|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | 小麦农杆菌介导转基因植株的稳定获得和检测显示文摘以预培养 4 d的小麦幼胚愈伤组织为受体 ,L B培养基上激活培养 3 d或 AB培养基上激活培养 4 d,并在WCC培养基中重悬的 C5 8c1农杆菌菌系为供体 ,将含有目的基因、标记基因的 p PTN2 4 9、p PTN 2 5 4、p PTN2 70、p SIS-GFP等重组 DNA质粒转入了小麦 ,经在含 10~ 5 0 mg/ L geneticin( G4 18)、5 0 mg/ L cefotaxime、5 0 m g/ L vancomycin、5 0 m g/ L ticillicin选择培养基上多次选择 ,移栽前利用 npt EL ISA检测 ,移栽后利用叶片离体退绿、PCR和 South-ern blot 4种方法综合检测 ,共获得了 2 9株转基因植株 ,转化频率平均为 0 .82 %。在所获得的转基因植株中 ,单拷贝整合植株占 65 .5 2 %。研究结果还表明 ,不同基因型的转化效率显著不同 ,除了 Bobwhite外 ,笔者也从扬麦 10号获得了转基因植株 ;EL ISA测定值的高低与外源 DNA整合的拷贝数有一定关系 ;初步建立了农杆菌介导稳定转化小麦的技术体系。 | 叶兴国 Shirley Sato 徐惠君 杜丽璞 Tom Clemente | 2001 | 中国农业科学2001，34，5： | 66 |
| 2 | 利用烟杆制造纤维板的研究显示文摘我们已成功地利用烟杆制成纤维板,这种纤维板符合菲律宾标准协会制订的标准。批量生产表明,用烟杆生产的纤维板比用木材生产的纤维板便宜。目前,私人企业和政府部门正在积极合作,以便使此技术投入工业应用。 | 刘立全 Reynaldo C.Castro Shirley C.Agrupis Julie L.Lorenzo | 2000 | 烟草科技2000，33，7： | 17 |
| 3 | BCL、RIP细胞凋亡基因向小麦中的导入和赤霉病抗性鉴定显示文摘利用农杆菌介导法将BCL和RIP 2个与细胞凋亡有关的基因分别导人了小麦品种扬麦10号和Bobwhite,转化效率分别为1.60%和1.25%。Southern检测结果表明,细胞凋亡基因已稳定整合到小麦染色体上,多数植株为单拷贝整合。Northern检测结果表明,细胞凋亡基因能够在小麦遗传背景中高水平转录为RNA。转基因植株T1代分离比例为2.11~2.33:1,表明外源基因在后代中能够稳定遗传。BCL转基因植株和RIP转基因植株对赤霉病均表现出一定抗性,其中BCL-20、BCL-21、RIP-8、RIP-18等15个T1代植株的小穗发病率为5.6%~16.1%,可望进一步培育抗赤霉病小麦新材料。 | 叶兴国 Shirley Sato 徐惠君 杜丽璞 黄益洪 陆维忠 Tom Clemente | 2005 | 作物学报2005，31，11： | 12 |
| 4 | 北京女性骨质疏松患病率调查分析显示文摘目的:利用放射吸收(Radiographic absoptiometry,RA)方法测定手指骨密度,了解北京女性骨质疏松症发病率。方法:选取北京地区41岁以上健康女性2841例,研究对象详细填写健康调查表,排除因继发性骨病或服用影响骨代谢药物的病例。用美国CompuMed公司OsteoGram-2000骨密度仪测定所有对象非优势手的第2、3、4指中节指骨(MP骨密度值,按10岁一个年龄组分组,用OsteoGram软件进行统计分析。结果:41岁以上女性的40.1%患有骨量减低,24.6%患有骨质疏松。结论:RA测量北京地区健康女性骨量丢失规律与我国部分地区用DXA或SPA方法所得结果相似。 | 王建华 张智海 钟平 王泉来 Shirley X.Bi Lauai Al-Dayeh | 2008 | 航空航天医药2008，19，2： | 11 |
| 5 | Utility of magnetic resonance imaging in anorectal disease显示文摘Imaging of both benign and malignant anorectal diseases has traditionally posed a challenge to clinicians, and as a result history and physical exam have been relied on heavily. CT scanning and endorectal ultrasound have become popular in assessment of anatomy and staging of tumors, but have limitations. Magnetic resonance imaging (MRI) has the capability to fill in the gaps left open by more conventional imaging modalities and continues to be promising as the definitive imaging technique in the pelvis, especially with advancement of emerging technologies in this field. A comprehensive review of this topic has been undertaken. Anorectal disease is divided into three broad categories: cancer, fistula/abscess, and pelvic floor disorders. A review of the literature is performed to evaluate the use of MRI and other imaging modalities in these three