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| 1 | Pathological changes in the cellular structures of retina and choroidea in the early stages of alloxan-induced diabetes显示文摘AIM To investigate the temporal sequence of pathological changes in the cellular structures of retina and choroidea in the early stages of diabetes in laboratory animals.METHODS Experimental type 1 diabetes was modeled by three intraperitoneal injections of an alloxan solution into 30 male nonlinear rats at 16 wk of age. The 30 th and 60 th days from the final alloxan injection were chosen as the endpoints. Light and electron microscopy and morphometric and immunohistochemical studies were performed on histological slices of eyeballs from experimental animals.RESULTS Diabetic disturbances progressed to 60 d of the experiment. Thus, in the retina, a partial destruction of photoreceptors accompanied by interstitial edema was observed. The morphometric analysis revealed a reduction in the thickness of the retina. A reduction in the number of blood vessels of the choroid with disturbances of the endothelial cells and the vascular walls and a persistent reduction in the number of melanocytes were observed. The number of proliferating Ki-67 positive cells decreased, and the number of macrophages increased with diabetes development.CONCLUSION The starting point in the development of destructive changes involves early reduction in the number of melanocytes of the choroidea and alterations in the retinal pigment epithelium. | Irina Danilova Svetlana Medvedeva Svetlana Shmakova Margarita Chereshneva Alexey Sarapultsev Petr Sarapultsev | 2018 | World Journal of Diabetes2018，9，12： | 6 |
| 2 | Multifaceted functions of STING in human health and disease:from molecular mechanism to targeted strategy显示文摘Since the discovery of Stimulator of Interferon Genes(STING)as an important pivot for cytosolic DNA sensation and interferon(IFN)induction,intensive efforts have been endeavored to clarify the molecular mechanism of its activation,its physiological function as a ubiquitously expressed protein,and to explore its potential as a therapeutic target in a wide range of immune-related diseases.With its orthodox ligand 2’3’-cyclic GMP–AMP(2’3’-cGAMP)and the upstream sensor 2’3’-cGAMP synthase(cGAS)to be found,STING acquires its central functionality in the best-studied signaling cascade,namely the cGAS–STING–IFN pathway.However,recently updated research through structural research,genetic screening,and biochemical assay greatly extends the current knowledge of STING biology.A second ligand pocket was recently discovered in the transmembrane domain for a synthetic agonist.On its downstream outputs,accumulating studies sketch primordial and multifaceted roles of STING beyond its cytokine-inducing function,such as autophagy,cell death,metabolic modulation,endoplasmic reticulum(ER)stress,and RNA virus restriction.Furthermore,with the expansion of the STING interactome,the details of STING trafficking also get clearer.After retrospecting the brief history of viral interference and the milestone events since the discovery of STING,we present a vivid panorama of STING biology taking into account the details of the biochemical assay and structural information,especially its versatile outputs and functions beyond IFN induction.We also summarize the roles of STING in the pathogenesis of various diseases and highlight the development of small-molecular compounds targeting STING for disease treatment in combination with the latest research.Finally,we discuss the open questions imperative to answer. | Zili Zhang Haifeng Zhou Xiaohu Ouyang Yalan Dong Alexey Sarapultsev Shanshan Luo Desheng Hu | 2023 | Signal Transduction and Targeted Therapy2023，8，1： | 2 |
| 3 | New insights in to the treatment of myocardial infarction 显示文摘 | SARAPULTSEV P CHUPAKHIN O SARAPULTSEV A | 2012 | Int J Exp Patho12012，93，1： | 1 |
| 4 | New insights into the treatment of myocardial infarction 显示文摘 | SARAPULTSEV P CHUPAKHIN O SARAPULTSEV A | 2012 | Int J Exp Pathol2012，93，1： | 1 |
| 5 | New insights in to the treatment of myocardial infarction显示文摘 | Sarapultsev P Chupakhin O Sarapultsev A | | 0，，01： | 1 |
