|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | β-elemene enhances the radiosensitivity of gastric cancer cells by inhibiting Pak1 activation显示文摘AIM:To explore the potential of β-elemene as a radiosensitizer for gastric cancer cells and the underlying mechanisms.METHODS:SGC7901,MKN45,MKN28,N87,and AGS human gastric cancer cell lines were used to screen for radioresistant gastric cancer cell lines. A 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium(MTT) assay was used to determine the effects of β-elemene and IPA-3 on cell viability in MKN45 and SGC7901 gastric cancer cell lines. A clonogenic survival assay and annexin V-FITC/PI apoptosis detection assay were used to evaluate cellular radiosensitivity and radiation-induced cell death,respectively. A proteomic method,isobaric tags for relative and absolute quantitation(i TRAQ),was employed to screen the proteins regulated by β-elemene pretreatment prior to ionizing radiation(IR) in SGC7901 gastric cancer cell line. IPA-3 was used as a specific small molecule inhibitor of p21-activated protein kinase 1(Pak1) to target Pak1 signaling. Protein levels of PAK1IP1(p21-activated protein kinase-interacting protein 1),total Pak1(t-Pak1),phospho-Pak1(T423),phospho-ERK1/2( Thr202/Tyr204),and cleaved caspase-3(17 k Da) were assessed by western blotting.RESULTS:MKN45 and SGC7901 gastric cancer cell lines were relatively more resistant to IR. β-elemene pretreatment decreased clonogenic survival following IR in MKN45 and SGC7901 gastric cancer cell lines. Additionally,β-elemene pretreatment prior to IR increased radiation-induced cell death compared with IR alone in MKN45(10.4% ± 0.9% vs 34.8% ± 2.8%,P < 0.05) and SGC7901(11.6% ± 0.9% vs 46.7% ± 5.2%,P < 0.05) human gastric cancer cell lines,respectively,consistent with the level of cleaved caspase-3(17 k Da). Through i TRAQ analysis and western blot validation,we found that β-elemene upregulated PAK1IP1 and downregulated phospho-Pak1(T423) and phosphoERK1/2 in SGC7901 gastric cancer cells. IR increased the level of phospho-Pak1(T423). Pretreatment with β-elemene decreased radiation-induced Pak1 and ERK1/2 phosphorylation. Inhibition of Pak1 using IPA-3 decreased clonogenic survival following IR. In addition,IPA-3 increased radiation-induced cell death in MKN45(13.4% ± 0.3% vs 26.6% ± 1.0%,P < 0.05) and SGC7901(16.0% ± 0.6% vs 37.3% ± 1.7%,P < 0.05) gastric cancer cell lines,respectively,consistent with the level of cleaved caspase-3(17 k Da). Western blotting showed that IPA-3 decreased radiation-induced Pak1 and ERK1/2 phosphorylation.CONCLUSION:This is the first demonstration that β-elemene enhances radiosensitivity of gastric cancer cells,and that the mechanism involves inhibition of Pak1 signaling. | Jun-Song Liu Xiang-Ming Che Shuai Chang Guang-Lin Qiu Shi-Cai He Lin Fan Wei Zhao Zheng-Liang Zhang Shu-Feng Wang | 2015 | World Journal of Gastroenterology2015，21，34： | 9 |
| 2 | Biogenic silica contents of Lake Qinghai sediments and its environmental significance显示文摘在在湖沉积的源於生物的硅石(BSi%) 的层次的变化广泛地被使用了以便学习湖生产率和 palaeoclimatic 变化。然而,源於生物的硅石(BSi ) 的起源需要为每个湖被调查,特别为大湖,作为做在 BSi 和相关环境因素的层次之间的关系。在这研究,我们测量了在金海湖集水以内在湖沉积,河沉积,和表面土壤包含的 BSi 的百分比,并且在抽取过程前后比较了硅藻和 phytoliths 的数量和形状。结果建议在湖沉积的 BSi 首先从内长的硅藻被导出;因此, BSi 层次能被用来在湖以内在主要生产率反映变化。进一步的比较证明在长期的 timescales 上,在 BSi% 的变化与在全部的器官的碳(TOC ) 和谷物尺寸的那些通常一致,反映在金海湖的主要生产率上的降水的主导的影响。然而,在短期的 timescales 上,在 BSi% 之间的关系和在 BSi% 和谷物尺寸之间的 TOC 和那不清楚或稳定。例如, BSi% 有时有谷物的 covaried 尺寸,而是它与谷物尺寸有关有时从阶段与或甚至相反地。我们推测那气候和环境过程例如冲淡效果,影响短期的 BSi% 和它的相关环境意义。作为结果, BSi% 应该在不同时间规模上有选择地被用作气候的变化的指示物。 | Bin LIU Hai XU Jianghu LAN Enguo SHENG Shuai CHE Xinying ZHOU | 2014 | Frontiers of Earth Science2014，8，4： | 4 |
