses at 18q (P < 0.05). When tests for different molecular markers were combined, the best results were obtained with K-ras + LOH at 9p (92% and 64%, P < 0.001), K-ras + LOH at 18q (92% and 57%, P < 0.001), and K-ras + LOH 9q + LOH 18q (96% and 43%, P < 0.001). When the molecular markers were used as complements to FNA cytology to evaluate inconclusive samples only, the overall sensitivity of cancer detection was 100% in all patients enrolled in the study. CONCLUSION: EUS-guided FNA cytology combined with screening of K-ras mutations and allelic losses of tumor suppressors p16 and DPC4 represents a very sensitive approach in screening for pancreatic malignancy. Molecular markers may find its use particularly in cases where FNA cytology has been inconclusive. | C Salek L Benesova M Zavoral V Nosek L Kasperova M Ryska R Strnad E Traboulsi M Minarik | 2007 | World Journal of Gastroenterology2007，13，27： | 18 |
| 2 | The importance of homnnone receptors in benign breast disease显示文摘 | Strnad P Zavadil M DanesJ | 1998 | Ceska Gynekol1998，63，1： | 1 |
| 3 | Ethylene interacts with acts with abscisic acid to regulate endosperm rupture during germination: a comparative approach using Lepidiumsativur and Arabidopsis thaliana 显示文摘 | Linkies A Muller K Morris K Tureckova V Wenk M Cadman CS Corbineau F Strnad M Lynn J R Finch-Savage W E Leubner-Metzger G | 2009 | Plant Cell2009，21，12： | 1 |
| 4 | Arabidopsis cyto- kinin receptor mutants reveal functions in shoot growth, leaf senescence, seed size, germination, root develop- ment, and cytokinin metabolism 显示文摘 | Riefler M Novak O Strnad M | 2006 | The Plant Cell2006，18，1： | 1 |
| 5 | Keratin 8 overexpression promotes mouse Mallory body formation显示文摘 | NAKAMICHI I TOIVOLA D M STRNAD P | 2005 | J Cell Biol2005，171，6： | 1 |
| 6 | Keratin overexpression levels correlate with the extent of spontaneous pancreatic injury显示文摘 | TOIVOLA D M NAKAMICHI I STRNAD P | 2008 | Am J Pathol2008，172，4： | 1 |
| 7 | Correction: Combination of lung ultrasound (a comet-tail sign)and N-terminal pro-brain natri- uretic peptide in differentiating acute heart failure from chronic ob- structive pulmonary disease and asthma as cause of acute dyspnea in prehospital emergency setting 显示文摘 | Prosen G Klemen P Strnad M | 2011 | Crit Care2011，15，6： | 1 |
| 8 | Regulation of plant growth by cytokinin显示文摘 | Werner T Motyka V Strnad M | 2001 | Proceedings of the National A- cademy of Science of USA2001，98，10： | 1 |
| 9 | Presentation,management and outcome of 32 patients with pregnancy-associated breast cancer: a matched controlled study显示文摘 | Halaska M J Pentheroudakis G Strnad P | 2009 | Breast J2009，15，5： | 1 |
| 10 | Correctimt Com- bination of hmg ullrasound (a comet-tail sign) and N- terminal pro-brain natriuretic peptide in differentiating acute heart failure from chronic obstructive pulmonary disease and asthma as cause of acute dyspnea in prehospital emergency setting 显示文摘 | Prosen G Klemen P Strnad M | 2011 | Crit Care2011，15，6： | 1 |
| 11 | Arabidopsis cytokinin receptormutants reveal functions in shoot growth,leaf senescence,seed size,ger-mination,root development,and cytokinin metabolism显示文摘 | RIEFLER M NOVAK O STRNAD M | 2006 | Plant Cell2006，18，： | 1 |
| 12 | Combination of lung ultrasound (a comet-tail sign) and N-terminal pro-brain natriuretic peptide in differentiating acute heart failure fi:om chronic obstructive pulmonary disease and asthma as cause of acute dyspnea in prehospital emergen- cy setting显示文摘 | Prosen G Klemen P Strnad M | 2011 | Crit Care2011，15，2： | 1 |
| 13 | Role of insulin- like growth factor-1 in colon cancerogenesis : a case-control study显示文摘 | Tripkovic I Tripkovic A Strnad M | 2007 | Arch Med Res2007，38，5： | 1 |
| 14 | Lymphatic Mapping in Endometrial Cancer Comparison of Hysteroscopic and Subserosal Injection and the Distribution of Sentinel Lymph Nodes显示文摘 | Robova H Charvat M Strnad P | 2009 | Int J Gynecol Cancer2009，19，3： | 1 |
| 15 | Combination of lung ultrasound(a comet-tail sign)and N-terminal pro-brain natriuretic peptide in differentiating acute heart failure from chronic obstructive pulmonary disease and asthma as cause of acute dyspnea in prehospital emergency setting显示文摘 | Prosen G Klemen P Strnad M | 2011 | Crit Care2011，15，2： | 1 |
| 16 | Salt and cancer 显示文摘 | Strnad M | 2010 | Acta Med Croatica2010，64，2： | 1 |
| 17 | Radiochemotherapy in the liver metastases of cystosarcoma phyllodes 显示文摘 | Riepl M Strnad V | 1994 | Strahlenther Onkol1994，170，11： | 1 |
| 18 | The importance of hormone receptors in benign breast diseases显示文摘 | Strnad P Zavadil M Danes J | 1998 | Ceska Gyneko1998，63，1： | 1 |
| 19 | Sources of soil and plant con-tamination in an urban environment and possible assessmentmethods 显示文摘 | Sanka M Strnad M Vondra J | 1995 | International Journal of Environmental Analytical Chemistry1995，59，： | 1 |
| 20 | Regulation of plant growth by cytokinin显示文摘 | WERNER T MOTYKA V STRNAD M etal | 2001 | Proceedings of the Nation al Academy of Sciences2001，98，10： | 1 |
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