areas. Preoperative imaging is useful in the evaluation of all three areas of anorectal disease. MRI is an effective tool in delineating anatomy and, when correlating with the specific clinical scenario, is an effective adjunct in clinical decision-making in order to optimize outcome. MRI continues to be a promising and novel approach to imaging various afflictions of the anorectum and the pelvic floor. Its role is more well-established in some areas than in others, and there are still signif icant limitations. As technology advances, MRI will shed more light on a complex anatomical area. | Loren Berman Gary M Israel Shirley M McCarthy Jeffrey C Weinreb Walter E Longo | 2007 | World Journal of Gastroenterology2007，13，23： | 11 |
| 6 | 种植模式对旱地玉米光合特性和产量的影响显示文摘为进一步优化陇中半干旱区玉米高产栽培技术,提高有限水土资源利用效率,2014—2015年采用田间试验,研究了玉米马铃薯间作和单作下全膜沟垄作、全膜平作和露地平作对玉米抽雄期光合速率(Pn)、蒸腾速率(Tr)、气孔导度(Gs)、胞间CO_2浓度(Ci)及子粒产量的影响。结果表明:(1)不同种植模式下玉米抽雄期叶片Pn和Tr的日变化均呈先升后降的单峰曲线,峰值出现在中午12∶00左右,全膜沟垄作高于平作和露地,间作高于单作;全膜沟垄作玉米间作马铃薯下的玉米Pn、Tr和Gs均最高。(2)全膜沟垄作、全膜平作和露地平作的玉米间作马铃薯,土地当量比(LER)均大于1,以全膜沟垄作玉米间作马铃薯优势最为突出,其间作下玉米子粒产量为5 451.35kg·hm^(-2),较露地间作提高了66.00%,特别是在降水量偏少的年份,增产效果更明显。因此,在陇中雨养农业区进一步提高玉米产量和水土资源利用效率,引入间作是可行的,全膜沟垄作玉米间作马铃薯是较佳的种植模式。 | 任金虎 谢军红 李玲玲 Lamptey Shirley Stephen Yeboah 张明君 高海强 | 2017 | 干旱地区农业研究2017，35，5： | 10 |
| 7 | 鸡球虫病的免疫控制显示文摘 | Shirley M.W 蔡建平 谢明权 | 2003 | 中国家禽2003，25，7： | 9 |
| 8 | Genome-Wide Analysis of Histone Modifications: H3K4me2, H3K4me3, H3K9ac, and H3K27ac in Oryza sativa L. Japonica显示文摘当以前的研究证明了 histone 修正能由调整基因抄写影响工厂生长和开发时,在这些修正和基因表示之间的关系的知识仍然是有限的。这研究使用了高产量的定序跟随的染色质 immunoprecipitation (ChIP-Seq ) ,到调查四 histone 修正的染色体宽的分发:H3K4 (H3K4me2 和 H3K4me3 ) 和在 Oryza sativa L 的 H3K9 和 H3K27 (H3K9ac 和 H3K27ac ) 的 acylation 的 di 和 trimethylation。装饰用的梨树。由分析出版 DNase-Seq 数据,这研究探索了 DNase 过分敏感(DH ) 沿着米饭染色体的地点。histone 标记主要在通用区域出现了并且在基因的抄写开始地点(TSS ) 附近被充实。这分析证明四 histone 修正和 DH 地点都与活跃抄写被联系。而且,四 histone 修正与答应被用来在米饭基因注解预言失踪的基因的特征的抄本 regionsa 是高度并发的。预言被试验性地证实二的抄写预言了失踪的基因进一步验证。而且,顺序主题分析被构造以便识别 DH 地点和许多通常认为的抄写因素绑定地点。 | Zhou Du Hui Li Qiang Wei Xin Zhao Chunchao Wang Qilin Zhu Xin Yi Wenying Xua X. Shirley Liu Weiwei Jin Zhen Su | 2013 | Molecular Plant2013，6，5： | 8 |
| 9 | 耕作方式与施肥对旱农区粮饲兼用玉米耗水特性和干物质积累的影响显示文摘采用大田试验,在全膜双垄沟播技术的基础上,比较研究了4种耕作方式(T1:传统翻耕,T2:旋耕,T3:深松耕,T4:免耕)搭配2种施氮水平(N2:施纯氮200 kg·hm^(-2),N3:施纯氮300 kg·hm^(-2))下对玉米耗水特性和干物质积累的影响。结果表明:与施氮量相比,耕作方式对耗水量和土壤贮水消耗量影响显著;深松耕降低了玉米播种至拔节期的阶段耗水量,提高了拔节至灌浆期的耗水量及其占总耗水量的比例;播种至拔节期深松耕较传统翻耕0~30 cm土层的贮水消耗量减少了35.3%,拔节至开花期和开花至灌浆期,30~110 cm土层贮水消耗量增加了54.2%和20.5%;玉米不同生育时期各器官的干物质积累量都表现为深松耕+施纯氮300 kg·hm^(-2)最高,是生物产量和籽粒产量最高的处理,深松耕+施纯氮300 kg·hm^(-2)和深松耕+施纯氮200 kg·hm^(-2)处理间差异不显著;免耕+施纯氮200 kg·hm^(-2)处理的水分利用效率最高,与深松耕+施纯氮300 kg·hm^(-2)处理相比差异不显著。在综合考虑高产以及对水分和肥料的高效利用的条件下,基于全膜双垄沟播技术,深松耕+施纯氮200 kg·hm^(-2)是陇中旱农区适宜的生产方式。 | 张明君 李玲玲 谢军红 任金虎 Lamptey Shirley | 2018 | 干旱地区农业研究2018，36，1： | 8 |
