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| 1 | Effect of adipose-derived mesenchymal stem cells on hepatocellular carcinoma: In vitro inhibition of carcinogenesis显示文摘AIM To investigate the effect of adipose-derived mesenchymal stem cells(ADMSCs)and their conditioned media(CM) on hepatocellular carcinoma(HCC) cell tumorigenesis.METHODS The proliferation rate of HepG2 and PLC-PRF-5 HCC cancer cells was measured using the trypan blue exclusion method and confirmed using the cell-counting kit8(commonly known as CCK-8) assay. Apoptosis was detected by flow cytometry using annexin V-FITC. Protein and mRNA expression was quantified by ELISA and real time PCR, respectively. Migration and invasion rates were performed by Transwell migration and invasion assays. Wound healing was examined to confirm the data obtained from the migration assays.RESULTS Our data demonstrated that when co-culturing HCC cell lines with ADMSCs or treating them with ADMSC CM, the HCC cell proliferation rate was significantly inhibited and the apoptosis rate increased. The decreased proliferation rate was accompanied by an upregulation of P53 and Retinoblastoma mRNA and a downregulation of c-Myc and hTERT mRNA levels. More notably, ADMSCs and their CM suppressed the expression of the two important markers of HCC carcinogenicity, alpha-fetoprotein and Des-gamma-carboxyprothrombin. In addition, the migration and invasion levels of HepG2 and PLC-PRF-5 cells significantly decreased, potentially through increased expression of the tissue inhibitor metalloproteinases TIMP-1, TIMP-2 and TIMP-3.CONCLUSION These findings shed new light on a protective and therapeutic role for ADMSCs and their CM in controlling HCC invasiveness and carcinogenesis. | Rim Serhal Nagib Saliba George Hilal Mayssam Moussa Ghada S Hassan Oula El Atat Nada Alaaeddine | 2019 | World Journal of Gastroenterology2019，25，5： | 6 |
| 2 | Abdominal CT angiography before surgery as a predictor of postoperative death in acute aortic dissection 显示文摘 | Vernhet H Serfaty JM Serhal M | 2004 | AJR Am J Roentgenol2004，182，4： | 1 |
| 3 | Unexplained infertility: the value of Pergonal superovulation combined with intrauterine insemination 显示文摘 | Serhal PF Katz M Little V | 1988 | Fertil Steril1988，49，4： | 1 |
| 4 | Do ovarian reserve tests predict miscarriage in women undergoing assisted reproduction treatment?显示文摘 | Banchhita Sahu Ozkan Oztatrk Paul Serhal | 2010 | European Journal of Obstetrics & Gynecology and Reproductive Biology2010，153，： | 1 |
| 5 | MCAK regulates chromosome alignment but is not necessary for preventing aneuploidy in mouse oocyte meiosis I 显示文摘 | Illingworth C Pirmadjid N Serhal P | 2010 | Development2010，137，13： | 1 |
| 6 | Evaluation of the relationship between follicular fluid oxidative stress, ovarianhormones, and response to gonadotropin stimulation 显示文摘 | APPASAMY M JAUNIAUX E SERHAL P | 2008 | Fertility and Sterility2008，89，4： | 1 |
| 7 | Oocyte morphology predicts outcone of intracy toplasmic sperm injection显示文摘 | Serhal PF Raniere DM Kinis A | 1997 | Hum Reprod1997，12，12： | 1 |
| 8 | Cardiovascular surgery outcomes of patients with human immunodeficiency virus infection:new data in the era of combination antiretroviral therapy显示文摘 | Sullivan CE Serhal M Longenecker CT | 2015 | J Thorac Cardiovasc Surg2015，149，5： | 1 |
| 9 | The stereognostie ability of natural dentitions versus implant-supported fixed prostheses or overdentures显示文摘 | Jaeobs R Bou Serhal C van Steenberghe D | 1997 | Clin Oral Investig1997，1，2： | 1 |
| 10 | Sequential therapy regimen for Helicobacterpylori infection in children显示文摘 | Kalach N Serhal L Bergeret M | 2008 | Arch Pediatr2008，15，2： | 1 |
| 11 | Oocyte morphology predicts out come of intracytoplasmic sperm injection显示文摘 | Ranieri DM Dinis M | 1997 | Hum Reprod1997，12，6： | 1 |
| 12 | Evaluation of the relationship between follicular fluid oxidative stress,ovarian hormones,and response to gonadotropin stimulation显示文摘 | Appasamy M Jauniaux E Serhal P | 2008 | Fertil Steril2008，89，4： | 1 |
| 13 | Diagnostic yield of conventional and digital ephalometric images: a human cadaver study显示文摘 | Gijbels F Bou Serhal C Willems G | 2001 | Dentomaxillofac Radiol2001，30，2： | 1 |
| 14 | 显示文摘 | Serhal Z Morvan J Bercot P | 2001 | Surface & Coatings Technology2001，145，13： | 1 |
| 15 | Cytogenetic and Y chromosome microdeletion screening of a random group of infertile males显示文摘 | Svennevik E C Serhal P | 2003 | Fertil steril2003，79，2： | 1 |
| 16 | Anastomotic stricture after surgical repair of esophageal atresia: frequency, risk factors, and efficacy of esophageal bougie dilatations显示文摘 | Serhal L Gottrand F Sfeir R | 2012 | J Pediatr Surg2012，45，7： | 1 |
| 17 | Diagnostic yield of conventional and digital cephalometric images:a human cadaver study显示文摘 | Gijbels F Bou Serhal C Willems G | 2001 | Dentomaxillofac Radiol2001，30，2： | 1 |
| 18 | Adenomyosis reduces pregnancy rates in infertile women undergoing IVF显示文摘 | Rehan Salim Solon Riris Wael Saab Benjamin Abramov Iffat Khadum Paul Serhal | 2012 | Reproductive BioMedicine Online2012，，3： | 1 |
| 19 | Evaluation of the relationship between follicular fluid oxidative stress, ovarian hormones, and response to gonadotropin stimulation 显示文摘 | Appasamy M Jauniaux E Serhal P | 2008 | Fertil Steril2008，89，： | 1 |
| 20 | Unexplained infertility- the value of Pergonal superovulation combined with intrauterine insem ination 显示文摘 | Serhal PF Katz M Little V | 1988 | Fertil Steril1988，40，： | 1 |
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