| 3 | The Effect of Hydrolysis with Neutrase on Molecular Weight,Functional Properties,and Antioxidant Activities of Alaska Pollock Protein Isolate显示文摘In this study, the Alaska pollock protein isolate(APPI) was hydrolyzed by Neutrase for 20, 40, 80, 120, 160, 200, and 240 min. Hydrolysates with different molecular weights were produced and they were named as H1–H7. Furthermore, the effects of hydrolysis on the average molecular weights, functional properties(solubility, oil-holding capacities, foaming activities, and emulsifying properties), and antioxidant activities(1, 1-diphenyl-2-picrylhydrazyl, superoxide, and hydroxyl free radical-scavenging activities) were determined. It was found that when the degree of hydrolysis(DH) increased, the average molecular weights of the hydrolysates decreased significantly. The functional properties of APPI were also significantly improved. The hydrolysates of APPI exhibited better solubility, emulsifying activities, and foaming activities. Hydrolysates with low molecular weights(<1 kDa) had better solubility, oil-holding capacities, and emulsifying activities, while hydrolysates with higher molecular weights(>1 kDa) had better foaming activities. In addition, the hydrolysates exhibited excellent antioxidant properties, while the inhibition values of 1, 1-diphenyl-2-picryl hydroxyl(DPPH), superoxide, and hydroxyl free radical-scavenging activities, were 85.22%, 53.56%, and 75.00% respectively, when the concentration of the hydrolysates was 5.0 mg mL^(-1). The lower the average molecular weight was, the higher was the antioxidant activity. These results indicated that hydrolysis with Neutrase is an effective method for improving the functional and antioxidant properties of APPI. The hydrolysates of APPI displayed great potentials to be used as natural antioxidants in protein-rich aqueous foods such as nutrient supplements and sports beverages. | LIU Chuyi MA Xiaoming CHE Shuai WANG Changwei LI Bafang | 2018 | Journal of Ocean University of China2018，17，6： | 3 |
| 4 | Preparation of zeolite NaA for CO_2 capture from nickel laterite residue显示文摘Zeolite NaA was successfully prepared from nickel laterite residue for the first time via a fusion–hydrothermal procedure. The structure and morphology of the as-synthesized zeolite NaA were characterized with a range of experimental techniques, such as X-ray diffraction, scanning electronic microscopy, and infrared spectroscopy. It was revealed that the structures of the produced zeolites were dependent on the molar ratios of the reactants and hydrothermal reaction conditions, so the synthesis conditions were optimized to obtain pure zeolite NaA. Adsorption of nitrogen and carbon dioxide on the prepared zeolite NaA was also measured and analyzed. The results showed that zeolite NaA could be prepared with reasonable purity, it had physicochemical properties comparable with zeolite NaA made from other methods, and it had excellent gas adsorption properties, thus demonstrating that zeolite NaA could be prepared from nickel laterite residue. | Tao Du Li-ying Liu Penny Xiao Shuai Che He-ming Wang | 2014 | International Journal of Minerals,Metallurgy and Materials2014，21，8： | 2 |
| 5 | Anticancer effects of β-elemene in gastric cancer cells and its potentialunderlying proteins: A proteomic study显示文摘 | Jun-Song Liu Shi-Cai He Zheng-Liang Zhang Rui Chen Lin Fan Guang?Lin Qiu Shuai Chang Liang Li Xiang-Ming Che | 2014 | Oncology Reports2014，，6： | 1 |
| 6 | A performance study of generalpurpose applications on graphics processors using CUDA显示文摘 | Che Shuai Boyer M Meng Jiayuan | 2008 | Journal of Parallel and Distributed Computing2008，68，10： | 1 |
| 7 | A performance study of general-purpose applications on graphics processors using CUDA显示文摘 | Shuai Che Michael Boyer Jiayuan Meng David Tarjan Jeremy W. Sheaffer Kevin Skadron | 2008 | Journal of Parallel and Distributed Computing2008，，10： | 1 |
