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| 1 | Effects of methotrexate on the developments of heart and vessel in zebrafish显示文摘Methotrexate (MTX ) , folic 酸的一个对手,能禁止在 tetrahydrofolic 酸和胚胎的开发的合成很重要的 dihydrofolate reductase (DHFR ) 。在这研究,我们在暴露于 1.5 以后发现了那 ? 在 610 个小时的公里 MTX 授精以后, zebrafish 胚胎没能形成正常心血管的系统。在对待 MTX 的胚胎,室和中庭的词法发展被破坏,心脏的扭曲是反常的,心率和室的弄短部分被减少,并且脉管的发展被破坏。我们也与钙盐五水合物(CF ) 能在对待 MTX 的胚胎在心和容器引起改进开发的 folinic 酸与 dhfrgfp mRNA 或处理发现那任何一个 microinjection,它证明那 MTX 由禁止 DHFR 导致了畸形性。象 hand2, mef2a, mef2c,和 flk-1 那样的基因的抄本层次在对待 MTX 的胚胎被减少。与对待 MTX 的组相比, hand2, mef2a, mef2c,和 flk-1 的抄本层次在 MTX + dhfrgfp 被增加注射 mRNA 的组并且在 MTX + CF 组。我们的结果显示在对待 MTX 的胚胎的心和容器的破坏发展与 hand2, mef2a, mef2c,和 flk-1 的减少的抄本层次有关。 | Shuna Sun Yonghao Gui Yuexiang Wang Linxi Qian Xuefei Liu Qiu Jiang Houyan Song | 2009 | Acta Biochimica et Biophysica Sinica2009，41，1： | 10 |
| 2 | Quantitative analysis of the ribbon synapse number of cochlear inner hair cells in C57BL/6J mice using the three-dimensional modeling method显示文摘In mammals,the ribbon synapses of cochlear inner hair cells are a synaptic structure of the first sensory nerve in the pathway of acoustical signal transmission to the acoustic center,and it is directly involved in voice coding and neurotransmitter release. It is difficult to quantitatively analyze the ribbon synaptic number only using an electron microscope,because the ribbon synaptic number is relatively limited and their location is deep. In this study,the specific presynaptic structure-RIBEYE,and non-specific postsynaptic structure-GluR 2 & 3 in C57BL/6J mouse basilar membrane samples were treated by immunofluorescent labeling. Serial section was performed on the samples using a laser scanning confocal microscope,and then the serial sections were used to build three-dimensional models using 3DS MAX software. Each fluorescein color pair indicates one synapse,so the number of ribbon synapses of inner hair cells is obtained. The spatial distribution and the number of ribbon synapses of cochlear inner hair cells were clearly shown in this experiment,and the mean number of ribbon synapses per inner hair cell was 16.10±1.03. Our results have demonstrated the number of ribbon synapses is accurately calculated by double immunofluorescent labeling to presynaptic and postsynaptic structures,serial sections obtained using a laser scanning confocal microscope,and three-dimensional modeling obtained using 3DS MAX software. The method above is feasible and has important significance for further exploring the mechanism of sensorineural deafness. | LIU Ke,LI ShuNa & JIANG XueJun Department of Otolaryngology,the First Affiliated Hospital,China Medical University,Shenyang 110001,China | 2009 | Science China(Life Sciences)2009，52，9： | 5 |
| 3 | Extranodal involvement in young patients with diffuse large B-cell lymphoma: distribution, prognostic value and treatment options显示文摘Objective: Extranodal involvement represents a peculiar presentation of diffuse large B-cell lymphoma(DLBCL). Previous studies have suggested that older patients are more prone to extranodal involvement. This study retrospectively addressed the distribution, prognostic value and treatment options of extranodal involvement in young patients with DLBCL.Methods: A total of 329 patients were enrolled according to the inclusion requirements. The effects of gender,extranodal involvement, age-adjusted international prognostic index(aa IPI), rituximab infusion and radiotherapy on patient outcomes were evaluated.Results: Among these patients, 59% presented extranodal involvement in 16 anatomic sites. More than one instance was linked to many poorer clinical characteristics and poorer survival compared with either nodal disease or one instance. In patients with one extranodal lesion, multivariate analysis revealed that the site of extranodal involvement, but not the aa IPI or rituximab infusion, was independently related to the outcome, and radiotherapy had a negative influence on survival.Conclusions: Extranodal involvement is common in younger patients and exhibits a ubiquitous distribution.The site of extranodal involvement is of strong prognostic significance. Radiotherapy for extranodal lesions does not improve patient outcomes. | Shuna Yao Junbo Li Zhihua Yao Yuanlin Xu Junfeng Chu Jiuyang Zhang Shuiling Jin Yangyang Huang Jianbo Zhang Jie Ma Yan Zhao Shujun Yang Yanyan Liu | 2017 | Chinese Journal of Cancer Research2017，29，1： | 3 |