| 10 | Ba(Mg_(1/3)Nb_(2/3))O_3电子结构第一性原理计算及光学性能研究显示文摘Ba(Mg1/3Nb2/3)O3(BMN)复合钙钛矿陶瓷具有高介电常数和高品质因子等介电性能,预示了其在光学领域的应用前景.本文采用第一性原理方法计算了BMN的电子结构,对其本征光学性能进行分析和预测.对固相合成六方相BMN的XRD测试结果进行Rietveld精修(加权方差因子Rwp=6.73%,方差因子Rp=5.05%),在此基础上建立晶体结构模型并对其进行几何优化.运用基于密度泛函理论(DFT)的平面波赝势方法,对六方相BMN晶体模型的能带、态密度和光学性质进行理论计算.结果表明BMN的能带结构为间接带隙,禁带宽度Eg=2.728 e V.Mg-O和Ba-O以离子键结合为主,Nb-O以共价键结合为主,费米面附近的能带主要由O-2p和Nb-4d态电子占据,形成了d-p轨道杂化.修正带隙后,计算了BMN沿[100]和[001]方向上的复介电函数、吸收系数和反射率等光学性质.结果表明,BMN近乎光学各向同性,在可见光区,其本征透过率为77% | 沈杰 魏宾 周静 Shen Shirley Zhiqi 薛广杰 刘韩星 陈文 | 2015 | 物理学报2015，64，21： | 6 |
| 11 | 集成案例推理综述显示文摘如何将智能方法和智能技术与案例推理集成来提高案例推理的性能是专家系统研发的一个重要课题。随着计算机科学的飞速发展,智能方法与智能技术与案例推理的集成呈现了新特点。针对近几年案例推理与智能方法和智能技术集成的新进展进行了综述,分析了案例推理与智能方法和智能技术集成的原因,归纳了所应用的集成模型的类别和在案例推理中的具体应用,总结了案例推理与智能方法和智能技术集成的优势;根据当前研究现状,探讨了集成案例推理未来发展的趋势和新方法。 | 张佰尚 李向阳 王铁男 Shirley Gregor | 2016 | 计算机应用研究2016，33，3： | 6 |
| 12 | Therapeutic induction of autophagy to modulate neurodegenerative disease progression显示文摘 | Warren E HOCHFELD Shirley LEE David C RUBINSZTEIN | 2013 | Acta Pharmacologica Sinica2013，34，5： | 6 |
| 13 | Superior growth performance in broiler chicks fed chelated compared to inorganic zinc in presence of elevated dietary copper显示文摘Background: The goal of this study was to compare the antagonism of elevated dietary Cu(250 mg/kg) from Cu SO4 on three different Zn sources(Zn SO4· H2O; [Zn bis(-2-hydroxy-4-(methylthio)butanoic acid)], Zn(HMTBa)2,a chelated Zn methionine hydroxy analogue; and Zn-Methionine), as measured using multiple indices of animal performance in ROSS 308 broilers.Methods: Three experiments were conducted in broiler chicks fed a semi-purified diet. Al birds were fed a Zn-deficient diet(8.5 mg/kg diet) for 1 wk, and then provided with the experimental diets for 2 wks.Results: Experiment 1 was a 2 × 2 factorial design with two levels of Cu(8 vs. 250 mg/kg diet from Cu SO4) and two Zn sources at 30 mg/kg [Zn SO4· H2 O vs. Zn(HMTBa)2]. Elevated Cu impaired growth performance only in birds fed Zn SO4.Compared to Zn SO4· H2 O, Zn(HMTBa)2improved feed intake(12 %; P < 0.001) and weight gain(12 %, P < 0.001) and the benefits were more pronounced in the presence of 250 mg/kg diet Cu. Experiment 2 was a dose titration of Zn SO4· H2 O and Zn(HMTBa)2at 30, 45, 60, and 75 mg/kg diet in the presence of 250 mg/kg Cu SO4. Feed:gain was decreased and tibia Zn was increased with increasing Zn levels from 30 to 75 mg/kg. Birds fed Zn(HMTBa)2consumed more food and gained more weight compared