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| 1 | Mining,characterization,and exploitation of EST-derived microsatellites in Gossypium barbadense显示文摘Simple sequence repeats(SSRs) have been widely applied as molecular markers in genetic studies.However,the number of expressed sequence tags(ESTs) and SSR markers from Gossypium barbadense is fewer than those from other cotton species.In this study,EST-SSR distribution from G.barbadense was characterized and new G.barbadense-derived EST-SSR markers were determined on the basis of the ESTs obtained by randomly sequencing 2 cDNA libraries associated with fiber development in G.barbadense.By mining 9697 non-redundant ESTs,a total of 638 SSR loci derived from 595 ESTs were observed.In G.barbadense,the frequency of ESTs containing SSRs was 6.13%,with an average of 1 SSR in every 10.4 kb of EST sequence.Furthermore,trinucleotide was found to be the most abundant repeat type among 2-6-nucleotide repeat types.It accounted for 26.6% of the total,followed by the hexanucleotide(26.0%) and pentanucleotide repeats(25.9%).Among all the repeat motifs,(AAG)n accounted for the highest proportion.EST-SSR primer pairs were developed using the Primer3 program,and the redundant primers were removed using the virtual PCR approach.As a result,380 non-redundant EST-SSR primer pairs were developed and used to detect polymorphisms between the mapping parents G.hirsutum 'TM-1' and G.barbadense 'Hai7124' for constructing linkage groups in cultivated allotetraploid cotton.Out of these,98(25.8%) primer pairs detected polymorphisms.Finally,95 polymorphic loci from 82 primer pairs were integrated into the backbone genetic map;of these,42 were mapped into the A subgenome and 53 into the D subgenome.The present work provided the foundation for constructing saturated genetic maps and conducting comparative genomic studies on different cotton species. | LU YuanDa CAI CaiPing WANG Lei LIN ShaoYan ZHAO Liang TIAN LiangLiang LU JunHong ZHANG TianZhen GUO WangZhen | 2010 | Chinese Science Bulletin2010，55，18： | 5 |
| 2 | Coronary heart disease: incidence, risk factors and interventions in Jiaozhou of Shandong province显示文摘 | Yu Hua Li Dan Chu Xianming An Yi Song Tongxun Feng Huixin Lin Peilin Wang Tao Jiang Shaoyan Guo Linlin Xu Fengqiang Liu Zhengke Yang Bin | 2014 | Chinese Medical Journal2014，，12： | 5 |
| 3 | Polymorphisms of MTHFR and TYMS predict capecitabine-induced hand-foot syndrome in patients with metastatic breast cancer显示文摘Background:Breast cancer is a global problem,and a large number of new cases are diagnosed every year.Capecit-abine is effective in patients with metastatic breast cancer(MBC).Hand-foot syndrome(HFS)is a common adverse effect of capecitabine.In this study,we investigated the association between single nucleotide polymorphisms(SNPs)in genes involved in capecitabine metabolism pathways and capecitabine-induced HFS in Chinese patients with MBC to identify some predictive genetic biomarkers.Methods:We selected 3 genes involved in capecitabine metabolism and screened genetic variants in these target genes.We genotyped a total of 22 SNPs in the thymidylate synthase gene(TYMS),the methylene tetrahydrofolate