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| 1 | Increased expression of tumor necrosis factor-a is associated with advanced colorectal cancer stages显示文摘AIM:To detect the expression of tumor necrosis factor-a(TNF-a)in colorectal cancer(CRC)cells among Saudi patients,and correlate its expression with clinical stages of cancer.METHODS:Archival tissue specimens were collected from 30 patients with CRC who had undergone surgical intervention at King Khalid University Hospital.Patient demographic information,including age and gender,tumor sites,and histological type of CRC,was recorded.To measure TNF-a m RNA expression in CRC,total RNA was extracted from tumor formalin-fixed,paraffinembedded,and adjacent normal tissues.Reverse transcription and reverse transcription polymerase chain reaction were performed.Colorectal tissue microarrays were constructed to investigate the protein expression of TNF-a by immunohistochemistry.RESULTS:The relative expression of TNF-a m RNA in colorectal cancer was significantly higher than that seen in adjacent normal colorectal tissue.High TNF-a gene expression was associated with StageⅢandⅣneoplasms when compared with earlier tumor stages(P=0.004).Eighty-three percent of patients(25/30)showed strong TNF-a positive staining,while only 10%(n=3/30)of patients showed weak staining,and 7%(n=2/30)were negative.We showed the presence of elevated TNF-a gene expression in cancer cells,which strongly correlated with advanced stages of tumor.CONCLUSION:High levels of TNF-a expression could be an independent diagnostic indicator of colorectal cancer,and targeting TNF-a might be a promising prognostic tool by assessment of the clinical stages of CRC. | Omar A Al Obeed Khayal A Alkhayal Abdulmalik Al Sheikh Ahmad M Zubaidi Mansoor-Ali Vaali-Mohammed Robin Boushey James H Mckerrow Maha-Hamadien Abdulla | 2014 | World Journal of Gastroenterology2014，20，48： | 8 |
| 2 | Urinary nuclear magnetic resonance spectroscopy of a Bangladeshi cohort with hepatitis-B hepatocellular carcinoma: A biomarker corroboration study显示文摘AIM: To establish if a distinct urinary metabolic profile could be identified in Bangladeshi hepatitis-B hepatocellular carcinoma(HCC) patients compared to cirrhosis patients and controls. METHODS: Urine samples from 42 Bangladeshi patients with HCC(39 patients with hepatitis-B HCC), 47 with cirrhosis on a background of hepatitis B, 46 with chronic hepatitis B, and seven ethnically-matched healthy controls were analyzed using nuclear magnetic resonance(NMR) spectroscopy. A full dietary and medication history was recorded for each subject. The urinary NMR data were analyzed using principal component analysis(PCA) and orthogonal partial leastsquared discriminant analysis(OPLS-DA) techniques. Differences in relative signal levels of the most discriminatory metabolites identified by PCA and OPLSDA were compared between subject groups using an independent samples Kruskal-Wallis one-way analysis of variance(ANOVA) test with all pairwise multiple comparisons. Within the patient subgroups, the MannWhitney U test was used to compare metabolite levels depending on hepatitis B e-antigen(HBe Ag) status and treatment with anti-viral therapy. A BenjaminiHochberg adjustment was applied to acquire the level of significance for multiple testing, with a declared level of statistical significance of P < 0.05.RESULTS: There were significant differences in age(P < 0.001), weight(P < 0.001), and body mass index(P < 0.001) across the four clinical subgroups. Serum alanine aminotransferase(ALT) was significantly higher in the HCC group compared to controls(P < 0.001); serum α-fetoprotein was generally markedly elevated in HCC compared to controls; and serum creatinine levels were significantly reduced in the HCC group compared to the cirrhosis group(P = 0.004). A threefactor PCA scores plot showed clustering of the urinary NMR spectra from the four subgroups. Metabolites that contributed to the discrimination between the subgroups included acetate, creatine, creatinine, dimethyamine(DMA), formate, glycine, hippurate, and trimethylamine-N-oxide(TMAO). A comparison of relative metabolite levels confirmed that carnitine was significantly increased in HCC; and creatinine, hippurate, and TMAO were