to birds fed Zn SO4, especially at lower supplementation levels(30 and45 mg/kg; interaction P < 0,05). Experiment 3 compared two organic Zn sources(Zn(HMTBa)2vs. Zn-Methionine)at 30 mg/kg with or without 250 mg/kg Cu SO4. No interactions were observed between Zn sources and Cu levels on performance or tissue mineral concentrations. High dietary Cu decreased weight gain(P < 0.01). Tibia Cu and liver Cu were significantly increased with 250 mg/kg dietary Cu supplementation(P < 0.01). No difference was observed between the two Zn sources.Conclusions: Dietary 250 mg/kg Cu significantly impaired feed intake and weight gain in birds fed Zn SO4· H2 O,but had less impact in birds fed Zn(HMTBa)2. No difference was observed between the two organic zinc sources.These results are consistent with the hypothesis that chelated organic Zn is better utilized than inorganic zinc in the presence of elevated Cu. | Junmei Zhao Robert B. Shirley Julia J. Dibner Karen J. Wedekind Frances Yan Paula Fisher Thomas R. Hampton Joseph L. Evans Mercedes Vazquez-Anon | 2016 | Journal of Animal Science and Biotechnology2016，7，4： | 6 |
| 14 | 连续14年保护性耕作对土壤总有机碳和轻组有机碳的影响显示文摘依托于2001年布设在陇中黄土高原半干旱雨养农业区的保护性耕作定位试验,于2014年测定了5种保护性耕作(免耕+秸秆覆盖NTS、免耕NT、传统耕作+秸秆翻埋TS、传统耕作+地膜覆盖TP和免耕+地膜覆盖NTP)和传统耕作T处理下小麦-豌豆双序列轮作中表层土壤(0~5、5~10、10~30 cm)总有机碳(SOC)和轻组有机碳(LFOC)在作物生育期前后的变化。结果表明:土壤总有机碳和轻组有机碳在土壤剖面上均随着土层深度的增加而降低;相比传统耕作T,NTS和TS处理能显著提高0~30 cm土层中SOC、LFOC的含量,在作物播种前较T分别提高了19.51%、64.58%和13.36%、42.08%,在收获后分别提高了28.00%、85.37%和18.61%、77.82%,而SOC、LFOC含量NT和TP处理与T处理间差异不显著;从作物播种前至收获后,各处理下0~30 cm土层SOC含量均有减小趋势,其中NTS和TS处理变化量最小,NT和TP处理加大了作物生育期间SOC和LFOC的消耗;LFOC可以灵敏地反应出土壤有机碳的变化。因此,在该区推行以免耕、秸秆覆盖为主的保护性耕作措施更有利于碳的积累和土壤质量的改善,促进该区农业的可持续发展。 | 刘杰 李玲玲 谢军红 邓超超 彭正凯 Yeboah Stephen Lamptey Shirley | 2017 | 干旱地区农业研究2017，35，1： | 6 |
| 15 | NiPdAu涂层的装配及封装的可靠性研究显示文摘为了迎接无铅时代的到来,不少半导体公司正趋向选用预镀镍-钯金穴NiPdAuppf雪涂层的引线框架,以简化生产工艺。但是,这种方法需要仔细考虑NiPdAu涂层上金焊点的质量、其与修剪成形工具的兼容性以及选择正确的原材料组合穴用于粘结芯片的环氧树脂和塑封材料雪,以确保在260℃下达到1级MSL穴潮湿度敏感等级雪。将探讨装配过程的特性,以保证金焊点牢固地焊接在NiPbAu涂层上;如何优化选用适当的材料组合;修剪成形工具的修改以避免涂层出现机械破坏;以及NiPdAu涂层的可焊性评估。 | Shirley Kang | 2004 | 电子工业专用设备2004，33，12： | 5 |
| 16 | The interplay of aging,genetics and environmental factors in the pathogenesis of Parkinson’s disease显示文摘Background:Parkinson’s disease(PD)is characterized by dopaminergic neuronal loss in the substantia nigra pars compacta and intracellular inclusions called Lewy bodies(LB).During the course of disease,misfoldedα-synuclein,the major constituent of LB,spreads to different regions of the brain in a prion-like fashion,giving rise to successive non-motor and motor symptoms.Etiology is likely multifactorial,and involves