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| 1 | Nitrative and oxidative DNA damage in intrahepatic cholangiocarcinoma patients in relation to tumor invasion显示文摘AIM: Nitrative and oxidative DNA damage such as 8-nitroguanine and 8-oxo-7,8-dihydro-2'-deoxyguanosine(8-oxodG) formation has been implicated in initiation and/or promotion of inflammation-mediated carcinogenesis.The aim of this study is to clarify whether these DNA lesions participate in the progression of intrahepatic cholangiocarcinoma.METHODS: We investigated the relation of the formation of 8-nitroguanine and 8-oxodG and the expression of hypoxia-inducible factor-1α (HIF-1α) with tumor invasion in 37 patients with intra-hepatic cholangiocarcinoma.RESULTS: Immunohistochemical analyses revealed that 8-nitroguanine and 8-oxodG formation occurred to a much greater extent in cancerous tissues than in non-cancerous tissues. HIF-1α could be detected in cancerous tissues in all patients, suggesting low oxygen tension in the tumors.HIF-1α expression was correlated with inducible nitric oxide synthase (iNOS) expression (r= 0.369 and P = 0.025)and 8-oxodG formation (r = 0.398 and P = 0.015).Double immunofluorescence study revealed that iNOS and HIF-1α co-localized in cancerous tissues. Notably, the formation of 8-oxodG was correlated significantly with lymphatic invasion (r = 0.386 and P= 0.018). Moreover, 8-nitroguanine and 8-oxodG in non-cancerous tissues were associated significantly with neural invasion (P = 0.042and P = 0.026, respectively). These results suggest that reciprocal activation between HIF-1α and iNOS mediates persistent DNA damage, which induces tumor invasiveness via mutations, resulting in poor prognosis.CONCLUSION: The formation of 8-nitroguanine and 8-oxodG plays an important role in multiple steps of genetic changes leading to tumor progression, including invasiveness. | Somchai Pinlaor Banchob Sripa Ning Ma Yusuke Hiraku Puangrat Yongvanit Sopit Wongkham Chawalit Pairojkul Vajarabhongsa Bhudhisawasdi Shinji Oikawa Mariko Murata Reiji Semba Shosuke Kawanishi | 2005 | World Journal of Gastroenterology2005，11，30： | 9 |
| 2 | Lymphoepithelioma-like esophageal carcinoma with macroscopic reduction显示文摘Esophageal lymphoepithelioma-like carcinoma(LELC) is extremely rare. We report the first case of esopha-geal LELC showing macroscopic reduction. A 67-year-old male presented with dysphagia and, by endoscopic examination, was found to have a significantly raised tumor of 10 mm in diameter in the thoracic esophagus. The biopsied material showed esophageal cancer. We performed endoscopic submucosal dissection. However, the tumor became flattened, similar to a scar, in only 2 mo. Histologically, the carcinoma cells had infiltrated the submucosal layer. Prominent infiltration of T lymphoid cells that stained positive for CD8 was observed aroundthe carcinoma cells. Therefore, this lesion was consid-ered to be an LELC with poorly differentiated squamous cells. Because the margin was positive, an esophagec-tomy was performed. Carcinoma cells were detected in the neck in one lymph node. The staging was T1N0M1 b. However, the patient has been well, without adjuvant therapy or recurrence, for more than 5 years. | Masaya Uesato Tuguaki Kono Tooru Shiratori Yasunori Akutsu Isamu Hoshino Kentarou Murakami Daisuke Horibe Tetsurou Maruyama Yoshihide Semba Ryuma Urahama Yukiko Ogura Takashi Oide Toru Tanizawa Hisahiro Matsubara | 2014 | World Journal of Gastrointestinal Endoscopy2014，6，8： | 6 |
| 3 | Cdx2 expression and its promoter methylation during metaplasia-dysplasia-carcinoma sequence in Barrett's esophagus显示文摘AIM:To examine how the expression of caudal type homebox transcription factor 2(Cdx2) is regulated in the development of malignancy in Barrett's esophagus.METHODS:Cdx2,mucin(MUC) series(MUC2,MUC5AC and MUC6),p53 and E-cadherin expression in Barrett's esophagus and adenocarcinoma specimens were examined by immunostaining.Isolated clusters of cells from(1) MUC2 and Cdx2-positive intestinal metaplastic mucosa;(2) MUC5AC and MUC6-positive,and MUC2 and Cdx2-negative high-grade dysplasia(HD),or intramucosal adenocarcinoma(IMC);and(3) MUC5AC,MUC6 and Cdx2-positive poorly-differentiated invasive adenocarcinoma(PDA) were analyzed by methylationspecific polymerase chain reaction using sets of primers for detecting methylation status of the Cdx2 gene.RESULTS:Most of the non-neoplastic Barrett's esophageal mucosa showing intestinal-type metaplasia