| 4 | Modification of physicochemical properties and degradation of barley flour upon enzymatic extrusion显示文摘In order to evaluate the application potential of enzyme-extruded barley flour(EEBF)in the brewing of whole-barley beer,the physicochemical properties,mashing and fermentation effects of EEBF were studied,and conventional extruded barley flour(CEBF)and steam-cooked barley flour(SCBF)were taken as controls.The water solubility index(WSI)of EEBF(46.88%-52.63%)was much higher than that of CEBF(6.70%)and SCBF(4.59%).The results of degree of gelatinization(DG)and enthalpy(ΔH)suggested that EEBF was almost completely gelatinized,and the rapid visco analyzer(RVA)exhibited that the peak viscosity of EEBF was drastically reduced.The weight-average molecular weight of EEBF(<10^(6) g/mol)was significantly lower than that of CEBF and SCBF.Scanning electron microscopy(SEM)showed that the heat-treatments caused starch granules to break and aggregate,and there were many hydrolyzed pores on the surface of EEBF.Both the long-and short-range order of EEBF were destroyed.Although EEBF caused serious filtration blocking,it greatly improved the alcohol content of finished beer(5.41%vol).Overall,the thermal modification of barley flour by enzyme-extrusion meets the needs of fermentation,and could improve the problem of limited leaching of unmalted barley,which was benefit for the production of whole-barley beer. | Shuna Zhao Aiquan Jiao Yueyue Yang Qing Liu Wenqi Wu Zhengyu Jin | 2022 | Food Bioscience2022，45，1： | 2 |
| 5 | Single-walled carbon nanotubes functionalized with poly(nile blue A) and their application to dehydrogenase-based biosensors显示文摘 | Pan Du Shuna Liu Ping Wu Chenxin Cai | 2007 | 53:1811-18232007，53，： | 1 |
| 6 | Single-walled carbon nanotubes functionalized with poly( nile blue A) and their application to dehydrogenase-based biosensors显示文摘 | DU Pan LIU Shuna WU Ping | 2007 | Electrochim Acta2007，53，4： | 1 |
| 7 | Programmable deaminase-free base editors for G-to-Y conversion by engineered glycosylase显示文摘Current DNA base editors contain nuclease and DNA deaminase that enables deamination of cytosine(C)or adenine(A),but no method for guanine(G)or thymine(T)editing is available at present.Here we developed a deaminase-free glycosylase-based guanine base editor(gGBE)with G editing ability,by fusing Cas9 nickase with engineered N-methylpurine DNA glycosylase protein(MPG).By several rounds of MPG mutagenesis via unbiased and rational screening using an intron-split EGFP reporter,we demonstrated that gGBE with engineered MPG could increase G editing efficiency by more than 1500 fold.Furthermore,this gGBE exhibited high base editing efficiency(up to 81.2%)and high G-to-T or G-to-C(i.e.G-to-Y)conversion ratio(up to 0.95)in both cultured human cells and mouse embryos.Thus,we have provided a proof-of-concept of a new base editing approach by endowing the engineered DNA glycosylase the capability to selectively excise a new type of substrate. | Huawei Tong Nana Liu Yinghui Wei Yingsi Zhou Yun Li Danni Wu Ming Jin Shuna Cui Hengbin Li Guoling Li Jingxing Zhou Yuan Yuan Hainan Zhang Linyu Shi Xuan Yao Hui Yang | 2023 | National Science Review2023，10，8： | 1 |
| 8 | Preparation of Peanut Shell Cellulose Double-Network Hydrogel and Its Adsorption Capacity for Methylene Blue显示文摘To achieve optimal recovery and value-added utilisation of cellulose in peanut shells,the cellulose in peanut shells was first extracted using the sodium hydroxide-sodium chlorite method.Then,cellulose hydrogel was prepared by graft copolymerisation using N,N’-methylenebisacrylamide as the cross-linking agent,sodium persulfate as the initiator,and acrylic acid as the monomer.Orthogonal optimisation experiments were designed to obtain optimal process parameters for hydrogel preparation with the cellulose dosage of 0.40 g,initiator dosage of 0.20 g,polymerisation temperature of 70°C,cross-linking agent of 0.25 g,and monomer dosage of 3.0 mL.The effect of initiator dosage on hydrogel synthesis was the most significant,followed by monomer dosage and reaction temperature.Characterisation using X-ray diffraction analysis and scanning electron microscopy revealed that the hydrogel was amorphous and exhibited a distinct three-dimensional double network structure.Hydrogel swelling kinetic analysis showed that the hydrogel swelling process was divided into three