interplay among aging,genetic susceptibility and environmental factors.Main body:The prevalence of PD rises exponentially with age,and aging is associated with impairment of cellular pathways which increases susceptibility of dopaminergic neurons to cell death.However,the majority of those over the age of 80 do not have PD,thus other factors in addition to aging are needed to cause disease.Discovery of neurotoxins which can result in parkinsonism led to efforts in identifying environmental factors which may influence PD risk.Nevertheless,the causality of most environmental factors is not conclusively established,and alternative explanations such as reverse causality and recall bias cannot be excluded.The lack of geographic clusters and conjugal cases also go against environmental toxins as a major cause of PD.Rare mutations as well as common variants in genes such as SNCA,LRRK2 and GBA are associated with risk of PD,but Mendelian causes collectively only account for 5%of PD and common polymorphisms are associated with small increase in PD risk.Heritability of PD has been estimated to be around 30%.Thus,aging,genetics and environmental factors each alone is rarely sufficient to cause PD for most patients.Conclusion:PD is a multifactorial disorder involving interplay of aging,genetics and environmental factors.This has implications on the development of appropriate animal models of PD which take all these factors into account.Common converging pathways likely include mitochondrial dysfunction,impaired autophagy,oxidative stress and neuroinflammation,which are associated with the accumulation and spread of misfoldedα-synuclein and neurodegeneration.Understanding the mechanisms involved in the initiation and progression of PD may lead to potential therapeutic targets to prevent PD or modify its course. | Shirley Yin-Yu Pang Philip Wing-Lok Ho Hui-Fang Liu Chi-Ting Leung Lingfei Li Eunice Eun Seo Chang David Boyer Ramsden Shu-Leong Ho | 2019 | Translational Neurodegeneration2019，8，1： | 5 |
| 17 | 基于钢轨应变监测的多跨铁路简支梁桥桥墩差异沉降识别显示文摘旨在建立基于钢轨应变监测的多跨铁路简支梁桥桥墩差异沉降识别方法。首先,针对对称转角沉降模式的分析表明钢轨与梁体竖向变形具有一致性,为建立沉降致钢轨应变与桥墩差异沉降关系方程奠定了基础。其次,采用空间梁单元、实体单元、弹簧单元,结合主从自由度耦合技术,建立了某4跨铁路简支梁桥的钢轨-梁体-桥墩有限元模型。基于该模型的分析表明,轨底可测应变与桥墩差异沉降间存在显著正比例线性关系且应变绝对变化量可测。然后,通过理论推导,得到了沉降致钢轨应变与简支梁桥桥墩差异沉降量之间的解析表达式。解析式物理意义明确,表达形式简单。最后,对解析解与数值解进行了对比分析,两者变化趋势相同,解析解因未考虑外围钢轨约束、下部梁体弯曲变形等,所得结果略大于数值解。 | 王少杰 徐赵东 李舒 Shirley Dyke | 2016 | 铁道学报2016，38，3： | 5 |
| 18 | 磁流变阻尼器的半主动控制及实时混合模拟试验研究显示文摘磁流变阻尼器作为一种比较典型的半主动控制元件,具有构造简单、响应速度快、耐久性好、阻尼力大且连续可调等优点。即使地震中能源中断,磁流变阻尼器仍可以作为被动耗能装置继续工作发挥作用,可靠性高。设计合理有效的磁流变阻尼器半主动控制方法,对于整体结构的减震效果尤其重要。提出一种改进的磁流变阻尼器的半主动控制策略-改进的Bang-Bang控制策略,对装有磁流变阻尼器的减震控制3层框架结构进行了一系列的实时混合模拟试验,对多种半主动控制方法下的振动控制效果进行试验分析。试验结果表明:磁流变阻尼器对框架结构的减震效果显著,并验证了提出的磁流变阻尼器半主动控制策略的有效性。 | 李歆 吕西林 Shirley Dyke | 2018 | 世界地震工程2018，34，4： | 5 |
