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| 1 | Recombinant amelogenin regulates the bioactivity of mouse cementoblasts in vitro显示文摘Amelogenin(AMG) is a cell adhesion molecule that has an important role in the mineralization of enamel and regulates events during dental development and root formation. The purpose of the present study was to investigate the effects of recombinant human AMG(rhAMG) on mineralized tissue-associated genes in cementoblasts. Immortalized mouse cementoblasts(OCCM-30)were treated with different concentrations(0.1, 1, 10, 100, 1 000, 10 000, 100 000 ng · mL^(-1)) of recombinant human AMG(rhAMG)and analyzed for proliferation, mineralization and mRNA expression of bone sialoprotein(BSP), osteocalcin(OCN), collagen type I(COL I), osteopontin(OPN), runt-related transcription factor 2(Runx2), cementum attachment protein(CAP), and alkaline phosphatase(ALP) genes using quantitative RT-PCR. The dose response of rhAMG was evaluated using a real-time cell analyzer.Total RNA was isolated on day 3, and cell mineralization was assessed using von Kossa staining on day 8. COL I, OPN and lysosomalassociated membrane protein-1(LAMP-1), which is a cell surface binding site for amelogenin, were evaluated using immunocytochemistry. F-actin bundles were imaged using confocal microscopy. rhAMG at a concentration of 100 000 ng · mL^(-1) increased cell proliferation after 72 h compared to the other concentrations and the untreated control group. rhAMG(100 000 ng · mL^(-1)) upregulated BSP and OCN mRNA expression levels eightfold and fivefold, respectively. rhAMG at a concentration of 100 000 ng · mL^(-1) remarkably enhanced LAMP-1 staining in cementoblasts. Increased numbers of mineralized nodules were observed at concentrations of 10 000 and 100 000 ng · mL^(-1) rhAMG. The present data suggest that rhAMG is a potent regulator of gene expression in cementoblasts and support the potential application of rhAMG in therapies aimed at fast regeneration of damaged periodontal tissue. | Sema S.Hakki S.Buket Bozkurt Emre Türkay Michel Dard Nuhan Purali Werner Gotz | 2018 | International Journal of Oral Science2018，10，2： | 3 |
| 2 | Separation of IPA/water mixtures by pervaporation: sorption and pervaporation results显示文摘 | Nilufer D H Sema T | 2004 | Vacuum2004，72，： | 1 |
| 3 | Separation of IPA/water mixtures by pervaporation: sorption and pervaporation results 显示文摘 | Nilufer D H Sema T | 2004 | Vacuum2004，72，： | 1 |
| 4 | Comprehensive mass transfer and reaction kinetics studies of CO2 absorption into aqueous solutions of blended MDEA-MEA 显示文摘 | Sema T Naami A Fu K | 2012 | Chemical Engineering Journal2012，209，15： | 1 |
| 5 | Comprehensive mass transfer and reaction kinetics studies of a novel reactive 4-diethylamino-2-butanol solvent for capturing CO2显示文摘 | Sema T Naami A Fu K | 2013 | Chemical Engineering Science2013，100，30： | 1 |
| 6 | Effect of Angiotensin-converting Enzyme Inhibition on Experimental Hepatic Fibrogenesis显示文摘 | Cansel Türkay ?zlem Y?nem Sema Ar?c? Ayhan Koyuncu Mehmet Kanbay | 2008 | Digestive Diseases and Sciences2008，，3： | 1 |
| 7 | How does sodium sulfate crystallize? Implications for the decay and testing of building materials显示文摘 | FEVZIYE A FIKRET T SEMA K | 2000 | Cem Concr Res2000，30，： | 1 |
| 8 | Preparation of uniform }'-FezOa particles with nanometer size by spray pyrolysis 显示文摘 | GONZALEZ-CARRENO T MORALES M P SEMA C J0 | 1993 | Materials Letters1993，18，15： | 1 |
| 9 | Pervaporation of MTBE/methanol mixtures through PVA membrane显示文摘 | Nilufer D H Sema T | 2004 | Desalination2004，160，3： | 1 |
| 10 | Caudalanesthesia forminor subumbilical pediatric surgery: a comparison of levobupivacaine aloneand levobupivacaine plus sufentanil显示文摘 | Atilla E Aybars T Sema T | 2008 | J Clin Anesth2008，20，4： | 1 |
| 11 | Structural characteristics of uniform y-Fe203 particles with different axial (length/width) ratios 显示文摘 | MORALES M P PECHARROMAN C GONZALEZ T SEMA C J | 1994 | J Solid State Chem1994，108，： | 1 |
| 12 | Magnetic Behavior of γ- Fe2 O3 nanocrystals dispersed in colloidal silica particles显示文摘 | Tartaj P Gonzalez-Carrefio T Sema C J | 2003 | J Phys Chem B2003，107，： | 1 |
| 13 | Biosorption of Hg( Ⅱ) and CA( Ⅱ ) from aqueous solutions: comparison of biosorptive capacity of algi- hate and immobilized live and heat inactivated Phanerochaete chrysospo- rium 显示文摘 | Kacar Y Cigdem A Sema T | 2002 | Process Biochemistry2002，37，6： | 1 |
| 14 | Investiga-tion of combustion kinetics of treated and untreatedwaste wood samples with thermogravimetric analysis显示文摘 | Yorulmaz Sema Yurdakul Atimtay Aysel T | 2009 | Fuel Processing Technology2009，90，78： | 1 |
| 15 | A phase i dose escalation and pharmacodynarnie study of SU5416 (Semaxanib) combined with weekly cisplatin and irinotecan in patients with advanced solid tumors 显示文摘 | Martin LK Bekaii-Saab T Sema D | 2013 | Onkologie2013，36，11： | 1 |
| 16 | Mass Transfer of CO2 Absorption in Hybrid MEA-Methanol Solvents in Packed Column 显示文摘 | Sema T Naami A Usubharatana P | 2013 | Energy Procedia2013，37，88： | 1 |
| 17 | Arsenic adsorption from aqueous solutions by activated red mud显示文摘 | LTUNDOGAN H S SEMA A FIKRET T | 2002 | Waste Management2002，22，： | 1 |
| 18 | Chemical composition of rainwater at EMEP station in Ankara, Turkey 显示文摘 | Sema T Incecik S Atimtay A T | 2002 | Atmospheric Research2002，65，： | 1 |
| 19 | Effect of dexmedetomidine on haemodynamic responses to laryngoscopy and intubation:perioperative haemodynamics and anaesthetic requirements显示文摘 | Munise Y Aybars T Sema T | 2006 | Drugs in R&D2006，7，1： | 1 |
| 20 | Cranial surgery and navigation with a compact intraoperative MRI system显示文摘 | Schulder M Semas T J Carmel PW | 2003 | Acta Neurochir Suppl2003，85，： | 1 |
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