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| 1 | HLA-A Gene Polymorphism Defined by High-Resolution Sequence-Based Typing in 161 Northern Chinese Han People显示文摘Human leukocyte antigen(HLA)system is the most polymorphic region known in the human genome.In the present study,we analyzed for the first time the HLA-A gene polymorphisms defined by the high-resolution typing methods-sequence-based typing(SBT)in 161 Northern Chinese Han people. A total of 74 different HLA-A gene types and 36 alleles were detected. The most frequent alleles were A 110101(GF=0.2360),A 24020101(GF=0.1646),and A 020101(GF=0.1553);followed by A 3303(GF=0.1180),A3001(GF=0.0590),and A310102(GF=0.0404).The frequencies of following alleles,A0203,A0205,A0206,A0207,A030101,A2423,A2601,A3201,and A3301,are all higher than 0.0093. The homozygous alleles include A020101,A110101,A24020101 and A310102.Heterozygosity(H),polymorphism information content (PIC),discrimination power(DP)and probability of paternity exclusion(PPE)of HLA-A in the samples were calculated and their values were 0.8705,0.8491,0.6014,and 0.9475,respectively. Han population,especially the allele subtypes character,will be of great interest for clinical transplantation, disease-associated study and forensic identification. Implementation of high-resolution typing methods allows a significantly wider spectrum of HLA variation including rare alleles. This rpectrum will further be extensively utilized in many fields. | ChunxiaYan RuilinWang JingxiangLi YajunDeng DongyingWu HongboZhang HongxingZhang LidongWang ChunrongZhang HaiyanSun XiuqingZhang JianWang HuanmingYang ShengbinLi | 2003 | Genomics, Proteomics & Bioinformatics2003，1，4： | 13 |
| 2 | The Structural Characterization and Antigenicity of the S Protein of SARS-CoV显示文摘The corona-like spikes or peplomers on the surface of the virion under electronicmicroscope are the most striking features of coronaviruses. The S (spike) proteinis the largest structural protein, with 1,255 amino acids, in the viral genome. Itsstructure can be divided into three regions: a long N-terminal region in the exte-rior, a characteristic transmembrane (TM) region, and a short C-terminus in theinterior of a virion. We detected fifteen substitutions of nucleotides by comparisonswith the seventeen published SARS-CoV genome sequences, eight (53.3%) of whichare non-synonymous mutations leading to amino acid alternations with predictedphysiochemical changes. The possible antigenic determinants of the S protein arepredicted, and the result is confirmed by ELISA (enzyme-linked immunosorbentassay) with synthesized peptides. Another profound finding is that three disulfidebonds are defined at the C-terminus with the N-terminus of the E (envelope) pro-tein, based on the typical sequence and positions, thus establishing the structuralconnection with these two important structural proteins, if confirmed. Phyloge-netic analysis reveals several conserved regions that might be potent drug targets. | JingxiangLi ChunqingLuo YajunDeng YujunHan LingTang JingWang JiaJi JiaYe FanboJiang ZhaoXu WeiTong WeiWei QingrunZhang ShengbinLi WeiLi HongyanLi YudongLi WeiDong JianWang ShengliBi HuanmingYaug | 2003 | Genomics, Proteomics & Bioinformatics2003，1，2： | 5 |
| 3 | Gene Expression Profiling in Porcine Fetal Thymus显示文摘obtain an initial overview of gene diversity and expression pattern in porcinethymus, 11,712 ESTs (Expressed Sequence Tags) from 100-day-old porcine thymus(FTY) were sequenced and 7,071 cleaned ESTs were used for gene expressionanalysis. Clustered by the PHRAP program, 959 contigs and 3,074 singlets wereobtained. Blast search showed that 806 contigs and 1,669 singlets (totally 5,442ESTs) had homologues in GenBank and 1,629 ESTs were novel. According to theGene Ontology classification, 36.99% ESTs were cataloged into the gene expressiongroup, indicating that although the functional gene (18.78% in defense group) ofthymus is expressed in a certain degree, the 100-day-old porcine thymus still existsin a developmental stage. Comparative analysis showed that the gene expressionpattern of the 100-day-old porcine thymus is similar to that of the human infantthymus. | YanjiongChen ShengbinLi LinYe JianingGeng YajunDeng SongnianHu | 2003 | Genomics, Proteomics & Bioinformatics2003，1，2： | 1 |
| 4 | Polymorphism Profile of Nine Short Tandem Repeat Loci in the Han Chinese显示文摘Nine short tandem repeat (STR) markers (D3S1358, VWA, FGA, THO1, TPOX,CSFIPO, D5S818, D13S317, and D7S820) and a sex-identification marker (Amel-ogenin locus) were amplified with multiplex PCR and were genotyped with afour-color fluorescence method in samples from 174 unrelated Han individuals inNorth China. The allele frequencies, genotype frequencies, heterozygosity, prob-ability of discrimination powers, probability of paternity exclusion and Hardy-Weinberg equilibrium expectations were determined. The results demonstratedthat the genotypes at all these STR loci in Han population conform to Hardy-Weinberg equilibrium expectations. The combined discrimination power (DP) was1.05 × 10-10 within nine STR loci analyzed and the probability of paternity exclusion(EPP) was 0.9998. The results indicate that these nine STR loci and the Amelo-genin locus are useful markers for human identification, paternity and maternitytesting and sex determination in forensic sciences. | ShuangdingLi ChunxiaYan YajunDeng RuilinWang JianWang HuanmingYan ShengbinLi | 2003 | Genomics, Proteomics & Bioinformatics2003，1，2： | 0 |
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