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| 1 | Suppression of P-gp induced multiple drug resistance in a drug resistant gastric cancer cell line by overexpression of Fas显示文摘AIM To observe the drug sensitizing effect andrelated mechanisms of fas gene transduction onhuman drug-resistant gastric cancer cellSGC7901/VCR(resistant to Vincristine).METHODS The cell cycle alteration wasobserved by FACS.The sensitivity of gastriccancer cells to apoptosis was determined by invitro apoptosis assay.The drug sensitization ofcells to several anti-tumor drugs was observedby MTT assay.Immunochemical method wasused to show expression of P-gp and Topo Ⅱ ingastric cancer cells.RESULTS Comparing to SGC7901 and pBK-SGC7901/VCR,fas-SGC7901/VCR showeddecreasing G2 cells and increasing S cells,theG2 phase fraction of pBK-SGC7901/VCR wasabout 3.0 times that of fas-SGC7901/VCR,but Sphase fraction of fas-SGC7901/VCR was about1.9 times that of pBK-SGC7901/VCR,indicatingS phase arrest of fas-SGC7901/VCR.FACS alsosuggested apoptosis of fas-SGC7901/VCR,fas-SGC7901/VCR was more sensitive to apoptosisinducing agent VM-26 than pBK-SGC7901/VCR.MTT assay showed increased sensitization offas-SGC7901/VCR to DDP,MMC and 5-FU,butsame sensitization to VCR according to pBK-SGC7901/VCR.SGC7901,pBK-SGC7901/ VCRand fas-SGC7901/VCR had positively stainedTopo Ⅱ equally.P-gp staining in pBK- SGC7901/VCR was stronger than in SG07901,but there was little staining of P-gp in fas.SGC7901/VCR.CONCLUSION fas gene transduction couldreverse the MDR of human drug-resistant gastriccancer cell SGC7901/VCR to a degree,possiblybecause of higher sensitization to apoptosis anddecreased expression of P-gp. | Yin F Shi YQ Zhao WP Xiao B Miao JY Fan DM | 2000 | World Journal of Gastroenterology2000，6，5： | 24 |
| 2 | Down-regulation of Hsp90 could change cell cycle distribution and increase drug sensitivity of tumor cells显示文摘:AIM To construct Hsp90 antisense RNAeukaryotic expression vector, transfect it intoSGC7901 and SGC7901/VCR of MDR-type humangastric cancer cell lines, HCC7402 of humanhepatic cancer and Eel09 of human esophagealcancer cell lines, and to study the cell cycledistribution of the gene transected cells andtheir response to chemotherapeutic drugs.METHODS A I .03kb cDNA sequence of Hsp90Pwas obtained from the primary plasmid phHsp90by EcoR 1 and BamH I nuclease digestion andwas cloned to the EcoR 1 and BamH 1 site ofthe pcDNA by T4DNA ligase and an antisenseorientation of Hsp900 expression vector wasconstructed. The constructs were transfectedwith lipofectamine and positive clones wereselected with G418. The expression of RNA wasdetermined with dot blotting and RNaseprotection assay, and the expression of Hsp90protein determined with Western blot. Cell cycledistribution of the transfectants was analyzedwith flow cytometry, and the drug sensitivity ofthe transfectants to adriamycin (ADR ),vincrinstine (VCR ), mitomycin (MMC ) andcyclophosphamide (CTX ) with MTT andintracellular drug concentration of thetransfectants was determined with flowcytometry.RESULTS In EcoR 1 and BamH I restrictionanalysis, the size and the direction of the clonedsequence of Hsp900 remained what had beendesigned and the gene constructs were namedpcDNA-Hsp90. AH^SGC7901, AH^SGC7901/ VCR,AH-HCC7402 and AH-Eel09 cell clones allexpressed Hsp90 anti--sense RNA. Theexpression of Hsp90 was down--regulated in AHSGC7901, AH--SGC7901/ VCR, AH-HCC7402 andAH--Eel09 cell clones. Cell cycle distribution waschanged differently. In AH-SGC7901/ VCR andAH-Ec109 cells, G, phase cells were increased; Sphase and G, phase cells were decreased ascompared with their parental cell lines. In AHSGC7901 cell, G, phase cells were decreased, Qphase cells increased and S phase cells were notchanged, and in AH-HCC7402 cells G,, S and qphase cells remained unchanged as comparedwith their parental cell lines. The sensitivity ofAH--SGC7901, AH--SGC7901/ VCR, AH-HCC7402 andAH-Ec109 to chemotherapeutic drugs, thesensitivity ot AH--SGC7901/ VCR to ADR, VCR,MMC and CTX the sensitivity of AH-HCC7402 toADR and VCR, and the sensitivity of Eel09 toADR, VCR and CTX all increased as comparedwith their parental cell lines. The meanfluorescence intensity of ADR in AH--SGC7901,AH-SGC7901/ VCR, AH--HCC7402 and AH-Ec109was also significantly elevated (P< 0. 05).CONCLUSION Down-regulation of HsP90 couldchange cell cycle distribution and increase thedrug sensitivity of tumor cells. | Liu XL Xiao B Yu ZC Guo JC Zhao QC Xu L Shi YQ Fan DM | 1999 | World Journal of Gastroenterology1999，5，3： | 21 |
