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| 1 | Effect of human autologous serum and fetal bovine serum on human corneal epithelial cell viability,migration and proliferation in vitro显示文摘AIM: To analyze the concentration-dependent effects of autologous serum(AS) and fetal bovine serum(FBS) on human corneal epithelial cell(HCEC) viability, migration and proliferation.METHODS: AS was prepared from 13 patients with nonhealing epithelial defects Dulbecco's modified eagle medium/Ham's F12(DMEM/F12) with 5% FBS, 0.5% dimethyl sulphoxide(DMSO), 10 ng/m L human epidermal growth factor, 1% insulin-transferrin-selenium, then were incubated in serum media: DMEM/F12 supplemented by 5%, 10%, 15% or 30% AS or FBS. HCEC viability was analyzed using cell proliferation kit XTT, migration using a wound healing assay, proliferation by the cell proliferation enzyme-linked immunosorbent assay(ELISA) Brd U kit. Statistical analysis was performed using the generalized linear model, the values at 30% AS or 30% FBS were used as the baselines.RESULTS: HCEC viability was the highest at 30% AS or 15% FBS and the lowest at 10% AS or 30% FBS application. HCEC migration was the quickest through 30% AS or 30% FBS and the slowest through 5% AS or 5% FBS concentrations. Proliferation was the most increased through 15% AS or 5% FBS and the least increased through 30% AS or 30% FBS concentrations. HCEC viability at 10% and 15% AS was significantly worse(P=0.001, P=0.023) compared to baseline and significantly better at 15% FBS(P=0.003) concentrations. HCEC migration was significantly worse(P≤0.007) and HCEC proliferation significantly better(P<0.001) in all concentration groups compared to baseline.CONCLUSION: For the best viability of HCEC 30% AS or 15% FBS, for HCEC migration 30% AS or 30% FBS, for proliferation 15% AS or 5% FBS should be used. Therefore, we suggest the use of 30% AS in clinical practice. | Ming-Feng Wu Tanja Stachon Berthold Seitz Achim Langenbucher Nóra Szentmáry | 2017 | International Journal of Ophthalmology(English edition)2017，10，6： | 7 |
| 2 | Sex hormones and dry eye 显示文摘 | Schirra F Seitz B Knop N | 2009 | Oph-thalmologe2009，106，11： | 1 |
| 3 | Inducible expression of tissue factor in small-cell lung cancer: impact on morphology and matrix metalloproteinase secretion 显示文摘 | Hahn N Heiden M Seitz R | 2012 | J Cancer Res Clin Oncol2012，138，4： | 1 |
| 4 | Photo tourism: exploring photo collections in 3D显示文摘 | SNAVELY N SEITZ S M SZELISKI R | 2006 | ACM Trans on Graphics2006，25，3： | 1 |
| 5 | A theory of shape by space carving显示文摘 | S M Seitz | 2000 | International Journal of Computer Vision2000，38，3： | 1 |
| 6 | lSF-Fluoro- deoxyglucose-PET/CT to evaluate tmnor, nodal disease, and gross tumor volume of oropharyngeal and oral cavity cancer: Compari- son with MR imaging and validation with surgical specimen显示文摘 | Seitz O Chambron-Pinho N Middendorp M | 2009 | Neuroradiology2009，51，10： | 1 |
| 7 | Modeling the world from inter- net photo collections显示文摘 | Snavely N Seitz S M Szeliski R | 2008 | International Journal of Computer Vision2008，80，2： | 1 |
| 8 | The modular multisensory DLR-HIT-Hand显示文摘 | LIU H MEUSEL P SEITZ N | 2007 | Mechanism and Machine Theory2007，42，： | 1 |
| 9 | A theory of shape by space carving 显示文摘 | Kutulakos K N Seitz S M | 2000 | International Journal of Computer Vision2000，38，3： | 1 |
| 10 | A Theory of Shape by Space Carv- ing显示文摘 | Kutulakos K N Seitz S M | 2000 | Intemational Journal of Computer Vision2000，38，3： | 1 |
| 11 | Photo tourism : exploring pho- to collection in 3D 显示文摘 | SNAVELY N SEITZ S M SZELISKI R | 2006 | ACM Trans on Graphics2006，25，3： | 1 |
| 12 | Complications and early postoperative outcome after open prostatectomy in patients with benign prostalic enlargement: results of a prospective muhicenter study显示文摘 | Gratzke C Schlenker B Seitz M Karl A Hermanek P Lack N | 2007 | J Urol2007，177，4： | 1 |
| 13 | The modular multisensory DLR-HIT-Hand显示文摘 | Liu H Meusel P Seitz N | 2007 | Mechanism and Machine Theory2007，，42： | 1 |
| 14 | The modular multisensory DLRP- HIT - hand显示文摘 | Liu H Meusel P Seitz N | 2007 | Mechanism and Machine Theory2007，42，5： | 1 |
| 15 | Photo tourism: Exploring photo collections in 3D 显示文摘 | Snavely N Seitz S M Szeliski R | 2006 | ACM Transactions on Graphics (S0730-0301)2006，25，3： | 1 |
| 16 | Inducible expression of tis- sue factor in small-ceil lung cancer impact on morphology and matrix metalloproteinase seeretion显示文摘 | Hahn N Heiden M Seitz R | 2012 | J Cancer Res Clin On- coi2012，138，4： | 1 |
| 17 | The dependence of calcium efflux from cardiac muscle on temperature and external ion composition显示文摘 | Seitz N | 1968 | J Physiol1968，195，45： | 1 |
| 18 | A review of epidemio|ogical evidence for general anesthesia as a risk factor for Alzheimer'sdisease显示文摘 | Seitz DP Reimer CL Siddiqui N | 2013 | Prog Neuropsychophannacol Biol Psychiatry2013，47，： | 1 |
| 19 | Prevalence of psychiatric disorders among older adults in long-term care homes:a systematic review显示文摘 | Seitz D Purandare N Conn D | 2010 | Int Psychogeriatr2010，22，7： | 1 |
| 20 | A theory of shape by space carving显示文摘 | Kutulakos K N Seitz S M | 2000 | International Journal of Computer Vision2000，38，3： | 1 |
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