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| 1 | Cytotoxic CD8+ T cells and tissue resident memory cells in colorectal cancer based on microsatellite instability and BRAF status显示文摘BACKGROUND Recent studies in non-colorectal malignancy have associated T resident memory(T_(RM)) cells with improved patient survival. It is unknown if T_(RM) plays a role in colorectal cancer(CRC).AIM To examine the potential role of T_(RM) cells in providing immunogenicity in CRC stratified by microsatellite instability(MSI) and BRAF status.METHODS Patients with known MSI and BRAF mutation status were eligible for inclusion in this study. CRC tumour sections stained with haematoxylin and eosin were microscopically reviewed and the images scanned prior to assessment for location of invading edge and core of tumour. Sequential sections were prepared for quantitative multiplex immunohistochemistry(IHC) staining. Opal Multiplex IHC staining was performed with appropriate positive and negative controls and imaged using a standard fluorescent microscope fitted with a spectral scanning camera(Mantra) in conjunction with Mantra snap software. Images were unmixed and annotated in in Form 2.2.0. Statistical analysis was performed using Graphpad Prism Version 7 and Stata Version 15.RESULTS Seventy-two patients with known MSI and BRAF status were included in the study. All patients were assessed for MSI by IHC and high resolution capillary electrophoresis testing and 44 of these patients successfully underwent quantitative multiplex IHC staining. Overall, there was a statistically significant increase in CD8+ T_(RM) cells in the MSI(BRAF mutant and wild type) group over the microsatellite stable(MSS) group. There was a statistically significant difference in CD8+ T_(RM) between high level MSI(MSI-H):BRAF mutant [22.57, 95% confidence interval(CI): 14.31-30.84] vs MSS [8.031(95%CI: 4.698-11.36)], P = 0.0076 and MSI-H:BRAF wild type [16.18(95%CI: 10.44-21.93)] vs MSS [8.031(95%CI: 4.698-11.36)], P = 0.0279. There was no statistically significant difference in CD8 T cells(both CD8+CD103-and CD8+CD103+T_(RM)) between MSI-H: BRAF mutant and wild type CRC.CONCLUSION This study has shown that CD8+ T_(RM) are found in greater abundance in MSI-H CRC, both BRAF mutant and MSI-H:BRAF wild type, when compared with their MSS counterpart. CD8+ T_(RM) may play a role in the immunogenicity in MSI-H CRC(BRAF mutant and BRAF wild type). Further studies should focus on the potential immunogenic qualities of T_(RM) cells and investigate potential immunotherapeutic approaches to improve treatment and survival associated with CRC. | James Wei Tatt Toh Angela L Ferguson Kevin J Spring Hema Mahajan Umaimainthan Palendira | 2021 | World Journal of Clinical Oncology2021，12，4： | 2 |
| 2 | Colorectal carcinogenesis:Road maps to cancer显示文摘 | Daniel L Worthley Vicki L Whitehall Kevin J Spring Barbara A Leggett | 2007 | World Journal of Gastroenterology2007，，28： | 2 |
| 3 | The network weather service:A distributed resource performance forecasting service for metacomputing显示文摘 | Wolski R Spring N Hayes J | 1999 | Journal of Future Generation Computing Systems1999，15，5： | 2 |
| 4 | Changes in the expression and localization of hepatocellular transporters and radixin in primary biliary cirrhosis显示文摘 | Hideyuki Kojima Anne T Nies J?rg K?nig Wolfgang Hagmann Herbert Spring Masahito Uemura Hiroshi Fukui Dietrich Keppler | 2003 | Journal of Hepatology2003，，5： | 1 |
| 5 | The network weather service: a distributed resource performance forecasting service for meta computing显示文摘 | WOLSKI R SPRING N HAYES J | 1999 | Future Generation Computer Systems1999，15，10： | 1 |
| 6 | Does social media im- prove communication?Evaluating the NIOSH science blog显示文摘 | V Sublet C Spring J Howard National Institute of Oc- cupational Safety and Health | 2011 | Am J Ind Med2011，54，5： | 1 |
| 7 | Growth performance, carcass characteristics and meat quality of group-penned surgically castrated, immunocastrated (Improvac) and entire male pigs and individually penned entire male pigs显示文摘 | Pauly C Spring P OfDoherty J V | 2009 | Animal2009，37，： | 1 |
| 8 | Does social media improve communication? Evaluating the NIOSH sci- ence blog显示文摘 | V Sublet C Spring J Howard National Institute of Occupational Safety and Health | 2011 | AmJ lnd Med2011，54，5： | 1 |
| 9 | Fabrication and Characterization of ZrB:-Based Ceramic Using Syrthesized ZrB2-LaB6 Powder显示文摘 | Spring A Guo W M Zhang G J | 2008 | Journal of the American Ceramic Society2008，91，8： | 1 |
| 10 | Analysis of unknown primary carcinomas metastatic to the neck: diagnosis, treatment, and outcornes显示文摘 | Doty J M Gossman D Kudrimoti M Valentino J Arnold S Spring P M | 2006 | J Ky Med Assoc2006，104，： | 1 |
| 11 | Flow rates East Pacific Rise(21°N) implications for the heat budget and the formation of massive sulfide deposits显示文摘 | Converse D R Holland H D in the axial hot springs of the Edmond J M | 1984 | Earth and Planet Science Letters1984，69，： | 1 |
| 12 | Performances, meat qual- ity and boar taint of castrates and entire male pigs fed a standard and a raw potato starch-enriched diet 显示文摘 | Pauly C Spring P O'Doherty J V | 2008 | Animal2008，2，11： | 1 |
| 13 | The Network Weather Service:A Distributed Resource Performance Forecasting Service for Metacomputing显示文摘 | Wolski R Spring N Hayes J | 1998 | Journal of Future Generation Computing Systems1998，15，56： | 1 |
| 14 | Quantum proto- cols for anonymous voting and surveying 显示文摘 | VACCARO J A SPRING J CHEFLES A | 2007 | Physical Re- view A2007，75，01： | 1 |
| 15 | 18F flurodeoxyglu-cose positron emission tomography in the localization of ACTtt-secreting neuroendocrine tumours 显示文摘 | Kumar J Spring M Carroll PV | 2006 | Clin Endo- erinol2006，64，4： | 1 |
| 16 | Growth performance, carcass characteristics and meat quality of group-penned surgically castrated, immunocastrated (Improvac ) and entire male pigs and individually penned entire male pigs 显示文摘 | Pauly C Spring P O'Doherty J | 2009 | Animal2009，3，7： | 1 |
| 17 | Imaging and photodynamic therapy: mechanisms, monitoring, and optimization显示文摘 | Celli J P Spring B Q Rizvi I | 2010 | Chem Rev2010，110，5： | 1 |
| 18 | Terahertz quantum-well photodetector显示文摘 | Liu H C Song C Y Spring Thorpe A J | | 0，，20： | 1 |
| 19 | Atrial premature beats pre- dict atrial fibrillation in cryptogenic stroke: results from the EM- BRACE trial显示文摘 | Gladstone D J Dorian P Spring M | 2015 | Stroke2015，46，4： | 1 |
| 20 | Coumarin-derived transformable fluorescent sensor for Zn2+显示文摘 | Xu Z C Liu X Pan J Spring D R | 2012 | Chemical Communications2012，48，39： | 1 |
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