| 6 | New insights in to the treatment of myocardial infarction显示文摘 | Sarapultsev P Chupakhin O Sarapultsev A | | 0，，01： | 1 |
| 7 | Beneficial Effects of Celastrol on Immune Balance by Modulating Gut Microbiota in Experimental Ulcerative Colitis Mice显示文摘Ulcerative colitis(UC)is a chronic inflammatory bowel disease caused by many factors including colonic inflammation and microbiota dysbiosis.Previous studies have indicated that celastrol(CSR)has strong anti-inflammatory and immune-inhibitory effects.Here,we investigated the effects of CSR on colonic inflammation and mucosal immunity in an experimental colitis model,and addressed the mechanism by which CSR exerts the protective effects.We characterized the therapeutic effects and the potential mechanism of CSR on treating UC using histological staining,intestinal permeability assay,cytokine assay,flow cytometry,fecal microbiota transplantation(FMT),16S rRNA sequencing,untargeted metabolomics,and cell differentiation.CSR administration significantly ameliorated the dextran sodium sulfate(DSS)-induced colitis in mice,which was evidenced by the recovered body weight and colon length as well as the decreased disease activity index(DAI)score and intestinal permeability.Meanwhile,CSR down-regulated the production of pro-inflammatory cytokines and up-regulated the amount of anti-inflammatory mediators at both mRNA and protein levels,and improved the balances of Treg/Th1 and Treg/Th17 to maintain the colonic immune homeostasis.Notably,all the therapeutic effects were exerted in a gut microbiota-dependent manner.Furthermore,CSR treatment increased the gut microbiota diversity and changed the compositions of the gut microbiota and metabolites,which is probably associated with the gut microbiota-mediated protective effects.In conclusion,this study provides the strong evidence that CSR may be a promising therapeutic drug for UC. | Mingyue Li Weina Guo Yalan Dong Wenzhu Wang Chunxia Tian Zili Zhang Ting Yu Haifeng Zhou Yang Gui Kaming Xue Junyi Li Feng Jiang Alexey Sarapultsev Huafang Wang Ge Zhang Shanshan Luo Heng Fan Desheng Hu | 2022 | Genomics, Proteomics & Bioinformatics2022，20，2： | 1 |
| 8 | Radiotaxonomy of higher plants at the dormant seed stage 显示文摘 | Sarapultsev B I Geraskin S A | 1989 | Radiobiologiya1989，29，： | 1 |
| 9 | Species radioresistance and phylogenesis of higher plants 显示文摘 | Sarapultsev B I Geraskin S A | 1989 | Radiobiologiya1989，29，： | 1 |
| 10 | A one-stage model of experimental acute necrotizing pancreatitis in rats显示文摘AIM:To establish a one-stage model of experimental acute necrotizing pancreatitis(ANP)in rats characterized by the simplicity of performance and a high degree of repeatability.METHODS:ANP modeling in rats was performed based on modification of the ligation model as follows:synthetic material ligature using an atraumatic needle was performed to capture pancreatic gland ducts and marginal duodenum vessels.Ligature tips were exteriorized to the abdominal wall,and the ligature was skinned over to avoid catching intestine loops.Pancreatic macroscopic appearance and histological changes were observed.Blood biochemical and hemostatic indicators were also determined.RESULTS:Laboratory analysis of rats with experimental ANP showed a pattern of disturbances similar to that observed during pancreatic necrosis in humans as soon as the first day.General blood analysis revealed enhanced leukocytosis and alterations in leukogram characteristics,indicating acute inflammation.Serum levels of amylase,aspartate aminotransferase and creatinine significantly increased(P<0.05).Hemostatic indicators showed alterations indicating formation of disseminated intravascular coagulation,and signs of endotoxicosis were observed.These typical pancreatic necrosis patterns of disturbances were validated by the results of histological investigation.CONCLUSION:Histological changes and laboratory indicators confirm the development of a suitable model of ANP. | SARAPULTSEV Alexey RANTSEV Maxim SARAPULTSEV Petr MEDVEDEVA Svetlana DANILOVA Irina | 2013 | 中国病理生理杂志2013，29，10： | 0 |
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