reductase gene(MTHFR),and the ribonucleotide reductase M1 gene(RRM1)in 342 MBC patients treated with capecit-abine-based chemotherapy.The genotype distributions of each SNP in patients with and without HFS were assessed using Pearson’sχ^(2)test,and the relationship between HFS and genotypes of SNPs was determined using logistic regression analysis.The association between SNPs and their corresponding gene expression was analyzed using the Blood expression quantitative trait loci(eQTL)browser online tools.Results:We found 4 positive sites for HFS in the TYMS and MTHFR genes:TYMS rs2606241(P=0.022),TYMS rs2853741(P=0.019),MTHFR rs3737964(P=0.029),and MTHFR rs4846048(P=0.030).Logistic regression analyses showed that the genotype AG of MTHFR rs3737964[odds ratio(OR)=0.54,95%confidence interval(CI)0.31-0.97,P=0.038]and MTHFR rs4846048(OR=0.54,95%CI 0.30-0.98,P=0.042)were protective factors of HFS,whereas the genotype CT of TYMS rs2853741(OR=2.25,95%CI 1.31-3.87,P=0.012)increased the risk of HFS.The association between the genotype GT of TYMS rs2606241(OR=1.27,95%CI 0.73-2.23,P=0.012)and HFS was uncertain.Further eQTL analyses confirmed that the alleles of rs3737964 and rs4846048 affected the gene expression levels of MTHFR in cis.Conclusions:We have identified four potentially useful pharmacogenetic markers,TYMS rs2606241,TYMS rs2853741,MTHFR rs3737964,and MTHFR rs4846048 to predict capecitabine-induced HFS in MBC patients. | Shaoyan Lin Jian Yue Xiuwen Guan Peng Yuan Jiayu Wang Yang Luo Ying Fan Ruigang Cai Qiao Li Shanshan Chen Pin Zhang Qing Li Fei Ma Binghe Xu | 2019 | Cancer Communications2019，39，1： | 3 |
| 4 | National guidelines for diagnosis and treatment of thyroid cancer 2022 in China(English version)显示文摘Contents 1. Overview 2. Diagnosis and management 2.1 Screening for high-risk group 2.2 Clinical manifestations 2.2.1 Symptoms 2.2.2 Signs 2.2.3 Invasion and metastasis 2.3 Laboratory examination 2.3.1 Routine examination 2.3.2 Special examination 2.4 Imaging 2.4.1 Ultrasonography 2.4.2 Computed tomography (CT)2.4.3 Magnetic resonance imaging (MRI)2.4.4 Positron emission tomography (PET)-CT。 | 无 Shaoyan Liu Yansong Lin Yiming Zhu Lin Gui Xiaorong Hou Ziren Kong Yuanlin Piao Yuqing Sun Hao Wang Jian Wang Bo Zhang Ye Zhang Kun Zheng | 2022 | Chinese Journal of Cancer Research2022，34，3： | 2 |
| 5 | Comments on National guidelines for diagnosis and treatment of thyroid cancer 2022 in China (English version)显示文摘Thyroid cancer (TC) is the most common endocrine system cancer, of note, the overall survival of TC in China is suboptimal when comparing with the developed countries such as US (84.3%vs.98.3%), posing a great challenge among professionals involved in this field.Standardization of its diagnosis and treatment not only provides the basis for all the care givers to promote the entire level of TC management, but also is helpful in shortening the distance between China and other developed countries. A multidisciplinary team (MDT)should be involved in the comprehensive clinical management of TC, particularly for those advanced or refractory TC, which needs cooperation among members from ultrasonography, radiology, pathology, surgery. | Hao Wang Jian Wang Xiang Wang Yiming Zhu Yuqing Sun Xin Zhang Xiaorong Hou Kun Zheng Shaoyan Liu Yansong Lin | 2022 | Chinese Journal of Cancer Research2022，34，5： | 2 |
