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| 1 | Work, home, and mar-ket: the social transformation of housing space in Guangzhou, China显示文摘 | Li Siming Hou Quan Chen Susu | 2010 | Urban Geography2010,31,4: | 1 |
| 2 | Transport infrastructure development and changing spatial accessibility in the Greater Pearl River Delta, China, 1990-2020显示文摘 | HOU Quan LI Siming | 2011 | Journal of Transport Geography2011,19,: | 1 |
| 3 | National incidence of joint dislocation in China:a retrospective survey of 512,187 individuals显示文摘Background:Joint dislocations significantly impact public health.However,a comprehensive study on the incidence,distribution,and risk factors for joint dislocations in China is lacking.We conducted the China National Joint Dislocation Study,which is a part of the China National Fracture Study conducted to obtain the national incidence and risk factors for traumatic fractures,and to investigate the incidence and risk factors for joint dislocations.Methods:For this national retrospective epidemiological study,512,187 participants were recruited using stratified random sampling and probability-proportional-to-size method from January 19 to May 16,2015.Participants who sustained joint dislocations of the trunk,arms,or legs(skull,sternum,and ribs being excluded)in 2014 were personally interviewed to obtain data on age,educational background,ethnic origin,occupation,geographic region,and urbanization degree.The joint-dislocation incidence was calculated based on age,sex,body site,and demographic factors.The risk factors for different groups were examined using multiple logistic regression.Results:One hundred and nineteen participants sustained 121 joint dislocations in 2014.The population-weighted incidence rate of joint dislocations of the trunk,arms,or legs was 0.22(95%confidence interval[CI]:0.16,0.27)per 1000 population in 2014(men,0.27[0.20,0.34];women,0.16[0.10,0.23]).For all ages,previous dislocation history(male:OR 42.33,95%confidence interval[CI]:12.03-148.90;female:OR 54.43,95%CI:17.37-170.50)and alcohol consumption(male:OR 3.50,95%CI:1.49-8.22;female:OR 2.65,95%CI:1.08-6.50)were risk factors for joint dislocation.Sleeping less than 7 h/day was a risk factor for men.Compared with children,women aged≥15 years(female 15-64 years:OR 0.16,95%CI:0.04-0.61;female≥65 years:OR 0.06,95%CI:0.01-0.41)were less likely to sustain joint dislocations.Women with more than three children were at higher dislocation risk than women without children(OR 6.92,95%CI:1.18-40.78).Conclusions:The up-to-date data on joint dislocation incidence,distribution,and risk factors can be used as a reference for national healthcare,prevention,and management in China.Specific strategies for decreasing alcohol consumption and encouraging adequate sleeping hours should be developed to prevent or reduce dislocation incidents.Trial Registration:Chinese Clinical Trial Registry,ChiCTR-EPR-15005878. | Hongzhi Lv Wei Chen Zhiyong Hou Siming Jia Yanbin Zhu Bo Liu Xiao Chen Guang Yang Lei Liu Tao Zhang Haili Wang Bing Yin Song Liu Jialiang Guo Xiaolin Zhang Yichong Li Yingze Zhang | 2022 | Chinese Medical Journal2022,,14: | 0 |
| 4 | Construction of Lactuca sativa Plastid Transformation Vector and High-level Expression of gfp Gene in Escherichia coli显示文摘Using genomic DNA of bolting-tolerant lettuce as a template,flanking fragments of lettuce plastid rpo A gene were amplified and cloned by PCR. Targeting the sites of these two fragments,homologous recombinant fragments of exogenous gene were integrated to construct lettuce plastid expression vector p Brpo AGFP,which harbored the expression cassette Prrn-gfp-aad A-Tpsb A. The results showed that the amplified flanking fragments were 1.2 and 1.1 kb in size. After sequencing,restriction digestion,ligation and transformation,lettuce plastid expression vector containing expression cassette Prrn-gfp-aad A-Tpsb A was constructed and confirmed by SDS-PAGE electrophoresis. The results of SDS-PAGE electrophoresis indicated that gfp gene was efficiently expressed under the regulation of plasmid specific promoter Prrn and terminator Tpsb A. GFP accounted for 45. 6% of total soluble proteins; inclusion bodies accounted for 47.5 % of bacterial proteins,which reached relatively high expression levels. The construction of lettuce plastid expression vector p Brpo A-GFP laid a solid foundation for establishment of subsequent lettuce plastid transformation system and genetic improvement of lettuce using various functional genes. | Siming HOU Liying ZHOU Lulu BU Chunlei YANG Ting GAO Tian TIAN Zheng'an YANG | 2016 | Agricultural Biotechnology2016,5,4: | 0 |