| 8 | A performance study of general-purpose applications on graphics processors using CUDA显示文摘 | Shuai Che Michael Boyer Jiayuan Meng David Tarjan Jeremy W. Sheaffer Kevin Skadron | 2008 | Journal of Parallel and Distributed Computing2008，，10： | 1 |
| 9 | The tumor rejection effect of protein components from medicinal fungus显示文摘 | Yi Liang Yijie Chen Honghong Liu Rong Luan Tao Che Shuai Jiang Dongyang Xie Hui Sun | 2011 | Biomedicine & Preventive Nutrition2011，，4： | 1 |
| 10 | A performance study of general-purpose applications on graphics processors using CUDA显示文摘 | Che Shuai Boyer M Meng Jiayuan | 2008 | Journal of Parallel and Distributed Computing2008，68，10： | 1 |
| 11 | A performance study of general-purpose applications on graphics processors using CUDA 显示文摘 | CHE Shuai BOYER M MENG Jia - yuan | 2008 | Journal of Parallel and Distributed Computing2008，68，10： | 1 |
| 12 | Improvement of Escape Time Algorithm by No-Escape-Point显示文摘 | Shuai Liu Xiangjiu Che Zhengxuan Wang | 2011 | Journal of Computers2011，，8： | 1 |
| 13 | A performance study of general-purpose applications on graphics processors using CUDA显示文摘 | Che Shuai Boyer M Meng Jiayuan | 2007 | Journal of Parallel and Distributed Computing2007，68，10： | 1 |
| 14 | Experimental study on influence of operating parameters on tar components from corn straw gasification in fluidized bed显示文摘Gasification is a promising approach for converting solid fuel sources, including renewable ones like biomass, for use. The main problem in biomass gasification is the formation of condensable tars, including polycyclic aromatic hydrocarbons (PAHs). This paper investigated the conversion of tar components during corn straw gasification. It analyzed collected tar components using a gas chromatograph-mass spectrograph (GC-MS). Experimental results indicate that, with increasing temperature from 700°C to 900°C, the concentrations of benzene, indene, phenanthrene, naphthalene, acenaphthylene, fluorene, and pyrene increased whereas those of toluene, phenol, 1-methylnaphthalene, and 2-methylnaphthalene decreased. As the equivalence ratio (ER) increased from 0.21 to 0.34, the concentrations of indene and phenanthrene increased from 0.148% and 0.087% to 0.232% and 0.223%, respectively. Further, the phenol content increased as ER increased from 0.21 to 0.26 and then decreased as the ER increased further to 0.34. Other parameters like the steam/biomass (S/B) ratio and catalyst also played a critical role in tar reduction. This paper demonstrates the conversion of some tar components and elucidates their chemical properties during gasification. | Shuai GUO Xiao WEI Deyong CHE Hongpeng LIU Baizhong SUN | 2021 | Frontiers in Energy2021，15，2： | 0 |
| 15 | Bacterial diversity in Arctic marine sediment determined by culture-dependent and-independent approaches显示文摘Bacterial diversity in surface sediment from the Arctic Ocean was investigated by culture-dependent and-independent approaches. Conventional culture-dependent techniques revealed 11 strains based on their distinct morphological characteristics on marine Zobell 2216E agar plates. Phylogenetic analysis showed that these isolates belonged to three major lineages of the Bacteria, γ-proteobacteria, Bacteroidetes and Actinobacteria, and that they included 10 genera. Most isolates were psychrotrophic, and NaCl was not necessary for their growth. Furthermore, they exhibited activity of at least one extracellular hydrolytic enzyme at 4°C and had various abilities to assimilate carbon sources. A total of 67 phylotypes were detected among 142 clones based on the 16S rRNA library of the total community DNA and grouped into nine major lineages of bacteria. Phylotypes affiliated with γ-, δ- and ε-proteobacteria accounted for 36.7%, 21.8% and 16.9% of the total clones, respectively. The rest of the clones belonged to Bacteroidetes, α-proteobacteria, Actinobacteria, Fusobacteria, Nitrospirae and an unclassified group. | LIN Xuezheng WANG Zhen CHE Shuai SONG Weizhi YI Dan | 2014 | Advances in Polar Science2014，25，1： | 0 |
