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| 1 | Base-Editing-Mediated Artificial Evolution of OsALS1 In Planta to Develop Novel Herbicide-Tolerant Rice Germplasms显示文摘Recently developed CRISPR-mediated base editors,which enable the generation of num erous nucleotide changes in target genomic regions,have been widely adopted for gene correction and generation of crop germ plasms containing im portant gain-of-function genetic variations.How ever,to engineer target genes with unknown functional SNPs remains challenging.To address this issue,we present here abase-e diting-mediated gene evolution(BEMGE)m ethod,employing both Cas9n-based cytosine and adenine base editors as well as a single-guide RNA(sgRNA)library tiling the full-length coding region,for developing novel rice germ plasm swith mutations in any endogenous gene.To this end,OsALS1 was artificially evolved in rice cells using BEMGE through both Agrobacterium-mediated and particle-bom bardment-mediated transform ation.Four different types of amino acid substitutions in the evolved OsALS1,derived from two sites that have never been targeted by natural or human selection during rice dom estication,were identified,conferring varying levels of tolerance to the herbicide bispyribac-sodium.Furtherm ore,the P171F substitution identified in a strong OsALS1 allele was quickly introduced into the commercial rice cultivar Nangeng 46 through precise base editing w ith the corresponding base editor and sgRNA.Collectively,these data indicate great potential of BEMGE in creating important genetic variants of target genes for crop improvement. | Yongjie Kuang Shaofang Li Bin Ren Fang Yan Carl Spetz Xiangju Li Xueping Zhou Huanbin Zhou | 2020 | Molecular Plant2020，13，4： | 37 |
| 2 | LJItrasensitive H2S gas sensors based on p-type WS2 hybrid materials显示文摘 | Georgies Alene Asrest Jose J. Baldovi Aron Dombovari Topias Jarvinen Gabriela Simone Lorite Melinda Mohl Andrey Shchukarev Alejandro Perez Paz Lede Xian Jyri-Pekka Mikkola Anita Lloyd Spetz Heli Jantunen Angel Rubio Krisztian Kordas | 2018 | Nano Research2018，11，8： | 3 |
| 3 | Baccalaureate Education in Nursing and Patient Outcomes显示文摘 | Mary A. Blegen Colleen J. Goode Shin Hye Park Thomas Vaughn Joanne Spetz | 2013 | JONA: The Journal of Nursing Administration2013，，： | 2 |
| 4 | Evaluation of gas mixtures with high temperature gas sensors based on silicon carbide 显示文摘 | Arbab A Spetz A Lundstrom I | 1994 | Sensors and Actuators B1994，，1819： | 1 |
| 5 | Hospital information technology systems' impact on nurses and nursing care 显示文摘 | Waneka R Spetz J | 2010 | The Journal of Nurs- ing Administration2010，40，： | 1 |
| 6 | Nurse satisfaction and the implementation of minimum nurse staffing regulations显示文摘 | Spetz J | | 0，，01： | 1 |
| 7 | Current status of silicon carbide based high-temperature gas sensors 显示文摘 | Spetz A L Tobias P Baranzahi A | 1999 | IEEE Trans Elec Dev1999，46，3： | 1 |
| 8 | More intramedullary nails and arthroplasties for treatment of hip fractures in Sweden显示文摘 | Cecilia Rogmark Curt-Lennart Spetz G?ran Garellick | 2010 | Acta Orthopaedica2010，，5： | 1 |
| 9 | Gas sensors for high temperature operation based on metal oxide silicon carbide (MOSiC) devices 显示文摘 | Arbab A Spetz A L Lundstrom I | 1993 | Sensors and Actuators B1993，15，13： | 1 |
| 10 | Nurse staffing and hospital ownership in California 显示文摘 | Seago JA Spetz J Mitchell S | 2004 | J Nurs Adm2004，34，5： | 1 |
| 11 | Potyviral 6K2 protein long-distance move- ment and symptom-induction functions ale independent and host- specific 显示文摘 | Spetz C Valkonen J P T | 2004 | Molecular Plant-Microbe Interactions2004，17，5： | 1 |
| 12 | Response of metal-oxide-silicon carbide sensors to simulated and real exhaust gases显示文摘 | Spetz A L Glavmo M | 1997 | Sensors and Actuators B1997，43，： | 1 |
| 13 | The effects of managed care and prospective payment on the demand for hospital nurses: Evidence from California 显示文摘 | Spetz J | 1999 | Health Service Research1999，34，5： | 1 |
| 14 | Current status of silicon carbide based high-temperature gas sensors显示文摘 | Tobias P Baranzahi A | 1999 | IEEE Transaction on Electron Devices1999，46，3： | 1 |
| 15 | Nanoparticles as the active element of high-temperature metal-insulator-silicon carbide gas sensors显示文摘 | BOHME O SPETZ A L LUNDSTROM I | 2001 | Advanced Materials2001，13，8： | 1 |
| 16 | High temperature catalytic metal field effect transistors for industrial application显示文摘 | TOBIAS P UNEUS L | 2000 | Sensors and Actuators B2000，70，13： | 1 |
| 17 | California's minimum nurse-to-patient ratios:the first few months 显示文摘 | Spetz J | 2004 | J Nurs Adm2004，34，12： | 1 |
| 18 | A specialist nurse as a resource for family members to patients with brain tumors : An action research study显示文摘 | Spetz A Henriksson R Salander P | 2008 | Cancer Nursing2008，31，4： | 1 |
| 19 | Soil organic matter quality as a link between microbial commtmity structure and vegetation composition along a successional gradient in a boreal forest显示文摘 | Merilǎ P Malmivaara-L(a)ms(a) M Spetz P Stark S Vierikko K Derome J Fritze H | | 0，，02： | 1 |
| 20 | Current status of silicon carbide based high-temperature gas sensors显示文摘 | TOBIAS P BARANZAHI A | 1999 | IEEEE Trans Electron Device1999，46，3： | 1 |
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