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| 1 | MAPK-ERK在牙本质基质蛋白1信号通路中的作用显示文摘目的探讨在人骨髓间充质干细胞(hMSC)、小鼠前成骨细胞(MC3T3)和成牙本质细胞(MDPC-23)中,牙本质基质蛋白1(DMP1)是否通过激活丝裂原活化蛋白激酶(MAPK)-细胞外调节蛋白激酶(ERK)信号通路发挥作用。方法 Western blot检测不同时间点rDMP1C和rDMP1F蛋白处理hMSC、MC3T3-E1和MDPC-23后,对MAPK-ERK的激活情况;并检测使用MAPK抑制剂后,ERK的激活是否被抑制;细胞免疫荧光技术观察MAPK抑制剂抑制激活的ERK从胞浆向胞核的转位情况。Western blot检测siRNA沉默Ras基因后,对rDMP1引起MAPK-ERK信号通路激活的影响。结果三种细胞中,rDMP1F和rDMP1C在5 min^3 h均可以激活MAPK-ERK通路,而总ERK在各时间点均无显著变化;rDMP1F激活信号通路的持续时间均明显长于rDMP1C;MAPK抑制剂处理组的p-ERK条带与rDMP1F/C处理组的p-ERK条带差别明显。hMSC中,rDMP1F激活MAPK信号通路持续时间要长于其在MC3T3和MDPC-23中的作用。细胞免疫荧光检测发现,MAPK抑制剂组可以阻断ERK向细胞核内的转位。MC3T3和MDPC-23在siRNA沉默Ras基因后,ERK的磷酸化水平与rDMP1F单独处理组相比显著降低。结论 rDMP1C和rDMP1F都通过Ras激活MAPK-ERK信号通路,而rDMP1F比rDMP1C具有更强的信号功能。 | 伍虹 庄沛林 余艳崧 陈绛媛 Sfeir Charles 黄洪章 | 2013 | 中华口腔医学研究杂志(电子版)2013，7，6： | 2 |
| 2 | Evapotranspiration and seed yield of field grown soybean under deficit irrigation condi-tions显示文摘 | Fadi Karam Randa Masaad Therese Sfeir | 2005 | Agricultural Water Management2005，，75： | 1 |
| 3 | Telomere- end processing the terminal nucleotides of human chromosomes 显示文摘 | Sfeir A J Chai W Shay J W | 2005 | Mol Cell2005，18，1： | 1 |
| 4 | Telomere-end processing the terminal nucleotides of human chromosomes显示文摘 | Sfeir AJ Chai W Shay JW | 2005 | Mol Cell2005，18，： | 1 |
| 5 | POT1 protects telorneres from a transient DNA danzage response and determines how human chromosomes end显示文摘 | Hockemeyer D Sfeir A J Shay J W | 2005 | EMBO J2005，24，14： | 1 |
| 6 | Removal cf shelterin reveals the telomere, end-proletion problem 显示文摘 | Sfeir A de Iange T | 2012 | Sciene2012，336，6081： | 1 |
| 7 | Evaluation of remifentanil in endos-copic retro-grade cholangio-pancreatography显示文摘 | ELBITAR N SFEIR S | 2006 | Middle East J Anesthesiol2006，18，9： | 1 |
| 8 | Use of recombinant hu- man parathyroid hormone in hypocalcemic cardiomyopathy 显示文摘 | Ballane GT Sfeir JG Dakik HA | 2012 | Eur J Endocrinol2012，166，6： | 1 |
| 9 | Postoperative outcomes after laparoscopic splenectomy compared with open splenectomy显示文摘 | Musallam K M Khalife M Sfeir P M | 2013 | Ann Surg2013，257，6： | 1 |
| 10 | Investigation of three-dimensional turbulent rectangular jets显示文摘 | Sfeir A A | | 0，，10： | 1 |
| 11 | Telomere extension occurs at most chromosome ends and is uncoupled from fill-in human cancer cells显示文摘 | Zhao Y Sfeir AJ Zou Y | 2009 | Cell2009，138，3： | 1 |
| 12 | Mammalian telomeres resemble fragile sites and require TRF1 for efficient replication显示文摘 | Sfeir A Kosiyatrakul ST Hockemeyer D | | 0，，： | 1 |
| 13 | Prediction of the survival and functional ability of severe stroke patients after ICU therapeutic intervention显示文摘 | Riachy M Sfeir F Sleilaty G | 2008 | BMC Neurol2008，8，： | 1 |
| 14 | Nanostructured calcium phosphates (NanoCaPs) for non-viral gene delivery: Influence of the synthesis parameters on transfection efficiency显示文摘 | Dana Olton Jinhua Li Mary E. Wilson Todd Rogers John Close Leaf Huang Prashant N. Kumta Charles Sfeir | 2006 | Biomaterials2006，，6： | 1 |
| 15 | Extensive Hirschsprung's disease associated with intestinal malrotation显示文摘 | Corsois L Boman F Sfeir R | 2004 | Arch Pediatr2004，11，10： | 1 |
| 16 | Stability of calcium substituted lanthanum chromites used as SOFC anodes for methane oxidation显示文摘 | Sfeir J Herle J V McEvoy A J | 1999 | J European Ceramic Society1999，19，67： | 1 |
| 17 | LaCrO3-based anodes:stability considerations显示文摘 | Sfeir J | 2003 | J Power Sources2003，118，： | 1 |
| 18 | LaCrO3 -based anodes : stability considerations显示文摘 | Sfeir J | 2003 | Journal of Power Sources2003，118，： | 1 |
| 19 | Lanthanum chromite based catalysts for oxidation of methane directly on SOFC anodes显示文摘 | Sfeir J Buffat P A Mockli P | 2001 | Journal of Catalysis2001，202，： | 1 |
| 20 | Mammalian polymerase 0 promotes alternative NHEJ and suppresses recombination 显示文摘 | Mateos - Gomez PA Sfeir A | 2015 | Nature2015，518，7538： | 1 |
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