| 19 | Fluxing Agents on Ceramification of Composites of MgO-Al2O3-SiO2/Boron Phenolic Resin显示文摘Fluxing agents of zinc borate, antimony oxide, galss frit A and glass frit B, with different melting or softening point temperatures, were added into MgO-Al_2O_3-SiO_2/boron phenol formaldehyde resin(MAS/BPF) composites to lower the formation temperature of eutectic liquid phase and promote the ceramification of ceramifiable composites. The effects of fluxing agents on the thermogravimetric properties, phase evolution, and microstructure evolution of MAS/BPF composites were characterized by TG-DSC, XRD and SEM analyses. The results reveal that the addition of a fluxing agent highly reduces the decomposition rate of MAS/BPF composites. Fluxing agents lower the formation temperatures of liquid phases of ceramifiable MAS/BPF composites obviously, and then promote the ceramification and densification process. The final residues of composites are ceramic surrounded by large amount of glass phases. | 石敏先 CHEN Xia FAN Shanshan SHEN Shirley LIU Tianxiang 黄志雄 | 2018 | Journal of Wuhan University of Technology(Materials Science)2018，33，2： | 4 |
| 20 | Needle track seeding:A real hazard after percutaneous radiofrequency ablation for colorectal liver metastasis显示文摘Neoplastic needle track seeding following percutaneous radiofrequency ablation(RFA)of secondary liver tumors is exceedingly rare.Reports on cutaneous tumor seeding after percutaneous RFA for colorectal liver metastasis are even rarer in the literature.Here we report a case of a 46-year-old female who developed an ulcerating skin lesion along the needle track of a previous percutaneous RFA site around 6 mo after the procedure.The previous RFA was performed by the LeVeen needle for a secondary liver tumor from a primary rectal cancer.The diagnosis of secondary skin metastasis was confirmed by fine needle aspiration cytology.The lesion was successfully treated with wide local excision.We believe that tumor seeding after percutaneous RFA in our patient was possibly related to its unfavorable subcapsular location and the use of an expansion-type needle.Hence,prophylactic ablation of the needle track should be performed whenever possible.Otherwise,alternative routes of tumor ablation such as laparoscopic or open RFA should be considered. | Shirley Yuk-Wah Liu Kit-Fai Lee Paul Bo-San Lai | 2009 | World Journal of Gastroenterology2009，15，13： | 4 |
您所在的机构暂未开通此部分的功能，请联系管理员