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| 1 | Different Expression Analysis in Fruit Softening and Ethylene Biosynthetic Pathways in Peaches of Different Flesh Textures显示文摘The aim of our study was to assess differences in the expression of genes involved in fruit softening and ethylene biosynthetic pathways under different temperature storage conditions. Different peach cultivars of ‘Xiacui' and ‘Yumyeong', which are stonyhard, ‘Yinhualu', which is softmelting, ‘Hujing Milu', which is hard-melting, and ‘Baby Gold 6', which is non-melting at 80% ripening, were collected as test materials. The results showed that only slight ethylene production was detected after harvesting of ‘Yumyeong' and ‘Xiacui' under either a room temperature(25 °C) or low temperature(4 °C). The fruit firmness of stonyhard cultivars was retained at a high level under room temperature over time, whereas a low temperature induced ‘Yumyeong' fruit to soften. Quantitative real-time PCR results indicated that the PpACS1 gene was highly expressed in soft-melting, hard-melting and non-melting cultivars; however, expression was extremely low in stonyhard peaches. PpACS2 or PpACS3, however,was not detected in all five cultivars. Interestingly, cold treatment significantly decreased firmness along with endo-PG expression obviously upregulated in ‘Yumyeong', but not in ‘Xiacui' peaches. In conclusion, this study revealed that fruit softening of peaches with different flesh textures was closely related to ethylene biosynthesis during the storage period, which was controlled via regulating relevant gene expression levels under different storage temperatures. | YANG Yong MA Ruijuan ZHANG Binbin SONG Zhizhong ZHANG Chunhua GUO Shaolei YU Mingliang | 2016 | Horticultural Plant Journal2016，2，2： | 7 |
| 2 | Differentiation of embryonic versus adult rat neural stem cells into dopaminergic neurons in vitro显示文摘BACKGROUND:It has been reported that the conversion of neural stem cells into dopaminergic neurons in vitro can be increased through specific cytokine combinations. Such neural stem cell-derived dopaminergic neurons could be used for the treatment of Parkinson’s disease. However, little is known about the differences in dopaminergic differentiation between neural stem cells derived from adult and embryonic rats. OBJECTIVE: To study the ability of rat adult and embryonic-derived neural stem cells to differentiate into dopaminergic neurons in vitro. DESIGN: Randomized grouping design. SETTING: Department of Neurosurgery in the First Affiliated Hospital of Sun Yat-sen University. MATERIALS: This experiment was performed at the Surgical Laboratory in the First Affiliated Hospital of Sun Yat-sen University (Guangzhou, Guangdong, China) from June to December 2007. Eight, adult, male, Sprague Dawley rats and eight, pregnant, Sprague Dawley rats (embryonic day 14 or 15) were provided by the Experimental Animal Center of Sun Yat-sen University. METHODS: Neural stem cells derived from adult and embryonic rats were respectively cultivated in serum-free culture medium containing epidermal growth factor and basic fibroblast growth factor. After passaging, neural stem cells were differentiated in medium containing interleukin-1α, interleukin-11, human leukemia inhibition factor, and glial cell line-derived neurotrophic factor. Six days later, cells were analyzed by immunocytochemistry and flow cytometry. MAIN OUTCOME MEASURES: Alterations in cellular morphology after differentiation of neural stem cells derived from adult and embryonic rats; and percentage of tyrosine hydroxylase-positive neurons in the differentiated cells. RESULTS: Neural stem cells derived from adult and embryonic rats were cultivated in differentiation medium. Six days later, differentiated cells were immunoreactive for tyrosine hydroxylase. The percentage of tyrosine hydroxylase positive neurons was (5.6 ± 2.8)% and (17.8 ± 4.2)% for adult and embryonic cells, respectively, with a significant difference between the groups (P < 0.01). CONCLUSION: Neural stem cells from embryonic rats have a higher capacity to differentiate into dopaminergic neurons than neural stem cells derived from adult rats. | Chunlong Ke Baili Chen Shaolei Guo Chao Yang | 2008 | Neural Regeneration Research2008，3，8： | 6 |
| 3 | Identification and expression analysis of chlorogenic acid biosynthesis key gene PpHCT in peach显示文摘Shikimic acid/quinic acid hydroxy cinnamyl transferase(HCT)is one of the key enzymes in the phenylpropanoid pathway.However,the role of the HCT gene in chlorogenic acid(CGA)biosynthesis in peach fruit remains unclear.For this,we identified the accumulation pattern of CGA in four peach cultivars,cloned and characterized 11 PpHCT gene members,and further analyzed the expression patterns of these PpHCT genes during fruit development.The contents of CGAs in the four peach cultivars all exhibited a trend of increasing and then decreasing during the fruit growth and development.Moreover,the contents of CGAs in the peel and flesh were tissue-specific.Gene structure analysis indicated that the PpHCT genes were highly conserved,containing two exons and one intron.The protein structure analysis demonstrated that the PpHCT proteins contained two conserved motifs(HXXXD,DFGWG)and a transferase domain(PF02458),which belonged to the BAHD acyltransferase family.The cis-acting element analysis suggested that the promoters of PpHCT genes contained many light-related,hormone-related,stress-related,tissue-specific,and circadian-related elements,and they could participate in a variety of biological processes.Phylogenetic analysis showed that the HCT proteins of peach were closely related to the HCT proteins of plum and had a close evolutionary relationship.The qRT-PCR analysis indicated that the expression levels of PpHCT1 and PpHCT2 showed an opposite trend to the accumulation of CGA,whereas the expression levels of PpHCT4,PpHCT5,PpHCT7,PpHCT8,and PpHCT11 demonstrated the same trend as CGA accumulation.It was worth noting that only PpHCT4 and PpHCT5 were highly expressed in the two high-CGA cultivars but showed low levels of expression in the two low-CGA cultivars.Therefore,it was hypothesized that these two genes might be key genes to the synthesis of CGA in peach fruit.Those findings provide a theoretical basis for further study on the biological functions of the HCT gene and help to reveal the molecular mechanism of CGA. | Ziwen Su Meng Sun Zhixiang Cai Shaolei Guo Jiyao Li Bintao Zhao Zhijun Shen Ruijuan Ma Juan Yan Mingliang Yu | 2023 | Horticultural Plant Journal2023，9，4： | 0 |