| 16 | Single-nucleus transcriptomics reveals a gatekeeper role for FoxP1 in primate cardiac aging显示文摘Aging poses a major risk factor for cardiovascular diseases,the leading cause of death in the aged population.However,the cell type-specific changes underlying cardiac aging are far from being clear.Here,we performed single-nucleus RNA-sequencing analysis of left ventricles from young and aged cynomolgus monkeys to define cell composition changes and transcriptomic alterations across different cell types associated with age.We found that aged cardiomyocytes underwent a dramatic loss in cell numbers and profound fluctuations in transcriptional profles.Via transcription regulatory network analysis,we identified FOxP1,a core transcription factor in organ development,as a key downregulated factor in aged cardiomyocytes,concomitant with the dysregulation of FoxP1 target genes associated with heart function and cardiac diseases.Consistently,the deficiency of FOxP1 led to hypertrophic and senescent phenotypes in human embryonic stem cell-derived cardiomyocytes.Altogether,our findings depict the celiular and molecular landscape of ventricular aging at the single-cell resolution,and identify drivers for primate cardiac aging and potential targets for intervention against cardiac aging and associated diseases. | Yiyuan Zhang Yandong Zheng Si Wang Yanling Fan Yanxia Ye Yaobin Jing Zunpeng Liu Shanshan Yang Muzhao Xiong Kuan Yang Jinghao Hu Shanshan Che Qun Chu Moshi Song Guang-Hui Liu Weiqi Zhang Shuai Ma Jing Qu | 2023 | Protein & Cell2023，14，4： | 0 |
| 17 | Discrepancy in photosynthetic responses of the red alga Pyropia yezoensis to dehydration stresses under exposure to desiccation,high salinity,and high mannitol concentration显示文摘Macroalgae that inhabit intertidal zones are exposed to the air for several hours during low tide and must endure desiccation and high variations in temperature, light intensity, and salinity. Pyropia yezoensis (Rhodophyta, Bangiales), a typical intertidal red macroalga that is commercially cultivated in the northwestern Pacific Ocean, was investigated under different dehydration stresses of desiccation, high salinity, and high mannitol concentration. Using chlorophyll fluorescence imaging, photosynthetic activities of P. yezoensis thalli were analyzed using six parameters derived from quenching curves and rapid light curves. A distinct discrepancy was revealed in photosynthetic responses to different dehydration stresses. Dehydration caused by exposure to air resulted in rapid decreases in photosynthetic activities, which were always lower than two other stresses at the same water loss (WL) level. High salinity only reduced photosynthesis significantly at its maximum WL of 40% but maintained a relatively stable maximum quantum yield of photosystem II (PSII) (Fv/Fm). High mannitol concentration induced maximum WL of 20% for a longer time (60 min) than the other two treatments and caused no adverse influences on the six parameters at different WL except for a significant decrease in non-photochemical quenching (NPQ) at 20% WL. Illustrated by chlorophyll fluorescence images, severe spatial heterogeneities were induced by desiccation with lower values in the upper parts than the middle or basal parts of the thalli. The NPQ and rETRmax (maximum relative electron transport rate) demonstrated clear distinctions for evaluating photosynthetic responses, indicating their sensitivity and applicability. The findings of this study indicated that the natural dehydration of exposure to air results in stronger and more heterogeneous effects than those of high salinity or high mannitol concentration. | Guoying Du Xiaojiao Li Junhao Wang Shuai Che Xuefeng Zhong Yunxiang Mao | 2022 | Marine Life Science & Technology2022，4，1： | 0 |
