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| 1 | Human small intestine is capable of restoring barrier function after short ischemic periods显示文摘AIM To assess intestinal barrier function during human intestinal ischemia and reperfusion(IR).METHODS In a human experimental model,6 cm of jejunum was selectively exposed to 30 min of ischemia(I) followed by 30 and 120 min of reperfusion(R). A sham procedure was also performed. Blood and tissue was sampled at all-time points. Functional barrier function was assessed using dual-sugar absorption tests with lactulose(L) and rhamnose(R). Plasma concentrations of citrulline,an amino acid described as marker for enterocyte function were measured as marker of metabolic enterocytes restoration. Damage to the epithelial lining was assessed by immunohistochemistry for tight junctions( TJs),by plasma marker for enterocytes damage(I-FABP) and analyzed by electron microscopy(EM) using lanthanum nitrate as an electrondense marker.RESULTS Plasma L/R ratio's were significantly increased after 30 min of ischemia(30 I) followed by 30 min of reperfusion(30 R) compared to control(0.75 ± 0.10 vs 0.20 ± 0.09,P < 0.05). At 120 min of reperfusion(120 R),ratio's normalized(0.17 ± 0.06) and were not significantly different from control. Plasma levels of I-FABP correlated with plasma L/R ratios measured at the same time points(correlation: 0.467,P < 0.01). TJs staining shows distortion of staining at 30 I. An intact lining of TJs was again observed at 30 I120 R. Electron microscopy analysis revealed disrupted TJs after 30 I with paracellular leakage of lanthanum nitrate,which restored after 30 I120 R. Furthermore,citrulline concentrations closely paralleled the histological perturbations during intestinal IR.CONCLUSION This study directly correlates histological data with intestinal permeability tests,revealing that the human gut has the ability of to withstand short episodes of ischemia,with morphological and functional recovery of the intestinal barrier within 120 min of reperfusion. | Dirk HSM Schellekens Inca HR Hundscheid Claire AJI Leenarts Joep Grootjans Kaatje Lenaerts Wim A Buurman Cornelis HC Dejong Joep PM Derikx | 2017 | World Journal of Gastroenterology2017，23，48： | 4 |
| 2 | The diagnostic properties of rheumatoid arthritis recognizing a cyclic eitrul-linated peptide 显示文摘 | Schellekens GA Visser H Jong BA | 2005 | Arthritis Rheum2005，43，1： | 1 |
| 3 | Manufacture and quality control of biopharmaceuticals显示文摘 | Schellekens H | 2008 | Revista Espa''nola de Econom la de la Salud2008，，6： | 1 |
| 4 | Contact temperature and erosion in high-current diffuse vacuum arcs on axial magnetic field contacts显示文摘 | Schellekens H Schulman M B | 2001 | IEEE Trans on Plasma Science2001，29，3： | 1 |
| 5 | Biogenerics:the off-patent biotech products 显示文摘 | SCHELLEKENS H RYFFB JC | 2002 | Trends Pharmacol Sci2002，23，3： | 1 |
| 6 | Sufentanil a very potent and extremely safe intravenous morphine-like compound in mice, rats and dogs显示文摘 | Niemegeers DJE Schellekens KHI van Bever WFE | 1976 | Arzneim Forsch1976，26，： | 1 |
| 7 | The diagnostic properties of rheumatoid arthritis antibodies recognizing a cyclic citrullinated peptide显示文摘 | Schellekens GA Visser H de Jong BA | | 0，，1： | 1 |
| 8 | Immunogenicity of biopharmaceuticals显示文摘 | Kessler M Goldsmith D Schellekens H | 2006 | Nephrol Dial Transplant2006，21，5： | 1 |
| 9 | The incidence of Bordetella pertussis infections estimated in the population from a combination of serological surveys显示文摘 | de Melker HE Versteegh FG Schellekens JF | 2006 | J Infect2006，53，2： | 1 |
| 10 | New research issues in sous-vide cooking 显示文摘 | Mia Schellekens | 1996 | Food Science1996，，7： | 1 |
| 11 | The diagnostic properties of rheumatoid arthritis antibodies recognizing a cyclic citrullinated peptide 显示文摘 | Schellekens GA Visser H de Jong BAW | 2000 | Arthritis Rheum2000，，： | 1 |
| 12 | The diagnostic properties of rheumatoid arthritis antibodies recognizing a cyclic citrullinated peptide显示文摘 | Schellekens G A Visser H de Jong B A | 2000 | Arthritis Rheum2000，43，1： | 1 |
| 13 | Sufentanil, a very potent and extremely safe intravenous morphine-like compound in mice, rats and dogs 显示文摘 | NIEMEGEERS D J E SCHELLEKENS K H L VAN BEVER W F E | 1976 | Arzneim Forsch1976，26，10： | 1 |
| 14 | Patteras ofresting state connectivity in human primary visual cortical areas:a 7TfMRI study显示文摘 | Raemaekers M Schellekens W van Wezel RJA | 2014 | Neuroimage2014，，84： | 1 |
| 15 | Citrulline is an essential constituent of antigenic determinants recognized by rheumatoid arthritis-specific autoantibodies 显示文摘 | Schellekens GA de Jong BAW van den Hoogen FHJ | 1998 | J Clin Invest1998，101，： | 1 |
| 16 | Sufentanil,a very potent and extremely safe intravenous morphine-like compound in mice,rats and dogs显示文摘 | Niemegeers CJ Schellekens KH Van Bever WF | 1976 | Arzneimittelforschung1976，26，8： | 1 |
| 17 | Citrulline is an essential constituent of antigenic determinants recognized by rheumatoid arthritis speci-fic autoantibodies显示文摘 | Schellekens G A de Jong B A van den Hoogen F H | 1998 | Clin Invest1998，101，1： | 1 |
| 18 | Concurrent validity of questionnaire and performance-based disability measurements in patients with chronic nonspecific low back pain显示文摘 | Reneman M F Jorritsma W Schellekens J M | 2002 | Occup Rehabil2002，12，3： | 1 |
| 19 | Litrulline is an essential coustituent of antigenil determinants relogniud by rheumatoid arthritis-specific autoantibodies显示文摘 | Schellekens GA De Fong BA Van den Hoogen FH | 1998 | Jchin Invest1998，101，： | 1 |
| 20 | 查看详情显示文摘 | Brouwer H D Schellekens M A J Klumperman B Monteiro M J German A L | | 0，，： | 1 |
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