stages,and fitted the Schott secondary swelling kinetic model.The prepared hydrogel had a good adsorption effect on methylene blue;the adsorption of methylene blue by the hydrogel was 1.259 mg/g at 25°C when the initial concentration of methylene blue was 5 mg/L.The adsorption kinetics of the hydrogel fit the pseudo-first-order kinetic model,pseudo-secondorder kinetic model,Eovich model and particle diffusion model.The best fitting effect was obtained with the pseudo-second-order kinetic model.The adsorption isotherm analysis of methylene blue on hydrogel showed that the adsorption process was consistent with Langmuir and Freundlich models.The correlation coefficient of the Freundlich isotherm model was higher,indicating that the adsorption of methylene blue on hydrogel was mainly chemisorption. | Yalin Li Lei Liu Wenbao Huang Junpu Xie Zhaoxia Song Shuna Guo Enci Wang | 2023 | Journal of Renewable Materials2023，11，7： | 0 |
| 9 | Interfacial synergistic effect in SnO_(2)/PtNi nanocrystals enclosed by high-index facets for high-efficiency ethylene glycol electrooxidation显示文摘Strengthening the oxide-metal interfacial synergistic interaction in nanocatalysts is identified as potential strategy to boost intrinsic activities and the availability of active sites by regulating the surface/interface environment of catalysts.Herein,the SnO_(2)/PtNi concave nanocubes(CNCs)enclosed by high-index facets(HIFs)with tunable SnO_(2)composition are successfully fabricated through combining the hydrothermal and self-assembly method.The interfacial interaction between ultrafine SnO_(2)nanoparticles and PtNi with HIFs surface structure is characterized by analytical techniques.The as-prepared 0.20%SnO_(2)/PtNi catalyst exhibits extraordinarily high catalytic performance for ethylene glycol electrooxidation(EGOR)in acidic conditions with specific activity of 3.06 mA/cm^(2),which represents 6.2-fold enhancement compared with the state-of-the-art Pt/C catalyst.Additionally,the kinetic study demonstrates that the strong interfacial interaction between SnO_(2)and PtNi not only degrades the activation energy barrier during the process of EGOR but also enhances the CO-resistance ability and long-term stability.This study provides a novel perspective to construct highly efficient and stable electrocatalysts for energy conversions. | Shuna Li Haixiao Sun Jiaai Zhang Longjiao Zheng Yunrui Li Xu Fang Yujie Liu Qi Song Zhen Wang Yufeng Gao Xin Zhang Xiaoping Dai Yandi Cai Fei Gao | 2022 | Nano Research2022，15，9： | 0 |
| 10 | Psychological and Emotional Responses during Different Stages of the COVID-19 Pandemic Based on a Survey of a Mental Health Hotline显示文摘Background:The coronavirus(COVID-19)outbreak in 2019 triggered psychological and emotional responses.This research investigates the psychological status and emotional problems of those who sought psychological assistance during the epidemic period by calling a mental health hotline.Methods:This study aims to combine qualitative and quantitative research.Descriptive analysis was used for undertaking qualitative research.We ana-lyzed the data from group 1(n=706),in which the people used the mental health hotline from 25 January 2020 to 23 June 2020.A self-designed questionnaire was developed in accordance with the classification and sum-marized items from group 1’s psychological problems and emotional status.To implement the quantitative research,we conducted a cross-sectional descriptive survey and used the self-compiled scale and HADS to inves-tigate group 2(n=553)from May 2020 to June 2020.Results:Descriptive statistics and comparative analysis revealed that:①Visitors mainly reported behavior,emotional,family relationship problems and sleep disorders.②Anxiety,comorbidities,sleep disorders and coping problems were the most frequently reported problems.③There were significant differences in the number of visitors experiencing various problems or exhibiting harmful behaviors(sorrow,worry,fear,depression,sleep disorders,self-harm or suicide,and coping problems,anxiety,hypochondria,and comorbidity)in the four stages of the epidemic.④More than a quarter of participants still suffered from anxiety or depression in the later stages of the epidemic.Conclusion:Different problems manifested at different stages of the epidemic,and psychological interventions and assistance should be tailored to reflect this. | Shuna Peng Xiaohong Luo Shiyu Liang Fengning Deng Yuning Liu Hong Zeng Xuesong Yang | 2022 | International Journal of Mental Health Promotion2022，24，5： | 0 |