| 18 | The amino acids differences in epitopes may promote the different allergenicity of ovomucoid derived from hen eggs and quail eggs显示文摘Quail egg ovomucoid can inhibit activation of basophils and eosinophils,while hen egg ovomucoid has been shown to be a major allergen,named Gal d 1.At present,the differences in structure and function between two ovomucoid are unclear.We found the homology of ovomucoid in quail eggs and hen eggs reached77%.Compared with hen egg ovomucoid,the distribution of secondary structure was different in AA52-53,AA57-58,AA66-68,AA71-72,AA131-133,AA139-140,AA157-159 and AA184-185.Among 9 epitopes of egg ovomucoid,there were different amino acids from quail egg ovomucoid in 8 epitopes.Recombination quail egg ovomucoid had trypsin inhibition activity and quail egg ovomucoid didn't specifically bind to serum of eggs allergic patients.Quail egg ovomucoid can significantly inhibit RBL-2 H3 cells degranulation and protect cells morphology to a certain extent,indicating quail egg ovomucoid can inhibit cells activation and have potential anti-allergic effects,which is related to trypsin inhibitory activity.The difference in sensitization compare to hen egg ovomucoid may be due to amino acids differences affecting protein structure by changing antigenic epitopes. | Mengzhen Hao Shuai Yang Shiwen Han Huilian Che | 2023 | Food Science and Human Wellness2023，12，3： | 0 |
| 19 | Spin Polarizations in a Covariant Angular Momentum Conserved Chiral Transport Model显示文摘To achieve total angular momentum conservation is highly nontrivial in constructing transport models when the spin-orbit interactions are not negligible.In our recent works[1,2],we have developed the first transport model that rigorously respects this fundamental conservation law by carefully including the side-jump effect[3,4]caused by spin-orbit interactions. | Liu Shuai Y.F. Sun Yifeng Che Ming Ko | 2019 | IMP & HIRFL Annual Report2019，，1： | 0 |
| 20 | Coupling Effects of A Deep-Water Drilling Riser and the Platform and the Discharging Fluid Column in An Emergency Disconnect Scenario显示文摘As drilling operations move into remote locations and extreme water depths, recoil analysis requires more careful considerations and the incidence of emergency disconnect is increased inevitably. To accurately capture the recoil dynamics of a deep-water riser in an emergency disconnect scenario, researchers typically focus on modelling the influential subsystems (e.g., the tensioner, the mud discharge and seawater refilling process) which can be solved in the preprocessing, and then the determined parameters are transmitted into an existing global riser analysis software. Distinctively, the current study devotes efforts into the coupling effects resulting from that the suspended riser reacts the platform heave motion via the tensioner system in the course of recoil and the discharging fluid column follows the oscillation of the riser in the mud discharge process. Four simulation models are established based on lumped mass method employing different formulas for the top boundary condition of the riser and the discharging flow acceleration. It demonstrates that the coupling effects discussed above can significantly affect the recoil behavior during the transition phase from initial disconnect to the final hang-off state. It is recommended to develop a fully- coupled integrated model for recoil analysis and anti-recoil control system design before extreme deep-water applications. | MENG Shuai CHEN Yong CHE Chi-dong | 2020 | China Ocean Engineering2020，34，1： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员