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| 1 | A Tailored High-Efficiency Sample Pretreatment Method for Simultaneous Quantification of 10 Classes of Known Endogenous Phytohormones显示文摘One of the hottest topics in plant hormone biology is the crosstalk mechanisms,whereby multiple classes of phytohormones interplay with each other through signaling networks.To better understand the roles of hormonal crosstalks in their complex regulatory networks,it is of high significance to investigate the spatial and temporal distributions of multiple-phytohormones simultaneously from one plant tissue sample.In this study,we develop a high-sensitivity and high-throughput method for the simultaneous quantitative analysis of 44 phytohormone compounds,covering currently known 10 major classes of phytohormones(strigolactones,brassinosteroids,gibberellins,auxin,abscisic acid,jasmonic acid,salicylic acid,cytokinins,ethylene,and polypeptide hormones[e.g.,phytosulfokine])from only 100 mg of plant sample.These compounds were grouped and purified separately with a tailored solid-phase extraction procedure based on their physicochemical properties and then analyzed by LC–MS/MS.The recoveries of our method ranged from 49.6%to 99.9%and the matrix effects from 61.8%to 102.5%,indicating that the overall sample pretreatment design resulted in good purification.The limits of quantitation(LOQs)of our method ranged from 0.06 to 1.29 pg/100 mg fresh weight and its precision was less than 13.4%,indicating high sensitivity and good reproducibility of the method.Tests of our method in different plant matrices demonstrated its wide applicability.Collectively,these advantages will make our method helpful in clarifying the crosstalk networks of phytohormones. | Peiyong Xin Qiuhuan Guo Bingbing Li Shujing Cheng Jijun Yan Jinfang Chu | 2020 | Plant Communications2020，1，3： | 7 |
| 2 | Medicinal chemistry strategies towards the development of effective SARS-CoV-2 inhibitors显示文摘Novel therapies are urgently needed to improve global treatment of SARS-CoV-2 infection.Herein,we briefly provide a concise report on the medicinal chemistry strategies towards the development of effective SARS-CoV-2 inhibitors with representative examples in different strategies from the medicinal chemistry perspective. | Shenghua Gao Tianguang Huang Letian Song Shujing Xu Yusen Cheng Srinivasulu Cherukupalli Dongwei Kang Tong Zhao Lin Sun Jian Zhang Peng Zhan Xinyong Liu | 2022 | Acta Pharmaceutica Sinica B2022，12，2： | 5 |
| 3 | Creation of fragrant sorghum by CRISPR/Cas9显示文摘Sorghum,the fifth largest cereal crop,has high value as a staple food and raw material for liquor and vinegar brewing.Due to its high biomass and quality,it is also used as the second most planted silage resource.No fragrant sorghums are currently on the market.Through CRISPR/Cas9-mediated knockout of Sb BADH2,we obtained sorghum lines with extraordinary aromatic smell in both seeds and leaves.Animal feeding experiments showed that fragrant sorghum leaves were attractable.We believe this advantage will produce great value in the sorghum market for both grain and whole biomass forage. | Dan Zhang Sanyuan Tang Peng Xie Dekai Yang Yaorong Wu Shujing Cheng Kai Du Peiyong Xin Jinfang Chu Feifei Yu Qi Xie | 2022 | Journal of Integrative Plant Biology2022，64，5： | 1 |
| 4 | Design, Syntheses and Biological Activities of Novel Sulfonylureas Containing an Oxime Ether Moiety显示文摘 | WEI Wei CHENG Dandan CHEN Wei LIU Jingbo WAN Yingying LI Yuxin LI Yonghong YU Shujing LI Zhengming | 2016 | Chemical Research in Chinese Universities2016，32，2： | 1 |
| 5 | Design, Synthesis and Biological Activities of Novel 4,5-Disubstituted-3-S-(β-D-acetylglycosyl)- 1,2,4-triazole Derivatives显示文摘 | CHENG Dandan WEI Wei LI Yuxin HUA Xuewen ZHOU Yunyun LIU Jingbo LI Yonghong YU Shujing XIONG Lixia LI Zhengming | 2017 | Chemical Research in Chinese Universities2017，33，5： | 0 |
| 6 | SnoRNP is essential for thermospermine-mediated development in Arabidopsis thaliana显示文摘Polyamines have been discovered for hundreds of years and once considered as a class of phytohormones.Polyamines play critical roles in a range of developmental processes.However,the molecular mechanisms of polyamine signaling pathways remain poorly understood.Here,we measured the contents of main types of polyamines,and found that endogenous level of thermospermine(T-Spm)in Arabidopsis thaliana is comparable to those of classic phytohormones and is significantly lower than those of putrescine(Put),spermidine(Spd),and spermine(Spm).We further found a nodule-like structure around the junction area connecting the shoot and root of the T-Spm biosynthetic mutant acl5 and obtained more than 50 suppressors of acl5 nodule structure(san)through suppressor screening.An in-depth study of two san suppressors revealed that NAP57 and NOP56,core components of box H/ACA and C/D snoRNPs,were essential for T-Spm-mediated nodule-like structure formation and plant height.Furthermore,analyses of rRNA modifications showed that the overall levels of pseudouridylation and 2′-O-methylation were compromised in san1 and san2 respectively.Taken together,these results establish a strong genetic relationship between rRNA modification and T-Spm-mediated growth and development,which was previously undiscovered in all organisms. | Xilong Li Zongyun Yan Meiling Zhang Jiayin Wang Peiyong Xin Shujing Cheng Liquan Kou Xiaoting Zhang Songlin Wu Jinfang Chu Chengqi Yi Keqiong Ye Bing Wang Jiayang Li | 2023 | Science China(Life Sciences)2023，66，1： | 0 |