with or without low-grade dysplasia was positive for E-cadherin,MUC series and Cdx2,but negative for p53.A portion of the low-grade to HD was positive for E-cadherin,MUC5AC,MUC6 and p53,but negative for MUC2 and Cdx2.The definite IMC area was strongly positive for MUC5AC,MUC6 and p53,but negative for MUC2 and Cdx2.Methylation of the Cdx2 promoter was not observed in intestinal metaplasia,while hypermethylation of part of its promoter was observed in hot dipped and IMC.Hypermethylation of a large fraction of the Cdx2 promoter was observed in PDA.CONCLUSION:Cdx2 expression is restored irrespective of the methylation status of its promoter.Apparent positive immunohistochemical results can be a molecular mark for gene silencing memory. | Kenji Makita Riko Kitazawa Shuho Semba Koto Fujiishi Miku Nakagawa Ryuma Haraguchi Sohei Kitazawa | 2013 | World Journal of Gastroenterology2013，19，4： | 5 |
| 4 | 超超临界锅炉用钢SUPER304H、TP347HFG和HR3C长期蠕变特性和微观组织研究显示文摘SUPER304H(18Cr-9Ni-3Cu—Nb—N,ASME CC2328)和TP347HFG(18Cr-12 Ni—Nb,ASME SA213)钢种,已经成功研制并用于制造高强度、高抗蒸汽氧化性能的钢管。SUPER304H在600℃最长蠕变时间为85,426小时和TP347HFG在700℃最长蠕变时间为55,858小时,这充分证明了其具有稳定的强度和微观组织,与其他传统的奥氏体不锈钢相比,即使在微观组织中发现含有很少量的σ相,但并没有观察到其它脆性相。HR3C(25Cr-20Ni—Nb—N,ASME CC2115)也已研制并用于具有高强度和高抗腐蚀性能的钢管。该钢管可应用于蒸汽温度为600℃左右的超超临界锅炉的易腐蚀部件。HR3C在700℃、69MPa的最长蠕变时间为88,362小时,这证明在600—800%它具有相当高的蠕变强度和稳定的微观组织。自1991年以来,这三种钢管已经作为过热器和再热器管安装在Eddystone三号超超临界炉上,而且经过长期服役后,割管取下进行微观组织研究。本文描述了这三种钢管的最新长时蠕变特性和长期时效后微观组织的变化。目前世界上已经将这_一种钢管作为标准材料成功用作新刊榴超临界锅炉的讨热器和再热器管。 | A. Iseda H. Okada H. Semba M. Igarashi 张学星(翻译) 李刚(校对) | 2009 | 东方锅炉2009，，1： | 4 |
| 5 | Phosphorylation of Akt/PKB is required for suppression of cancer cell apoptosis and tumor progression in human colorectal carcinoma 显示文摘 | Itoh N Semba S Ito M | 2002 | Cancer2002，94，12： | 2 |
| 6 | Tissue distribution and immtmocytochemical localization of neurotrophin - 3 in the brain and peripheral tissure of rats 显示文摘 | Katoh - Semba R Kaisho Y Shintani A | 1996 | J Neruochtma1996，66，1： | 1 |
| 7 | Hyperphosphorylation of a novel 80 kDa protein-tyrosine kinase similar to Ltk in a human Ki-1lymphoma cell line,AMS3显示文摘 | Shiota M Fujimoto J Semba T | 1994 | Oncogene1994，9，： | 1 |
| 8 | Transluminal placement of endovascular stent-grafts for the treatment of descending thoracic aortic aneurysms显示文摘 | Dake MD Miller DC Semba CP | 1994 | N Engl J Med1994，331，26： | 1 |
| 9 | Transcriptional activity of Testis-determining factor SRY is modulated by the Wilms'tumor 1 gene product,WT1显示文摘 | Matsuzawa-Watanabe Y Inoue J Semba K | 2003 | Oncogene2003，22，39： | 1 |
| 10 | Maternal vitamin A deficiency and mother-to-child transmission of HIV-1显示文摘 | Semba RD Miotti PG Chiphangwi JD | 1994 | Lancet1994，343，： | 1 |
| 11 | The role of vitamin A and related retinoids in immune function 显示文摘 | Semba RD | 1998 | Nutr Rev1998，56，12： | 1 |
| 12 | Gastric and intestinal claudin expression at the invasive front of gastric carcinoma 显示文摘 | MATSUDA Y SEMBA S UEDA J | 2007 | Cancer Sci2007，98，7： | 1 |
| 13 | Transluminal placement of endovascular stent-grafts for the treatment of descending thoracic aortic aneurysms显示文摘 | DAKE M D MILLER D C SEMBA C P | 1994 | N Engl J Med1994，331，26： | 1 |
| 14 | Iliofemoral deep venous thrombosis:aggressive therapy with catheter-directed thrombolysis显示文摘 | Semba CP Dake MD | | 0，，02： | 1 |
| 15 | Significance of AKT phos- phorylation on tumor growth and vascular endothelial growth factor expression in human gastriccar cinoma 显示文摘 | KobayashiI Semba S Matsuda Y | 2006 | Patho Biol2006，73，1： | 1 |
| 16 | Significance of Akt phosphorylation on tumor growth and vascular endothelial growth factor expression in human gastric carcinoma显示文摘 | Kobayashi I Semba S Matsuda Y | 2006 | Pathobiology2006，73，： | 1 |
| 17 | Microsatellite instability in precancerous lesions and adenocarcinomas of the stomach 显示文摘 | Yokozaki H Yamamoto S | 1996 | Cancer1996，77，8： | 1 |
| 18 | Current status of three-di- mensional spiral CT scanning for imaging the vasculuture 显示文摘 | Rubin G D Eake M D Semba C P | 1995 | Radiol Clin North Am1995，33，1： | 1 |
| 19 | Transluminal placement of endovascular sent-grafts for treatment of descending thoracic aortic aneurysms显示文摘 | Dake MD Miller C Semba CP | 1994 | N Engl J Med1994，331，26： | 1 |
| 20 | BRAF/K-ras mutation, microsatellite, instability, and promoter hypemethylation of hMLH1/MGMT in human gastric carcinomas显示文摘 | Wu M Semba S Oue N | 2004 | Gastric Cancer2004，7，6： | 1 |
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