| 5 | Temperature‑Gated Light‑Guiding Hydrogel Fiber for Thermoregulation During Optogenetic Neuromodulation显示文摘With the rise of optogenetic manipulation of neurons,the effects of optogenetic heating on temperature-sensitive physi-ological processes,and the damage to surrounding tissues have been neglected.This manuscript reports the fabrication of a highly temperature-sensitive semi-interpenetrating optical hydrogel fiber(TSOHF)using the integrated dynamic wet-spinning technique.TSOHF exhibits a structural tunable diameter,clear core/sheath structure,tunable temperature-sensitivity,excellent light propagation property(0.35 dB cm^(-1),650 nm laser light),and good biocompatibility(including tissue-like Young’s modulus,stable dimensional stability,and low cytotoxicity).Based on these properties,a potential application of optogenetic regulation of neural tissue(hypoglossal nerve),with controllable temperature using TSOHF was designed and performed.Further,this work provides new insight into molecular design and a practical approach to continually manufacture a temperature-sensitive hydrogel optical fiber for applications in intelligent photomedicine. | Guoyin Chen Siming Xu Qiangqiang Zhou Yuejiao Zhang Yuhan Song Jing Mi Yuehua Liu Kai Hou Jie Pan | 2023 | Advanced Fiber Materials2023,5,3: | 0 |
| 6 | Analysis on Sequence and Elements of Cucumisin Promoter显示文摘Leaves of melon were collected and extracted by the CTAB method for total DNA which was used for PCR amplification,obtaining the gene sequence of cucumisin promoter.The sequence results were processed and analyzed with DNAman,DNAstar and other softwares,and bioinformatic element analysis was performed with PlantCARE and PLACE.The analysis results showed that the cucumisin promoter shared 100%,99% and 99% homology with AY055805,LN713264 and LN681897,respectively.The promoter sequence contains a variety of cis-acting elements common in fruit promoters of higher plants such as TATA-Box and CAAT-Box,and light-responsive elements,some of which involved in ABA and VP1 responsiveness and salicylic acid responsiveness.This study provides a scientific basis for further research on genetic engineering of fruits. | Siming HOU Lingyun LIU Xiaohong YANG | 2017 | Agricultural Biotechnology2017,6,2: | 0 |
| 7 | Cloning and Sequence Analysis of Polygalacturonase(PG) Promoter in Tomato显示文摘In this study,1 500 bp PG gene promoter was amplified from leaves of tomato cultivar‘Zhongshu No.4'.The bioinformatic analysis of PG promoter sequences was conducted.PG promoter elements were predicted and analyzed by PLANTCARE and PLACE.The results showed that tomato PG promoter contained multiple cis-acting regulatory elements such as typical basic elements TATA-Box and CAAT-Box,light responsive elements 3-AF1 binding site,AT1-motif,ATCTmotif,Box 4,Box I,GA-motif,GT1-motif,Sp1 and MRE,heat stress-responsive element HSE,ethylene-responsive element ERE,meristem-specific regulatory element CCGTCC-box,endosperm expression-related regulatory elements GCN4_motif and Skn-1_motif,defense and stress responsiveness element TC-rich repeats,and circadian control-related element circadian,indicating that the expression of tomato PG gene is related to light,temperature,hormone,stress and other factors.This study laid the foundation for subsequent research about regulation of PG gene expression in plants. | Siming HOU Liying ZHOU Lulu BU Chunlei YANG Ting GAO Tian TIAN Zheng'an YANG | 2016 | Agricultural Biotechnology2016,5,2: | 0 |
| 8 | Factors acting on the distribution region of Carlemanniaceae:Rainfall,temperatureand the plants' biological characteristics显示文摘在东亚和热带亚洲的家庭 Carlemanniaceae,包括二个类, Carlemannia 和 Silvianthus,主要沿着 Himalaya 山的南部的脚和云南高原的西方、南部的接口被散布。为 Carlemannia 四角形的主要繁殖过程一被授粉实验在这个工作作为自花受粉验证。为两 C 的种子的萌芽率。当种子在二个月以后是镇定的时, and Silvianthus bracteatus 能百分之百到达的四角形,当率减少了到 25% 时当收集发生在上一年时。种子没有休眠阶段,这被发现,并且他们的种子银行具有短暂土壤类型。在地观察,为水果的裂开地位证明他们的种子不能被囊为疏开有效地逐出。在从年分析温度和降雨数据以后 1971~2000 ,它被看那年度降雨,最低吝啬的月刊一年里的温度,和在一个月内的极端最低温度在 Carlemanniaceae 的分发范围上有大效果,当吝啬的年度温度有更小的效果时。Carlemanniaceae 的狭窄的分发范围被多重因素影响,例如短花粉疏开距离和短暂土壤种子银行。Carlemanniaceae 的人口尺寸被降雨的水平,不是由任何单个变量容易也影响。 | YANG Xue LU ShuGang ZHANG YiPing PENG Hua HOU SiMing | 2007 | Chinese Science Bulletin2007,52,A02: | 0 |