| 7 | A sensitive and accurate method for quantifying endogenous systemin levels and verifying natural occurrence of Leu-Systemin显示文摘Dear Editor,Tomato systemin(TomSys),the first discovered plant peptide hormone,can initiate systemic mobile signals to stimulate an immune response in both wounded and intact leaves(Pearce et al.,1991).For decades,biologists have been striving to uncover the mechanisms of TomSys function,but detailed information on the TomSys-regulated systemic defense process remains limited to date.The specific roles of three potential transmissible signaling components,ProSys mRNA,TomSys,and jasmonic acid(JA),in activation of systemic defense responses still need to be clarified(Zhang et al.,2020). | Jijun Yan Peiyong Xin Shujing Cheng Jinfang Chu | 2023 | Plant Communications2023，4，5： | 0 |
| 8 | Mechanism of mitochondrial protection by the Buyin Qianzheng formula in a Parkin overexpression cell model显示文摘Objective:To identify the molecular mechanisms of the effects of the Buyin Qianzheng formula(BYQZF)on the mitochondrial dynamics in a Parkin overexpression Parkinson's disease(PD)cell model.Methods:First,a stable Parkin overexpression cell model was constructed using plasmid transfection.Then,we examined the protective effect of BYQZF on the mitochondrial dysfunction of the Parkin overexpression PD cell model induced by neurotoxin 1-methyl-4-phenylpyridinium ion(MPPþ).The mRNA expression level of Parkin was evaluated using real-time quantitative PCR.The cell survival rate was detected using the Cell Counting Kit-8 assay.We evaluated the cellular adenosine triphosphate(ATP)levels using luciferase assays.A laser scanning confocal microscope was used to observe the mitochondrial morphology,activity,and mitochondrial membrane potential(DJm).Western blot was conducted to evaluate the levels of the fusion proteins mitofusin1,mitofusin2,optic atrophy 1,dynaminrelated protein 1,and mitochondrial fission protein 1.Results:Parkin overexpression attenuated MPPþ-induced mitochondrial damage,increased mitochondrial activity and DJm.BYQZF increased the survival of MPPþ-induced cells that overexpressed Parkin and upregulated the mitochondrial form factor and activity.It also inhibited a decrease in the DJm and ATP levels.These findings suggested that BYQZF protected against MPPþ-induced mitochondrial dysfunction and enhanced the protective effect of Parkin overexpression.Furthermore,the formula upregulated the expression of the fusion proteins mitofusin1,mitofusin2,and optic atrophy 1(closely related to mitochondrial quality remodeling),and reduced the expression of the fission protein dynamicrelated protein 1,as well as mitochondrial fission protein 1.Conclusion:The mechanism by which BYQZF increased the mitochondrial protective effect of Parkin gene overexpression in MPPþ-induced cells may be related to improving mitochondrial function and regulating the balance of mitochondrial division and fusion proteins. | Cuicui Cheng Yushan Gao Cong Gai Wandi Feng Haojie Ma Jing Feng Zhenyu Guo Shujing Zhang Jie Wu Hongmei Sun | 2022 | Journal of Traditional Chinese Medical Sciences2022，9，1： | 0 |
| 9 | Ultrafiltration of Shiitake Polyphenol with Ultrasound Enhancement显示文摘Ultrafiltration(UF) experiments were subjected to the concentration of Shiitake polyphenol(SP) with ultrasound(US) enhancement, by which the effects of the main parameters such as ultrasonic power, transmembrane pressure, temperature and axial flow-rate on the membrane fluxes were analyzed, and then the SP of the ultrafiltrate after follow-up resin adsorption by HPLC was investigated. The results indicated that US could significantly enhance the membrane fluxes. The optimal conditions obtained was as follows: 0.60 W/cm2 US power, axial flow-rate of 80 L/h,operation temperature 30 ℃, transmembrane pressure 0.10 MPa. US increased the absorption capacity of the absorbent resin during the following stage. The HPLC analysis also showed that catechins were purified to some extent as the ultrafiltrate was treated by US and macroporous resin, indicating the technology of US enhancement coupled with UF showed the potential for concentration and purification of SP by absorbent resins. | Defang SHI Hong GAO Xiuzhi FAN De YANG Shujing XUE Wei CHENG | 2015 | Agricultural Science & Technology2015，16，10： | 0 |
| 10 | Rectal Cancer Stages T2 and T3 Identification Based on Asymptotic Hybrid Feature Maps显示文摘Many existing intelligent recognition technologies require huge datasets for model learning.However,it is not easy to collect rectal cancer images,so the performance is usually low with limited training samples.In addition,traditional rectal cancer staging is time-consuming,error-prone,and susceptible to physicians’subjective awareness as well as professional expertise.To settle these deficiencies,we propose a novel deep-learning model to classify the rectal cancer stages of T2 and T3.First,a novel deep learning model(RectalNet)is constructed based on residual learning,which combines the squeeze-excitation with the asymptotic output layer and new cross-convolution layer links in the residual block group.Furthermore,a two-stage data augmentation is designed to increase the number of images and reduce deep learning’s dependence on the volume of data.The experiment results demonstrate that the proposed method is superior to many existing ones,with an overall accuracy of 0.8583.Oppositely,other traditional techniques,such as VGG16,DenseNet121,EL,and DERNet,have an average accuracy of 0.6981,0.7032,0.7500,and 0.7685,respectively. | Shujing Sun Jiale Wu Jian Yao Yang Cheng Xin Zhang Zhihua Lu Pengjiang Qian | 2023 | Computer Modeling in Engineering & Sciences2023，，10： | 0 |
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