| 6 | Experimental investigation on the dielectrlc-behavior of nanostructured rutilephase titania显示文摘 | Ye Xisheng Xiao Zhigang Lin Dongsheng Huang Shaoyan Man Yihang | 2000 | Mater Sci and Eng B2000，74，： | 1 |
| 7 | MTHFR和TYMS的多态性预测转移性乳腺癌患者因卡培他滨诱发的手足综合征显示文摘背景与目的乳腺癌是一个全球性问题,每年都会诊断出大量的新发病例。卡培他滨对转移性乳腺癌(metastatic breast cancer,MBC)患者有效。手足综合征(hand-foot syndrome,HFS)是卡培他滨的常见不良反应。在本文中,我们研究了卡培他滨代谢通路相关基因中的单核苷酸多态性(single nucleotide polymorphisms,SNPs)与卡培他滨诱导的中国MBC患者HFS之间的相关性,以确定一些具有预测性的生物标志物基因。方法我们选择了3个参与卡培他滨代谢的基因,并筛选了这些靶基因的遗传变体。我们对342例接受卡培他滨化疗的MBC患者的胸苷酸合酶基因(thymidylate synthase gene,TYMS)、亚甲基四氢叶酸还原酶基因(methylene tetrahydrofolate reductase gene,MTHFR)和核糖核苷酸还原酶M1基因(ribonucleotide reductaseM1gene,RRM1)中的22个SNPs进行了基因分型。使用Pearson’sχ2检验评估患有和不患有HFS的患者中每个SNP的基因型分布,并使用logistic回归分析确定HFS与SNP的基因型之间的相关性。使用血液表达数量定量性状基因座(expression quantitative trait loci,e QTL)Browser在线工具分析了SNP及其对应基因表达之间的相关性。结果我们在TYMS和MTHFR基因中发现了4个HFS阳性位点:TYMS rs2606241(P=0.022)、TYMS rs2853741(P=0.019)、MTHFR rs3737964(P=0.029)和MTHFR rs4846048(P=0.030)。Logistic回归分析显示,MTHFR rs3737964[比值比(odds ratio,OR)=0.54,95%置信区间(confidence interval,CI):0.3–0.97,P=0.038]和MTHFRrs4846048(OR=0.54,95%CI:0.30–0.98,P=0.042)的基因型AG是HFS的保护因素,而TYMS rs2853741的基因型CT(OR=2.25,95%CI:1.31–3.87,P=0.012)增加了HFS的风险。TYMSrs2606241的基因型GT(OR=1.27,95%CI:0.73–2.23,P=0.012)与HFS之间的相关性尚不确定。进一步的e QTL分析证实,rs3737964和rs4846048的等位基因影响顺式MTHFR的基因表达水平。结论我们已经确定了4个潜在有效的药物遗传学标记,TYMS rs2606241、TYMS rs2853741、MTHFR rs3737964和MTHFR rs4846048可以预测卡培他滨诱导的MBC患者的HFS。 | Shaoyan Lin Jian Yue Xiuwen Guan Peng Yuan Jiayu Wang Yang Luo Ying Fan Ruigang Cai Qiao Li Shanshan Chen Pin Zhang Qing Li Fei Ma Binghe Xu | 2019 | 癌症2019，38，11： | 1 |
| 8 | CACA guidelines for holistic integrative management of thyroid cancer显示文摘Purpose:In recent years,thyroid cancer is a common clinical problem.Since guidelines for the diagnosis and treatment of thyroid nodules and diferentiated thyroid cancer were revised in 2012,signifcant scientifc advances have occurred in the feld.The aim of this guidelines is to inform clinicians,researchers,patients and health policy makers on published evidence and expert consensus relating to the diagnosis and management of thyroid malignancy.Methods:In order to better promote the clinical management of thyroid cancer in China,Chinese Association of Thyroid Oncology(CATO)organized relevant experts to write these guidelines based on latest relevant literatures and clinical experience of multiple centers.The specifc clinical issues and topics addressed in these guidelines were based on published evidence,prior versions of the Chinese guidelines and expert consensus.Results and conclusion:The guidelines provide recommendations for the management of diferent types of thyroid carcinoma,including papillary,follicular,medullary,and anaplastic carcinomas. | Minghua Ge Ming Gao Ruochuan Cheng Xiaohong Chen Haixia Guan Yansong Lin Shaoyan Liu Yu Wang Chuanming Zheng Xiangqian Zheng | 2022 | Holistic Integrative Oncology2022，1，1： | 0 |
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