| 3 | Anatomic structural study of cerebellopontine angle via endoscope显示文摘在颅骨底依靠寻找内视镜的技术的可能的解剖基础的背景最低限度地侵略的外科是争论的。这研究的目的是在 cerebellopontine 角度区域观察在主要的血容器和神经之间的空间关系并且为侧面、以后的颅骨底提供解剖基础经由内视镜的 retrosigmoid 锁眼 approach.Methods 的最低限度地侵略的外科这研究在三十个弄干的成年颅骨上被进行测量空间关系在之中出现以后的头部的窝的多骨的标记,并且为 retrosigmoid 锁眼 ap 定位最适当的钻区域在直径与 2.0 厘米在这个点上集中的一个洞对在 cerebellopontine 角度暴露相关结构合适。 Retrosigmoid 锁眼途径罐头减少小脑上的压力并且有效地暴露相关结构它包括面部神经, vestibulocochlear 神经,三叉的神经,舌与喉的神经,迷走神经神经,附件神经,舌下神经神经,前面的劣等的小脑的动脉,以后的劣等的小脑的动脉和迷宫的动脉,内诊镜 retrosigmoid 途径上的准确地点能避免在最低限度地侵略的外科期间拖小脑的 etc.Conclusions 。retrosigmoid 锁眼途径的申请将扩大内视镜的技术的申请。 | XIA Yin LI Xi-ping HAN De-min ZHENG Jun LONG Hai-shan SHI Jin-feng Department of Otolaryngology,Beijing Tongren Hospital,Capital Medical University Key Laboratory of Otolaryngology Head and Neck Surgery (Capital Medical University),Ministry of Education,Beijing 100730,China (Xia Y,Li XP,Han DM,Zheng J,Long HS and Shi JF) | 2007 | Chinese Medical Journal2007，，20： | 7 |
| 4 | Biomechanical comparison of different pedicle screw fixations for thoracolumbar burst fractures using finite element method显示文摘 | Wang DM Shi DF Li XL | 2012 | Appl Mater2012，117，9： | 1 |
| 5 | Minimally invasive versus stemotomy approach for mitral valve surgery in patients greater than 70 years old: a propensity-matched comparison 显示文摘 | Holzhey DM Shi W Borger MA | 2011 | Ann Thorac Surg2011，91，2： | 1 |
| 6 | Landscape analysis of dynamic soil erosion in Subtropical China: A case study in Xingguo County, Jiangxi Province显示文摘 | Wang K Wang H J Shi XZ Weindorf DC Yu DS Liang Y Shi DM | 2009 | Soil and Tillage Research2009，109，： | 1 |
| 7 | Relationship between soil erosion and distance to roadways in undeveloped areas of China显示文摘 | Shi XZ Wang K Warner ED Yu DS Wang HJ Yang RW Liang Y Shi DM | 2008 | Catena2008，72，： | 1 |
| 8 | α1-Adrenergic receptor stimulates IL-6 expression and secretion through both mRNA stability and transcriptional regulation: involvement of p38 MAPK and NF-κB 显示文摘 | Perez DM Papay RS Shi T | 2009 | Mole Pharmacol2009，76，1： | 1 |
| 9 | Relationship between soil erosion and distance to roadways in undeveloped areas of China显示文摘 | Shi XZ Wang K Warner ED Yu DS Wang HJ Yang RW Liang Y Shi DM | 2008 | Catena2008，72，： | 1 |
| 10 | Tumor suppressor XAF1 induces ap-optosis ,inhibits angiogenesis and inhibits tumor growth in hepatocellu-lar carcinoma 显示文摘 | Zhu LM Shi DM Dai Q | 2014 | Oncotarget2014，5，14： | 1 |
| 11 | Effect of heating and exposure to light on the stability of lycopene in tomato puree 显示文摘 | SHI J DM Y Z KAKUDA Y | 2008 | Food Control2008，19，： | 1 |
| 12 | Lithium reduces ischemia- induced hippocampal CA1 damage and behavioral de? cits in gerbils显示文摘 | Bian Q Shi T Chuang DM | 2007 | Brain Res2007，1184，12： | 1 |
| 13 | Association among retinol-binding protein 4, small dense LDL cholesterol and oxidized LDL levels in dyslipidemia subjects显示文摘 | Wu J Shi YH Niu DM | 2012 | Clin Biochem2012，45，9： | 1 |
| 14 | Insulin receptorsubstrate-1 suppresses transforming growth factor-beta1-mediated epithelial-mesenchymal transition显示文摘 | Shi J Wang DM Wang CM | 2009 | CancerRes2009，69，18： | 1 |
| 15 | Insulin receptor substrate-1 suppresses transforming growth factor- betal-mediated epithelial-mesenchymal transition 显示文摘 | Shi J Wang DM Wang CM | 2009 | Cancer Res2009，69，18： | 1 |
| 16 | NF90 regulates cell cycle exit and terminal myogenic differentiation by direct binding to the 3-untranslated region of MyoD and p21^WAF1/CIP1 mRNAs 显示文摘 | Shi LF Zhao GH Qiu DM | 2005 | J Biol Chem2005，280，18： | 1 |
| 17 | Minimally invasive versus sternotomy approach for mitral valve surgery in patients greater than 70 years old:a propensity-matched comparison显示文摘 | Holzhey DM Shi W Borger MA | 2011 | Ann Thorac Surg2011，91，2： | 1 |
| 18 | The leaf size-twig size spectrum of temperate woody species along an altitudinal gradient:an invariant allometric scaling relationship显示文摘 | Sun SC Jin DM Shi PL | | 0，，： | 1 |
| 19 | α1-Adrenergic receptor stimulates IL-6expression and secretion through both mRNA stability and transcriptional regulation:involvement of p38 MAPK and NF-KB显示文摘 | Perez DM Papay RS Shi T | 2009 | Mol Pharmacol2009，76，： | 1 |
| 20 | Paradoxical reduction of fatty streak formation in mice lacking endothelial nitric oxide synthase 显示文摘 | Shi W Wang X Shih DM | 2002 | Circulation2002，105，17： | 1 |
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