| 4 | Mesoporous radiosensitized nanoprobe for enhanced NIR-Ⅱ photoacoustic imaging-guided accurate radio-chemotherapy显示文摘Oxygen deficiency is a major obstacle to hypoxic-related cancer theranostics,and developing the oxygen production nanoplatforms received the widespread attention.However,it is remaining a challenge to structure a nanoplatform with hypoxia alleviation effect and imaging-guided cancer radiotherapy.Herein,we present a novel theranostics nanoplatform(Au NPs/UCNPs/WO_(3)@C)comprising of tungsten trioxide(WO3)that loaded gold nanoparticles(Au NPs)and up-conversion nanoparticles(UCNPs)for improved photoacoustic(PA)imaging performance in the second near infrared window(NIR-Ⅱ,900-1,700 nm).Au NPs/UCNPs/WO_(3)@C exhibited superior oxygen-generation effect and doxorubicin loading capacity,thus serving as an efficient radiosensitizer for radio-chemo anti-cancer therapy.Importantly,the accumulated Au NPs/UCNPs/WO_(3)@C in the tumor region led to the increased NIR-Ⅱ PA imaging signal and the blood oxygen saturation signal,which could enhance radiation sensitivity and accurately guiding cancer radiotherapy to reduce side effects on normal tissues.This study with proof-of-concept confirmed the multifaceted characteristics and encouraging potential of biomimetic Au NPs/UCNPs/WO_(3)@C for NIR-Ⅱ PA imaging-guided tumor therapeutics. | Tao Chen Lichao Su Lisen Lin Xiaoguang Ge Feicheng Bai Meng Niu Chenlu Wang Jibin Song Shaolei Guo Huanghao Yang | 2022 | Nano Research2022，15，5： | 0 |
| 5 | A Large Acute Gastroenteritis Outbreak Associated with Both Campylobacter coli and Human Sapovirus—Beijing Municipality,China,2021显示文摘Summary What is already known about this topic?Campylobacter is a significant foodborne pathogen that leads to global outbreaks of acute gastroenteritis(AGE)usually affecting less than 30 individuals.Human sapovirus(HuSaV)is an enteric virus responsible for sporadic cases and outbreaks of AGE worldwide.In a study conducted in Beijing,HuSaV detection ranked second after norovirus.What is added by this report?We present a discussion of the first large-scale outbreak of AGE caused by both Campylobacter coli(C.coli)and HuSaV.The outbreak involved a total of 996 patients and exhibited two distinct peaks over a period of 17 days.Through case-control studies,we identified exposure to raw water from a secondary water supply system as a significant risk factor.Among 83 patients,49 samples tested positive for C.coli,39 samples tested positive for HuSaV,and 27 samples tested positive for both pathogens using real-time polymerase chain reaction detection.Furthermore,whole-genome sequencing of 17 C.coli isolates obtained from 17 patients revealed that all isolates belonged to a highly clonal strain of C.coli.What are the implications for public health practice?Outbreaks of AGE resulting from multiple pathogen infections warrant increased attention.This report emphasizes the significance of ensuring the safety of drinking water,particularly in secondary supply systems. | Lin Zou Ying Li Guilan Zhou Zhenzhou Huang Changyan Ju Chunyan Zhao Xiang Gao Bojun Zhen Ping Zhang Xiaochen Guo Jing Zhang Yang Zhang Bo Liu Shaolei Zhou Aixia Yan Ying Kang Yanchun Wang Hongmei Ma Xiaohui Li Maojun Zhang | 2023 | China CDC weekly2023，5，52： | 0 |
| 6 | Intelligent power distribution live‐line operation robot systems based on stereo camera显示文摘Maintenance tasks in distribution networks are often accompanied by hazards associated with high altitudes and high voltages.By utilising robots instead of human operators to perform these tasks,potential risks can be avoided,while productivity can be increased.This research proposes an intelligent power distribution live‐line operation robot(PDLOR)system based on a stereo camera to replace human to complete work.The PDLOR system consists of several key components,including dual manipulators,wireless tools,a visual perception system,an insulated bucket truck,and a ground control ter-minal.Once the task is confirmed,the real‐time vision system identification and posi-tioning enable the adjustment of the insulated bucket to position the robot correctly for its intended work.The stereo camera plays a crucial role in accurately recognising and estimating the object's orientation.Additionally,a simplified reconstruction is performed within a virtual simulation environment,which aids in collision detection during path planning.After obtaining the optimal path,it is then communicated to the real manip-ulator for execution.To validate the feasibility of the PDLOR system,field experiments were conducted in actual distribution network scenarios.The results demonstrate that the PDLOR effectively completes single‐phase power‐line connection tasks within a remarkable 10‐min timeframe. | Yutao Chen Yahao Wang Xuming Tang Kai Wu Shaolei Wu Rui Guo Yu Feng Erbao Dong | 2023 | High Voltage2023，8，6： | 0 |
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