| 11 | Aqueous synthesis of bright near-infrared-emitting Zn-Cu-In-Se quantum dots for multiplexed detection of tumor markers显示文摘Aqueous synthesis is a green but challenging pathway to produce cadmium-free,bright,and near-infrared(NIR)emitting quantum dots(QDs).Herein,we develop a straightforward aqueous phase approach to prepare Zn-Cu-In-Se QDs with tunable emission from 630 to 800 nm through varying the molar ratio of Zn and Cu.The ultrasmall nanoparticle size remains nearly fixed(~2.6 nm)when the QD composition is varied,allowing for the effects of composition on the optical properties of QDs to be decoupled from the impact of particle size.By leveraging the off-stoichiometric effect on photoluminescence(PL),Zn-Cu-In-Se QDs with a Zn:Cu molar ratio of 1.0 show a long PL decay lifetime of 211 ns and an absolute photoluminescence quantum yield(PLQY)of 14.2%.The PLQY is further enhanced by overcoating a ZnS shell,reaching up to 25.8%.Based on the bright,biocompatible,and emission-tunable QDs,nanoprobes with targeting capability for multiple tumor markers are further constructed and employed to simultaneously ascertain targets in the cytoplasm and cell membrane.This study provides a green and effective strategy for achieving bright and biocompatible NIR quantum dots for multiplexed biodetection applications. | Hui Liu Peng Cai Kevin J.McHugh Collin F.Perkinson Laishuo Li Shuna Wang Wei Wang Mingxia Jiao Xiliang Luo Lihong Jing | 2022 | Nano Research2022，15，9： | 0 |
| 12 | Epidemiological,Clinical and Serological Characteristics of Children with Coronavirus Disease 2019 in Wuhan:A Single-centered,Retrospective Study显示文摘Dear Editor,In December 2019,SARS-Co V-2 was first detected in the samples obtained from three adult patients who suffered from an unknown viral pneumonia in Wuhan(Li et al.2020).This unknown viral pneumonia is further named as coronavirus disease 2019(COVID-19)by the World Health Organization. | Dan Luo Zhi Xia Heng Li Danna Tu Ting Wang Wei Zhang Lu Peng Wenfu Yi Sai Zhang Junhua Shu Hui Xu Yong Li Buyun Shi Chengjiao Huang Wen Tang Shuna Xiao Xiaolan Shu Yan Liu Yuan Zhang Shan Guo Zhi Yu Baoxiang Wang Yuan Gao Qinxue Hu Hanzhong Wang Xiaohui Song Hong Mei Xiaoqin Zhou Zhenhua Zheng | 2020 | Virologica Sinica2020，35，6： | 0 |
| 13 | PtNiCu nanowires with advantageous lattice-plane boundary for enhanced ethanol electrooxidation显示文摘It is generally accepted that the interface effect and surface electronic structure of catalysts have vital impact on catalytic properties.Understanding and tailoring the atomic arrangement of interface structure are of great importance for electrocatalysis.Herein,we proposed a simple method to synthesize etching-PtNiCu nanowires(e-PtNiCu NWs)enclosed by both(110)and(100)facets evolving from PtNiCu nanowires(PtNiCu NWs)mainly with(111)facets by selectively etching process.After acetic acid etching treatment,the e-PtNiCu NWs possess high total proportions(88.3%)of(110)and(100)facets,whereas the(111)facet is dominant in PtNiCu NWs(64%)by qualitatively and quantitatively evaluation.Combining the structure characterizations and performance tests of ethanol electrooxidation reaction(EOR),we find that the e-PtNiCu NWs display remarkably performance for EOR,which is nearly 4.5 times and 1.5 times enhancement compared with the state-of-the-art Pt/C catalyst,as well as 2.2 and 1.4 times of PtNiCu NWs,in specific activity and mass activity,respectively.The improved performance of e-PtNiCu NWs is attributed to synergistic catalytic effect between(110)and(100)facets that not only significantly decreases the onset potentials of adsorbed CO(CO_(ads))but also favors the oxidation of CO_(ads)on the surface of catalyst.Furthermore,thermodynamics and kinetic studies indicate that the synergistic effect of both(110)and(100)facets in e-PtNiCu NWs can decrease the activation energy barrier and facilitate the charge transfer during the reaction.This work provides a promising approach to construct catalysts with tunable surface electronic structure towards efficient electrocatalysis. | Shuna Li Yao Wang Yunrui Li Xu Fang Yujie Liu Mingxuan Li Zhen Wang Yufeng Gao Haixiao Sun Fei Gao Xin Zhang Xiaoping Dai | 2022 | Nano Research2022，15，4： | 0 |
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