4篇
您的检索式：作者名="SARA FASOLI"
|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | 13 C-galactose breath test and 13 C-aminopyrine breath test for the study of liver function in chronic liver disease显示文摘 | Edoardo G. Giannini Alberto Fasoli Paolo Borro Federica Botta Federica Malfatti Alessandra Fumagalli Emanuela Testa Simone Polegato Tiziana Cotellessa Sara Milazzo Domenico Risso Roberto Testa | 2005 | Clinical Gastroenterology and Hepatology2005，，： | 1 |
| 2 | Bacterial composition of commercial probiotic products as evaluated by PCR-DGGE analysis显示文摘 | SARA FASOLI MARIA MAEZOTTO LUCIA RIZZOTTI | 2003 | International Journal of Food Microbiology2003，，82： | 1 |
| 3 | DNA-free genome editing in grapevine using CRISPR/Cas9 ribonucleoprotein complexes followed by protoplast regeneration显示文摘CRISPR/Cas9 genome editing technology can overcome many limitations of traditional breeding,offering enormous potential for crop improvement and food production.Although the direct delivery of Cas9-single guide RNA(sgRNA)ribonucleoprotein(RNP)complexes to grapevine(Vitis vinifera)protoplasts has been shown before,the regeneration of edited protoplasts into whole plants has not been reported.Here,we describe an efficient approach to obtain transgene-free edited grapevine plants by the transfection and subsequent regeneration of protoplasts isolated from embryogenic callus.As proof of concept,a single-copy green f luorescent protein reporter gene(GFP)in the grapevine cultivar Thompson Seedless was targeted and knocked out by the direct delivery of RNPs to protoplasts.CRISPR/Cas9 activity,guided by two independent sgRNAs,was confirmed by the loss of GFP f luorescence.The regeneration of GFP−protoplasts into whole plants was monitored throughout development,confirming that the edited grapevine plants were comparable in morphology and growth habit to wild-type controls.We report the first highly efficient protocol for DNA-free genome editing in grapevine by the direct delivery of preassembled Cas9-sgRNA RNP complexes into protoplasts,helping to address the regulatory concerns related to genetically modified plants.This technology could encourage the application of genome editing for the genetic improvement of grapevine and other woody crop plants. | Samaneh Najafi Edoardo Bertini Erica D’Incà Marianna Fasoli Sara Zenoni | 2023 | Horticulture Research2023，10，1： | 0 |
| 4 | A molecular phenology scale of grape berry development显示文摘Fruit growth and development consist of a continuous succession of physical,biochemical,and physiological changes driven by a genetic program that dynamically responds to environmental cues.Establishing recognizable stages over the whole fruit lifetime represents a fundamental requirement for research and fruit crop cultivation.This is especially relevant in perennial crops like grapevine(Vitis vinifera L.)to scale the development of its fruit across genotypes and growing conditions.In this work,molecular-based information from several grape berry transcriptomic datasets was exploited to build a molecular phenology scale(MPhS)and to map the ontogenic development of the fruit.The proposed statistical pipeline consisted of an unsupervised learning procedure yielding an innovative combination of semiparametric,smoothing,and dimensionality reduction tools.The transcriptomic distance between fruit samples was precisely quantified by means of the MPhS that also enabled to highlight the complex dynamics of the transcriptional program over berry development through the calculation of the rate of variation of MPhS stages by time.The MPhS allowed the alignment of time-series fruit samples proving to be a complementary method for mapping the progression of grape berry development with higher detail compared to classic time-or phenotype-based approaches. | Giovanni Battista Tornielli Marco Sandri Marianna Fasoli Alessandra Amato Mario Pezzotti Paola Zuccolotto Sara Zenoni | 2023 | Horticulture Research2023，10，5： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员