significantly reduced in HCC compared to the other subgroups. HBe Ag negative patients showed a significant increase in creatinine(P = 0.001) compared to HBe Ag positive patients in the chronic hepatitis B subgroup, whilst HBe Ag negative patients showed a significant decrease in DMA(P = 0.004) in the cirrhosis subgroup compared to HBe Ag positive patients. There were no differences in metabolite levels in HCC patients who did or did not receive antiviral treatment. CONCLUSION: Urinary NMR changes in Bangladeshi HCC were identified, corroborating previous findings from Egypt and West Africa. These findings could form the basis for the development of a cost-effective HCC dipstick screening test. | I Jane Cox Abil E Aliev Mary ME Crossey Mahvish Dawood Mamun Al-Mahtab Sheikh M Akbar Salimur Rahman Antonio Riva Roger Williams Simon D Taylor-Robinson | 2016 | World Journal of Gastroenterology2016，22，16： | 4 |
| 3 | Effect of hyperoxia on vascular endothelial growth factor levels in a wound model显示文摘 | Sheikh A Y Gibson J J Rollins M D | 2000 | Arch Surg2000，135，11： | 1 |
| 4 | Utility of vol- ume adjusted prostate specific antigendensity in the diagnosis of prostate cancer in Arab men显示文摘 | SHEIKH M AL SAEED O KEHINDE E O | 2005 | Int Urol Nephrol2005，37，4： | 1 |
| 5 | Percutaneous coblation nucleoplasty in patients with contained lumbar disc pro- lapse: 1 year follow-up in a prospective case series 显示文摘 | Sinan T Sheikh M Burie J | 2011 | Acta Neurochir Suppl2011，108，： | 1 |
| 6 | Connexin43deficiency causes delayed ossification, craniofacial abnormalities,and osteoblast dysfunction显示文摘 | LECANDA F WARLOW P M SHEIKH S | 2000 | J Cell Biol2000，151，4： | 1 |
| 7 | Analytical model for concrete confinement in tied columns 显示文摘 | Sheikh S A Uzumeri S M | 1982 | Journal of the Structural Division ASCE1982，108，12： | 1 |
| 8 | Effica- cy of dexmedetomidine in suppressing cardiovascular and hormonal responses to general anaesthesia for caesarean delivery: a dose -re- sponse study显示文摘 | EL TAHAN M R MOWAFI H A AL SHEIKH L H | 2012 | Int J Obstet Anesth2012，21，3： | 1 |
| 9 | Analytical model for concrete confine- ment in tied columns显示文摘 | Sheikh S A Uzumeri S M | 1982 | Journal of the Structural Division1982，108，12： | 1 |
| 10 | The development of live load for long span bridges显示文摘 | Nowak A S Lutomirska M Sheikh Ibrahim F I | 2010 | Bridge Structures-Assessment Design&Construction2010，6，1： | 1 |
| 11 | Role of ultrasonography in blunt abdominal trauma,a prospective study显示文摘 | Al-Zayat I Sheikh M Mousa I Behbe-hani A | 1996 | Eur J Surg1996，162，： | 1 |
| 12 | Bile acid signaling in fetal tissues:implications for intrahepatic cholestasis of pregnancy显示文摘 | Williamson C Miragoli M Sheikh AKS | | 0，，01： | 1 |
| 13 | A Reliability Model For Fatigue Life Characterization显示文摘 | | 1995 | International Journal of Fatigue1995，17，2： | 1 |
| 14 | Investigation of Heat Partition in High Speed Turning of High Strength Alloy Steel显示文摘 | Abukhshim N A Mativenga P T Sheikh M A | 2005 | International Journal of Machine Tools and Manufacture2005，45，15： | 1 |
| 15 | Deep-seated Thoracic and Abdominal Masses:Usefulness of Ultrasound and Computed Tomography Guidance in Fine Needle Aspiration Cytology Diagnosis显示文摘 | Sheikh M Sawhney S Dey P | 2000 | Australas Radiol2000，44，2： | 1 |
| 16 | Spinal dural arteriovenous fistu-la mimicking prostate hyperplasia显示文摘 | Sheikh SI Busl KM Ning M | 2011 | J Emerg Med2011，41，6： | 1 |
| 17 | Retinoic acid and estrogen modulation of insulin-like growth factor binding protein-4 gene expression and the estrogen receptor status of human breast carcinoma cells显示文摘 | SHEIKH M S SHAO Z M HUSSAIN A | 1993 | Biochemical and Biophysical Research Communications1993，193，3： | 1 |
| 18 | A probabilistic approach to the maintenance of heat-transfer equipment subject to fouling显示文摘 | Zubair S M Sheikh A K Shaik M N | 1992 | Energy1992，17，8： | 1 |
| 19 | Analytical model for concrete confinement in tied columns 显示文摘 | Sheikh S A Uzumeri S M | 1982 | J Structural Division ASCE1982，108，12： | 1 |
| 20 | Beam-column moment connections for composite frames:part 1显示文摘 | Sheikh T M Deierlein G G Yura J A | 1989 | Journal of Structural Engineering1989，115，11： | 1 |
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