2篇
您的检索式：作者名="Shuning Cai"
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| 1 | Rapid isolation and immune profiling of SARS-CoV-2 specific memory B cell in convalescent COVID-19 patients via LIBRA-seq显示文摘B cell response plays a critical role against SARS-CoV-2 infection.However,little is known about the diversity and frequency of the paired SARS-CoV-2 antigen-specific BCR repertoire after SARS-CoV-2 infection.Here,we performed single-cell RNA sequencing and VDJ sequencing using the memory and plasma B cells isolated from five convalescent COVID-19 patients,and analyzed the spectrum and transcriptional heterogeneity of antibody immune responses.Via linking BCR to antigen specificity through sequencing(LIBRA-seq),we identified a distinct activated memory B cell subgroup(CD11c^(high) CD95^(high))had a higher proportion of SARS-CoV-2 antigen-labeled cells compared with memory B cells.Our results revealed the diversity of paired BCR repertoire and the non-stochastic pairing of SARS-CoV-2 antigen-specific immunoglobulin heavy and light chains after SARS-CoV-2 infection.The public antibody clonotypes were shared by distinct convalescent individuals.Moreover,several antibodies isolated by LIBRA-seq showed high binding affinity against SARS-CoV-2 receptor-binding domain(RBD)or nucleoprotein(NP)via ELISA assay.Two RBD-reactive antibodies C14646P3S and C2767P3S isolated by LIBRA-seq exhibited high neutralizing activities against both pseudotyped and authentic SARS-CoV-2 viruses in vitro.Our study provides fundamental insights into B cell response following SARS-CoV-2 infection at the single-cell level. | Bing He Shuning Liu Yuanyuan Wang Mengxin Xu Wei Cai Jia Liu Wendi Bai Shupei Ye Yong Ma Hengrui Hu Huicui Meng Tao Sun Yanling Li Huanle Luo Mang Shi Xiangjun Du Wenjing Zhao Shoudeng Chen Jingyi Yang Haipeng Zhu Yusheng Jie Yuedong Yang Deyin Guo Qiao Wang Yuwen Liu Huimin Yan Manli Wang Yao-Qing Chen | 2021 | Signal Transduction and Targeted Therapy2021，6，6： | 2 |
| 2 | Enhancing single-molecule conductance of platinum (Ⅱ) complexes through synergistic aromaticity-assisted structural asymmetry显示文摘Seeking the strategies of designing highly conductive molecular structures is one of the core researches in molecular electronics.As asymmetric structure has manifested feasible properties in comprehensive fields, we introduce the structures of asymmetric platinum(Ⅱ) complexes into the charge transport study at single-molecule scale for the first time. The single-molecule conductance measurement results reveal that, in platinum(Ⅱ)-aryloligoynyl structures, the conductance of asymmetrically coordinated complexes is obviously higher than that of the symmetric isomers with the same molecular length, while the conductance is almost identical in symmetric and asymmetric platinum(Ⅱ)-oligoynyl complexes. Theoretical study uncovers that, upon connecting to the oligoynyl structure, the aromatic group effectively extends the π-system of the whole conductive backbone and gathers the HOMO population mainly on the longer oligoynyl ligand, which reduces the energy barrier in electron transport and enhances the conductance through HOMO energy lifting. This result provides feasible strategy for achieving high conductive molecular devices. | Ping Duan Junyang Liu Jin-Yun Wang Kai Qu Shuning Cai Fei Wang Lichuan Chen Xiaoyan Huang Ruihao Li Jia Shi Qian-Chong Zhang Wenjing Hong Zhong-Ning Chen | 2020 | Science China Chemistry2020，63，4： | 0 |
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